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The map label for this gene is preP [C]

Identifier: 34499761

GI number: 34499761

Start: 4647695

End: 4649728

Strand: Reverse

Name: preP [C]

Synonym: CV_4306

Alternate gene names: 34499761

Gene position: 4649728-4647695 (Counterclockwise)

Preceding gene: 34499762

Following gene: 34499760

Centisome position: 97.87

GC content: 70.26

Gene sequence:

>2034_bases
ATGAACCCTTCCCACGACCCCCATGCCTGGCTGGAAAGCCTGGATTCCGACCAAGCCGCCGCCTGGGTGGCGGAGCAGAA
CGCCCGCACCGAGGGCGTGCTGGACGCCGATCCGCGCTTCGCGGGCCTCAAGGCCGACATCCTCGCCCATCTGCGCGACA
CGCGGCAGATCCCGTACTTCGCCGAGCACAAAGGGTGGCTGTACAACTTCCACCAGGACGAGGCGCATCCGCGCGGCATT
TACCGCCGCACCACGCTGGACGCCTACCGCGCCGGCAGCCAGGACTGGCAGACGGTGCTCGACATCGACGCGCTGGCCGA
CGCCGACGAGCGCGACTGGTATCTGGACGGCGTGTCGCATTGCACGGTCAGGCCGGAGCGGGCGCTGGTGCATCTGACCG
ACGGCGGCGGCGACGCCACCGTGGCGCGCGAGTACGATGTCGAGACGCGGCGCTGGGTGGAGGACGGCTTCAATTTCCCC
GAGGGCAAGAACCACATCGCCTGGCGCGATCCGGACAGCGCCTTTGTCTGCCCAGGCTGGAAGGGCGCGCCGCTGACCCG
CGCCGGCTACCCGCGCGAGGTGTGGCTGGTGGAGCGCGCCGCCGATGGCCGGCACGGCTGGACCCTGCTGTTCGCCGCGC
CGGAGGACGCGATGATGACCGCCGCCTGGCGCTTTCTCGACAGCGACGGCAGCGCCGTGGACCTGATCGAGGCGTCCGAC
GGTTTCTACCGCAAGACGTATCACCTGGTGGACGGCGGCCTGGAGGCGCATGCGCTGCCGCTGCCGGCCAAGGCCGAGAT
CGAGGCCTATATCGCAGGCGATTTCATCGTCAAGCTGGCCGAGGACTGGCGCTGGCGCGGCGAAGCGTATCCCAGCGGCA
GTCTGTTGGCGGTGGGCCTGCCCGGGTTGATCGGCGAGGGTCCGGCGCCGCAGCTGCTGCAGGCGCCCGCGCCCAGGCTG
GCGGTGGCGGCGGTGGAAACCACCCGCAGCCGGCTGCTGGTCAATCTGCTGGACAATGTCAAAAGCCGCATGCTGTGCTT
CGCCAAGCGCGACGGCGCGTGGGAGACGCAGCCGTTGGCGCTGCCGGCCGACGGCGTGATCGAGATCGCCGACCAGCCGT
GGCAGAGCGACGTGCTCTATTACAGCTACAGCGACTTCCTGACGCCTAGCGGCCTGTACCGGCTGGACTTGGAAAGCGGC
GACAGCGAATGCCTGCGCCGGCAGCCGGAAGCCTTCGATCCGGCGCCCTACGTCGCCGAGCAATGGCAGGCGACGGCGCC
GGACGGCGAGCGGATTCCGTACTTCGTGGTGAGGCGGCGCGACGCGCCGTTCGACGGCGCGACGCCGACGCTGCTGTATG
GCTATGGCGGCTTCGAGGTGCCGATGCTGCCGTACTACATCGAGAACTTCGGGCCGCACTGGCTGGCCAAGGGCGGCGCC
TTCGTGCTGGCCTGCATCCGCGGCGGCGGCGAGTTCGGCCCGCACTGGCACCAGGCGGCGCAAGGGCCGAAGCGCCAGGT
CAGCTTCGACGACTTCATCGCGGTGGCGGAAGACCTGATCGCCCGCAAGCTGACCTCGCCGGCCAGGCTGGCGATAGAGG
GCGGCAGCAACGGCGGCCTCTTGGTGGGCGCGGCGATGGCGCAGCGGCCGGAGCTGTTCCGGGCCGTGGTGTGCGAGGTG
CCCTTGCTGGACATGCTGCGCTACACCCAGCTGTTGGCAGGCGCCAGCTGGATAGACGAGTACGGCGATCCGGACGACGA
GGCCGAGCGCGCCGCGCTGGCCGCCTATTCGCCGTACCACAATCTGCGCGCCGACGCGCGCTACCCGCTGGCCTTGTTCA
CCACCAGCGCCAGCGACGACCGGGTGCATCCCGGCCACGCCCGCAAGATGGCGGCGCGGCTGCTGGCGCTGGGCCACGAC
GCGCTGTTCTACGAGACCGACGGCGGCGGCCACGCCGGCAATGCCGGCCAGGAGGACACCGCCGCCGAGCTGGCGCGGGT
GCTGGTCTACCTCTACCAGCGCTTGTGCGATTGA

Upstream 100 bases:

>100_bases
CGCCTGGATGCTGGCGCATGGCCGGCATTGAGGCGATTTGGCAGCCGCGCGCGCCGATGCTACCCTCGCCGCCATCCTCA
GATTTTCTATCCCGATCGCC

Downstream 100 bases:

>100_bases
GGCGGCAGAGGTGGCAGCAAAGGGCGCGAAAGCGCCCTTTTTCATGGCGGCTTGCCTCTTTGGGCCGGTTTGCCGAACGA
TGATGTCGTTTGCATTTTGC

Product: prolyl endopeptidase

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 677; Mature: 677

Protein sequence:

>677_residues
MNPSHDPHAWLESLDSDQAAAWVAEQNARTEGVLDADPRFAGLKADILAHLRDTRQIPYFAEHKGWLYNFHQDEAHPRGI
YRRTTLDAYRAGSQDWQTVLDIDALADADERDWYLDGVSHCTVRPERALVHLTDGGGDATVAREYDVETRRWVEDGFNFP
EGKNHIAWRDPDSAFVCPGWKGAPLTRAGYPREVWLVERAADGRHGWTLLFAAPEDAMMTAAWRFLDSDGSAVDLIEASD
GFYRKTYHLVDGGLEAHALPLPAKAEIEAYIAGDFIVKLAEDWRWRGEAYPSGSLLAVGLPGLIGEGPAPQLLQAPAPRL
AVAAVETTRSRLLVNLLDNVKSRMLCFAKRDGAWETQPLALPADGVIEIADQPWQSDVLYYSYSDFLTPSGLYRLDLESG
DSECLRRQPEAFDPAPYVAEQWQATAPDGERIPYFVVRRRDAPFDGATPTLLYGYGGFEVPMLPYYIENFGPHWLAKGGA
FVLACIRGGGEFGPHWHQAAQGPKRQVSFDDFIAVAEDLIARKLTSPARLAIEGGSNGGLLVGAAMAQRPELFRAVVCEV
PLLDMLRYTQLLAGASWIDEYGDPDDEAERAALAAYSPYHNLRADARYPLALFTTSASDDRVHPGHARKMAARLLALGHD
ALFYETDGGGHAGNAGQEDTAAELARVLVYLYQRLCD

Sequences:

