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The map label for this gene is thiE [H]

Identifier: 37519972

GI number: 37519972

Start: 423314

End: 424414

Strand: Reverse

Name: thiE [H]

Synonym: gvip037

Alternate gene names: 37519972

Gene position: 424414-423314 (Counterclockwise)

Preceding gene: 37519974

Following gene: 37519971

Centisome position: 9.11

GC content: 67.03

Gene sequence:

>1101_bases
ATGGCGCCCCCCGCAATGCGGTGGCAGAGAGTCGTTTTTGGTGGGCCTGGGCAGGGGAGCATCGGCGGTGCTTCAATGGC
GGTTGTGGAAGAGCAAGTGGTTCTGAGAATTCTCGATGCCAACCTCGATCGCGCCCGCGAAGGCGTGCGGGTCGTCGAAG
AATGGTTGCGCTTCGGCGGCGGTCCGGGCGAGTCCCTCGCCGAGTGCAAAGCGTTGCGCCAGCAACTGGGCCGCTTTCAC
ACCGAGCGGCTGCGCGCCGCCCGCGACACGCCCCACGACCCGGGCACCGGGCTGGAGCACCCCGACGAAGGGGTGCGTAG
CAGCCCTCTGGATGTGGTAAGGGTCAATTTTGCCCGCATTCAAGAAGCCCTGCGCGTCCTAGAAGAGTACGCCAAGCTCG
TCCAGCCGGATCTGGCCGCCGCGGCCAAGGAATGGCGCTACCGAGTCTATACCCTCGAAAGTACCGCCACCGGCGGCGAT
CTGCGCACCCGGCTTGCGGCGGCCCGGCTCTACCTGGTCACCTCGCCGCACCCCGAACTGATCGAGATCGTGGAGCACGC
CCTGGCTGTGGGGTTGCCGCTGGTGCAATTGCGCGAAAAAGAAGCGCCCGCCCGCGCCGTACTCGATATCGCTCTAAGGT
TGCGGGATGTCACCCTTCGCCACGGAGCGCTGTTTATAGTCAACGACCGGGTGGATCTGGCCCTCGCCTGTGGGGCGGAC
GGGGTGCACCTCGGCCAGGAGGATCTGCCGCTCGCTCGGGCGCGGGCTTTGATGGGACCGCGTTTGCTCATTGGCCAATC
GACCCACGCCCCGGCCGAGGCGCAACAAGCCGTCGCAGACGGGGCCGATTACCTGGGCGTCGGCCCCGTCTACGCCACTC
CCACCAAACAGGGGCGCACGCCCGTGGGCCTGGAGTACGTGCGCCACTGCCGCGAATCGATCGAACGACCGGGCTTCGCC
ATCGGCGGCATCGACCGGAGCAATCTGGAGGCGGTGATCGCCGCCGGAGCCGAGCGCATCGCGGTGGTGCGTGCGATCAT
GGCAGCGGAAGATCCGGGCCGCACGACCGCCTGGTTTCTGGAGAGGTTGAACCGTGGTTGA

Upstream 100 bases:

>100_bases
ATAAAAGCTTATCGGCGACACCACATTAACATGGCAAAACCCTGAATTGCGGGGATGATCCACAACATTTAGCGCTTTCT
CCCCCGCGCAAAGCAACAGA

Downstream 100 bases:

>100_bases
AACGGTGCGCGTCAACGGCGAGGAGCGGACGTTCGCCCCTGGCACGACGGTGAGTGCGATGCTCGCCCAACTGGCCCTCG
AACCGCGGCTGCTGGTCATC

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MAPPAMRWQRVVFGGPGQGSIGGASMAVVEEQVVLRILDANLDRAREGVRVVEEWLRFGGGPGESLAECKALRQQLGRFH
TERLRAARDTPHDPGTGLEHPDEGVRSSPLDVVRVNFARIQEALRVLEEYAKLVQPDLAAAAKEWRYRVYTLESTATGGD
LRTRLAAARLYLVTSPHPELIEIVEHALAVGLPLVQLREKEAPARAVLDIALRLRDVTLRHGALFIVNDRVDLALACGAD
GVHLGQEDLPLARARALMGPRLLIGQSTHAPAEAQQAVADGADYLGVGPVYATPTKQGRTPVGLEYVRHCRESIERPGFA
IGGIDRSNLEAVIAAGAERIAVVRAIMAAEDPGRTTAWFLERLNRG

Sequences:

>Translated_366_residues
MAPPAMRWQRVVFGGPGQGSIGGASMAVVEEQVVLRILDANLDRAREGVRVVEEWLRFGGGPGESLAECKALRQQLGRFH
TERLRAARDTPHDPGTGLEHPDEGVRSSPLDVVRVNFARIQEALRVLEEYAKLVQPDLAAAAKEWRYRVYTLESTATGGD
LRTRLAAARLYLVTSPHPELIEIVEHALAVGLPLVQLREKEAPARAVLDIALRLRDVTLRHGALFIVNDRVDLALACGAD
GVHLGQEDLPLARARALMGPRLLIGQSTHAPAEAQQAVADGADYLGVGPVYATPTKQGRTPVGLEYVRHCRESIERPGFA
IGGIDRSNLEAVIAAGAERIAVVRAIMAAEDPGRTTAWFLERLNRG
>Mature_365_residues
APPAMRWQRVVFGGPGQGSIGGASMAVVEEQVVLRILDANLDRAREGVRVVEEWLRFGGGPGESLAECKALRQQLGRFHT
ERLRAARDTPHDPGTGLEHPDEGVRSSPLDVVRVNFARIQEALRVLEEYAKLVQPDLAAAAKEWRYRVYTLESTATGGDL
RTRLAAARLYLVTSPHPELIEIVEHALAVGLPLVQLREKEAPARAVLDIALRLRDVTLRHGALFIVNDRVDLALACGADG
VHLGQEDLPLARARALMGPRLLIGQSTHAPAEAQQAVADGADYLGVGPVYATPTKQGRTPVGLEYVRHCRESIERPGFAI
GGIDRSNLEAVIAAGAERIAVVRAIMAAEDPGRTTAWFLERLNRG

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=193, Percent_Identity=38.3419689119171, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6325042, Length=207, Percent_Identity=33.8164251207729, Blast_Score=106, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR016229
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 39733; Mature: 39602

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPPAMRWQRVVFGGPGQGSIGGASMAVVEEQVVLRILDANLDRAREGVRVVEEWLRFGG
CCCCCCHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
GPGESLAECKALRQQLGRFHTERLRAARDTPHDPGTGLEHPDEGVRSSPLDVVRVNFARI
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
QEALRVLEEYAKLVQPDLAAAAKEWRYRVYTLESTATGGDLRTRLAAARLYLVTSPHPEL
HHHHHHHHHHHHHHCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHEEEEECCCHHH
IEIVEHALAVGLPLVQLREKEAPARAVLDIALRLRDVTLRHGALFIVNDRVDLALACGAD
HHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHEECCEEEEEECCCCEEEEECCC
GVHLGQEDLPLARARALMGPRLLIGQSTHAPAEAQQAVADGADYLGVGPVYATPTKQGRT
CCCCCCCCCCHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCEEECCCCCCCC
PVGLEYVRHCRESIERPGFAIGGIDRSNLEAVIAAGAERIAVVRAIMAAEDPGRTTAWFL
CCCHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHH
ERLNRG
HHHCCC
>Mature Secondary Structure 
APPAMRWQRVVFGGPGQGSIGGASMAVVEEQVVLRILDANLDRAREGVRVVEEWLRFGG
CCCCCHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
GPGESLAECKALRQQLGRFHTERLRAARDTPHDPGTGLEHPDEGVRSSPLDVVRVNFARI
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
QEALRVLEEYAKLVQPDLAAAAKEWRYRVYTLESTATGGDLRTRLAAARLYLVTSPHPEL
HHHHHHHHHHHHHHCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHEEEEECCCHHH
IEIVEHALAVGLPLVQLREKEAPARAVLDIALRLRDVTLRHGALFIVNDRVDLALACGAD
HHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHEECCEEEEEECCCCEEEEECCC
GVHLGQEDLPLARARALMGPRLLIGQSTHAPAEAQQAVADGADYLGVGPVYATPTKQGRT
CCCCCCCCCCHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCEEECCCCCCCC
PVGLEYVRHCRESIERPGFAIGGIDRSNLEAVIAAGAERIAVVRAIMAAEDPGRTTAWFL
CCCHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHH
ERLNRG
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292 [H]