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The map label for this gene is yfhA [H]

Identifier: 34499675

GI number: 34499675

Start: 4554607

End: 4555947

Strand: Direct

Name: yfhA [H]

Synonym: CV_4220

Alternate gene names: 34499675

Gene position: 4554607-4555947 (Clockwise)

Preceding gene: 34499674

Following gene: 34499676

Centisome position: 95.86

GC content: 71.74

Gene sequence:

>1341_bases
ATGAACGCCCCCGCCCGCATCGTCCTGGTGGACGACGATCCCGATCTGCTGTGCCTGGTCCGGCTGCGGCTTGAGGCCGC
CGGCCACGAAGTGGAGGCGCTCGCCAGCGCCGAGGCCGCGCTGGCAGCGCTGGCCGCGCGCCGCGCCGACCTGTTGCTGA
CCGACTGGCGCTTGCCCGGCATGGACGGGCTGCAGCTGTTCGAAACCGCGCGCCGCCGCTATCCGACGCTGCCGGTCATC
CTGTTCACCGCCCACGGCACGGTGCCGGACGCGGTGGAGGCGGTCAGCCGCGGCGTGTTCTGCTACCTGGAAAAACCGTT
CGACGGCCAGCTGCTGCTGGACAAGGTGGCCCAGGGACTGGCGCTGTCCGCGGGCGACGGCGCCGCCGGCGACGACTGGC
GCGCCGGCATCGTCAGCCGGGGCCCGGCGATGGAGGAACTGCTGAGCGAAGCGCGCATGGTGGCCGCCACCGACGCCAGC
GTGCTGCTGCAGGGCGAAAGCGGCAGCGGCAAGGAAGTGCTGGCGCGCGCCATTCATCAGGCCAGCCGCCGCGCTGGCCG
GCCCTTTGTCGCCGTCAACTGCGGCGCGATCCCGGAAAACCTGCTGGAAAGCGAGCTGTTCGGCCACGAGAAGGGCGCCT
TCACCGGCGCCGCCGCGCGCCACGACGGCCTGATCCGCCAGGCGGACGGCGGCACGCTGTTCCTGGACGAGATCGGCGAC
ATGCCGCTGCCGCTGCAGGTGAAGCTGCTGCGGGTGCTGCAGGATCGCGAGGTCAGGCCGGTGGGCGCCAGCCGGCCGCT
GCCGGTGGATATCCGGCTGCTGTCGGCCACCCACCGCGATTTGCCGGCCCGCATCGCCGACGGCCAGTTCCGCGAGGACC
TCTACTATCGGCTGAACGTGGTGTCGCTGACCCTGCCGCCCTTGGCCGAGCGGCGCGAGGACATCCCCTTGCTGGCGCAG
CATTTCCTGCAGCAGGTGGCCGCGCGCTACGGCAAGTCCGTCAGCGGCTTCGCGCCCGACGCCCTGGCCTTCCTCGCCGC
CGCGCGCTGGCCGGGCAATATCCGCCAGCTGGCCAATGTGGTGGAGCAATGCTGCGTGCTGGCGACCGGCCCCTTGCTGA
CCCTGGCCCAGGTGGAAAAGGCGGTAGCCGGCGAGTCGCGCGCGATTCCGACGCTGGCCGAGGCGCGGCGGGACTTCGAA
CGCGACTACCTGGAGCGGCTGCTGCGCCTGACCGGCGGCAACGTCAGCGACGCCGCCCGGCTGGCCGACCGCAATCGCAC
CGAGTTCTACCGGCTGCTGCAGAAACACGAGCTGAATGCCGCCGCGTTCAAGGACGGCTGA

Upstream 100 bases:

>100_bases
AGCAAAAGCGCATCGACGACCAGGCCGCCAAGCTGGAGGCGCTGCGCCGGCTGGAGCTGGACCTGCCCAAGCCCAACGGC
CTGAATGGAGGGAGCAAGCG

Downstream 100 bases:

>100_bases
GCCTGTCGCCGAACAGCGACAGGCCCAAGCCATTGATATGACTGCCCTTTTTCCGCATCGCCATCTCCGCTGTCGCCTGC
AGGCGACAAAATCCGGATGG

Product: two-component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MNAPARIVLVDDDPDLLCLVRLRLEAAGHEVEALASAEAALAALAARRADLLLTDWRLPGMDGLQLFETARRRYPTLPVI
LFTAHGTVPDAVEAVSRGVFCYLEKPFDGQLLLDKVAQGLALSAGDGAAGDDWRAGIVSRGPAMEELLSEARMVAATDAS
VLLQGESGSGKEVLARAIHQASRRAGRPFVAVNCGAIPENLLESELFGHEKGAFTGAAARHDGLIRQADGGTLFLDEIGD
MPLPLQVKLLRVLQDREVRPVGASRPLPVDIRLLSATHRDLPARIADGQFREDLYYRLNVVSLTLPPLAERREDIPLLAQ
HFLQQVAARYGKSVSGFAPDALAFLAAARWPGNIRQLANVVEQCCVLATGPLLTLAQVEKAVAGESRAIPTLAEARRDFE
RDYLERLLRLTGGNVSDAARLADRNRTEFYRLLQKHELNAAAFKDG

Sequences:

>Translated_446_residues
MNAPARIVLVDDDPDLLCLVRLRLEAAGHEVEALASAEAALAALAARRADLLLTDWRLPGMDGLQLFETARRRYPTLPVI
LFTAHGTVPDAVEAVSRGVFCYLEKPFDGQLLLDKVAQGLALSAGDGAAGDDWRAGIVSRGPAMEELLSEARMVAATDAS
VLLQGESGSGKEVLARAIHQASRRAGRPFVAVNCGAIPENLLESELFGHEKGAFTGAAARHDGLIRQADGGTLFLDEIGD
MPLPLQVKLLRVLQDREVRPVGASRPLPVDIRLLSATHRDLPARIADGQFREDLYYRLNVVSLTLPPLAERREDIPLLAQ
HFLQQVAARYGKSVSGFAPDALAFLAAARWPGNIRQLANVVEQCCVLATGPLLTLAQVEKAVAGESRAIPTLAEARRDFE
RDYLERLLRLTGGNVSDAARLADRNRTEFYRLLQKHELNAAAFKDG
>Mature_446_residues
MNAPARIVLVDDDPDLLCLVRLRLEAAGHEVEALASAEAALAALAARRADLLLTDWRLPGMDGLQLFETARRRYPTLPVI
LFTAHGTVPDAVEAVSRGVFCYLEKPFDGQLLLDKVAQGLALSAGDGAAGDDWRAGIVSRGPAMEELLSEARMVAATDAS
VLLQGESGSGKEVLARAIHQASRRAGRPFVAVNCGAIPENLLESELFGHEKGAFTGAAARHDGLIRQADGGTLFLDEIGD
MPLPLQVKLLRVLQDREVRPVGASRPLPVDIRLLSATHRDLPARIADGQFREDLYYRLNVVSLTLPPLAERREDIPLLAQ
HFLQQVAARYGKSVSGFAPDALAFLAAARWPGNIRQLANVVEQCCVLATGPLLTLAQVEKAVAGESRAIPTLAEARRDFE
RDYLERLLRLTGGNVSDAARLADRNRTEFYRLLQKHELNAAAFKDG

Specific function: Probable member of a two-component regulatory system yfhA/yfhK [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=444, Percent_Identity=54.954954954955, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI1790437, Length=423, Percent_Identity=45.6264775413712, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI1788550, Length=459, Percent_Identity=42.0479302832244, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI1790299, Length=460, Percent_Identity=41.0869565217391, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI87082117, Length=258, Percent_Identity=53.4883720930233, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1789233, Length=232, Percent_Identity=55.6034482758621, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1789087, Length=261, Percent_Identity=48.2758620689655, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI87082152, Length=315, Percent_Identity=41.2698412698413, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI87081872, Length=323, Percent_Identity=40.2476780185758, Blast_Score=214, Evalue=6e-57,
Organism=Escherichia coli, GI1786524, Length=244, Percent_Identity=47.1311475409836, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI1787583, Length=297, Percent_Identity=38.3838383838384, Blast_Score=191, Evalue=8e-50,
Organism=Escherichia coli, GI1789828, Length=259, Percent_Identity=38.996138996139, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI87081858, Length=310, Percent_Identity=29.6774193548387, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48356; Mature: 48356

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPARIVLVDDDPDLLCLVRLRLEAAGHEVEALASAEAALAALAARRADLLLTDWRLPG
CCCCEEEEEEECCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCC
MDGLQLFETARRRYPTLPVILFTAHGTVPDAVEAVSRGVFCYLEKPFDGQLLLDKVAQGL
CCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCEEEEEECCCCCHHHHHHHHCCC
ALSAGDGAAGDDWRAGIVSRGPAMEELLSEARMVAATDASVLLQGESGSGKEVLARAIHQ
EEECCCCCCCCCHHCCHHCCCHHHHHHHHHHHHEEECCCEEEEECCCCCCHHHHHHHHHH
ASRRAGRPFVAVNCGAIPENLLESELFGHEKGAFTGAAARHDGLIRQADGGTLFLDEIGD
HHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHCCCEEECCCCEEEHHHHCC
MPLPLQVKLLRVLQDREVRPVGASRPLPVDIRLLSATHRDLPARIADGQFREDLYYRLNV
CCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECHHCCCCCCHHCCCHHHHHHHEEEEE
VSLTLPPLAERREDIPLLAQHFLQQVAARYGKSVSGFAPDALAFLAAARWPGNIRQLANV
EEEECCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHH
VEQCCVLATGPLLTLAQVEKAVAGESRAIPTLAEARRDFERDYLERLLRLTGGNVSDAAR
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LADRNRTEFYRLLQKHELNAAAFKDG
HHCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNAPARIVLVDDDPDLLCLVRLRLEAAGHEVEALASAEAALAALAARRADLLLTDWRLPG
CCCCEEEEEEECCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCC
MDGLQLFETARRRYPTLPVILFTAHGTVPDAVEAVSRGVFCYLEKPFDGQLLLDKVAQGL
CCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCEEEEEECCCCCHHHHHHHHCCC
ALSAGDGAAGDDWRAGIVSRGPAMEELLSEARMVAATDASVLLQGESGSGKEVLARAIHQ
EEECCCCCCCCCHHCCHHCCCHHHHHHHHHHHHEEECCCEEEEECCCCCCHHHHHHHHHH
ASRRAGRPFVAVNCGAIPENLLESELFGHEKGAFTGAAARHDGLIRQADGGTLFLDEIGD
HHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHCCCEEECCCCEEEHHHHCC
MPLPLQVKLLRVLQDREVRPVGASRPLPVDIRLLSATHRDLPARIADGQFREDLYYRLNV
CCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECHHCCCCCCHHCCCHHHHHHHEEEEE
VSLTLPPLAERREDIPLLAQHFLQQVAARYGKSVSGFAPDALAFLAAARWPGNIRQLANV
EEEECCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHH
VEQCCVLATGPLLTLAQVEKAVAGESRAIPTLAEARRDFERDYLERLLRLTGGNVSDAAR
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LADRNRTEFYRLLQKHELNAAAFKDG
HHCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]