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The map label for this gene is rsmB [H]

Identifier: 34499717

GI number: 34499717

Start: 4596218

End: 4597468

Strand: Reverse

Name: rsmB [H]

Synonym: CV_4262

Alternate gene names: 34499717

Gene position: 4597468-4596218 (Counterclockwise)

Preceding gene: 34499718

Following gene: 34499716

Centisome position: 96.77

GC content: 69.3

Gene sequence:

>1251_bases
ATGTATCAAATCCAGCAACTGGCCGCCGAGATCCTGGCGCGCATCGAGGCCGGCACCACCCTCACCGACGCGCTGGCCGA
CGTCCAGCGGCAGGCCGGCAAGCTGACGCCGGCCGAGCGCGGCGCGCTGCAGGACCTGAGCTACGGCAGCCTGCGCCATC
TGGGCGTATTGCGCCATTGCCTGCGCCAACTGGTGCCGCATCCGCTGCCGGAGCCGCAGGTGGAGCGCCTGCTGCTGATC
GCGCTGTACCAGCTGCAGTACACCCGCGCCGCGCAGTACGCGGTGGTGCACGAGGCGGTGACGCTGGCCGGCATGATGGC
GCGCGGCAAGTTCAAGGCGCTGGTCAACGGCGTGCTGCGCAACTTCCAGCGCCGCCGCGACGAACTGCTGGCCAATGCCG
CCGCCGATGAAGTGGCGCAGGCCAACCATCCCGACTGGTGGGTGGCGCGGCTGAAAGAAGCCTACCCCGCCGAATGGCGC
GCCATCCTGGAGGCCAGCAATGTCCATCCGCCGCTGACGCTGCGCGTCAATCGCCGCCGCATCTCGGTGGACGACTACCT
GCGCCGGCTGGAGGAGGCGGGCCTGGCGGCCAAGGCGCTGGGCGGTGACGCGGTGCAGCTGGCGCGGCCGGTGGCGGTGC
GCGACCTGCCCGGCTTTGCCGAAGGCCTGGCATCGGTGCAGGACGAGGGCGCGCAGCGCGCCGCGTACTGGCTGGACCTG
AAGCCCGGCATGCGGGTGCTGGATGCCTGCGCCGCGCCGGGCGGCAAGACCGGCCACATGCTGGAGATGGCCGACGTCGA
AGTCACCGCGCTGGACATCGACAACGCCCGTTTGGCCCGCGTCGGCGACAATCTGCGCCGCCTGGGCCTGTCCGCCCAGT
TGATCGAGGCCGACGCGTCGAGGCCGGACGAATGGTGGGGCGGCCGCCCCTTCGACCGCATCCTGGCCGATGTGCCTTGC
TCCGCCTCCGGCGTGGTGCGGCGCCATCCCGACATCAAGTGGCTGCGCCGGCCCGGCGATTTCGCGGCGCTGGGAGAGCA
GCAGGCGGGGATGGCCGATGCGCTGTGGCCGCTGCTCGCCAGCAGGGGAAAAATGCTATACGCTACCTGCTCGATTTTTC
CCGAAGAAAACCGCCTGCAGTTGGAAGGCTTCCTCAAGCGCCACCCGGATGCGTCGTGCCTGAAGGAGGAGCAACTGCTG
CCTTGTGAGCGACATGACGGCTTCTATTACGCGCTGCTTGAGAAACATTAG

Upstream 100 bases:

>100_bases
GCGGCTGCAGGCGCTGGCGCGCGGCGTCTACTAGCGCAGGCCCTTGAACCGACCCGCGGCCAGTCCCCCGGACTGGCCTT
GTTTTATCGAAAGACACACC

Downstream 100 bases:

>100_bases
CCTGTTGTTGGCTCTGCTTTGCTGCCTGCTGCCGCCGGCGCTGGCCGACACCATCGCGTCGCGCCGCGCCGAGGCGGAGG
TGATGCCGGACGGCACGCTG

Product: sun protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MYQIQQLAAEILARIEAGTTLTDALADVQRQAGKLTPAERGALQDLSYGSLRHLGVLRHCLRQLVPHPLPEPQVERLLLI
ALYQLQYTRAAQYAVVHEAVTLAGMMARGKFKALVNGVLRNFQRRRDELLANAAADEVAQANHPDWWVARLKEAYPAEWR
AILEASNVHPPLTLRVNRRRISVDDYLRRLEEAGLAAKALGGDAVQLARPVAVRDLPGFAEGLASVQDEGAQRAAYWLDL
KPGMRVLDACAAPGGKTGHMLEMADVEVTALDIDNARLARVGDNLRRLGLSAQLIEADASRPDEWWGGRPFDRILADVPC
SASGVVRRHPDIKWLRRPGDFAALGEQQAGMADALWPLLASRGKMLYATCSIFPEENRLQLEGFLKRHPDASCLKEEQLL
PCERHDGFYYALLEKH

Sequences:

>Translated_416_residues
MYQIQQLAAEILARIEAGTTLTDALADVQRQAGKLTPAERGALQDLSYGSLRHLGVLRHCLRQLVPHPLPEPQVERLLLI
ALYQLQYTRAAQYAVVHEAVTLAGMMARGKFKALVNGVLRNFQRRRDELLANAAADEVAQANHPDWWVARLKEAYPAEWR
AILEASNVHPPLTLRVNRRRISVDDYLRRLEEAGLAAKALGGDAVQLARPVAVRDLPGFAEGLASVQDEGAQRAAYWLDL
KPGMRVLDACAAPGGKTGHMLEMADVEVTALDIDNARLARVGDNLRRLGLSAQLIEADASRPDEWWGGRPFDRILADVPC
SASGVVRRHPDIKWLRRPGDFAALGEQQAGMADALWPLLASRGKMLYATCSIFPEENRLQLEGFLKRHPDASCLKEEQLL
PCERHDGFYYALLEKH
>Mature_416_residues
MYQIQQLAAEILARIEAGTTLTDALADVQRQAGKLTPAERGALQDLSYGSLRHLGVLRHCLRQLVPHPLPEPQVERLLLI
ALYQLQYTRAAQYAVVHEAVTLAGMMARGKFKALVNGVLRNFQRRRDELLANAAADEVAQANHPDWWVARLKEAYPAEWR
AILEASNVHPPLTLRVNRRRISVDDYLRRLEEAGLAAKALGGDAVQLARPVAVRDLPGFAEGLASVQDEGAQRAAYWLDL
KPGMRVLDACAAPGGKTGHMLEMADVEVTALDIDNARLARVGDNLRRLGLSAQLIEADASRPDEWWGGRPFDRILADVPC
SASGVVRRHPDIKWLRRPGDFAALGEQQAGMADALWPLLASRGKMLYATCSIFPEENRLQLEGFLKRHPDASCLKEEQLL
PCERHDGFYYALLEKH

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=289, Percent_Identity=29.0657439446367, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI76150623, Length=289, Percent_Identity=29.0657439446367, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI32698918, Length=228, Percent_Identity=30.2631578947368, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI270288816, Length=222, Percent_Identity=31.0810810810811, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI270288818, Length=222, Percent_Identity=31.0810810810811, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI8922322, Length=222, Percent_Identity=31.0810810810811, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI23199998, Length=184, Percent_Identity=33.1521739130435, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI39995082, Length=169, Percent_Identity=33.7278106508876, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI301336155, Length=169, Percent_Identity=33.7278106508876, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI2367212, Length=414, Percent_Identity=44.9275362318841, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI87081985, Length=311, Percent_Identity=26.3665594855305, Blast_Score=72, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17536757, Length=290, Percent_Identity=28.6206896551724, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI25143471, Length=147, Percent_Identity=37.4149659863946, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6324268, Length=291, Percent_Identity=27.1477663230241, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319447, Length=160, Percent_Identity=33.75, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI22024126, Length=312, Percent_Identity=26.6025641025641, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21355201, Length=169, Percent_Identity=32.5443786982249, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920494, Length=153, Percent_Identity=32.6797385620915, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 46222; Mature: 46222

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQIQQLAAEILARIEAGTTLTDALADVQRQAGKLTPAERGALQDLSYGSLRHLGVLRHC
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHCCCHHCCCCCHHHHHHHHHHH
LRQLVPHPLPEPQVERLLLIALYQLQYTRAAQYAVVHEAVTLAGMMARGKFKALVNGVLR
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NFQRRRDELLANAAADEVAQANHPDWWVARLKEAYPAEWRAILEASNVHPPLTLRVNRRR
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCE
ISVDDYLRRLEEAGLAAKALGGDAVQLARPVAVRDLPGFAEGLASVQDEGAQRAAYWLDL
ECHHHHHHHHHHCCCHHHHCCCCHHHHHCCHHHHCCCCHHHHHHHHHHCCCCCEEEEEEC
KPGMRVLDACAAPGGKTGHMLEMADVEVTALDIDNARLARVGDNLRRLGLSAQLIEADAS
CCCHHHHHHHCCCCCCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHCCCC
RPDEWWGGRPFDRILADVPCSASGVVRRHPDIKWLRRPGDFAALGEQQAGMADALWPLLA
CCCCCCCCCCHHHHHHCCCCCCCCCHHCCCCHHHHCCCCCHHHHCCHHCCHHHHHHHHHH
SRGKMLYATCSIFPEENRLQLEGFLKRHPDASCLKEEQLLPCERHDGFYYALLEKH
HCCCEEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MYQIQQLAAEILARIEAGTTLTDALADVQRQAGKLTPAERGALQDLSYGSLRHLGVLRHC
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHCCCHHCCCCCHHHHHHHHHHH
LRQLVPHPLPEPQVERLLLIALYQLQYTRAAQYAVVHEAVTLAGMMARGKFKALVNGVLR
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NFQRRRDELLANAAADEVAQANHPDWWVARLKEAYPAEWRAILEASNVHPPLTLRVNRRR
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCE
ISVDDYLRRLEEAGLAAKALGGDAVQLARPVAVRDLPGFAEGLASVQDEGAQRAAYWLDL
ECHHHHHHHHHHCCCHHHHCCCCHHHHHCCHHHHCCCCHHHHHHHHHHCCCCCEEEEEEC
KPGMRVLDACAAPGGKTGHMLEMADVEVTALDIDNARLARVGDNLRRLGLSAQLIEADAS
CCCHHHHHHHCCCCCCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHCCCC
RPDEWWGGRPFDRILADVPCSASGVVRRHPDIKWLRRPGDFAALGEQQAGMADALWPLLA
CCCCCCCCCCHHHHHHCCCCCCCCCHHCCCCHHHHCCCCCHHHHCCHHCCHHHHHHHHHH
SRGKMLYATCSIFPEENRLQLEGFLKRHPDASCLKEEQLLPCERHDGFYYALLEKH
HCCCEEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA