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The map label for this gene is pleD [H]

Identifier: 34499692

GI number: 34499692

Start: 4574131

End: 4575603

Strand: Direct

Name: pleD [H]

Synonym: CV_4237

Alternate gene names: 34499692

Gene position: 4574131-4575603 (Clockwise)

Preceding gene: 34499691

Following gene: 34499693

Centisome position: 96.28

GC content: 66.26

Gene sequence:

>1473_bases
GTGTCCGGCCCATTTTCTCGCTATCGCCTGGCCATCATCGTCGCCCTGCTGTTGCTTGCCGGCATCTTCGTCAATGCCGG
CCTGTACTACCGGCAGGGCAGTCAGGACATCCGCCACGGCATTGTCGAGCAAGGGCTGCCGCTGACCGGCGACACCATTT
TTTCCGAAATAGACAAAAGCATCCTGCGCCCGCTGTCCGTCGCCGCGCTGATGGCGAACGACACCTTCCTGCAGGACTGG
CTGAAAGACGGCGAACGCGACCCCGAACGCGTCGCCCGCTACTTGGCCGCCATCAACGGCCGCTACGGCATCACCGGCAG
CTTCCTGGTTTCGGAAAACAGCCGCCGCTACTATTACGCCGGCGGCGTGCTGAAAACCGTGTCGCCCGCCGACCGCGAGG
ATGTCTGGTACTTCCATAGCCGCCAGATCAAGGAAGCCTACGACATCACCCTGGACGAGGACATGACCAACGGCGGCAAG
ACCACGTTGTTCATCAACCACCGGATGCTGGACGGCAAAGGCAAGCTGCTGGGCGTGATCGGCGTCGGTCTGCCGCAGCT
GGAGCTGAGCGCGCAGCTGGCCGCGCACGAACAACGCTTCCAGCGCCGCATCTACTTCATCGACCGGAGCGGGCGCATCG
TGCTGGACAGCTCGGGCACCACGCCCCGCAGGCTGGCCGAGCAGCCCGGCCTGGCGAACCTGGCCTCCGCCATCCTTGCC
GGCGGTACTGAGCCCAAAAAACTGGAATACAGCCGCGACGGCAACCGCTACCTGCTGACCAGCCGCCTGCTGCCGGAGCT
GGGCTGGCACCTGCTGGTGGAACAGAACGAAACCCTGGCGCTGCAGCCGCTGCACGCCGCGCTGCAGGTGAACCTGGCCA
TCAACACCACCATCCTGCTGCTGGTGCTGATCGCCGTGCTGGCCACCTTGCAGCATTACCAGCGGCGGATGCGGCAACTG
GCCACCACCGATCCGCTGACCGGATGCCTGAACCGGCTGGCCTTTCTCGACCTGCTGCCCGGCTGGCTGCGCGCCTGCGA
GCGCACCGGCGCCGAACCCCAGCTGCTGATGATAGACATCGACCACTTCAAGCAGGTCAACGACAAGCACGGCCACCAGA
TCGGCGATCAGGTACTGCGCGAAATCGCCGACCTGCTGCGCTCGCAGCTGCGCGGCAGCGACCTGCTGATCCGCTGGGGA
GGAGAGGAATTCGTGGTGCTGGTGGGCAGCCATGGCGAAGACGACGCGCTGGCCGAGCGCCTGCGCACCGCCGTCGCCCG
CCACGTGATGAGCGCCTCCGGCAAGCGGCTGACCGTGACCGTCAGCCTGGGCCTGTGCCAATGGCTGCCGCCGGAGACGC
TGGAAGCCGCGGTCGCCCGCGCCGATCTGGCCTTGTATGCCGCCAAGCAGGGCGGCCGCAACCGCGTCTGCCACGCCGCG
CCCTATCAGCCGATAGACGAGCCGATCGCATGA

Upstream 100 bases:

>100_bases
CGGGCCGCGACCCATTCATTCCTGTTCACAGCTGACGTAGACGCTCGAAACGCCTATCTTCATATTAGAATTCGCGCTTT
CGTCACTCTGAAGGGTAGCC

Downstream 100 bases:

