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The map label for this gene is priA [H]

Identifier: 37519594

GI number: 37519594

Start: 23846

End: 26275

Strand: Reverse

Name: priA [H]

Synonym: gll0025

Alternate gene names: 37519594

Gene position: 26275-23846 (Counterclockwise)

Preceding gene: 37519595

Following gene: 37519592

Centisome position: 0.56

GC content: 67.82

Gene sequence:

>2430_bases
ATGGAAACTTTCCGGCCGGGTCCCCTGCTGCGGCCCGCGGCCTGGGTGCAGGTGCTCGTCGACGCACCGGCCCGGGAGCG
CACCTACACCTACCGACTGGCCGACGGCATGACCGCCGCCGGGGGCGATGTGGTGTGCGTGCCCTTCGGCAGCCAGTTGG
TTGGAGGTATCGTCCTCGGTTGTCTCGACCAATTGCCGACGGGCCTCGATCCTGGGCGGCTCAAAACCGTCGAGTCGGTC
GTGGGGACGGGGCTGTTTGCGCCAGGCTTCTGGCCGCTGCTGGTGCGGGTGGCCGACTATTATCTGGTGCCGCTGGCGCG
GGTGCTCGAGACGGCTTTGCCGCCGGGCATCTTGAGCCGTGCCCGGCGGCGGGTGCGTCTTGTGGCCGATGCCGCTCCGA
TGGCCCAGTGGGGGCTCAGTGCGGCAGCCAAAAGCGCGTTCGATTTTTTGCGCTCTCAGGCCCACGCGGATTTCAGCTGG
CGCTTTTGTGTGCAGCGGCTGAGTGGAGGAGTGTCCTCCCTGAGAGAACTGCAGGCGCGCCGGTTACTTGAGAGTTACTA
CGTCTTCGGTGAGACGGCCCGGCCGCGCACGCAGCAATTTGTCGTGCTCGCAGGAGCCGGTGCCGATCTCTGCGGCCGTT
CGGCGGCGGTGGTGCAGTGTTTGCGTCGGCTCGGGGGAGAGGTGAGCGTCGAGCAACTGCTCGCCGAAGCGCGCACCACC
CGCGCGCTATTGCAGAAGTTGGACGCCGGGGGCCACGTGCGCATCTACGAGCGCCAGATTTTGCGCCTTGCCGCTCCGAC
GGCGTCCGCCGATGCGCCAAAGCTGCTCACCGACGCCCAGAAGCAGGTGCTGGACCGCCTGCAGGGGGCGACTCCCGGTC
CGGTGCTGCTCGAAGGGGTGACCGGTTCCGGCAAAACCGAGGTCTACTTGCAGGCGATTGCCCCGGTGCTGGACCGCGGC
GAGTCGGCTCTGGTGCTGGTGCCTGAAATTGGCCTCACCCCGCAGTTGACCGACCGCTTCGCCGCCCGCTTCGGGGCAAG
GGTGCGTGTCTACCACTCGGCCCTTTCGGAGGGCGAGCGCTTCGACTGCTGGCGGCAGATGCTCACAGGTGAAGCGCAGG
TCGTCGTCGGCACCCGCTCGGCGGTTTTTGCGCCCTTACCCAAGCTGGGGCTGATTGTGCTTGATGAGGAGCACGACGGC
TCCTACAAGCAGGACCGTCCCGCCCCCTGCTACCACGCCCGGACTGTGGCGCTGTGGCGGGGCGAACTGGCAGGGTGCCC
GGTGCTGTTGGGTTCGGCCACCCCGGATCTCGAAAGTTTCGATCGCGCCACCGCAGGCCGCTACCTGCACCTGGAGATGC
CCGAGCGGGTGGCCGGGCGGCCTTTGCCCGCCGTCGAGGTGGTCGACATGCGCGAGGAACTGAACCGGGGCAACTTCACT
CCCTTTAGCGCCACGCTGCAGCGGGCGGTGGCCGAGATGCATGGGGCGGGCCGCCAGGGCATCTTGTTCATCAACCGCCG
CGGCTACAGCACCTTTGTGCTGTGCCGCAACTGCGGCGAGACGCTGCGCTGTCCCCACTGCGCCGTCTCGCTCACCTACC
ACCGCCTCGAAGCGGGCGACCATCTGCGCTGCCACTACTGCAACCACGGAGCCCCCCAGCCGCGCGCCTGTCCCCACTGC
ACCTCCCCCAACCTGCGCTACTTCGGGGCCGGTACCCAGCGCATCGCCGCCCAATTGAGCGAGCAATTTCCCACCCTGCG
GGTGCTGCGCTTCGACCGCGACACCACCGCCCGCAAGGACGCCCACCGCCAGATTCTTGAGCAGTTCGGCCGCGGGGAGG
CCGATGTCTTGGTAGGCACCCAAATGCTCACCAAGGGCCTCGATCTGCCCCAGGTGACCCTGGTGGGAATCCTGGCCGCC
GATGGACTGCTCAATTTGCCGGATTTTCGAGCCAGTGAGCGGGCTTTTCAGCTGCTCACCCAGGTGGCCGGGCGTGCCGG
GCGCGGCAGCGAACCCGGCCGGGTGATCCTTCAGACCTACGCCCCCGAGCATCCAGTGGTCGAAGCTGCCAGCACCCACG
ATTTTCGCCGCTACGCCGCCGCCGAACTCACCCAGCGCCGGGCCCTGGGCTATCCGCCTTTTGTCCAGCTGGTCGCCCTG
CAACTGAGCGCAGCGGAGCAACAGAGCGTCGTCGAGTCGGCCGAAGCCCTCGCTCGCCGCCTCGACGGCTCGGCGGATTT
TGAAGGTCGGCTTTTGGGTCCGGCCCCCTGCACCGTCGAGCGGGTCGCAGGCCGCTACCGCTGGCAGCTGCTCATCAAAA
ATCCGAACGGTGAGGCAGGCCGCGCCAGCCTGCGGGCCTTGCTCACCCAGTTTGTGCCCTGCGCGGGAGTGACGGTGGCG
GTGGATGTCGATCCGCTGCGGTTGCTCTAA

Upstream 100 bases:

>100_bases
TCGACCTGCGGCACACTCGGGCGTAAAGCCGTCCGGCGGTGTGCGGCACTCCAGATTTAGCGTGCCGACAGGTACCATAA
GCTAAGTCTGGATCGAGAGC

Downstream 100 bases:

>100_bases
CGACAAAAAAAAGTGAGGCCGACCGCCAGGGATAGCCTCACCCGCTCCTTTCAGCACCGGAAGTCCGGCTTGCCGTCTGC
CGGGACAAGCTTGTACATAG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 809; Mature: 809

Protein sequence:

>809_residues
METFRPGPLLRPAAWVQVLVDAPARERTYTYRLADGMTAAGGDVVCVPFGSQLVGGIVLGCLDQLPTGLDPGRLKTVESV
VGTGLFAPGFWPLLVRVADYYLVPLARVLETALPPGILSRARRRVRLVADAAPMAQWGLSAAAKSAFDFLRSQAHADFSW
RFCVQRLSGGVSSLRELQARRLLESYYVFGETARPRTQQFVVLAGAGADLCGRSAAVVQCLRRLGGEVSVEQLLAEARTT
RALLQKLDAGGHVRIYERQILRLAAPTASADAPKLLTDAQKQVLDRLQGATPGPVLLEGVTGSGKTEVYLQAIAPVLDRG
ESALVLVPEIGLTPQLTDRFAARFGARVRVYHSALSEGERFDCWRQMLTGEAQVVVGTRSAVFAPLPKLGLIVLDEEHDG
SYKQDRPAPCYHARTVALWRGELAGCPVLLGSATPDLESFDRATAGRYLHLEMPERVAGRPLPAVEVVDMREELNRGNFT
PFSATLQRAVAEMHGAGRQGILFINRRGYSTFVLCRNCGETLRCPHCAVSLTYHRLEAGDHLRCHYCNHGAPQPRACPHC
TSPNLRYFGAGTQRIAAQLSEQFPTLRVLRFDRDTTARKDAHRQILEQFGRGEADVLVGTQMLTKGLDLPQVTLVGILAA
DGLLNLPDFRASERAFQLLTQVAGRAGRGSEPGRVILQTYAPEHPVVEAASTHDFRRYAAAELTQRRALGYPPFVQLVAL
QLSAAEQQSVVESAEALARRLDGSADFEGRLLGPAPCTVERVAGRYRWQLLIKNPNGEAGRASLRALLTQFVPCAGVTVA
VDVDPLRLL

Sequences:

>Translated_809_residues
METFRPGPLLRPAAWVQVLVDAPARERTYTYRLADGMTAAGGDVVCVPFGSQLVGGIVLGCLDQLPTGLDPGRLKTVESV
VGTGLFAPGFWPLLVRVADYYLVPLARVLETALPPGILSRARRRVRLVADAAPMAQWGLSAAAKSAFDFLRSQAHADFSW
RFCVQRLSGGVSSLRELQARRLLESYYVFGETARPRTQQFVVLAGAGADLCGRSAAVVQCLRRLGGEVSVEQLLAEARTT
RALLQKLDAGGHVRIYERQILRLAAPTASADAPKLLTDAQKQVLDRLQGATPGPVLLEGVTGSGKTEVYLQAIAPVLDRG
ESALVLVPEIGLTPQLTDRFAARFGARVRVYHSALSEGERFDCWRQMLTGEAQVVVGTRSAVFAPLPKLGLIVLDEEHDG
SYKQDRPAPCYHARTVALWRGELAGCPVLLGSATPDLESFDRATAGRYLHLEMPERVAGRPLPAVEVVDMREELNRGNFT
PFSATLQRAVAEMHGAGRQGILFINRRGYSTFVLCRNCGETLRCPHCAVSLTYHRLEAGDHLRCHYCNHGAPQPRACPHC
TSPNLRYFGAGTQRIAAQLSEQFPTLRVLRFDRDTTARKDAHRQILEQFGRGEADVLVGTQMLTKGLDLPQVTLVGILAA
DGLLNLPDFRASERAFQLLTQVAGRAGRGSEPGRVILQTYAPEHPVVEAASTHDFRRYAAAELTQRRALGYPPFVQLVAL
QLSAAEQQSVVESAEALARRLDGSADFEGRLLGPAPCTVERVAGRYRWQLLIKNPNGEAGRASLRALLTQFVPCAGVTVA
VDVDPLRLL
>Mature_809_residues
METFRPGPLLRPAAWVQVLVDAPARERTYTYRLADGMTAAGGDVVCVPFGSQLVGGIVLGCLDQLPTGLDPGRLKTVESV
VGTGLFAPGFWPLLVRVADYYLVPLARVLETALPPGILSRARRRVRLVADAAPMAQWGLSAAAKSAFDFLRSQAHADFSW
RFCVQRLSGGVSSLRELQARRLLESYYVFGETARPRTQQFVVLAGAGADLCGRSAAVVQCLRRLGGEVSVEQLLAEARTT
RALLQKLDAGGHVRIYERQILRLAAPTASADAPKLLTDAQKQVLDRLQGATPGPVLLEGVTGSGKTEVYLQAIAPVLDRG
ESALVLVPEIGLTPQLTDRFAARFGARVRVYHSALSEGERFDCWRQMLTGEAQVVVGTRSAVFAPLPKLGLIVLDEEHDG
SYKQDRPAPCYHARTVALWRGELAGCPVLLGSATPDLESFDRATAGRYLHLEMPERVAGRPLPAVEVVDMREELNRGNFT
PFSATLQRAVAEMHGAGRQGILFINRRGYSTFVLCRNCGETLRCPHCAVSLTYHRLEAGDHLRCHYCNHGAPQPRACPHC
TSPNLRYFGAGTQRIAAQLSEQFPTLRVLRFDRDTTARKDAHRQILEQFGRGEADVLVGTQMLTKGLDLPQVTLVGILAA
DGLLNLPDFRASERAFQLLTQVAGRAGRGSEPGRVILQTYAPEHPVVEAASTHDFRRYAAAELTQRRALGYPPFVQLVAL
QLSAAEQQSVVESAEALARRLDGSADFEGRLLGPAPCTVERVAGRYRWQLLIKNPNGEAGRASLRALLTQFVPCAGVTVA
VDVDPLRLL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=809, Percent_Identity=36.7119901112485, Blast_Score=405, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 88095; Mature: 88095

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METFRPGPLLRPAAWVQVLVDAPARERTYTYRLADGMTAAGGDVVCVPFGSQLVGGIVLG
CCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCHHHHHHHHHH
CLDQLPTGLDPGRLKTVESVVGTGLFAPGFWPLLVRVADYYLVPLARVLETALPPGILSR
HHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ARRRVRLVADAAPMAQWGLSAAAKSAFDFLRSQAHADFSWRFCVQRLSGGVSSLRELQAR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHH
RLLESYYVFGETARPRTQQFVVLAGAGADLCGRSAAVVQCLRRLGGEVSVEQLLAEARTT
HHHHHHHHHCCCCCCCCCEEEEEECCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
RALLQKLDAGGHVRIYERQILRLAAPTASADAPKLLTDAQKQVLDRLQGATPGPVLLEGV
HHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECC
TGSGKTEVYLQAIAPVLDRGESALVLVPEIGLTPQLTDRFAARFGARVRVYHSALSEGER
CCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCH
FDCWRQMLTGEAQVVVGTRSAVFAPLPKLGLIVLDEEHDGSYKQDRPAPCYHARTVALWR
HHHHHHHHCCCCEEEEECCCHHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHEEEEEEC
GELAGCPVLLGSATPDLESFDRATAGRYLHLEMPERVAGRPLPAVEVVDMREELNRGNFT
CCCCCCCEEECCCCCCHHHHHHHHCCCEEEEECCHHHCCCCCCHHHHHHHHHHHCCCCCC
PFSATLQRAVAEMHGAGRQGILFINRRGYSTFVLCRNCGETLRCPHCAVSLTYHRLEAGD
HHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCEEEEEHHEECCCC
HLRCHYCNHGAPQPRACPHCTSPNLRYFGAGTQRIAAQLSEQFPTLRVLRFDRDTTARKD
CEEEEECCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCEEEEEECCCCHHHHH
AHRQILEQFGRGEADVLVGTQMLTKGLDLPQVTLVGILAADGLLNLPDFRASERAFQLLT
HHHHHHHHHCCCCCEEEECHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
QVAGRAGRGSEPGRVILQTYAPEHPVVEAASTHDFRRYAAAELTQRRALGYPPFVQLVAL
HHHCCCCCCCCCCEEEEEECCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
QLSAAEQQSVVESAEALARRLDGSADFEGRLLGPAPCTVERVAGRYRWQLLIKNPNGEAG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHCCCEEEEEEEECCCCCHH
RASLRALLTQFVPCAGVTVAVDVDPLRLL
HHHHHHHHHHHCCCCCCEEEEECCHHHCC
>Mature Secondary Structure
METFRPGPLLRPAAWVQVLVDAPARERTYTYRLADGMTAAGGDVVCVPFGSQLVGGIVLG
CCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCHHHHHHHHHH
CLDQLPTGLDPGRLKTVESVVGTGLFAPGFWPLLVRVADYYLVPLARVLETALPPGILSR
HHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ARRRVRLVADAAPMAQWGLSAAAKSAFDFLRSQAHADFSWRFCVQRLSGGVSSLRELQAR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHH
RLLESYYVFGETARPRTQQFVVLAGAGADLCGRSAAVVQCLRRLGGEVSVEQLLAEARTT
HHHHHHHHHCCCCCCCCCEEEEEECCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
RALLQKLDAGGHVRIYERQILRLAAPTASADAPKLLTDAQKQVLDRLQGATPGPVLLEGV
HHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECC
TGSGKTEVYLQAIAPVLDRGESALVLVPEIGLTPQLTDRFAARFGARVRVYHSALSEGER
CCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCH
FDCWRQMLTGEAQVVVGTRSAVFAPLPKLGLIVLDEEHDGSYKQDRPAPCYHARTVALWR
HHHHHHHHCCCCEEEEECCCHHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHEEEEEEC
GELAGCPVLLGSATPDLESFDRATAGRYLHLEMPERVAGRPLPAVEVVDMREELNRGNFT
CCCCCCCEEECCCCCCHHHHHHHHCCCEEEEECCHHHCCCCCCHHHHHHHHHHHCCCCCC
PFSATLQRAVAEMHGAGRQGILFINRRGYSTFVLCRNCGETLRCPHCAVSLTYHRLEAGD
HHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCEEEEEHHEECCCC
HLRCHYCNHGAPQPRACPHCTSPNLRYFGAGTQRIAAQLSEQFPTLRVLRFDRDTTARKD
CEEEEECCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCEEEEEECCCCHHHHH
AHRQILEQFGRGEADVLVGTQMLTKGLDLPQVTLVGILAADGLLNLPDFRASERAFQLLT
HHHHHHHHHCCCCCEEEECHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
QVAGRAGRGSEPGRVILQTYAPEHPVVEAASTHDFRRYAAAELTQRRALGYPPFVQLVAL
HHHCCCCCCCCCCEEEEEECCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
QLSAAEQQSVVESAEALARRLDGSADFEGRLLGPAPCTVERVAGRYRWQLLIKNPNGEAG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHCCCEEEEEEEECCCCCHH
RASLRALLTQFVPCAGVTVAVDVDPLRLL
HHHHHHHHHHHCCCCCCEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]