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The map label for this gene is mscL [H]

Identifier: 37520642

GI number: 37520642

Start: 1149537

End: 1149983

Strand: Direct

Name: mscL [H]

Synonym: glr1073

Alternate gene names: 37520642

Gene position: 1149537-1149983 (Clockwise)

Preceding gene: 37520638

Following gene: 37520643

Centisome position: 24.67

GC content: 56.15

Gene sequence:

>447_bases
ATGTTTATCTCGACCATTGGAGGTTCCAAGATGCTGGACGGATTCAAACAATTTTTGCTGCGGGGGAATGTCGTCGACTT
GGCCGTGGGCGTGGTCATCGGTGCCGCCTTCGGAAAGATCGTCGAAACCCTGGTTTCAGGATTCATCACCCCGCTGATTG
CCGCCATCGGCGGCCAGCCGGATTTCTCGGGTCTCTACTTCACGATCAACAACAGCAAGTTCATGTACGGCCAGTTCATC
AACGCGATCATCTCGTTTTTGATCATCGCGGCGGTGGTCTACTTTTTGATCGTGCTGCCGCTCAACGCCCTGATTGCGCG
GGCGCACAAGGAACCGTCCCCCGACCCGACCACCAAGAAATGTCCCGAATGCACCAGCGAAATTGCGATCGATGCGCGCC
GCTGCCCTTTTTGTACTTCGGTGCTGGTGGGTTCGCCGACGAAGTGA

Upstream 100 bases:

>100_bases
TCCCCGGATGGGCGACGTATACTCACCCACAGGCGATACAGCTCGGTAAAGCAACGCTTCCATACAAGAAAAGCAGCCGA
CTTTCTGAGATTATTCACGT

Downstream 100 bases:

>100_bases
GCCGCAGCATTCCCGAATTTTTTACCGTCAGCCGGCAACTATGGAGCTGTCTCCCGTCCGCGATCAAAAAAGAAAACGTC
GCGCGCCGAGCCGATCGGGT

Product: large-conductive mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MFISTIGGSKMLDGFKQFLLRGNVVDLAVGVVIGAAFGKIVETLVSGFITPLIAAIGGQPDFSGLYFTINNSKFMYGQFI
NAIISFLIIAAVVYFLIVLPLNALIARAHKEPSPDPTTKKCPECTSEIAIDARRCPFCTSVLVGSPTK

Sequences:

>Translated_148_residues
MFISTIGGSKMLDGFKQFLLRGNVVDLAVGVVIGAAFGKIVETLVSGFITPLIAAIGGQPDFSGLYFTINNSKFMYGQFI
NAIISFLIIAAVVYFLIVLPLNALIARAHKEPSPDPTTKKCPECTSEIAIDARRCPFCTSVLVGSPTK
>Mature_148_residues
MFISTIGGSKMLDGFKQFLLRGNVVDLAVGVVIGAAFGKIVETLVSGFITPLIAAIGGQPDFSGLYFTINNSKFMYGQFI
NAIISFLIIAAVVYFLIVLPLNALIARAHKEPSPDPTTKKCPECTSEIAIDARRCPFCTSVLVGSPTK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=121, Percent_Identity=40.495867768595, Blast_Score=79, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 15819; Mature: 15819

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFISTIGGSKMLDGFKQFLLRGNVVDLAVGVVIGAAFGKIVETLVSGFITPLIAAIGGQP
CEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DFSGLYFTINNSKFMYGQFINAIISFLIIAAVVYFLIVLPLNALIARAHKEPSPDPTTKK
CCCCEEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
CPECTSEIAIDARRCPFCTSVLVGSPTK
CHHHHHHHHHCCCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MFISTIGGSKMLDGFKQFLLRGNVVDLAVGVVIGAAFGKIVETLVSGFITPLIAAIGGQP
CEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DFSGLYFTINNSKFMYGQFINAIISFLIIAAVVYFLIVLPLNALIARAHKEPSPDPTTKK
CCCCEEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
CPECTSEIAIDARRCPFCTSVLVGSPTK
CHHHHHHHHHCCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA