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The map label for this gene is smf [H]

Identifier: 34499722

GI number: 34499722

Start: 4601094

End: 4602173

Strand: Direct

Name: smf [H]

Synonym: CV_4267

Alternate gene names: 34499722

Gene position: 4601094-4602173 (Clockwise)

Preceding gene: 34499721

Following gene: 34499723

Centisome position: 96.84

GC content: 74.26

Gene sequence:

>1080_bases
ATGAGCGGCGCCCCGCAAGCCTGGCTGACGCTGGCGCTGACCCCCGGCATCGGCCCGGTCGGCTTCCTGAAGCTGATAGA
CGCGTTCGGCTCGGCCGAGGCCGCGGCCGCCGCCCGTCCCGCCCAAACCGAGAAGCTGGTGGGCCGCGAGGCGGCCGAAG
CGCTGCAGGCCGGCGCGGCGGCGGAATCGGCCCAGGCCGCGCTCGCCTGGGCCGAAGGCGACGGCTGCCACTTGATCACG
CTGCTCGACGCCGACTACCCGGCGCAGCTGGCCGAAGCCGCGTCGCCGCCGCCGCTGCTGTTCGCGCGCGGCCGGCGCGA
GCTGCTGGCGCGGACCATGCTGGCCGTGGTCGGCAGCCGGGCTGCCACCCAGCAGGGCAAGCGCAACGCCGAGGACTTCG
CGTCGGCGCTGGCCGCCCACGGCTACACCATCGTCAGCGGCCTGGCCAGCGGCATCGACGCCGCCGCCCACCAGGGCGCG
CTCGCGCATCCGGCCTCCACCGTCGCGGTGATCGGCACCGGCATCGACCGCGTCTACCCGGCGTCCAACCGCCAGCTCGC
CCACCGCGTCGCCGAGCACGGGCTGATCCTGTCCGAGTTCCCGCTGGGCATGGGCCCGCTGCCCGGCCACTTCCCGCGCC
GCAACCGCATCATCGCCGGCCTAGCCCGCGGCTGCCTGGTGGTGGAGGCCGGCATCGCGTCCGGCTCGCTGATCAGCGCG
CGGCTGGCGATGGAGAACAACCGCGACGTGATGGCGATACCGGGCAGCATCCACAACGCCCAGGCGCGCGGCTGCCACCG
GCTGATCAAGGATGGCGCGCGGCTGGTGGAGAGCGTCGACGACGTGCTGGACGAGATCGGCCGGCTGCCGGCTGCGCCAC
CGCCGCCCCTCCGGCAAGAAGAGCCGGAAACGCCGCCGCTGTTGAACAGCATGGGTTGGGAGCCGGTGCTGGCGGACGCG
CTGGCCGGGACGCTCGGGTTGACTCCCGGGGAGGTTTACGCGATGCTGCTCGAACTGGAACTGGCCGGCCGCGTGGCCAG
CCTGCCGGGCGGCCGCTTCCAGCGGCTCGCACAACTCTGA

Upstream 100 bases:

>100_bases
TGTTCGTCTACCGCGTATTCGACAAGGTGTCCTACGCGCTGGTGCTGGACAGCCGGGGCGCGCTCTACGTCGGCGACAGC
GTCGCCACGCCGGAGAACGA

Downstream 100 bases:

>100_bases
ACGCCTGGCGTTCTTCACGAAAAATACCGATGTTTGACGTACTCACCTATCTATTCGAGCAATACAGCGACCCGGCCGCC
TTCGGCGACCGCAGCGCCCT

Product: smf protein

Products: NA

Alternate protein names: DNA-processing chain A [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MSGAPQAWLTLALTPGIGPVGFLKLIDAFGSAEAAAAARPAQTEKLVGREAAEALQAGAAAESAQAALAWAEGDGCHLIT
LLDADYPAQLAEAASPPPLLFARGRRELLARTMLAVVGSRAATQQGKRNAEDFASALAAHGYTIVSGLASGIDAAAHQGA
LAHPASTVAVIGTGIDRVYPASNRQLAHRVAEHGLILSEFPLGMGPLPGHFPRRNRIIAGLARGCLVVEAGIASGSLISA
RLAMENNRDVMAIPGSIHNAQARGCHRLIKDGARLVESVDDVLDEIGRLPAAPPPPLRQEEPETPPLLNSMGWEPVLADA
LAGTLGLTPGEVYAMLLELELAGRVASLPGGRFQRLAQL

Sequences:

>Translated_359_residues
MSGAPQAWLTLALTPGIGPVGFLKLIDAFGSAEAAAAARPAQTEKLVGREAAEALQAGAAAESAQAALAWAEGDGCHLIT
LLDADYPAQLAEAASPPPLLFARGRRELLARTMLAVVGSRAATQQGKRNAEDFASALAAHGYTIVSGLASGIDAAAHQGA
LAHPASTVAVIGTGIDRVYPASNRQLAHRVAEHGLILSEFPLGMGPLPGHFPRRNRIIAGLARGCLVVEAGIASGSLISA
RLAMENNRDVMAIPGSIHNAQARGCHRLIKDGARLVESVDDVLDEIGRLPAAPPPPLRQEEPETPPLLNSMGWEPVLADA
LAGTLGLTPGEVYAMLLELELAGRVASLPGGRFQRLAQL
>Mature_358_residues
SGAPQAWLTLALTPGIGPVGFLKLIDAFGSAEAAAAARPAQTEKLVGREAAEALQAGAAAESAQAALAWAEGDGCHLITL
LDADYPAQLAEAASPPPLLFARGRRELLARTMLAVVGSRAATQQGKRNAEDFASALAAHGYTIVSGLASGIDAAAHQGAL
AHPASTVAVIGTGIDRVYPASNRQLAHRVAEHGLILSEFPLGMGPLPGHFPRRNRIIAGLARGCLVVEAGIASGSLISAR
LAMENNRDVMAIPGSIHNAQARGCHRLIKDGARLVESVDDVLDEIGRLPAAPPPPLRQEEPETPPLLNSMGWEPVLADAL
AGTLGLTPGEVYAMLLELELAGRVASLPGGRFQRLAQL

Specific function: Essential for efficient chromosomal DNA transformation but not required for plasmid transformation [H]

COG id: COG0758

COG function: function code LU; Predicted Rossmann fold nucleotide-binding protein involved in DNA uptake

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smf family [H]

Homologues:

Organism=Escherichia coli, GI48994932, Length=300, Percent_Identity=42, Blast_Score=198, Evalue=4e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003488 [H]

Pfam domain/function: PF02481 DNA_processg_A [H]

EC number: NA

Molecular weight: Translated: 37104; Mature: 36973

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGAPQAWLTLALTPGIGPVGFLKLIDAFGSAEAAAAARPAQTEKLVGREAAEALQAGAA
CCCCCCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCH
AESAQAALAWAEGDGCHLITLLDADYPAQLAEAASPPPLLFARGRRELLARTMLAVVGSR
HHHHHHEEEEECCCCEEEEEEECCCCCHHHHHCCCCCCEEHHCCHHHHHHHHHHHHHHHH
AATQQGKRNAEDFASALAAHGYTIVSGLASGIDAAAHQGALAHPASTVAVIGTGIDRVYP
HHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHCCC
ASNRQLAHRVAEHGLILSEFPLGMGPLPGHFPRRNRIIAGLARGCLVVEAGIASGSLISA
CCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCHHHH
RLAMENNRDVMAIPGSIHNAQARGCHRLIKDGARLVESVDDVLDEIGRLPAAPPPPLRQE
HHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
EPETPPLLNSMGWEPVLADALAGTLGLTPGEVYAMLLELELAGRVASLPGGRFQRLAQL
CCCCCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHCCCCHHHHHHHCC
>Mature Secondary Structure 
SGAPQAWLTLALTPGIGPVGFLKLIDAFGSAEAAAAARPAQTEKLVGREAAEALQAGAA
CCCCCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCH
AESAQAALAWAEGDGCHLITLLDADYPAQLAEAASPPPLLFARGRRELLARTMLAVVGSR
HHHHHHEEEEECCCCEEEEEEECCCCCHHHHHCCCCCCEEHHCCHHHHHHHHHHHHHHHH
AATQQGKRNAEDFASALAAHGYTIVSGLASGIDAAAHQGALAHPASTVAVIGTGIDRVYP
HHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHCCC
ASNRQLAHRVAEHGLILSEFPLGMGPLPGHFPRRNRIIAGLARGCLVVEAGIASGSLISA
CCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCHHHH
RLAMENNRDVMAIPGSIHNAQARGCHRLIKDGARLVESVDDVLDEIGRLPAAPPPPLRQE
HHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
EPETPPLLNSMGWEPVLADALAGTLGLTPGEVYAMLLELELAGRVASLPGGRFQRLAQL
CCCCCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7768823; 7542800 [H]