>100_bases
TAAATGATTACAATATGGACTTTGACCGTAAAGTCCACCCATGCCTCCCATCCAGCTGCTGATGCGCGACGATTGCTGGA
ACATCGGCCCGGAGCTGGCC

Product: GGDEF family protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MSGPFSRYRLAIIVALLLLAGIFVNAGLYYRQGSQDIRHGIVEQGLPLTGDTIFSEIDKSILRPLSVAALMANDTFLQDW
LKDGERDPERVARYLAAINGRYGITGSFLVSENSRRYYYAGGVLKTVSPADREDVWYFHSRQIKEAYDITLDEDMTNGGK
TTLFINHRMLDGKGKLLGVIGVGLPQLELSAQLAAHEQRFQRRIYFIDRSGRIVLDSSGTTPRRLAEQPGLANLASAILA
GGTEPKKLEYSRDGNRYLLTSRLLPELGWHLLVEQNETLALQPLHAALQVNLAINTTILLLVLIAVLATLQHYQRRMRQL
ATTDPLTGCLNRLAFLDLLPGWLRACERTGAEPQLLMIDIDHFKQVNDKHGHQIGDQVLREIADLLRSQLRGSDLLIRWG
GEEFVVLVGSHGEDDALAERLRTAVARHVMSASGKRLTVTVSLGLCQWLPPETLEAAVARADLALYAAKQGGRNRVCHAA
PYQPIDEPIA

Sequences:

>Translated_490_residues
MSGPFSRYRLAIIVALLLLAGIFVNAGLYYRQGSQDIRHGIVEQGLPLTGDTIFSEIDKSILRPLSVAALMANDTFLQDW
LKDGERDPERVARYLAAINGRYGITGSFLVSENSRRYYYAGGVLKTVSPADREDVWYFHSRQIKEAYDITLDEDMTNGGK
TTLFINHRMLDGKGKLLGVIGVGLPQLELSAQLAAHEQRFQRRIYFIDRSGRIVLDSSGTTPRRLAEQPGLANLASAILA
GGTEPKKLEYSRDGNRYLLTSRLLPELGWHLLVEQNETLALQPLHAALQVNLAINTTILLLVLIAVLATLQHYQRRMRQL
ATTDPLTGCLNRLAFLDLLPGWLRACERTGAEPQLLMIDIDHFKQVNDKHGHQIGDQVLREIADLLRSQLRGSDLLIRWG
GEEFVVLVGSHGEDDALAERLRTAVARHVMSASGKRLTVTVSLGLCQWLPPETLEAAVARADLALYAAKQGGRNRVCHAA
PYQPIDEPIA
>Mature_489_residues
SGPFSRYRLAIIVALLLLAGIFVNAGLYYRQGSQDIRHGIVEQGLPLTGDTIFSEIDKSILRPLSVAALMANDTFLQDWL
KDGERDPERVARYLAAINGRYGITGSFLVSENSRRYYYAGGVLKTVSPADREDVWYFHSRQIKEAYDITLDEDMTNGGKT
TLFINHRMLDGKGKLLGVIGVGLPQLELSAQLAAHEQRFQRRIYFIDRSGRIVLDSSGTTPRRLAEQPGLANLASAILAG
GTEPKKLEYSRDGNRYLLTSRLLPELGWHLLVEQNETLALQPLHAALQVNLAINTTILLLVLIAVLATLQHYQRRMRQLA
TTDPLTGCLNRLAFLDLLPGWLRACERTGAEPQLLMIDIDHFKQVNDKHGHQIGDQVLREIADLLRSQLRGSDLLIRWGG
EEFVVLVGSHGEDDALAERLRTAVARHVMSASGKRLTVTVSLGLCQWLPPETLEAAVARADLALYAAKQGGRNRVCHAAP
YQPIDEPIA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=184, Percent_Identity=37.5, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI145693134, Length=167, Percent_Identity=33.5329341317365, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1786584, Length=173, Percent_Identity=34.6820809248555, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1787816, Length=182, Percent_Identity=36.2637362637363, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787262, Length=189, Percent_Identity=35.4497354497354, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788381, Length=187, Percent_Identity=36.3636363636364, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082007, Length=200, Percent_Identity=34.5, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787541, Length=280, Percent_Identity=28.2142857142857, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787802, Length=128, Percent_Identity=35.9375, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87081974, Length=178, Percent_Identity=30.8988764044944, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788085, Length=165, Percent_Identity=29.6969696969697, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787056, Length=166, Percent_Identity=31.3253012048193, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI87081977, Length=163, Percent_Identity=28.8343558282209, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 54423; Mature: 54292

