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The map label for this gene is topA [H]

Identifier: 37520589

GI number: 37520589

Start: 1095482

End: 1097443

Strand: Reverse

Name: topA [H]

Synonym: gvip136

Alternate gene names: 37520589

Gene position: 1097443-1095482 (Counterclockwise)

Preceding gene: 37520590

Following gene: 37520584

Centisome position: 23.56

GC content: 65.75

Gene sequence:

>1962_bases
ATGATGCGGCTATTGATTTGCGAAAGTCCAGGTAAGATCAAAACTTTTAAGGCAATTCTCGGCGCGGGCTGGGACGTGCA
GGCGTCTTTGGGTCATGTCATGGAGTTGGCCAACGACGGTGCCGATCACCTGGGGTTCGATGTCGGCCCCGAGGCGATAA
CCTGCCGCTACGTGCCCCGCGGCGAGCGCGGGATGGCGACGCTCAAGAAACTGCGCACAGCGGCGGCTAAGGCCCAGGAA
GTGTATCTAGCCACCGACCCCGACCGTGAAGGGGAAGCGATTGCCTGGCATTTGGCACGGGAGTTGCGCCTCAAATCCCC
CCGGCGCATCCGCTGTACTCAGATCACTGAGGGAGCCGTGCGCACAGCCCTGCAATCACCCGGTCGCCTGGATATGGACC
TGGTGAGCGCCCAGCGCGCCCGGCAGTGCCTCGACAAGCTGGTGGGCTACAAAGTTTCGCCCCTGCTCTGGAATGCGACC
GGCGGCAAGAGCGCGGGCCGGGTCCAAAGTGCGACGCTGCACTTTGTGTGCGAGCGCGAACGGGAGATTATCGCCTTTGT
GCCGGTCGATTACTGGTCGGTGTGGGTCGAGTACGCCCCCGGCTGGCGGGCTTACTACCAAGGGGACGTGCAGGAGACGC
CCGAGTCGACCGAGGCGACCGACGACGCCAAATCGAACAAAGAAGCGCCCCCCGCCGAATCGACCCGGGTGCTGAGCGAA
GCGGAGGCCGACCGGCTGGTCGCCTTGGCGCGCAGCCACCCCCACGCTGTCGAGCGCGTCGAGCGGCGCACCGTTACGAA
GATCCCCCCGGCCCCCTTCACCACCAGTACCCTCCAGCAGGCCGCTGGGGCATTGCTCAGCTACAACCCCGAGCGCACGA
TGGGTATCGCCCAGCAGCTCTACGAAGGCATCGACCTGCCCACCGGCCGCAAGGGGCTCATTACCTACATGCGCACCGAC
AGCGTCGAAGTGGCCCCCGAATTTGTCGAACAGGCGCGCACCTACCTGCAGATCAATGACCCCGACAATCTGCCCGCCCG
CAGCGCCACGCACCGCTCCCGCGCAGGTGCCCAGCAGGCCCATGAAGCGATCCGCCCCACCGACGTCAGCTTGACGCCGC
GCACGATCCGAGCGCACCTGAGCGAGGAGCAACTGCGGCTTTACGACATTGTCTGGCGGCGGGCGGTGGCTTCGCAGTGC
GCCCCGGCCCGCCTGGCCAAGACAAAGATCTTGACCCGCTCCGGCCCGGTGCACTGGCAGGCTCTGGGCATGACCGTCGC
CTTCGCGGGCTACACGCGCTACTGGAACGACCTGGAGGCGGCCCTGGCCCTACCCGCCCTCGAAGCCGGACAGAACCTGG
AACTGCTGCGCTCGGCCCACGAAAAAAAGCGCACCCAACCGCCGCCGCGCTACAGCGAGCCGAAGCTGGTGCAGCTGATG
GAGCGCAAAGGGGTCGGGCGGCCCTCGACCTACGCTTCGACGATCAAAACCCTCAAAGAGCGCGCCTACGTCGAACTGCA
GGGCCGCGCGCTGGTGCCCACCGAGCTGGGTCTGGCCACCGACGCGCTGCTCGGGCGCACGCTGCCGGAACTGGTCGACA
GCGCCTTTACCGCCCGGATGGAGACGGGCCTCGATGCGATTGCCGCAAGCCGCGAGCCCTGGGAAAAATATTTGATCGGC
TGGAACACAGACTATCTGGTGCCGGCGGTGGCGCGCGCCCGCGCGGCGATTGCGGCGGAATTTCCGAAGCGTCCGGCCGC
TCCACGCACAGCCCAAGCACCGCAGATTTCGCGCACCCCCTGTCCCCGTTGCCGCCAGGCGCTCTCGAAGGTACCCTCCA
AAAAAGTCAAGCGGGGCTACTTTCTCAAGTGCCCCGCCTGTGCGGATTTGGTGATGTTCTGGAACCCCTGGCGCAAAATC
TGGGAGCTTCCCAAGCCCAAAACGGACCCGCAAACTCCCTGA

