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The map label for this gene is potF1 [H]

Identifier: 34499668

GI number: 34499668

Start: 4547613

End: 4548686

Strand: Direct

Name: potF1 [H]

Synonym: CV_4213

Alternate gene names: 34499668

Gene position: 4547613-4548686 (Clockwise)

Preceding gene: 34499667

Following gene: 34499671

Centisome position: 95.72

GC content: 62.76

Gene sequence:

>1074_bases
ATGAACAAGATCGCCCTGGCCGCCTTGTTGGCGGTGGCCTGCGGTTCGGCCGCAGCCGAAGACGTGCTGCACATCTACAA
CTGGAACAACGCGCTGTCGCCTGACACCGCCAAGCGCTTCGAGCAGTCGTGCAAGTGCAAGCTGGTGCAGGACTACTACG
GCGACAACGAAGAGATGCTGGCCAAGCTGGCCGCCGGAGCCAAGGGCTACGACATGGTGTTTCCCACCGCTTTCGCGGTG
AACACGCTGCTGAAACAGGGCAAGCTGCAGCCGCTGGACAAGAGCAGGCTGCCCAACTGGAAGAACCTGAACAGCGGCTA
TCTGGCGCTGAACCAGCCCTTCGACCCCGGCAACCGCTACGCCGCGCCGACCGTGGTGTCGCTGACGCTGATCGGCTACA
ACGCCACCCAGCTGAAGAAGGCCGGCGTCGAGGGCAAGTCCAACAGCTGGGCGCTGGTATTCGACCCCGCGCTGCTGGCC
AAGATCAAGGGCAAGGTCACCGTGCTGGACAGCCAGCGCGAACTGATGGCCGCCGCGCTGATGTATCTGGGCAAGGACGC
CAACTCCACCAACCCGGCCGACTGGAAGGCCGCGGCGGAAACCATACGCAAGGCCAAGCCGTACTGGGCGGCGTTCAACA
ACCAGAGCTACATCAAGGAGCTGACCGTCGGCAACATCTGGGTGGCGCTGGGCTATTCCAACGACCTTTTCCAGGCCCAG
CAGGACGCGAAGAACGCCAAGCGGCCGTTCGAGCTCGCCTACCGCCCGCAGCAGGAAGGCAATGTGCTGGCCATCGACAA
CATGACCATCCTGAAGGACGCGCCGCGGCCGGACCTGGCGCACAAGTTCATCAACTTCATGCTGGACGGCAAAAACGCGT
CGGAAATCTCCAACCAGATCGGCGCGACCAATCCGGTGAAGGCGGCCGAAGCCTTCTTCAAGCCGCAGATCAAGGCCAAT
CCGGTGATCATGCTGGATCCGTCCAAGGGCAAGTACGTGGCGCTGAAGGATCTGGACGTGAAGTCCCGCCGCGAGCTGAA
CCGCCTGTGGTCGCAAGTGAAGATCGGCCGCTGA

Upstream 100 bases:

>100_bases
GCTCTGAGTCATCGCCGCCAGCGGCAAACGTTTTTCCCGACCCCTGCCCGGCAGGGGTTAGTTTTTGCAACGCCAAATAA
TCAATTAGGATTTTCCGTAT

Downstream 100 bases:

>100_bases
GCGGCCGCCTGATGAAAAACGCCGGCAACCCGCCGGCGTTTTTCATTCCAGGACCACCGTCACCCGGGGCCAGCGCGCCT
GGTAGCGCTTCTGCGCCACC

Product: polyamine ABC transporter system, substrate-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MNKIALAALLAVACGSAAAEDVLHIYNWNNALSPDTAKRFEQSCKCKLVQDYYGDNEEMLAKLAAGAKGYDMVFPTAFAV
NTLLKQGKLQPLDKSRLPNWKNLNSGYLALNQPFDPGNRYAAPTVVSLTLIGYNATQLKKAGVEGKSNSWALVFDPALLA
KIKGKVTVLDSQRELMAAALMYLGKDANSTNPADWKAAAETIRKAKPYWAAFNNQSYIKELTVGNIWVALGYSNDLFQAQ
QDAKNAKRPFELAYRPQQEGNVLAIDNMTILKDAPRPDLAHKFINFMLDGKNASEISNQIGATNPVKAAEAFFKPQIKAN
PVIMLDPSKGKYVALKDLDVKSRRELNRLWSQVKIGR

Sequences:

>Translated_357_residues
MNKIALAALLAVACGSAAAEDVLHIYNWNNALSPDTAKRFEQSCKCKLVQDYYGDNEEMLAKLAAGAKGYDMVFPTAFAV
NTLLKQGKLQPLDKSRLPNWKNLNSGYLALNQPFDPGNRYAAPTVVSLTLIGYNATQLKKAGVEGKSNSWALVFDPALLA
KIKGKVTVLDSQRELMAAALMYLGKDANSTNPADWKAAAETIRKAKPYWAAFNNQSYIKELTVGNIWVALGYSNDLFQAQ
QDAKNAKRPFELAYRPQQEGNVLAIDNMTILKDAPRPDLAHKFINFMLDGKNASEISNQIGATNPVKAAEAFFKPQIKAN
PVIMLDPSKGKYVALKDLDVKSRRELNRLWSQVKIGR
>Mature_357_residues
MNKIALAALLAVACGSAAAEDVLHIYNWNNALSPDTAKRFEQSCKCKLVQDYYGDNEEMLAKLAAGAKGYDMVFPTAFAV
NTLLKQGKLQPLDKSRLPNWKNLNSGYLALNQPFDPGNRYAAPTVVSLTLIGYNATQLKKAGVEGKSNSWALVFDPALLA
KIKGKVTVLDSQRELMAAALMYLGKDANSTNPADWKAAAETIRKAKPYWAAFNNQSYIKELTVGNIWVALGYSNDLFQAQ
QDAKNAKRPFELAYRPQQEGNVLAIDNMTILKDAPRPDLAHKFINFMLDGKNASEISNQIGATNPVKAAEAFFKPQIKAN
PVIMLDPSKGKYVALKDLDVKSRRELNRLWSQVKIGR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=363, Percent_Identity=36.6391184573003, Blast_Score=220, Evalue=9e-59,
Organism=Escherichia coli, GI1787367, Length=316, Percent_Identity=29.746835443038, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39374; Mature: 39374

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIALAALLAVACGSAAAEDVLHIYNWNNALSPDTAKRFEQSCKCKLVQDYYGDNEEML
CCHHHHHHHHHHHHCCHHHHHEEEEECCCCCCCCHHHHHHHHHHCEEEEHHHHCCCHHHH
AKLAAGAKGYDMVFPTAFAVNTLLKQGKLQPLDKSRLPNWKNLNSGYLALNQPFDPGNRY
HHHHCCCCCCEEHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCEEEECCCCCCCCCC
AAPTVVSLTLIGYNATQLKKAGVEGKSNSWALVFDPALLAKIKGKVTVLDSQRELMAAAL
CCCEEEEEEEECCCHHHHHHCCCCCCCCCEEEEECHHHHHHHCCCEEEECCHHHHHHHHH
MYLGKDANSTNPADWKAAAETIRKAKPYWAAFNNQSYIKELTVGNIWVALGYSNDLFQAQ
HHHCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCHHHHHEEECCEEEEECCCCHHHHHH
QDAKNAKRPFELAYRPQQEGNVLAIDNMTILKDAPRPDLAHKFINFMLDGKNASEISNQI
HHHHHCCCCEEEEECCCCCCCEEEECCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHC
GATNPVKAAEAFFKPQIKANPVIMLDPSKGKYVALKDLDVKSRRELNRLWSQVKIGR
CCCCCHHHHHHHHCCCCCCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKIALAALLAVACGSAAAEDVLHIYNWNNALSPDTAKRFEQSCKCKLVQDYYGDNEEML
CCHHHHHHHHHHHHCCHHHHHEEEEECCCCCCCCHHHHHHHHHHCEEEEHHHHCCCHHHH
AKLAAGAKGYDMVFPTAFAVNTLLKQGKLQPLDKSRLPNWKNLNSGYLALNQPFDPGNRY
HHHHCCCCCCEEHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCEEEECCCCCCCCCC
AAPTVVSLTLIGYNATQLKKAGVEGKSNSWALVFDPALLAKIKGKVTVLDSQRELMAAAL
CCCEEEEEEEECCCHHHHHHCCCCCCCCCEEEEECHHHHHHHCCCEEEECCHHHHHHHHH
MYLGKDANSTNPADWKAAAETIRKAKPYWAAFNNQSYIKELTVGNIWVALGYSNDLFQAQ
HHHCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCHHHHHEEECCEEEEECCCCHHHHHH
QDAKNAKRPFELAYRPQQEGNVLAIDNMTILKDAPRPDLAHKFINFMLDGKNASEISNQI
HHHHHCCCCEEEEECCCCCCCEEEECCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHC
GATNPVKAAEAFFKPQIKANPVIMLDPSKGKYVALKDLDVKSRRELNRLWSQVKIGR
CCCCCHHHHHHHHCCCCCCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]