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGPFSRYRLAIIVALLLLAGIFVNAGLYYRQGSQDIRHGIVEQGLPLTGDTIFSEIDKS
CCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHH
ILRPLSVAALMANDTFLQDWLKDGERDPERVARYLAAINGRYGITGSFLVSENSRRYYYA
HHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEE
GGVLKTVSPADREDVWYFHSRQIKEAYDITLDEDMTNGGKTTLFINHRMLDGKGKLLGVI
CCEEECCCCCCCCCEEEHHHHHHHHHHCCEECCCCCCCCCEEEEEEEEEECCCCCEEEEE
GVGLPQLELSAQLAAHEQRFQRRIYFIDRSGRIVLDSSGTTPRRLAEQPGLANLASAILA
ECCCCCHHHHHHHHHHHHHHCCEEEEEECCCEEEEECCCCCHHHHHCCCCHHHHHHHHHC
GGTEPKKLEYSRDGNRYLLTSRLLPELGWHLLVEQNETLALQPLHAALQVNLAINTTILL
CCCCCCEEEECCCCCEEEEHHHHHHHCCCEEEEECCCEEEEEEEEEEEEEEEHHHHHHHH
LVLIAVLATLQHYQRRMRQLATTDPLTGCLNRLAFLDLLPGWLRACERTGAEPQLLMIDI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEH
DHFKQVNDKHGHQIGDQVLREIADLLRSQLRGSDLLIRWGGEEFVVLVGSHGEDDALAER
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCHHHHHHH
LRTAVARHVMSASGKRLTVTVSLGLCQWLPPETLEAAVARADLALYAAKQGGRNRVCHAA
HHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECC
PYQPIDEPIA
CCCCCCCCCC
>Mature Secondary Structure 
SGPFSRYRLAIIVALLLLAGIFVNAGLYYRQGSQDIRHGIVEQGLPLTGDTIFSEIDKS
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHH
ILRPLSVAALMANDTFLQDWLKDGERDPERVARYLAAINGRYGITGSFLVSENSRRYYYA
HHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEE
GGVLKTVSPADREDVWYFHSRQIKEAYDITLDEDMTNGGKTTLFINHRMLDGKGKLLGVI
CCEEECCCCCCCCCEEEHHHHHHHHHHCCEECCCCCCCCCEEEEEEEEEECCCCCEEEEE
GVGLPQLELSAQLAAHEQRFQRRIYFIDRSGRIVLDSSGTTPRRLAEQPGLANLASAILA
ECCCCCHHHHHHHHHHHHHHCCEEEEEECCCEEEEECCCCCHHHHHCCCCHHHHHHHHHC
GGTEPKKLEYSRDGNRYLLTSRLLPELGWHLLVEQNETLALQPLHAALQVNLAINTTILL
CCCCCCEEEECCCCCEEEEHHHHHHHCCCEEEEECCCEEEEEEEEEEEEEEEHHHHHHHH
LVLIAVLATLQHYQRRMRQLATTDPLTGCLNRLAFLDLLPGWLRACERTGAEPQLLMIDI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEH
DHFKQVNDKHGHQIGDQVLREIADLLRSQLRGSDLLIRWGGEEFVVLVGSHGEDDALAER
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCHHHHHHH
LRTAVARHVMSASGKRLTVTVSLGLCQWLPPETLEAAVARADLALYAAKQGGRNRVCHAA
HHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECC
PYQPIDEPIA
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA