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The map label for this gene is gatA [H]

Identifier: 37520530

GI number: 37520530

Start: 1020621

End: 1022447

Strand: Direct

Name: gatA [H]

Synonym: glr0961

Alternate gene names: 37520530

Gene position: 1020621-1022447 (Clockwise)

Preceding gene: 37520524

Following gene: 37520534

Centisome position: 21.91

GC content: 68.58

Gene sequence:

>1827_bases
ATGCCTTCGCCGTCCTGGAGTCTTGACCTGCGGGCGCTGGCGGCGCGCTACCGGTCGGGGGAGGCCACGCCCGCAGGGGT
GGTGGCCGAGGTCTACGAGCGCATTTCCCGCTACGCGGATCCGGCCGTCTGGATTCACCTGCTGCCGATGGAGCGGGTGC
TGGAGGGAGCCCACACCCTGGCAGGCCGCGACCCGGCGGGCCTGCCGCTCTACGGGGTACCTTTTGCCGTCAAGGACAAC
CTCGATCTGGCCGGGGTGCCCACCACCGCCGGCTGTCCCGGTTTCGCCTACGGGCCTGAAAAGACGGCTCCGGTCGTCGA
GCGGTTGATGGCCGCCGGGGCGATCCCGGTCGGCAAGACCAACCTCGATCAGTTCGCCACCGGTCTGGTGGGCACCCGCA
CGCCCTACGGCGTCTGCCGCAACCCGTTCGACCGCCGCTACCTGCCCGGCGGTTCCAGTGCGGGTTCGGCGGTGGCGGTG
GCGGCGGGGCTGGTGAGCTTTGCCCTGGGCACCGACACCGCCGGGTCGGGGCGGGTTCCGGCCGCCTTTACCAACACCGT
CGGGCTCAAGCCCACCCGGGGTCTTTTGAGTACCCGCGGTCTGGTGCCGGCGGTGCGCAGCCTCGATTGCGTGTCGATCT
TCGCCCTGACTTGCGAAGATGCCGGGGCGGTGTTGTCGGTGGCGGCGGGCTTCGACCCGGACGATCCGTTTTCGCGCCGG
GCGCCCGCTGTCGAGACTCCGTCTTTTGACCGCCTGCGCGTCGGCGTGCCCGAGACCGACTATCTCGAATTTTTCGGCGA
CCGCGCGGCGCAGGGTCGCTACCGCGCGGCCCTCGCTCGACTGGAAGCCCTGGGCTGCGAACTGATCACCATCGATTTCG
GGCCGTTTGCCGACACTGCCCGGATGCTTTACGGCGGGCCGTGGGTGGCCGAACGCCTCGCGGCGGTGGGCGATTTTCTG
GAGCGCGAACCGGAATCCGTCCATCCGGTGGTGTACGAGATCATCGCGGGCGGGGCGCGCTACGACGCGGTGAGCGCCTA
CAAAGCCGCTTACCGGCTTGCCGGGTTGCGCCGCCTGAGCGAAGGGCAGTGGCAGCAGATGGACGTCCTGGCCCTTCCTA
CCACCGGCACGATCTATACCGTCGCCGAGGTGGAGCGCGATCCCATCGCCCTCAACGCCAACCTGGGCCTCTACACCAAC
TTCGTCAACCTGCTGGATCTGTGTGCGCTCGCCGTTCCGAGCGGCCCCGGCAAAGCCGGTCTGCCCACGGGGATCACCTT
CGTCGCCCCCGCCTGGCAAGAAACCCTGCTCTGTCGGCTGGGGGGTGTCTTCCATCCCTGGCCGGGGGCGACACTCGGCG
CAACCGGCCACCCGGTGCCCGTCCCCGAACCGATGGCAGACCCAGCGGATCGCGGGATCCAGCTCGCGGTGGTAGGCGCC
CACCTGAGCGGCCAGCCCCTCAATCACCAACTCACCGATCTGGGCGGTGCGCTGGTGCGCGCCTGCCGGACGGCTGCGCA
CTATCGGCTGTTTGCCCTCGTGGGTGAAAAAGTTCCGAAGCCCGGGTTGGTGCGCACCGACGACGGCAACGGTGCGGCTA
TCGAACTGGAAGTCTGGAAGCTTTCCGCCGAAGGGTTCGGCCGTTTCGTGGCCAATATCCCGCCGCCACTTGGGATCGGC
ACGCTGCTGCTTGAAGACGGGGAACAGGTGAAGGGTTTTCTGTGCGAAAGCTACGCCGTGGCAGGTGCGCCGGACATTAC
CGGCTTCGGCGGTTGGCGGGCCTATCTGGCCGCCAATGGCTCTTCAGCCGGGATCGCGCACGGGTAA

Upstream 100 bases:

>100_bases
CTCTTATCAGGAGCACAGGCCGGGCGGTGTATCGGCGGCTACGCTTGCCGATGGGTCCGGCCGGGCAAGATAGGCTGCAC
TTGCGTTTGGAAAGTTGCCG

Downstream 100 bases:

