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The map label for this gene is trmD

Identifier: 37520458

GI number: 37520458

Start: 940972

End: 941655

Strand: Direct

Name: trmD

Synonym: glr0889

Alternate gene names: 37520458

Gene position: 940972-941655 (Clockwise)

Preceding gene: 37520454

Following gene: 37520459

Centisome position: 20.2

GC content: 64.33

Gene sequence:

>684_bases
ATGCGGATCGACATCGTCACGTTGTTTCCGGAGTTCTTTGTTTCCCCCCTGCAGTGCAGTTTGCTCGCAAGGGCGATAGC
AGGCGGAGTGTGCAACATCGCGATCACCAACCCGCGCGATTTTGCCGCCGACCGCTATCGGACGGTCGACGACACTCCCT
ACGGCGGCGGGGCGGGCATGGTGCTCAAACCCGAACCGCTCTTCGCGGCGGTCGAGTCGTTGCCGATCATCGCACCGCGG
GCGGTGATTCTGCTGACTCCCCAGGGCCGTCCTCTCAAGCAGGTGTTCCTGCGCTCGCTCGCGGCCGATTACGCCCAACT
GGTGCTTTTGTGCGGCCACTACGAAGGGGTGGATGAGCGGGTGCGCGCCCATCTGGCCACCCACGAAATTTCACTGGGGG
ATTTTGTGCTCACCGGCGGCGAGATCCCGGCTTTGGCGCTCATCGACGGGATTGTGCGCCTGTTGCCCGGTACTGTCGGC
AACCGCGCCTCCCTCGAATCGGAAAGTTTCGAGGACAACCTGCTTGAATATCCCCAGTACACCCGCCCGGCCGACTTTCG
AGGCTGGCAGGTGCCGGAGGTGTTACTCAGCGGTCATCACGCCCAGATCGCCCACTGGCGGCGCGAGCAGCAACTGACGC
GCACTCGGGAGCGGCGGCCGGACTTGCTGCCGCCGGAAGCGTGA

Upstream 100 bases:

>100_bases
CTCGGCAAGCCAACTGGAAAGCAGCGGATCGAGCAGCAGTGGATGCGGCAGAGCGTAGCGATTGGTGAGCATAATCTGAT
CGTAGGCCCCGTCAGGTGCA

Downstream 100 bases:

>100_bases
CCCATGCAATATCTGTGGTTGGTGGTCTTTGTGCTGTGTACAGCCTGGGCGGCTGGGGCTGAGGAACGGCTGGTAGTAGG
CTCCAAGCGGTTTACCGAGT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MRIDIVTLFPEFFVSPLQCSLLARAIAGGVCNIAITNPRDFAADRYRTVDDTPYGGGAGMVLKPEPLFAAVESLPIIAPR
AVILLTPQGRPLKQVFLRSLAADYAQLVLLCGHYEGVDERVRAHLATHEISLGDFVLTGGEIPALALIDGIVRLLPGTVG
NRASLESESFEDNLLEYPQYTRPADFRGWQVPEVLLSGHHAQIAHWRREQQLTRTRERRPDLLPPEA

Sequences:

>Translated_227_residues
MRIDIVTLFPEFFVSPLQCSLLARAIAGGVCNIAITNPRDFAADRYRTVDDTPYGGGAGMVLKPEPLFAAVESLPIIAPR
AVILLTPQGRPLKQVFLRSLAADYAQLVLLCGHYEGVDERVRAHLATHEISLGDFVLTGGEIPALALIDGIVRLLPGTVG
NRASLESESFEDNLLEYPQYTRPADFRGWQVPEVLLSGHHAQIAHWRREQQLTRTRERRPDLLPPEA
>Mature_227_residues
MRIDIVTLFPEFFVSPLQCSLLARAIAGGVCNIAITNPRDFAADRYRTVDDTPYGGGAGMVLKPEPLFAAVESLPIIAPR
AVILLTPQGRPLKQVFLRSLAADYAQLVLLCGHYEGVDERVRAHLATHEISLGDFVLTGGEIPALALIDGIVRLLPGTVG
NRASLESESFEDNLLEYPQYTRPADFRGWQVPEVLLSGHHAQIAHWRREQQLTRTRERRPDLLPPEA

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=225, Percent_Identity=46.6666666666667, Blast_Score=201, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_GLOVI (Q7MBC8)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_923835.1
- ProteinModelPortal:   Q7MBC8
- SMR:   Q7MBC8
- GeneID:   2599208
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:glr0889
- NMPDR:   fig|251221.1.peg.889
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BioCyc:   GVIO251221:GLR0889-MONOMER
- BRENDA:   2.1.1.31
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 25097; Mature: 25097

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDIVTLFPEFFVSPLQCSLLARAIAGGVCNIAITNPRDFAADRYRTVDDTPYGGGAGM
CEEEEEEECHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHCCCCCCCCCCCCCCE
VLKPEPLFAAVESLPIIAPRAVILLTPQGRPLKQVFLRSLAADYAQLVLLCGHYEGVDER
EECCCHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VRAHLATHEISLGDFVLTGGEIPALALIDGIVRLLPGTVGNRASLESESFEDNLLEYPQY
HHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCC
TRPADFRGWQVPEVLLSGHHAQIAHWRREQQLTRTRERRPDLLPPEA
CCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRIDIVTLFPEFFVSPLQCSLLARAIAGGVCNIAITNPRDFAADRYRTVDDTPYGGGAGM
CEEEEEEECHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHCCCCCCCCCCCCCCE
VLKPEPLFAAVESLPIIAPRAVILLTPQGRPLKQVFLRSLAADYAQLVLLCGHYEGVDER
EECCCHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VRAHLATHEISLGDFVLTGGEIPALALIDGIVRLLPGTVGNRASLESESFEDNLLEYPQY
HHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCC
TRPADFRGWQVPEVLLSGHHAQIAHWRREQQLTRTRERRPDLLPPEA
CCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292