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The map label for this gene is mraW

Identifier: 34499806

GI number: 34499806

Start: 4692232

End: 4693194

Strand: Reverse

Name: mraW

Synonym: CV_4351

Alternate gene names: 34499806

Gene position: 4693194-4692232 (Counterclockwise)

Preceding gene: 34499807

Following gene: 34499805

Centisome position: 98.78

GC content: 67.5

Gene sequence:

>963_bases
GTGAGCACCCCCACCTTCGTGCATCGCACGGTATTGCTGACCGAAGCGGTCGACGCGCTCGCCATTCGTCCCGATGGCGT
CTACGTCGATTGCACCTTCGGTCGCGGAGGCCACAGCCGCCTGATCCTGTCGAAGCTCGGCCCCTCCGGCCGGCTGATCG
CCTTCGACAAGGATCCGGAAGCCATCGCCGTCGCCGACAGGCTGGCGGCGGAGGATTCCCGCTTCAACATCGTGCACAAC
GGTTTCGAGACGCTGTCGGCCGAGCTCGCCCGCCTGGGCGTGGCGGGCGTCGACGGCGTGCTGATGGACCTGGGCGTGTC
GTCGCCGCAGATCGACGACGGCAGCCGCGGCTTCAGCTTCCGCTTCGACGCGCCGCTGGACATGCGTATGGATACCACCC
GCGGCGTCACCGCCGCCGAGTGGTTGGCAACGGCCGACGAGGCCGATATCAGAGAGGTCATCAAGACTTATGGTGAAGAG
CGGTTTGCTCGCAAGATCGCAGCAGCCATTGTTGCGCAACGGGACGAAAGCCCCATCACGACCACCCGCGAGCTCGCTGT
GCTCGTTGGGCAAAACGTCCGTACTCGCGAACCGGGTCAAGACCCGGCGACGCGAACCTTCCAGGCGATCCGGATCTTTG
TGAACCGCGAGCTGGACGAGCTGAAGGCGGTGCTGCCGCAGGCCGCGCGCCTCTTGAACGAGGGCGGCCGCTTGGCGGTG
ATCAGCTTCCATTCGCTGGAGGACCGCATCGTCAAGCTCTACCTGCGCGACGTCAGCAGCGAGGAAAAGCTGCCGGCCTG
GGCGATGGTGCGCGCGGCCGACATGGCCCAGCCGCCGATAGACCTGGTCGGCAAGGCCATCCGCGCCGGCGACGAGGAAG
TGCGCGAGAATCCGCGCGCCCGCAGCGCCATCATGCGCGTGGCCCAGCGCACCGCCGCGCCGTGGCGGGAGGGCGACGCA
TGA

Upstream 100 bases:

>100_bases
GCTTCGAACTGTGGAATGCCGAGGAATGGGACAGCCAGACCGCTGACGCACTGGCCATCGACCAAGCCGATTTGGCGCAA
CACCTTGGAGATTTCACGCT

Downstream 100 bases:

>100_bases
ACAAGCTCAACGCGACGCTGCTGCTGATGGCCGTGTTCTCCGCCTGGTCGGTGGTGACGTCCACGCACGTGTCGCGCAAG
CTCTACAGCGACTTGCAGAA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSTPTFVHRTVLLTEAVDALAIRPDGVYVDCTFGRGGHSRLILSKLGPSGRLIAFDKDPEAIAVADRLAAEDSRFNIVHN
GFETLSAELARLGVAGVDGVLMDLGVSSPQIDDGSRGFSFRFDAPLDMRMDTTRGVTAAEWLATADEADIREVIKTYGEE
RFARKIAAAIVAQRDESPITTTRELAVLVGQNVRTREPGQDPATRTFQAIRIFVNRELDELKAVLPQAARLLNEGGRLAV
ISFHSLEDRIVKLYLRDVSSEEKLPAWAMVRAADMAQPPIDLVGKAIRAGDEEVRENPRARSAIMRVAQRTAAPWREGDA

Sequences:

>Translated_320_residues
MSTPTFVHRTVLLTEAVDALAIRPDGVYVDCTFGRGGHSRLILSKLGPSGRLIAFDKDPEAIAVADRLAAEDSRFNIVHN
GFETLSAELARLGVAGVDGVLMDLGVSSPQIDDGSRGFSFRFDAPLDMRMDTTRGVTAAEWLATADEADIREVIKTYGEE
RFARKIAAAIVAQRDESPITTTRELAVLVGQNVRTREPGQDPATRTFQAIRIFVNRELDELKAVLPQAARLLNEGGRLAV
ISFHSLEDRIVKLYLRDVSSEEKLPAWAMVRAADMAQPPIDLVGKAIRAGDEEVRENPRARSAIMRVAQRTAAPWREGDA
>Mature_319_residues
STPTFVHRTVLLTEAVDALAIRPDGVYVDCTFGRGGHSRLILSKLGPSGRLIAFDKDPEAIAVADRLAAEDSRFNIVHNG
FETLSAELARLGVAGVDGVLMDLGVSSPQIDDGSRGFSFRFDAPLDMRMDTTRGVTAAEWLATADEADIREVIKTYGEER
FARKIAAAIVAQRDESPITTTRELAVLVGQNVRTREPGQDPATRTFQAIRIFVNRELDELKAVLPQAARLLNEGGRLAVI
SFHSLEDRIVKLYLRDVSSEEKLPAWAMVRAADMAQPPIDLVGKAIRAGDEEVRENPRARSAIMRVAQRTAAPWREGDA

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=335, Percent_Identity=38.8059701492537, Blast_Score=195, Evalue=4e-50,
Organism=Homo sapiens, GI165377202, Length=198, Percent_Identity=40.9090909090909, Blast_Score=131, Evalue=1e-30,
Organism=Escherichia coli, GI1786270, Length=316, Percent_Identity=54.4303797468354, Blast_Score=328, Evalue=4e-91,
Organism=Drosophila melanogaster, GI62472493, Length=334, Percent_Identity=38.0239520958084, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI28571637, Length=334, Percent_Identity=38.0239520958084, Blast_Score=173, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_CHRVO (Q7NPZ1)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_904021.1
- ProteinModelPortal:   Q7NPZ1
- SMR:   Q7NPZ1
- GeneID:   2549809
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4351
- NMPDR:   fig|243365.1.peg.4351
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- PhylomeDB:   Q7NPZ1
- ProtClustDB:   PRK00050
- BioCyc:   CVIO243365:CV_4351-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35059; Mature: 34928

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: BINDING 56-56 BINDING 82-82 BINDING 103-103 BINDING 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPTFVHRTVLLTEAVDALAIRPDGVYVDCTFGRGGHSRLILSKLGPSGRLIAFDKDPE
CCCCHHHHHHHHHHHHHHHEEECCCCEEEEEECCCCCCCEEEEECCCCCCCEEEECCCCC
AIAVADRLAAEDSRFNIVHNGFETLSAELARLGVAGVDGVLMDLGVSSPQIDDGSRGFSF
HHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCEEE
RFDAPLDMRMDTTRGVTAAEWLATADEADIREVIKTYGEERFARKIAAAIVAQRDESPIT
EECCCCCCEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TTRELAVLVGQNVRTREPGQDPATRTFQAIRIFVNRELDELKAVLPQAARLLNEGGRLAV
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEE
ISFHSLEDRIVKLYLRDVSSEEKLPAWAMVRAADMAQPPIDLVGKAIRAGDEEVRENPRA
EEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHCCHH
RSAIMRVAQRTAAPWREGDA
HHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
STPTFVHRTVLLTEAVDALAIRPDGVYVDCTFGRGGHSRLILSKLGPSGRLIAFDKDPE
CCCHHHHHHHHHHHHHHHEEECCCCEEEEEECCCCCCCEEEEECCCCCCCEEEECCCCC
AIAVADRLAAEDSRFNIVHNGFETLSAELARLGVAGVDGVLMDLGVSSPQIDDGSRGFSF
HHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCEEE
RFDAPLDMRMDTTRGVTAAEWLATADEADIREVIKTYGEERFARKIAAAIVAQRDESPIT
EECCCCCCEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TTRELAVLVGQNVRTREPGQDPATRTFQAIRIFVNRELDELKAVLPQAARLLNEGGRLAV
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEE
ISFHSLEDRIVKLYLRDVSSEEKLPAWAMVRAADMAQPPIDLVGKAIRAGDEEVRENPRA
EEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHCCHH
RSAIMRVAQRTAAPWREGDA
HHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782