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The map label for this gene is exoA [H]

Identifier: 34499702

GI number: 34499702

Start: 4584103

End: 4584879

Strand: Reverse

Name: exoA [H]

Synonym: CV_4247

Alternate gene names: 34499702

Gene position: 4584879-4584103 (Counterclockwise)

Preceding gene: 34499705

Following gene: 34499701

Centisome position: 96.5

GC content: 64.22

Gene sequence:

>777_bases
ATGCTTCGAATCGTTTCCGCCAATCTCAACGGCATCCGCTCGGCGGACAAGAAAGGCTTTTTCGACTGGCTGGCCGGCAT
CGACGCCGACTTCGTCTGCGTGCAGGAGCTGAAGGCGCAGGCGGGCGACCTGTCCGAGCGGATGCGCAATCCGGACGGCC
TGGCCGGCTACTTCCACTACGCCGAGAAGAAGGGCTACAGCGGAGTAGGCCTCTACACCCGGCACCAGCCGGACGCGGTA
GTGGAAGGGTTGGGCGTGGACTGGATCGACGCCGAGGGCCGCTTTCTGCAGCTGGACTTCGGCAAGCTGTCGGTGGTGTC
GCTGTATCTGCCGTCCGGCTCCAGCAGCGACGAGCGCCAGCAAGTGAAGTTCGACTTCCTCGACGTGTTCATGCCGCACC
TGGAAAAACTGCGCGCCGAAGGCCGCGACATCGTGATCTGCGGCGACTGGAACATCGCCCACAACGAGATCGACCTGAAG
AACTGGAAGGGCAATCTGAAGAACTCCGGCTTCCTGCCGGAGGAGCGCGCCTGGATGACCGATCTGTTGGGCCGCGTCGG
CTGGCGCGACGTGTGGCGCAATCTGTACCCGGAAGCGCCGGGCTACACCTGGTGGAGCAACCGCGGCCAGGCCTACGCCA
AGGACGTCGGCTGGCGCATCGACTACCAGATCGTCACGCCGTCGCTGATGGAGCTGGCCGAGTCCGCCAGCGTGTACAAG
GACGAGAAATTCTCCGACCACGCGCCGCTGATCGTCGACTACCGGCGGAGCCTGTGA

Upstream 100 bases:

>100_bases
ATAAAATCCTGACGAAAGTCGCTCATCCTCATTCCTTATCGAATCGTTAACTGTTTGAACGGCTGAAAAAAACCGCCGCA
AGCATAGCACGGGAGACACA

Downstream 100 bases:

>100_bases
TGTGGGACGACGATGACGTCACGCTGCTGACGGTGCCCGGCTGGGGCAATTCCGGCGACACGCACTGGCACGGCTACTGG
GAAAAAACCTATCCGCTGGC

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLRIVSANLNGIRSADKKGFFDWLAGIDADFVCVQELKAQAGDLSERMRNPDGLAGYFHYAEKKGYSGVGLYTRHQPDAV
VEGLGVDWIDAEGRFLQLDFGKLSVVSLYLPSGSSSDERQQVKFDFLDVFMPHLEKLRAEGRDIVICGDWNIAHNEIDLK
NWKGNLKNSGFLPEERAWMTDLLGRVGWRDVWRNLYPEAPGYTWWSNRGQAYAKDVGWRIDYQIVTPSLMELAESASVYK
DEKFSDHAPLIVDYRRSL

Sequences:

>Translated_258_residues
MLRIVSANLNGIRSADKKGFFDWLAGIDADFVCVQELKAQAGDLSERMRNPDGLAGYFHYAEKKGYSGVGLYTRHQPDAV
VEGLGVDWIDAEGRFLQLDFGKLSVVSLYLPSGSSSDERQQVKFDFLDVFMPHLEKLRAEGRDIVICGDWNIAHNEIDLK
NWKGNLKNSGFLPEERAWMTDLLGRVGWRDVWRNLYPEAPGYTWWSNRGQAYAKDVGWRIDYQIVTPSLMELAESASVYK
DEKFSDHAPLIVDYRRSL
>Mature_258_residues
MLRIVSANLNGIRSADKKGFFDWLAGIDADFVCVQELKAQAGDLSERMRNPDGLAGYFHYAEKKGYSGVGLYTRHQPDAV
VEGLGVDWIDAEGRFLQLDFGKLSVVSLYLPSGSSSDERQQVKFDFLDVFMPHLEKLRAEGRDIVICGDWNIAHNEIDLK
NWKGNLKNSGFLPEERAWMTDLLGRVGWRDVWRNLYPEAPGYTWWSNRGQAYAKDVGWRIDYQIVTPSLMELAESASVYK
DEKFSDHAPLIVDYRRSL

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=255, Percent_Identity=36.4705882352941, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI18375503, Length=255, Percent_Identity=36.4705882352941, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI18375501, Length=255, Percent_Identity=36.4705882352941, Blast_Score=162, Evalue=3e-40,
Organism=Escherichia coli, GI1788046, Length=272, Percent_Identity=28.3088235294118, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71989536, Length=253, Percent_Identity=28.8537549407115, Blast_Score=124, Evalue=3e-29,
Organism=Drosophila melanogaster, GI221330655, Length=254, Percent_Identity=33.8582677165354, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI17136678, Length=254, Percent_Identity=33.8582677165354, Blast_Score=139, Evalue=2e-33,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29434; Mature: 29434

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRIVSANLNGIRSADKKGFFDWLAGIDADFVCVQELKAQAGDLSERMRNPDGLAGYFHY
CEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHCCCCCCHHHHHH
AEKKGYSGVGLYTRHQPDAVVEGLGVDWIDAEGRFLQLDFGKLSVVSLYLPSGSSSDERQ
HHHCCCCCCCEEECCCCHHHHHCCCCEEECCCCCEEEEECCCEEEEEEEECCCCCCCHHH
QVKFDFLDVFMPHLEKLRAEGRDIVICGDWNIAHNEIDLKNWKGNLKNSGFLPEERAWMT
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEECCEEECCCCCCCCCCCCCCCHHHHHHH
DLLGRVGWRDVWRNLYPEAPGYTWWSNRGQAYAKDVGWRIDYQIVTPSLMELAESASVYK
HHHHHCCHHHHHHHHCCCCCCCCEECCCCCEEHHCCCCEEEEEEECHHHHHHHHHHHHHC
DEKFSDHAPLIVDYRRSL
CCCCCCCCCEEEECCCCC
>Mature Secondary Structure
MLRIVSANLNGIRSADKKGFFDWLAGIDADFVCVQELKAQAGDLSERMRNPDGLAGYFHY
CEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHCCCCCCHHHHHH
AEKKGYSGVGLYTRHQPDAVVEGLGVDWIDAEGRFLQLDFGKLSVVSLYLPSGSSSDERQ
HHHCCCCCCCEEECCCCHHHHHCCCCEEECCCCCEEEEECCCEEEEEEEECCCCCCCHHH
QVKFDFLDVFMPHLEKLRAEGRDIVICGDWNIAHNEIDLKNWKGNLKNSGFLPEERAWMT
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEECCEEECCCCCCCCCCCCCCCHHHHHHH
DLLGRVGWRDVWRNLYPEAPGYTWWSNRGQAYAKDVGWRIDYQIVTPSLMELAESASVYK
HHHHHCCHHHHHHHHCCCCCCCCEECCCCCEEHHCCCCEEEEEEECHHHHHHHHHHHHHC
DEKFSDHAPLIVDYRRSL
CCCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]