>Translated_677_residues
MNPSHDPHAWLESLDSDQAAAWVAEQNARTEGVLDADPRFAGLKADILAHLRDTRQIPYFAEHKGWLYNFHQDEAHPRGI
YRRTTLDAYRAGSQDWQTVLDIDALADADERDWYLDGVSHCTVRPERALVHLTDGGGDATVAREYDVETRRWVEDGFNFP
EGKNHIAWRDPDSAFVCPGWKGAPLTRAGYPREVWLVERAADGRHGWTLLFAAPEDAMMTAAWRFLDSDGSAVDLIEASD
GFYRKTYHLVDGGLEAHALPLPAKAEIEAYIAGDFIVKLAEDWRWRGEAYPSGSLLAVGLPGLIGEGPAPQLLQAPAPRL
AVAAVETTRSRLLVNLLDNVKSRMLCFAKRDGAWETQPLALPADGVIEIADQPWQSDVLYYSYSDFLTPSGLYRLDLESG
DSECLRRQPEAFDPAPYVAEQWQATAPDGERIPYFVVRRRDAPFDGATPTLLYGYGGFEVPMLPYYIENFGPHWLAKGGA
FVLACIRGGGEFGPHWHQAAQGPKRQVSFDDFIAVAEDLIARKLTSPARLAIEGGSNGGLLVGAAMAQRPELFRAVVCEV
PLLDMLRYTQLLAGASWIDEYGDPDDEAERAALAAYSPYHNLRADARYPLALFTTSASDDRVHPGHARKMAARLLALGHD
ALFYETDGGGHAGNAGQEDTAAELARVLVYLYQRLCD
>Mature_677_residues
MNPSHDPHAWLESLDSDQAAAWVAEQNARTEGVLDADPRFAGLKADILAHLRDTRQIPYFAEHKGWLYNFHQDEAHPRGI
YRRTTLDAYRAGSQDWQTVLDIDALADADERDWYLDGVSHCTVRPERALVHLTDGGGDATVAREYDVETRRWVEDGFNFP
EGKNHIAWRDPDSAFVCPGWKGAPLTRAGYPREVWLVERAADGRHGWTLLFAAPEDAMMTAAWRFLDSDGSAVDLIEASD
GFYRKTYHLVDGGLEAHALPLPAKAEIEAYIAGDFIVKLAEDWRWRGEAYPSGSLLAVGLPGLIGEGPAPQLLQAPAPRL
AVAAVETTRSRLLVNLLDNVKSRMLCFAKRDGAWETQPLALPADGVIEIADQPWQSDVLYYSYSDFLTPSGLYRLDLESG
DSECLRRQPEAFDPAPYVAEQWQATAPDGERIPYFVVRRRDAPFDGATPTLLYGYGGFEVPMLPYYIENFGPHWLAKGGA
FVLACIRGGGEFGPHWHQAAQGPKRQVSFDDFIAVAEDLIARKLTSPARLAIEGGSNGGLLVGAAMAQRPELFRAVVCEV
PLLDMLRYTQLLAGASWIDEYGDPDDEAERAALAAYSPYHNLRADARYPLALFTTSASDDRVHPGHARKMAARLLALGHD
ALFYETDGGGHAGNAGQEDTAAELARVLVYLYQRLCD

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=318, Percent_Identity=35.5345911949685, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI284172438, Length=343, Percent_Identity=26.2390670553936, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI284172431, Length=343, Percent_Identity=26.2390670553936, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI284172420, Length=343, Percent_Identity=26.2390670553936, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI284172413, Length=343, Percent_Identity=26.2390670553936, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI70778815, Length=343, Percent_Identity=26.2390670553936, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI108860686, Length=209, Percent_Identity=30.622009569378, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI108860692, Length=204, Percent_Identity=30.8823529411765, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1788150, Length=289, Percent_Identity=32.5259515570934, Blast_Score=139, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24583414, Length=365, Percent_Identity=34.5205479452055, Blast_Score=203, Evalue=3e-52,
Organism=Drosophila melanogaster, GI221510989, Length=671, Percent_Identity=26.8256333830104, Blast_Score=178, Evalue=9e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 74851; Mature: 74851

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPSHDPHAWLESLDSDQAAAWVAEQNARTEGVLDADPRFAGLKADILAHLRDTRQIPYF
CCCCCCHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEE
AEHKGWLYNFHQDEAHPRGIYRRTTLDAYRAGSQDWQTVLDIDALADADERDWYLDGVSH
ECCCCEEEEECCCCCCCCCCEEHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEECCCCE
CTVRPERALVHLTDGGGDATVAREYDVETRRWVEDGFNFPEGKNHIAWRDPDSAFVCPGW
EEECCCCEEEEEECCCCCEEEEEECCCHHHHHHHHCCCCCCCCCEEEEECCCCCEECCCC
KGAPLTRAGYPREVWLVERAADGRHGWTLLFAAPEDAMMTAAWRFLDSDGSAVDLIEASD
CCCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCEEEEEECCC
GFYRKTYHLVDGGLEAHALPLPAKAEIEAYIAGDFIVKLAEDWRWRGEAYPSGSLLAVGL
CCEEEEEEEECCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCCEEEEEC
PGLIGEGPAPQLLQAPAPRLAVAAVETTRSRLLVNLLDNVKSRMLCFAKRDGAWETQPLA
CCCCCCCCCCHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEE
LPADGVIEIADQPWQSDVLYYSYSDFLTPSGLYRLDLESGDSECLRRQPEAFDPAPYVAE
CCCCCEEEECCCCCCCCEEEEEHHCCCCCCCEEEEECCCCCHHHHHCCCCCCCCCCHHHH
QWQATAPDGERIPYFVVRRRDAPFDGATPTLLYGYGGFEVPMLPYYIENFGPHWLAKGGA
CCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHCCCCHHHHCCCE
FVLACIRGGGEFGPHWHQAAQGPKRQVSFDDFIAVAEDLIARKLTSPARLAIEGGSNGGL
EEEEEEECCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCE
LVGAAMAQRPELFRAVVCEVPLLDMLRYTQLLAGASWIDEYGDPDDEAERAALAAYSPYH
EEEHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHCCCCH
NLRADARYPLALFTTSASDDRVHPGHARKMAARLLALGHDALFYETDGGGHAGNAGQEDT
HCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHH
AAELARVLVYLYQRLCD
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNPSHDPHAWLESLDSDQAAAWVAEQNARTEGVLDADPRFAGLKADILAHLRDTRQIPYF
CCCCCCHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEE
AEHKGWLYNFHQDEAHPRGIYRRTTLDAYRAGSQDWQTVLDIDALADADERDWYLDGVSH
ECCCCEEEEECCCCCCCCCCEEHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEECCCCE
CTVRPERALVHLTDGGGDATVAREYDVETRRWVEDGFNFPEGKNHIAWRDPDSAFVCPGW
EEECCCCEEEEEECCCCCEEEEEECCCHHHHHHHHCCCCCCCCCEEEEECCCCCEECCCC
KGAPLTRAGYPREVWLVERAADGRHGWTLLFAAPEDAMMTAAWRFLDSDGSAVDLIEASD
CCCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCEEEEEECCC
GFYRKTYHLVDGGLEAHALPLPAKAEIEAYIAGDFIVKLAEDWRWRGEAYPSGSLLAVGL
CCEEEEEEEECCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCCEEEEEC
PGLIGEGPAPQLLQAPAPRLAVAAVETTRSRLLVNLLDNVKSRMLCFAKRDGAWETQPLA
CCCCCCCCCCHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEE
LPADGVIEIADQPWQSDVLYYSYSDFLTPSGLYRLDLESGDSECLRRQPEAFDPAPYVAE
CCCCCEEEECCCCCCCCEEEEEHHCCCCCCCEEEEECCCCCHHHHHCCCCCCCCCCHHHH
QWQATAPDGERIPYFVVRRRDAPFDGATPTLLYGYGGFEVPMLPYYIENFGPHWLAKGGA
CCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHCCCCHHHHCCCE
FVLACIRGGGEFGPHWHQAAQGPKRQVSFDDFIAVAEDLIARKLTSPARLAIEGGSNGGL
EEEEEEECCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCE
LVGAAMAQRPELFRAVVCEVPLLDMLRYTQLLAGASWIDEYGDPDDEAERAALAAYSPYH
EEEHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHCCCCH
NLRADARYPLALFTTSASDDRVHPGHARKMAARLLALGHDALFYETDGGGHAGNAGQEDT
HCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHH
AAELARVLVYLYQRLCD
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.47 {tosyl-Lys-methyl} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]