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The map label for this gene is bioH

Identifier: 34499832

GI number: 34499832

Start: 4719609

End: 4720328

Strand: Reverse

Name: bioH

Synonym: CV_4377

Alternate gene names: 34499832

Gene position: 4720328-4719609 (Counterclockwise)

Preceding gene: 34499833

Following gene: 34499831

Centisome position: 99.35

GC content: 72.64

Gene sequence:

>720_bases
ATGCTGCACGGCTGGGGGCTGCACGGCGGCGTGTTCGCCCGCGTGGCCGAGCAACTGGCGACGCGTTTCTGCGTCCACCT
GGTGGATCTGCCGGGCCACGGCGCGTCGCCGGCGCTGCCGCGCTTCGACGCCGACGCGGTGGCCGATCTGCTCGCCGCCC
ATTTCCCCTTGCCCGCGCAGGTGGTGGGCTGGTCGCTTGGCGGCCTGATCGCCCAGCACTGGGCCGCCCGCCATCCGGAC
AAGGTGAAGAGCCTGGCGCTGGTGGCCACCAGCCCGCGCTTCGTCCGCGACGAGACCTGGCCGCACGCGCAGGCGCGCGC
ATCCATCGAGGCGGTGGCGCAGAGCCTGGACGGCGCGTTCGAGCAGACGCTGGAGCGCTTCCTCGCCTTGCAGATGATGG
GCGCCCCGTCGGCGCGCGACACGCTGAAGGCCTTGCGCGGCGAACTGTTTTCCCATGGCCGGCCGCAGGGCCTGCTGCCG
GCGCTGGGGCTGCTGCTGGAGGCCGACGCGCGCGCGCTGGCCGGCCGCATCCAGTGCCCGGCCGCGCTGTTCTACGGCGC
GCGCGACGCCATCACCCCGATCGGCGCCGGCCGCTGGCTGGCCGAGTCGCTGCCCGACGCCGTCCTTTATGAATTCCCGC
AAGCGTCGCACGCCCCCTTCCTGTCGCATGAACAGGATTTCGTGCGCGCGCTTGCCGAGCATCTGGAAACCCAGGCATGA

Upstream 100 bases:

>100_bases
TTTCCTTCGCATCGTACTCTTGCGAAAATAGCGTTTTCGCCCAATTTATCCGCACATGAATCTGTTTGTCGAAACGCTGG
GCCAGGGGCCGGACGTGGTC

Downstream 100 bases:

>100_bases
GTGAAGCGTTTTACACCGACAAGGCGCGCGTGCGCGCCTCGTTCGAAAAGGCCGCGGCCAGCTACGATTCCGCCGCGGTG
CTGCAGCGCGAAGTCTCCGA

Product: BioH protein

Products: NA

Alternate protein names: Biotin synthesis protein BioH

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MLHGWGLHGGVFARVAEQLATRFCVHLVDLPGHGASPALPRFDADAVADLLAAHFPLPAQVVGWSLGGLIAQHWAARHPD
KVKSLALVATSPRFVRDETWPHAQARASIEAVAQSLDGAFEQTLERFLALQMMGAPSARDTLKALRGELFSHGRPQGLLP
ALGLLLEADARALAGRIQCPAALFYGARDAITPIGAGRWLAESLPDAVLYEFPQASHAPFLSHEQDFVRALAEHLETQA

Sequences:

>Translated_239_residues
MLHGWGLHGGVFARVAEQLATRFCVHLVDLPGHGASPALPRFDADAVADLLAAHFPLPAQVVGWSLGGLIAQHWAARHPD
KVKSLALVATSPRFVRDETWPHAQARASIEAVAQSLDGAFEQTLERFLALQMMGAPSARDTLKALRGELFSHGRPQGLLP
ALGLLLEADARALAGRIQCPAALFYGARDAITPIGAGRWLAESLPDAVLYEFPQASHAPFLSHEQDFVRALAEHLETQA
>Mature_239_residues
MLHGWGLHGGVFARVAEQLATRFCVHLVDLPGHGASPALPRFDADAVADLLAAHFPLPAQVVGWSLGGLIAQHWAARHPD
KVKSLALVATSPRFVRDETWPHAQARASIEAVAQSLDGAFEQTLERFLALQMMGAPSARDTLKALRGELFSHGRPQGLLP
ALGLLLEADARALAGRIQCPAALFYGARDAITPIGAGRWLAESLPDAVLYEFPQASHAPFLSHEQDFVRALAEHLETQA

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family

Homologues:

Organism=Escherichia coli, GI1789817, Length=234, Percent_Identity=35.4700854700855, Blast_Score=135, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOH_CHRVO (Q7NPW5)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_904047.1
- ProteinModelPortal:   Q7NPW5
- GeneID:   2549766
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4377
- NMPDR:   fig|243365.1.peg.4377
- HOGENOM:   HBG748003
- OMA:   CTIASSP
- PhylomeDB:   Q7NPW5
- ProtClustDB:   CLSK2485742
- BioCyc:   CVIO243365:CV_4377-MONOMER
- BRENDA:   3.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_01260
- InterPro:   IPR000073
- InterPro:   IPR010076
- TIGRFAMs:   TIGR01738

Pfam domain/function: PF00561 Abhydrolase_1

EC number: =3.1.1.1

Molecular weight: Translated: 25701; Mature: 25701

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: ACT_SITE 80-80 ACT_SITE 204-204 ACT_SITE 232-232

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHGWGLHGGVFARVAEQLATRFCVHLVDLPGHGASPALPRFDADAVADLLAAHFPLPAQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHH
VVGWSLGGLIAQHWAARHPDKVKSLALVATSPRFVRDETWPHAQARASIEAVAQSLDGAF
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHH
EQTLERFLALQMMGAPSARDTLKALRGELFSHGRPQGLLPALGLLLEADARALAGRIQCP
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCH
AALFYGARDAITPIGAGRWLAESLPDAVLYEFPQASHAPFLSHEQDFVRALAEHLETQA
HHHHHCCHHHCCCCCCCHHHHHHCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLHGWGLHGGVFARVAEQLATRFCVHLVDLPGHGASPALPRFDADAVADLLAAHFPLPAQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHH
VVGWSLGGLIAQHWAARHPDKVKSLALVATSPRFVRDETWPHAQARASIEAVAQSLDGAF
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHH
EQTLERFLALQMMGAPSARDTLKALRGELFSHGRPQGLLPALGLLLEADARALAGRIQCP
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCH
AALFYGARDAITPIGAGRWLAESLPDAVLYEFPQASHAPFLSHEQDFVRALAEHLETQA
HHHHHCCHHHCCCCCCCHHHHHHCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782