Click here to switch to the map view.

The map label for this gene is trmE

Identifier: 34499858

GI number: 34499858

Start: 4747189

End: 4748541

Strand: Reverse

Name: trmE

Synonym: CV_4403

Alternate gene names: 34499858

Gene position: 4748541-4747189 (Counterclockwise)

Preceding gene: 34499859

Following gene: 34499857

Centisome position: 99.95

GC content: 68.29

Gene sequence:

>1353_bases
ATGAACCTCAGCTACACCCCCGCCACCATCTGCGCCGTCGCCACCGCTCCGGGACGCGGCGGCGTCGGCGTGATCCGCGT
GTCCGGCAAGGACCTGCTGCCGTTCGCGCAGGCTATTTCCGGCGGCAAGACGCCCAAGCCGCGCTACGCAACGTACACCG
ACTTTTTCGACGCCCACGGCCAGGCGCTGGACAACGGCCTGCTGCTGTTCTTCCCCGGTCCCAACAGCTTCACCGGCGAG
GACGTGATCGAGCTGCAGGGCCACGGCGGGCCGGTGGTGCTGAAGATGCTGCTGGCGCGCTGCGTGGAGCTGGGCGCGCG
GCTGGCCGAGCCGGGCGAATTCACCAAGCGGGCGTTCTTGAACGACAAGCTGGACCTGGCCCAGGCCGAGAGCGTGGCCG
ACCTGATCGACGCCAGCAGCGAAACGGCGGCCAGGAGCGCGCTGAAATCGCTGAAGGGCGCGTTCTCGCGCGAGGTGCAC
GGCCTGGTAGACGAGCTGATCAACCTGCGCATGCTGGTGGAGGCGACGCTGGACTTCCCGGAAGAAGAGATAGATTTCCT
GAAGCAGGCCGATGCGATCGGCCGCCTGCGCCGGCTGCGCGCGCAGCTGGTGGGGGTGCAGGCCACCGCCAAGCAGGGTG
CCATCCTGCGCGAGGGCATGCACGTGGTGCTGGTGGGCCAGCCCAATGTCGGCAAGTCCAGCCTGATGAACGCGCTGGCC
GGCGACGACATCGCCATCGTCACCGATATCGCCGGCACTACCCGCGACACGGTGCGCGAGGAGATCGTCATCGACGGCGT
GCCGGTGCACATCATCGATACCGCCGGCCTGCGCGACACCGACGACGTGGTGGAGAAGATAGGCATCGAGCGTACCTGGC
AGGCGGTGGAGCGCGCCGACCTCGCCTTGCTGCTGGTGGACAGCCGCGAAGGCCTGACCGCCGAGGTGCAGTCCATCCTG
GAGCGGCTGCCGCCGGCGCTGCCCAGGGTGCAGGTCTTCAACAAAGTGGACCTGTCCGGCGAGGCCGCCGGTCTGGCCGA
GCAGGACGGCCACCCGCTGGTGCGCCTGTCCGCCCGCACCCACGACGGCGTGGACATCCTCAAGGCCAAGCTGCTGGAGA
TGATAGGCTACAGCGGCGCCGACGAGGGTGTGTTCCTGGCCCGCCAGCGCCACCTGGACGCCATCGCCCGCGCCGCCGAC
CACCTGGAACTGGCCGAAGCCGACTGGGAGCAGGTGGAAATCTTCGCTGAGGAATTGCGGATGGCGCAGAACGCGCTGTC
CGAGATCACCGGCGAGTTCAGCGCCGACGACCTGCTGGGCGTGATCTTCTCGCGCTTCTGCATCGGCAAGTAG

Upstream 100 bases:

>100_bases
CGTCCGAATTGCTGATGGAACCCGGCCACTGGCCGGGTTTTTTGCTGGATAATTGAGTGATGATAGCCGCAGCCGGCCGC
CGGCTGCCCAAGGAATGCCG

Downstream 100 bases:

>100_bases
ACCCCGAATCGCGCCGCGTCCCTGCGGACGTCCGTACGCAAAGCCCCGCCAGCCGGGGCTTTTTTCATTCCGGCTGTCCT
TGTGATTTATATATCTAAAA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MNLSYTPATICAVATAPGRGGVGVIRVSGKDLLPFAQAISGGKTPKPRYATYTDFFDAHGQALDNGLLLFFPGPNSFTGE
DVIELQGHGGPVVLKMLLARCVELGARLAEPGEFTKRAFLNDKLDLAQAESVADLIDASSETAARSALKSLKGAFSREVH
GLVDELINLRMLVEATLDFPEEEIDFLKQADAIGRLRRLRAQLVGVQATAKQGAILREGMHVVLVGQPNVGKSSLMNALA
GDDIAIVTDIAGTTRDTVREEIVIDGVPVHIIDTAGLRDTDDVVEKIGIERTWQAVERADLALLLVDSREGLTAEVQSIL
ERLPPALPRVQVFNKVDLSGEAAGLAEQDGHPLVRLSARTHDGVDILKAKLLEMIGYSGADEGVFLARQRHLDAIARAAD
HLELAEADWEQVEIFAEELRMAQNALSEITGEFSADDLLGVIFSRFCIGK

Sequences:

>Translated_450_residues
MNLSYTPATICAVATAPGRGGVGVIRVSGKDLLPFAQAISGGKTPKPRYATYTDFFDAHGQALDNGLLLFFPGPNSFTGE
DVIELQGHGGPVVLKMLLARCVELGARLAEPGEFTKRAFLNDKLDLAQAESVADLIDASSETAARSALKSLKGAFSREVH
GLVDELINLRMLVEATLDFPEEEIDFLKQADAIGRLRRLRAQLVGVQATAKQGAILREGMHVVLVGQPNVGKSSLMNALA
GDDIAIVTDIAGTTRDTVREEIVIDGVPVHIIDTAGLRDTDDVVEKIGIERTWQAVERADLALLLVDSREGLTAEVQSIL
ERLPPALPRVQVFNKVDLSGEAAGLAEQDGHPLVRLSARTHDGVDILKAKLLEMIGYSGADEGVFLARQRHLDAIARAAD
HLELAEADWEQVEIFAEELRMAQNALSEITGEFSADDLLGVIFSRFCIGK
>Mature_450_residues
MNLSYTPATICAVATAPGRGGVGVIRVSGKDLLPFAQAISGGKTPKPRYATYTDFFDAHGQALDNGLLLFFPGPNSFTGE
DVIELQGHGGPVVLKMLLARCVELGARLAEPGEFTKRAFLNDKLDLAQAESVADLIDASSETAARSALKSLKGAFSREVH
GLVDELINLRMLVEATLDFPEEEIDFLKQADAIGRLRRLRAQLVGVQATAKQGAILREGMHVVLVGQPNVGKSSLMNALA
GDDIAIVTDIAGTTRDTVREEIVIDGVPVHIIDTAGLRDTDDVVEKIGIERTWQAVERADLALLLVDSREGLTAEVQSIL
ERLPPALPRVQVFNKVDLSGEAAGLAEQDGHPLVRLSARTHDGVDILKAKLLEMIGYSGADEGVFLARQRHLDAIARAAD
HLELAEADWEQVEIFAEELRMAQNALSEITGEFSADDLLGVIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=469, Percent_Identity=36.4605543710021, Blast_Score=218, Evalue=7e-57,
Organism=Homo sapiens, GI193082993, Length=452, Percent_Identity=36.0619469026549, Blast_Score=209, Evalue=5e-54,
Organism=Homo sapiens, GI193082988, Length=492, Percent_Identity=33.9430894308943, Blast_Score=205, Evalue=9e-53,
Organism=Escherichia coli, GI2367268, Length=451, Percent_Identity=58.5365853658537, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI87082120, Length=104, Percent_Identity=40.3846153846154, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17507259, Length=455, Percent_Identity=30.989010989011, Blast_Score=183, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6323665, Length=494, Percent_Identity=31.7813765182186, Blast_Score=211, Evalue=2e-55,
Organism=Drosophila melanogaster, GI45550813, Length=479, Percent_Identity=35.6993736951983, Blast_Score=221, Evalue=8e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_CHRVO (Q7NPT9)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_904073.1
- ProteinModelPortal:   Q7NPT9
- SMR:   Q7NPT9
- GeneID:   2549694
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4403
- NMPDR:   fig|243365.1.peg.4403
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   Q7NPT9
- ProtClustDB:   PRK05291
- BioCyc:   CVIO243365:CV_4403-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 48426; Mature: 48426

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: BINDING 26-26 BINDING 84-84 BINDING 123-123 BINDING 450-450

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSYTPATICAVATAPGRGGVGVIRVSGKDLLPFAQAISGGKTPKPRYATYTDFFDAHG
CCCCCCCHHHEEEEECCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHCC
QALDNGLLLFFPGPNSFTGEDVIELQGHGGPVVLKMLLARCVELGARLAEPGEFTKRAFL
HHCCCCEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
NDKLDLAQAESVADLIDASSETAARSALKSLKGAFSREVHGLVDELINLRMLVEATLDFP
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EEEIDFLKQADAIGRLRRLRAQLVGVQATAKQGAILREGMHVVLVGQPNVGKSSLMNALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEECCCCCHHHHHHHHC
GDDIAIVTDIAGTTRDTVREEIVIDGVPVHIIDTAGLRDTDDVVEKIGIERTWQAVERAD
CCCEEEEEECCCCCHHHHHHHHEECCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHCC
LALLLVDSREGLTAEVQSILERLPPALPRVQVFNKVDLSGEAAGLAEQDGHPLVRLSART
EEEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEECCC
HDGVDILKAKLLEMIGYSGADEGVFLARQRHLDAIARAADHLELAEADWEQVEIFAEELR
CCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
MAQNALSEITGEFSADDLLGVIFSRFCIGK
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLSYTPATICAVATAPGRGGVGVIRVSGKDLLPFAQAISGGKTPKPRYATYTDFFDAHG
CCCCCCCHHHEEEEECCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHCC
QALDNGLLLFFPGPNSFTGEDVIELQGHGGPVVLKMLLARCVELGARLAEPGEFTKRAFL
HHCCCCEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
NDKLDLAQAESVADLIDASSETAARSALKSLKGAFSREVHGLVDELINLRMLVEATLDFP
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EEEIDFLKQADAIGRLRRLRAQLVGVQATAKQGAILREGMHVVLVGQPNVGKSSLMNALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEECCCCCHHHHHHHHC
GDDIAIVTDIAGTTRDTVREEIVIDGVPVHIIDTAGLRDTDDVVEKIGIERTWQAVERAD
CCCEEEEEECCCCCHHHHHHHHEECCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHCC
LALLLVDSREGLTAEVQSILERLPPALPRVQVFNKVDLSGEAAGLAEQDGHPLVRLSART
EEEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEECCC
HDGVDILKAKLLEMIGYSGADEGVFLARQRHLDAIARAADHLELAEADWEQVEIFAEELR
CCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
MAQNALSEITGEFSADDLLGVIFSRFCIGK
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782