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The map label for this gene is gidB

Identifier: 37520277

GI number: 37520277

Start: 752953

End: 753708

Strand: Direct

Name: gidB

Synonym: gvip073

Alternate gene names: 37520277

Gene position: 752953-753708 (Clockwise)

Preceding gene: 37520276

Following gene: 37520279

Centisome position: 16.16

GC content: 66.4

Gene sequence:

>756_bases
ATGGACTCCGGGGCGCTCTGGCAGGATCTGGGCTGGCAGCCGGATACGCTCCAAGCCCGCAGCTTCGAGCGGCTCTACGC
ACTGGTGCTCGCGGGCAACACCCGGCTCAACCTCACCCGCATCACAGGGCGCGAGGAATTCTGGGAGAAGCACCTGTTCG
ACTCCCTGCGCGGGCTCGCCGCATTTCAAGATCAAAAAGAACCGAGCCTCATCGACATCGGCACCGGCGCCGGCTTTCCA
GGGCTGCCTATCGCGATCGCCCACCCCGACTGGTACGTGGTGCTCGTCGATTCGGTGCGCAAAAAAATTGCCTTTGTGCT
CTCAACCATCCAGGCTCTGGGACTCACCAACGCCCAGGCCCTCACCGGTCGGGCCGAGGATCTGGCCCATCGGCGCGAGC
ACCGCGAAAGTTACGATCTGGCGGTGCTCAGGGCCGTGGCCCAGGCCAACGTTTGTGCGGAGTACGCTCTGCCGTTTGTC
AAGCTGGGCGGTGCGGCGGTGCTTTACCGGGGCAACTGGGAGGTGCAGGAGGAGGTGGAACTGGCCCGGGCCTGCCGGGC
GCTGGGCGGGGAGATTGTCGAAGTGGACGCCTTCGAGCTGCCGGTGAGCCGGGCGGTGCGCCACTGCGTCGTGATTCGCA
AAACCGGCCCGGGGCTCAGGGTATTCCCGCGCCCGGCGGGCTTGCCCACACAACATCCCCTCGGAGCCATCGAAGGGGCC
CCGAGGGTCGAATCGGAGGAGCCGGAGGAGCCTTAG

Upstream 100 bases:

>100_bases
CATCGCCTCCGACACGGTCAAGGGAGCAGGTTGCACCCCCCAGACTGCCCAGCGCGGCTTCCAGATAAAAACCGCCGACG
CGGGAACCAAGTAATACCCC

Downstream 100 bases:

>100_bases
TACTTGCGCACCAGGCCGACGACTACCCCCAACACCTCCAGGCCGCTGGCCGGGTGGATGGGGGAGTAGCGGCTGTTGGC
GGGCTCCAGCCAGGTCTGCT

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MDSGALWQDLGWQPDTLQARSFERLYALVLAGNTRLNLTRITGREEFWEKHLFDSLRGLAAFQDQKEPSLIDIGTGAGFP
GLPIAIAHPDWYVVLVDSVRKKIAFVLSTIQALGLTNAQALTGRAEDLAHRREHRESYDLAVLRAVAQANVCAEYALPFV
KLGGAAVLYRGNWEVQEEVELARACRALGGEIVEVDAFELPVSRAVRHCVVIRKTGPGLRVFPRPAGLPTQHPLGAIEGA
PRVESEEPEEP

Sequences:

>Translated_251_residues
MDSGALWQDLGWQPDTLQARSFERLYALVLAGNTRLNLTRITGREEFWEKHLFDSLRGLAAFQDQKEPSLIDIGTGAGFP
GLPIAIAHPDWYVVLVDSVRKKIAFVLSTIQALGLTNAQALTGRAEDLAHRREHRESYDLAVLRAVAQANVCAEYALPFV
KLGGAAVLYRGNWEVQEEVELARACRALGGEIVEVDAFELPVSRAVRHCVVIRKTGPGLRVFPRPAGLPTQHPLGAIEGA
PRVESEEPEEP
>Mature_251_residues
MDSGALWQDLGWQPDTLQARSFERLYALVLAGNTRLNLTRITGREEFWEKHLFDSLRGLAAFQDQKEPSLIDIGTGAGFP
GLPIAIAHPDWYVVLVDSVRKKIAFVLSTIQALGLTNAQALTGRAEDLAHRREHRESYDLAVLRAVAQANVCAEYALPFV
KLGGAAVLYRGNWEVQEEVELARACRALGGEIVEVDAFELPVSRAVRHCVVIRKTGPGLRVFPRPAGLPTQHPLGAIEGA
PRVESEEPEEP

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=118, Percent_Identity=29.6610169491525, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_GLOVI (Q7NMQ7)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_923654.1
- ProteinModelPortal:   Q7NMQ7
- SMR:   Q7NMQ7
- GeneID:   2599427
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.708
- HOGENOM:   HBG686577
- OMA:   HLWDSLR
- ProtClustDB:   PRK00107
- BioCyc:   GVIO251221:GLR0708-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.-

Molecular weight: Translated: 27622; Mature: 27622

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSGALWQDLGWQPDTLQARSFERLYALVLAGNTRLNLTRITGREEFWEKHLFDSLRGLA
CCCCCCHHHCCCCCCCCHHHHHHHHHHHEEECCCEEEEEEECCHHHHHHHHHHHHHHHHH
AFQDQKEPSLIDIGTGAGFPGLPIAIAHPDWYVVLVDSVRKKIAFVLSTIQALGLTNAQA
HHCCCCCCCEEEECCCCCCCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHHCCCCHHH
LTGRAEDLAHRREHRESYDLAVLRAVAQANVCAEYALPFVKLGGAAVLYRGNWEVQEEVE
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEECCEEEEEECCCCHHHHHH
LARACRALGGEIVEVDAFELPVSRAVRHCVVIRKTGPGLRVFPRPAGLPTQHPLGAIEGA
HHHHHHHHCCCEEEECHHHCCHHHHHHHEEEEEECCCCCEEECCCCCCCCCCCCCCCCCC
PRVESEEPEEP
CCCCCCCCCCC
>Mature Secondary Structure
MDSGALWQDLGWQPDTLQARSFERLYALVLAGNTRLNLTRITGREEFWEKHLFDSLRGLA
CCCCCCHHHCCCCCCCCHHHHHHHHHHHEEECCCEEEEEEECCHHHHHHHHHHHHHHHHH
AFQDQKEPSLIDIGTGAGFPGLPIAIAHPDWYVVLVDSVRKKIAFVLSTIQALGLTNAQA
HHCCCCCCCEEEECCCCCCCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHHCCCCHHH
LTGRAEDLAHRREHRESYDLAVLRAVAQANVCAEYALPFVKLGGAAVLYRGNWEVQEEVE
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEECCEEEEEECCCCHHHHHH
LARACRALGGEIVEVDAFELPVSRAVRHCVVIRKTGPGLRVFPRPAGLPTQHPLGAIEGA
HHHHHHHHCCCEEEECHHHCCHHHHHHHEEEEEECCCCCEEECCCCCCCCCCCCCCCCCC
PRVESEEPEEP
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292