>100_bases
GGGCAGCGCCGGGACAAAACCCGGCGGGTGGCATTCGACGGGCAGCACGTCAATGACCACCAGACCGCCGCCGCCGATGG
GCCGGTAAGGCAGGTACACG

Product: allophanate hydrolase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 608; Mature: 607

Protein sequence:

>608_residues
MPSPSWSLDLRALAARYRSGEATPAGVVAEVYERISRYADPAVWIHLLPMERVLEGAHTLAGRDPAGLPLYGVPFAVKDN
LDLAGVPTTAGCPGFAYGPEKTAPVVERLMAAGAIPVGKTNLDQFATGLVGTRTPYGVCRNPFDRRYLPGGSSAGSAVAV
AAGLVSFALGTDTAGSGRVPAAFTNTVGLKPTRGLLSTRGLVPAVRSLDCVSIFALTCEDAGAVLSVAAGFDPDDPFSRR
APAVETPSFDRLRVGVPETDYLEFFGDRAAQGRYRAALARLEALGCELITIDFGPFADTARMLYGGPWVAERLAAVGDFL
EREPESVHPVVYEIIAGGARYDAVSAYKAAYRLAGLRRLSEGQWQQMDVLALPTTGTIYTVAEVERDPIALNANLGLYTN
FVNLLDLCALAVPSGPGKAGLPTGITFVAPAWQETLLCRLGGVFHPWPGATLGATGHPVPVPEPMADPADRGIQLAVVGA
HLSGQPLNHQLTDLGGALVRACRTAAHYRLFALVGEKVPKPGLVRTDDGNGAAIELEVWKLSAEGFGRFVANIPPPLGIG
TLLLEDGEQVKGFLCESYAVAGAPDITGFGGWRAYLAANGSSAGIAHG

Sequences:

>Translated_608_residues
MPSPSWSLDLRALAARYRSGEATPAGVVAEVYERISRYADPAVWIHLLPMERVLEGAHTLAGRDPAGLPLYGVPFAVKDN
LDLAGVPTTAGCPGFAYGPEKTAPVVERLMAAGAIPVGKTNLDQFATGLVGTRTPYGVCRNPFDRRYLPGGSSAGSAVAV
AAGLVSFALGTDTAGSGRVPAAFTNTVGLKPTRGLLSTRGLVPAVRSLDCVSIFALTCEDAGAVLSVAAGFDPDDPFSRR
APAVETPSFDRLRVGVPETDYLEFFGDRAAQGRYRAALARLEALGCELITIDFGPFADTARMLYGGPWVAERLAAVGDFL
EREPESVHPVVYEIIAGGARYDAVSAYKAAYRLAGLRRLSEGQWQQMDVLALPTTGTIYTVAEVERDPIALNANLGLYTN
FVNLLDLCALAVPSGPGKAGLPTGITFVAPAWQETLLCRLGGVFHPWPGATLGATGHPVPVPEPMADPADRGIQLAVVGA
HLSGQPLNHQLTDLGGALVRACRTAAHYRLFALVGEKVPKPGLVRTDDGNGAAIELEVWKLSAEGFGRFVANIPPPLGIG
TLLLEDGEQVKGFLCESYAVAGAPDITGFGGWRAYLAANGSSAGIAHG
>Mature_607_residues
PSPSWSLDLRALAARYRSGEATPAGVVAEVYERISRYADPAVWIHLLPMERVLEGAHTLAGRDPAGLPLYGVPFAVKDNL
DLAGVPTTAGCPGFAYGPEKTAPVVERLMAAGAIPVGKTNLDQFATGLVGTRTPYGVCRNPFDRRYLPGGSSAGSAVAVA
AGLVSFALGTDTAGSGRVPAAFTNTVGLKPTRGLLSTRGLVPAVRSLDCVSIFALTCEDAGAVLSVAAGFDPDDPFSRRA
PAVETPSFDRLRVGVPETDYLEFFGDRAAQGRYRAALARLEALGCELITIDFGPFADTARMLYGGPWVAERLAAVGDFLE
REPESVHPVVYEIIAGGARYDAVSAYKAAYRLAGLRRLSEGQWQQMDVLALPTTGTIYTVAEVERDPIALNANLGLYTNF
VNLLDLCALAVPSGPGKAGLPTGITFVAPAWQETLLCRLGGVFHPWPGATLGATGHPVPVPEPMADPADRGIQLAVVGAH
LSGQPLNHQLTDLGGALVRACRTAAHYRLFALVGEKVPKPGLVRTDDGNGAAIELEVWKLSAEGFGRFVANIPPPLGIGT
LLLEDGEQVKGFLCESYAVAGAPDITGFGGWRAYLAANGSSAGIAHG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=493, Percent_Identity=26.5720081135903, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI195972892, Length=167, Percent_Identity=34.7305389221557, Blast_Score=90, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17543272, Length=403, Percent_Identity=29.5285359801489, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17556264, Length=199, Percent_Identity=34.1708542713568, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=199, Percent_Identity=31.1557788944724, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17537465, Length=136, Percent_Identity=38.2352941176471, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=246, Percent_Identity=29.2682926829268, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=128, Percent_Identity=41.40625, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=128, Percent_Identity=41.40625, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=138, Percent_Identity=34.0579710144928, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=573, Percent_Identity=45.2006980802792, Blast_Score=419, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6323950, Length=269, Percent_Identity=31.2267657992565, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24648113, Length=495, Percent_Identity=29.6969696969697, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24652985, Length=409, Percent_Identity=25.6723716381418, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922090, Length=409, Percent_Identity=25.6723716381418, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24652981, Length=409, Percent_Identity=25.6723716381418, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24652983, Length=409, Percent_Identity=25.6723716381418, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24644968, Length=429, Percent_Identity=24.2424242424242, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21356731, Length=143, Percent_Identity=36.3636363636364, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45550774, Length=140, Percent_Identity=33.5714285714286, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648435, Length=140, Percent_Identity=33.5714285714286, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648437, Length=140, Percent_Identity=33.5714285714286, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648441, Length=140, Percent_Identity=33.5714285714286, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648439, Length=140, Percent_Identity=33.5714285714286, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI161078093, Length=415, Percent_Identity=23.6144578313253, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 63679; Mature: 63548

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPSWSLDLRALAARYRSGEATPAGVVAEVYERISRYADPAVWIHLLPMERVLEGAHTL
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHH
AGRDPAGLPLYGVPFAVKDNLDLAGVPTTAGCPGFAYGPEKTAPVVERLMAAGAIPVGKT
CCCCCCCCCEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
NLDQFATGLVGTRTPYGVCRNPFDRRYLPGGSSAGSAVAVAAGLVSFALGTDTAGSGRVP
CHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
AAFTNTVGLKPTRGLLSTRGLVPAVRSLDCVSIFALTCEDAGAVLSVAAGFDPDDPFSRR
EEECCCCCCCCCCHHHHHCCHHHHHHCCCEEEEEEEECCCCCCEEEEECCCCCCCCHHCC
APAVETPSFDRLRVGVPETDYLEFFGDRAAQGRYRAALARLEALGCELITIDFGPFADTA
CCCCCCCCCCEEEECCCCHHHHHHHCCHHCCCHHHHHHHHHHHCCCEEEEEECCCCHHHH
RMLYGGPWVAERLAAVGDFLEREPESVHPVVYEIIAGGARYDAVSAYKAAYRLAGLRRLS
HHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
EGQWQQMDVLALPTTGTIYTVAEVERDPIALNANLGLYTNFVNLLDLCALAVPSGPGKAG
CCCCCEEEEEEECCCCCEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCC
LPTGITFVAPAWQETLLCRLGGVFHPWPGATLGATGHPVPVPEPMADPADRGIQLAVVGA
CCCCCEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEC
HLSGQPLNHQLTDLGGALVRACRTAAHYRLFALVGEKVPKPGLVRTDDGNGAAIELEVWK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEEE
LSAEGFGRFVANIPPPLGIGTLLLEDGEQVKGFLCESYAVAGAPDITGFGGWRAYLAANG
ECHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECC
SSAGIAHG
CCCCCCCC
>Mature Secondary Structure 
PSPSWSLDLRALAARYRSGEATPAGVVAEVYERISRYADPAVWIHLLPMERVLEGAHTL
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHH
AGRDPAGLPLYGVPFAVKDNLDLAGVPTTAGCPGFAYGPEKTAPVVERLMAAGAIPVGKT
CCCCCCCCCEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
NLDQFATGLVGTRTPYGVCRNPFDRRYLPGGSSAGSAVAVAAGLVSFALGTDTAGSGRVP
CHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
AAFTNTVGLKPTRGLLSTRGLVPAVRSLDCVSIFALTCEDAGAVLSVAAGFDPDDPFSRR
EEECCCCCCCCCCHHHHHCCHHHHHHCCCEEEEEEEECCCCCCEEEEECCCCCCCCHHCC
APAVETPSFDRLRVGVPETDYLEFFGDRAAQGRYRAALARLEALGCELITIDFGPFADTA
CCCCCCCCCCEEEECCCCHHHHHHHCCHHCCCHHHHHHHHHHHCCCEEEEEECCCCHHHH
RMLYGGPWVAERLAAVGDFLEREPESVHPVVYEIIAGGARYDAVSAYKAAYRLAGLRRLS
HHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
EGQWQQMDVLALPTTGTIYTVAEVERDPIALNANLGLYTNFVNLLDLCALAVPSGPGKAG
CCCCCEEEEEEECCCCCEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCC
LPTGITFVAPAWQETLLCRLGGVFHPWPGATLGATGHPVPVPEPMADPADRGIQLAVVGA
CCCCCEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEC
HLSGQPLNHQLTDLGGALVRACRTAAHYRLFALVGEKVPKPGLVRTDDGNGAAIELEVWK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEEE
LSAEGFGRFVANIPPPLGIGTLLLEDGEQVKGFLCESYAVAGAPDITGFGGWRAYLAANG
ECHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECC
SSAGIAHG
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA