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The map label for this gene is proB

Identifier: 37519638

GI number: 37519638

Start: 70061

End: 71170

Strand: Reverse

Name: proB

Synonym: gvip004

Alternate gene names: 37519638

Gene position: 71170-70061 (Counterclockwise)

Preceding gene: 37519639

Following gene: 37519637

Centisome position: 1.53

GC content: 68.47

Gene sequence:

>1110_bases
ATGGTCGCGCTGGTGGTCAAAATCGGCACATCGAGCCTGAGCGACCCGAGCACAGGGGATTTGCGCCTGGCGACGCTTGG
GGGGCTCGCCGAGACGCTCACGCGGCTGCGGCGCGCGGGCCACCGGATCATCCTGGTCAGCAGTGGGGCGGTGGGGGTCG
GCTGCGCACGGCTCGGCCTCAAGGAGCGCCCCGCCACCGTGGCGGGCAAGCAGGCCGCCGCCGCCGTCGGGCAGGGGCTG
CTGATGAGCATGTACGATCGCCTCTTCGGAGCGCTCGGCCAACCGGTGGCCCAGGTATTACTCACCCGCCAGGATTTGAT
GGACCGCGTCCGCTACCTCAACGCCCGCGAAACGTTGAGCGAATTGTGGCGACTCGGGACGGTGCCCATCGTCAACGAAA
ACGACACGGTGGCCACCGACGAGTTGCGCTTCGGGGACAACGACGCCCTCTCGGCGCTGGTGGCGGGGCTGGTCGAGGCG
CAGTGGCTGGTGCTGCTCACCGACGTGGCCGGGCTCTACTCGGCCAACCCGCGCCTCGATCCGCAGGCACGGCTTTTGTC
CGAGGTGACCGAGATTTCTGAAGAACTGCTGCAGTCGGCCCGCGGGCGCAGCCTCTGGGGCAGCGGCGGCATGGCGAGCA
AACTGGAGGCGGCGCGCATCGCCGCGAGCGCCGGGGTGGCCACCGTGATCACCGAGGGAAACACCCCGCAAAATATCGAG
CGCATCCTGGCGGGCGAAGCAATCGGCACCCGATTTGCCCTGGCGCGGCCCGGGGGGCGTCTGTCGCTGCGCAAGCGCTG
GATCGGCTACGGTCTGGTGCCCGCCGGGGCGCTCCACCTCGACGAGGGGGCGGTCCTGGCGGTGCGCGAAGGGGGCAAAT
CGCTGCTGCCGGCCGGGGTGAGGGGCGTGGAAGGCCGCTTCGAGACGGGAGCGCTGGTGCGGCTCATCGACGGGCAGGGC
CTCGAATTTGCCCGGGGGCTCGTGAATTATTCCAGCGAAGAACTGGAAAAAATCCGCGGCCGCAAAAGCCATGAGATTGC
CGCGCTTTTGGGTATCGAAGGACAACCGCCCACGGCCGTGCACCGCGACAATCTGGTCACCCTTTCTTGA

Upstream 100 bases:

>100_bases
CCCTCAAGACCAGCCAGGTGCGCCTGTCGGTCGAAGTGGACCTGAACCAGTTGCGCGAGGATATTCAAGTATTTTTGCAG
GCGGTGTAGAGGCAGGCGGC

Downstream 100 bases:

>100_bases
AAAAGCGCTATATTTAATGCCTGCTTCCAAAACGATGGGGATTGGCCCCGTTTCTATCCACTTGTAGGCACATGCATAAG
ATGATGTCCTTTCAGCCCCA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MVALVVKIGTSSLSDPSTGDLRLATLGGLAETLTRLRRAGHRIILVSSGAVGVGCARLGLKERPATVAGKQAAAAVGQGL
LMSMYDRLFGALGQPVAQVLLTRQDLMDRVRYLNARETLSELWRLGTVPIVNENDTVATDELRFGDNDALSALVAGLVEA
QWLVLLTDVAGLYSANPRLDPQARLLSEVTEISEELLQSARGRSLWGSGGMASKLEAARIAASAGVATVITEGNTPQNIE
RILAGEAIGTRFALARPGGRLSLRKRWIGYGLVPAGALHLDEGAVLAVREGGKSLLPAGVRGVEGRFETGALVRLIDGQG
LEFARGLVNYSSEELEKIRGRKSHEIAALLGIEGQPPTAVHRDNLVTLS

Sequences:

>Translated_369_residues
MVALVVKIGTSSLSDPSTGDLRLATLGGLAETLTRLRRAGHRIILVSSGAVGVGCARLGLKERPATVAGKQAAAAVGQGL
LMSMYDRLFGALGQPVAQVLLTRQDLMDRVRYLNARETLSELWRLGTVPIVNENDTVATDELRFGDNDALSALVAGLVEA
QWLVLLTDVAGLYSANPRLDPQARLLSEVTEISEELLQSARGRSLWGSGGMASKLEAARIAASAGVATVITEGNTPQNIE
RILAGEAIGTRFALARPGGRLSLRKRWIGYGLVPAGALHLDEGAVLAVREGGKSLLPAGVRGVEGRFETGALVRLIDGQG
LEFARGLVNYSSEELEKIRGRKSHEIAALLGIEGQPPTAVHRDNLVTLS
>Mature_369_residues
MVALVVKIGTSSLSDPSTGDLRLATLGGLAETLTRLRRAGHRIILVSSGAVGVGCARLGLKERPATVAGKQAAAAVGQGL
LMSMYDRLFGALGQPVAQVLLTRQDLMDRVRYLNARETLSELWRLGTVPIVNENDTVATDELRFGDNDALSALVAGLVEA
QWLVLLTDVAGLYSANPRLDPQARLLSEVTEISEELLQSARGRSLWGSGGMASKLEAARIAASAGVATVITEGNTPQNIE
RILAGEAIGTRFALARPGGRLSLRKRWIGYGLVPAGALHLDEGAVLAVREGGKSLLPAGVRGVEGRFETGALVRLIDGQG
LEFARGLVNYSSEELEKIRGRKSHEIAALLGIEGQPPTAVHRDNLVTLS

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=290, Percent_Identity=31.0344827586207, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI62912457, Length=288, Percent_Identity=31.25, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1786437, Length=367, Percent_Identity=46.5940054495913, Blast_Score=289, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17569969, Length=265, Percent_Identity=36.2264150943396, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17569967, Length=265, Percent_Identity=36.2264150943396, Blast_Score=142, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6320506, Length=414, Percent_Identity=38.1642512077295, Blast_Score=257, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6321822, Length=413, Percent_Identity=36.319612590799, Blast_Score=234, Evalue=2e-62,
Organism=Drosophila melanogaster, GI21357643, Length=283, Percent_Identity=34.2756183745583, Blast_Score=135, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_GLOVI (Q7NPI6)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_923015.1
- ProteinModelPortal:   Q7NPI6
- GeneID:   2600046
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.69
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- BioCyc:   GVIO251221:GLL0069-MONOMER
- BRENDA:   2.7.2.11
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 38985; Mature: 38985

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVALVVKIGTSSLSDPSTGDLRLATLGGLAETLTRLRRAGHRIILVSSGAVGVGCARLGL
CEEEEEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCC
KERPATVAGKQAAAAVGQGLLMSMYDRLFGALGQPVAQVLLTRQDLMDRVRYLNARETLS
CCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHH
ELWRLGTVPIVNENDTVATDELRFGDNDALSALVAGLVEAQWLVLLTDVAGLYSANPRLD
HHHHCCCCEEECCCCCEEECCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PQARLLSEVTEISEELLQSARGRSLWGSGGMASKLEAARIAASAGVATVITEGNTPQNIE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCHHHHH
RILAGEAIGTRFALARPGGRLSLRKRWIGYGLVPAGALHLDEGAVLAVREGGKSLLPAGV
HHHHCHHHCCEEEEECCCCCHHHHHHHHCCCCCCCCEEEECCCCEEEEECCCCCCCCCCC
RGVEGRFETGALVRLIDGQGLEFARGLVNYSSEELEKIRGRKSHEIAALLGIEGQPPTAV
CCCCCCCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCC
HRDNLVTLS
CCCCEEEEC
>Mature Secondary Structure
MVALVVKIGTSSLSDPSTGDLRLATLGGLAETLTRLRRAGHRIILVSSGAVGVGCARLGL
CEEEEEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCC
KERPATVAGKQAAAAVGQGLLMSMYDRLFGALGQPVAQVLLTRQDLMDRVRYLNARETLS
CCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHH
ELWRLGTVPIVNENDTVATDELRFGDNDALSALVAGLVEAQWLVLLTDVAGLYSANPRLD
HHHHCCCCEEECCCCCEEECCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PQARLLSEVTEISEELLQSARGRSLWGSGGMASKLEAARIAASAGVATVITEGNTPQNIE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCHHHHH
RILAGEAIGTRFALARPGGRLSLRKRWIGYGLVPAGALHLDEGAVLAVREGGKSLLPAGV
HHHHCHHHCCEEEEECCCCCHHHHHHHHCCCCCCCCEEEECCCCEEEEECCCCCCCCCCC
RGVEGRFETGALVRLIDGQGLEFARGLVNYSSEELEKIRGRKSHEIAALLGIEGQPPTAV
CCCCCCCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCC
HRDNLVTLS
CCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292