Upstream 100 bases:

>100_bases
AAACAAGATGCAAGATATAAATTTTGTATAATCCTAACAACATCTAGATCAACAACTTAATTTTGCGGGACTATCGCGCT
GTTCGGGTGTAAATTTGTGA

Downstream 100 bases:

>100_bases
CACTGCACCCCGAACCGGTCCCTACGCCGAGGTCGCTACAGCGAGCGGCAAACTGGTCACAGGAGCCTCCAGCGGCGTCT
GCAGGAGCACCTGAATGAGC

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 653; Mature: 653

Protein sequence:

>653_residues
MMRLLICESPGKIKTFKAILGAGWDVQASLGHVMELANDGADHLGFDVGPEAITCRYVPRGERGMATLKKLRTAAAKAQE
VYLATDPDREGEAIAWHLARELRLKSPRRIRCTQITEGAVRTALQSPGRLDMDLVSAQRARQCLDKLVGYKVSPLLWNAT
GGKSAGRVQSATLHFVCEREREIIAFVPVDYWSVWVEYAPGWRAYYQGDVQETPESTEATDDAKSNKEAPPAESTRVLSE
AEADRLVALARSHPHAVERVERRTVTKIPPAPFTTSTLQQAAGALLSYNPERTMGIAQQLYEGIDLPTGRKGLITYMRTD
SVEVAPEFVEQARTYLQINDPDNLPARSATHRSRAGAQQAHEAIRPTDVSLTPRTIRAHLSEEQLRLYDIVWRRAVASQC
APARLAKTKILTRSGPVHWQALGMTVAFAGYTRYWNDLEAALALPALEAGQNLELLRSAHEKKRTQPPPRYSEPKLVQLM
ERKGVGRPSTYASTIKTLKERAYVELQGRALVPTELGLATDALLGRTLPELVDSAFTARMETGLDAIAASREPWEKYLIG
WNTDYLVPAVARARAAIAAEFPKRPAAPRTAQAPQISRTPCPRCRQALSKVPSKKVKRGYFLKCPACADLVMFWNPWRKI
WELPKPKTDPQTP

Sequences:

>Translated_653_residues
MMRLLICESPGKIKTFKAILGAGWDVQASLGHVMELANDGADHLGFDVGPEAITCRYVPRGERGMATLKKLRTAAAKAQE
VYLATDPDREGEAIAWHLARELRLKSPRRIRCTQITEGAVRTALQSPGRLDMDLVSAQRARQCLDKLVGYKVSPLLWNAT
GGKSAGRVQSATLHFVCEREREIIAFVPVDYWSVWVEYAPGWRAYYQGDVQETPESTEATDDAKSNKEAPPAESTRVLSE
AEADRLVALARSHPHAVERVERRTVTKIPPAPFTTSTLQQAAGALLSYNPERTMGIAQQLYEGIDLPTGRKGLITYMRTD
SVEVAPEFVEQARTYLQINDPDNLPARSATHRSRAGAQQAHEAIRPTDVSLTPRTIRAHLSEEQLRLYDIVWRRAVASQC
APARLAKTKILTRSGPVHWQALGMTVAFAGYTRYWNDLEAALALPALEAGQNLELLRSAHEKKRTQPPPRYSEPKLVQLM
ERKGVGRPSTYASTIKTLKERAYVELQGRALVPTELGLATDALLGRTLPELVDSAFTARMETGLDAIAASREPWEKYLIG
WNTDYLVPAVARARAAIAAEFPKRPAAPRTAQAPQISRTPCPRCRQALSKVPSKKVKRGYFLKCPACADLVMFWNPWRKI
WELPKPKTDPQTP
>Mature_653_residues
MMRLLICESPGKIKTFKAILGAGWDVQASLGHVMELANDGADHLGFDVGPEAITCRYVPRGERGMATLKKLRTAAAKAQE
VYLATDPDREGEAIAWHLARELRLKSPRRIRCTQITEGAVRTALQSPGRLDMDLVSAQRARQCLDKLVGYKVSPLLWNAT
GGKSAGRVQSATLHFVCEREREIIAFVPVDYWSVWVEYAPGWRAYYQGDVQETPESTEATDDAKSNKEAPPAESTRVLSE
AEADRLVALARSHPHAVERVERRTVTKIPPAPFTTSTLQQAAGALLSYNPERTMGIAQQLYEGIDLPTGRKGLITYMRTD
SVEVAPEFVEQARTYLQINDPDNLPARSATHRSRAGAQQAHEAIRPTDVSLTPRTIRAHLSEEQLRLYDIVWRRAVASQC
APARLAKTKILTRSGPVHWQALGMTVAFAGYTRYWNDLEAALALPALEAGQNLELLRSAHEKKRTQPPPRYSEPKLVQLM
ERKGVGRPSTYASTIKTLKERAYVELQGRALVPTELGLATDALLGRTLPELVDSAFTARMETGLDAIAASREPWEKYLIG
WNTDYLVPAVARARAAIAAEFPKRPAAPRTAQAPQISRTPCPRCRQALSKVPSKKVKRGYFLKCPACADLVMFWNPWRKI
WELPKPKTDPQTP

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=595, Percent_Identity=23.0252100840336, Blast_Score=117, Evalue=4e-26,
Organism=Homo sapiens, GI10835218, Length=635, Percent_Identity=25.1968503937008, Blast_Score=109, Evalue=7e-24,
Organism=Escherichia coli, GI1787529, Length=656, Percent_Identity=33.6890243902439, Blast_Score=328, Evalue=5e-91,
Organism=Escherichia coli, GI1788061, Length=623, Percent_Identity=23.916532905297, Blast_Score=94, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI32563869, Length=661, Percent_Identity=23.6006051437216, Blast_Score=125, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17555378, Length=520, Percent_Identity=24.0384615384615, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323263, Length=511, Percent_Identity=24.0704500978474, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24585251, Length=601, Percent_Identity=25.4575707154742, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 72587; Mature: 72587

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRLLICESPGKIKTFKAILGAGWDVQASLGHVMELANDGADHLGFDVGPEAITCRYVPR
CEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCCCEEEEECCC
GERGMATLKKLRTAAAKAQEVYLATDPDREGEAIAWHLARELRLKSPRRIRCTQITEGAV
CCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHH
RTALQSPGRLDMDLVSAQRARQCLDKLVGYKVSPLLWNATGGKSAGRVQSATLHFVCERE
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHEEEEEECC
REIIAFVPVDYWSVWVEYAPGWRAYYQGDVQETPESTEATDDAKSNKEAPPAESTRVLSE
CCEEEEECCHHHHHHHHCCCCCCEEEECCHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AEADRLVALARSHPHAVERVERRTVTKIPPAPFTTSTLQQAAGALLSYNPERTMGIAQQL
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
YEGIDLPTGRKGLITYMRTDSVEVAPEFVEQARTYLQINDPDNLPARSATHRSRAGAQQA
HHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHCCHHHH
HEAIRPTDVSLTPRTIRAHLSEEQLRLYDIVWRRAVASQCAPARLAKTKILTRSGPVHWQ
HHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEE
ALGMTVAFAGYTRYWNDLEAALALPALEAGQNLELLRSAHEKKRTQPPPRYSEPKLVQLM
EHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHH
ERKGVGRPSTYASTIKTLKERAYVELQGRALVPTELGLATDALLGRTLPELVDSAFTARM
HHCCCCCCHHHHHHHHHHHHHHEEEECCCEECCHHHCHHHHHHHHHHHHHHHHHHHHHHH
ETGLDAIAASREPWEKYLIGWNTDYLVPAVARARAAIAAEFPKRPAAPRTAQAPQISRTP
HHCHHHHHCCCCCHHHHEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
CPRCRQALSKVPSKKVKRGYFLKCPACADLVMFWNPWRKIWELPKPKTDPQTP
CHHHHHHHHHCCHHHHCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MMRLLICESPGKIKTFKAILGAGWDVQASLGHVMELANDGADHLGFDVGPEAITCRYVPR
CEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCCCEEEEECCC
GERGMATLKKLRTAAAKAQEVYLATDPDREGEAIAWHLARELRLKSPRRIRCTQITEGAV
CCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHH
RTALQSPGRLDMDLVSAQRARQCLDKLVGYKVSPLLWNATGGKSAGRVQSATLHFVCERE
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHEEEEEECC
REIIAFVPVDYWSVWVEYAPGWRAYYQGDVQETPESTEATDDAKSNKEAPPAESTRVLSE
CCEEEEECCHHHHHHHHCCCCCCEEEECCHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AEADRLVALARSHPHAVERVERRTVTKIPPAPFTTSTLQQAAGALLSYNPERTMGIAQQL
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
YEGIDLPTGRKGLITYMRTDSVEVAPEFVEQARTYLQINDPDNLPARSATHRSRAGAQQA
HHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHCCHHHH
HEAIRPTDVSLTPRTIRAHLSEEQLRLYDIVWRRAVASQCAPARLAKTKILTRSGPVHWQ
HHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEE
ALGMTVAFAGYTRYWNDLEAALALPALEAGQNLELLRSAHEKKRTQPPPRYSEPKLVQLM
EHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHH
ERKGVGRPSTYASTIKTLKERAYVELQGRALVPTELGLATDALLGRTLPELVDSAFTARM
HHCCCCCCHHHHHHHHHHHHHHEEEECCCEECCHHHCHHHHHHHHHHHHHHHHHHHHHHH
ETGLDAIAASREPWEKYLIGWNTDYLVPAVARARAAIAAEFPKRPAAPRTAQAPQISRTP
HHCHHHHHCCCCCHHHHEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
CPRCRQALSKVPSKKVKRGYFLKCPACADLVMFWNPWRKIWELPKPKTDPQTP
CHHHHHHHHHCCHHHHCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]