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The map label for this gene is rpoD [H]

Identifier: 37519772

GI number: 37519772

Start: 194739

End: 195809

Strand: Reverse

Name: rpoD [H]

Synonym: gll0203

Alternate gene names: 37519772

Gene position: 195809-194739 (Counterclockwise)

Preceding gene: 37519774

Following gene: 37519771

Centisome position: 4.2

GC content: 61.72

Gene sequence:

>1071_bases
ATGAGTTTGGGTGGCGCAAGCGCCCGCGGCAATCGCAAAAGTACTCGTTTTGTGATGAATATTGCTTGCATTGGGTTACG
AAAAGCGAAGAACTGTGGACTATTCGCGAGGGGCTCCCCCATGATGAGTGCACGGGGAACGCTATTCAATCGGCATGGGG
TACCGACCGTGGCAGAAACAGCACAGGGCAAGCCGGCAGAGGCCCGCAAAACTCGGCAAAGCGCTTCATTTGAGAGAGAC
TCGGTAGGGCTTTACCTGCAAGAGATCGGCAGGATCCCCCTGCTCAAGCCCGAAGAAGAGGTGATGCTCGCCCGGGCGAT
TGCCCAGGGCGGCCCCAAGGGCGAGTGGGCCAAGCGCAAGCTGGTGCAGGCCAACCTGCGCCTGGTCGTGTCGATTGCCA
AAAAGTATCTCAACCGCGGCGTGCCGTTCCTGGATCTGATCCAGGAAGGCTCGATGGGCCTGATTCGCGCCGCCGAAAAA
TTCGAGCACGAGCGCGGTTACAAGTTCTCGACCTACGCCTACTGGTGGATCCGCCAGGCGATTACCCGGGCGGTCGCTTC
CCAGGCGCGCACCGTGCGTCTGCCGGTGCATCTGGTCGAAAAAATCAACCGCGTCAAAAAGGTCCGTCGCACCCTCGCCC
AGGAATTGGGCCGCACGCCGAGCCAGGGGGAACTGGCCGAGGCGCTGGAATTGGCCGAAGAGGATCTTGAGCACATCCTG
CGCGCCGCCCGGCGCACCGTGTCCCTCAACATCACCGTCGGCAAAGAGGAAGACACCGAGCTGATTCATCTCATTGAAGA
CAGCCAGACCGACCAGCCCGACGTGCACATCGACCGCGAGTCGATGCGCGTCGAGGTGAGCGATCTATTGGATCGGCATC
TCACGCCCCGCGAGCGCGACATTTTGCAGTTGCGCTTCGGGCTGCGCGACGGCCAGCAGCGCACCCTCGATCAGGTGGGC
AGAATGTTCGACCTTTCGCGCGAGCGCATCCGCCAGATCCAGGCCAAAGCCTTCCGCAAACTGCGCCGGGTCCGCCAGCG
CCAGCGCCTGCAGGACTGGATCGGGATTTAG

Upstream 100 bases:

>100_bases
CAGTACCTGGAGGGCCGCCCAGCCCGCAGCCACCAATAAAAGCCAGGTGAGCAACGTGCGTTGCAGGTTCGTCAGCATCG
GCATTGCAACCTAGTGTTAC

Downstream 100 bases:

>100_bases
AACAGGTTCCGGACATCCGGTGCCAAATTTCTTAGGCGGCTTGGAAATCTTTCCAGGCCGCCTGCTGCTATCTGTGTACC
AAGATAGGGGCTGCCGTTCT

Product: group 2 sigma 70-type sigma factor

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MSLGGASARGNRKSTRFVMNIACIGLRKAKNCGLFARGSPMMSARGTLFNRHGVPTVAETAQGKPAEARKTRQSASFERD
SVGLYLQEIGRIPLLKPEEEVMLARAIAQGGPKGEWAKRKLVQANLRLVVSIAKKYLNRGVPFLDLIQEGSMGLIRAAEK
FEHERGYKFSTYAYWWIRQAITRAVASQARTVRLPVHLVEKINRVKKVRRTLAQELGRTPSQGELAEALELAEEDLEHIL
RAARRTVSLNITVGKEEDTELIHLIEDSQTDQPDVHIDRESMRVEVSDLLDRHLTPRERDILQLRFGLRDGQQRTLDQVG
RMFDLSRERIRQIQAKAFRKLRRVRQRQRLQDWIGI

Sequences:

>Translated_356_residues
MSLGGASARGNRKSTRFVMNIACIGLRKAKNCGLFARGSPMMSARGTLFNRHGVPTVAETAQGKPAEARKTRQSASFERD
SVGLYLQEIGRIPLLKPEEEVMLARAIAQGGPKGEWAKRKLVQANLRLVVSIAKKYLNRGVPFLDLIQEGSMGLIRAAEK
FEHERGYKFSTYAYWWIRQAITRAVASQARTVRLPVHLVEKINRVKKVRRTLAQELGRTPSQGELAEALELAEEDLEHIL
RAARRTVSLNITVGKEEDTELIHLIEDSQTDQPDVHIDRESMRVEVSDLLDRHLTPRERDILQLRFGLRDGQQRTLDQVG
RMFDLSRERIRQIQAKAFRKLRRVRQRQRLQDWIGI
>Mature_355_residues
SLGGASARGNRKSTRFVMNIACIGLRKAKNCGLFARGSPMMSARGTLFNRHGVPTVAETAQGKPAEARKTRQSASFERDS
VGLYLQEIGRIPLLKPEEEVMLARAIAQGGPKGEWAKRKLVQANLRLVVSIAKKYLNRGVPFLDLIQEGSMGLIRAAEKF
EHERGYKFSTYAYWWIRQAITRAVASQARTVRLPVHLVEKINRVKKVRRTLAQELGRTPSQGELAEALELAEEDLEHILR
AARRTVSLNITVGKEEDTELIHLIEDSQTDQPDVHIDRESMRVEVSDLLDRHLTPRERDILQLRFGLRDGQQRTLDQVGR
MFDLSRERIRQIQAKAFRKLRRVRQRQRLQDWIGI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=252, Percent_Identity=48.015873015873, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1789098, Length=272, Percent_Identity=42.2794117647059, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1789871, Length=274, Percent_Identity=25.9124087591241, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788231, Length=207, Percent_Identity=26.5700483091787, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 40644; Mature: 40512

Theoretical pI: Translated: 11.10; Mature: 11.10

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLGGASARGNRKSTRFVMNIACIGLRKAKNCGLFARGSPMMSARGTLFNRHGVPTVAET
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHCCCHHCCCCCCCHHHH
AQGKPAEARKTRQSASFERDSVGLYLQEIGRIPLLKPEEEVMLARAIAQGGPKGEWAKRK
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHH
LVQANLRLVVSIAKKYLNRGVPFLDLIQEGSMGLIRAAEKFEHERGYKFSTYAYWWIRQA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ITRAVASQARTVRLPVHLVEKINRVKKVRRTLAQELGRTPSQGELAEALELAEEDLEHIL
HHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
RAARRTVSLNITVGKEEDTELIHLIEDSQTDQPDVHIDRESMRVEVSDLLDRHLTPRERD
HHHHHEEEEEEEECCCCCCHHEEEECCCCCCCCCEEECHHHHHEEHHHHHHHCCCCCHHH
ILQLRFGLRDGQQRTLDQVGRMFDLSRERIRQIQAKAFRKLRRVRQRQRLQDWIGI
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLGGASARGNRKSTRFVMNIACIGLRKAKNCGLFARGSPMMSARGTLFNRHGVPTVAET
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHCCCHHCCCCCCCHHHH
AQGKPAEARKTRQSASFERDSVGLYLQEIGRIPLLKPEEEVMLARAIAQGGPKGEWAKRK
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHH
LVQANLRLVVSIAKKYLNRGVPFLDLIQEGSMGLIRAAEKFEHERGYKFSTYAYWWIRQA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ITRAVASQARTVRLPVHLVEKINRVKKVRRTLAQELGRTPSQGELAEALELAEEDLEHIL
HHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
RAARRTVSLNITVGKEEDTELIHLIEDSQTDQPDVHIDRESMRVEVSDLLDRHLTPRERD
HHHHHEEEEEEEECCCCCCHHEEEECCCCCCCCCEEECHHHHHEEHHHHHHHCCCCCHHH
ILQLRFGLRDGQQRTLDQVGRMFDLSRERIRQIQAKAFRKLRRVRQRQRLQDWIGI
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1901566; 11759840 [H]