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The map label for this gene is proB

Identifier: 34499667

GI number: 34499667

Start: 4546401

End: 4547519

Strand: Direct

Name: proB

Synonym: CV_4212

Alternate gene names: 34499667

Gene position: 4546401-4547519 (Clockwise)

Preceding gene: 34499666

Following gene: 34499668

Centisome position: 95.69

GC content: 69.26

Gene sequence:

>1119_bases
ATGCACTCGGTGATTCACGCTTCAGACCGCATCGTGGTCAAGGTAGGCTCCAGCCTGGTAACCAACGACGGCAGGGGGCT
GGACCTGAACGCGCTGGCGCGCTGGGCGGAGGAAGTTGCCGAACTGAAACGCCGCGGCAAGCAGGTGGTGCTGGTCTCCA
GCGGCGCCATCGCCGAAGGCTGCCAGCGCCTGGGCTGGACGGTGCGCCCGAAGGGCGTGCATGAGCTGCAGGCCGCAGCC
GCCGTCGGCCAGATGGGCCTGTGCCAGGCCTACGAGAGCGCGTTCCGCTCCTTCGGCCTGCGCACCGCGCAGATCCTGCT
GACCCACGAAGACCTGGCCGACCGCACCCGCTACCTGAACGCCCGCTCCACGCTGACCAGCCTGCTGAATCTGAACGTGG
TGCCCATCATCAACGAGAATGACACCGTGGCCACCAGCGAAATCCGCTTCGGCGACAACGACACGCTGGGCGCGCTGGTG
ACCAACCTGATCGAAGCCGACGCCCTGGTGATCCTGACCGACCAGCGCGGACTCTACAGCGCCGACCCGCGCAAGCATCC
CGACGCCGAGTTCATCCACCAGGCCGAAGCCGGCGACGAGCGCCTGGAGGACATGGCCGGCGGCGCCGGTTCCAGCGTCG
GCACCGGCGGCATGATCACCAAGATCCTGGCCGCCAAGCGCGCCGCCCGCAGCGGCGCCGCCACCGTGATCGCCAGCGGC
CGCGAACCCCATGTGCTGTCTCGGCTGGCCGACGGCGAAGCCATCGGCACCCAGCTGGTCGCCGCCACCAACCGCATGGC
CGCCCGCAAGCAGTGGCTGGCCGACCACCTGAAGCTGGCCGGCCGCCTGCTGCTGGACGACGGCGCCGCGCTGGCGATAC
GCGAGCGCGGCACCAGCCTGCTGCCGGTGGGCGTCAGCGCGGTGGAAGGCGATTTCCTGCGCGGCGAGGCGGTGGCCTGC
GTCGACGCGGCGGGGCACGAGGTGGCGCGCGGCCTGGTCAACTACAGCTCGGACGAAGCGCGTCAGATCATGCGCAAAAG
CACGCGCGAGATCGAAGCCGCGCTCGGTTACATCGTAGAACCGGAGTTGATCCACCGGGACAATATGGTCGCGCTCTGA

Upstream 100 bases:

>100_bases
ATACCGCCCCAGCGAGCAATTTTGACCTGGCGCAAGCGCCGGGCTTTTTCAGTTACACTGCCCTCTCTATAATTATTCGC
TTTCATAGAGAGGTCCGTTG

Downstream 100 bases:

>100_bases
GTCATCGCCGCCAGCGGCAAACGTTTTTCCCGACCCCTGCCCGGCAGGGGTTAGTTTTTGCAACGCCAAATAATCAATTA
GGATTTTCCGTATATGAACA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MHSVIHASDRIVVKVGSSLVTNDGRGLDLNALARWAEEVAELKRRGKQVVLVSSGAIAEGCQRLGWTVRPKGVHELQAAA
AVGQMGLCQAYESAFRSFGLRTAQILLTHEDLADRTRYLNARSTLTSLLNLNVVPIINENDTVATSEIRFGDNDTLGALV
TNLIEADALVILTDQRGLYSADPRKHPDAEFIHQAEAGDERLEDMAGGAGSSVGTGGMITKILAAKRAARSGAATVIASG
REPHVLSRLADGEAIGTQLVAATNRMAARKQWLADHLKLAGRLLLDDGAALAIRERGTSLLPVGVSAVEGDFLRGEAVAC
VDAAGHEVARGLVNYSSDEARQIMRKSTREIEAALGYIVEPELIHRDNMVAL

Sequences:

>Translated_372_residues
MHSVIHASDRIVVKVGSSLVTNDGRGLDLNALARWAEEVAELKRRGKQVVLVSSGAIAEGCQRLGWTVRPKGVHELQAAA
AVGQMGLCQAYESAFRSFGLRTAQILLTHEDLADRTRYLNARSTLTSLLNLNVVPIINENDTVATSEIRFGDNDTLGALV
TNLIEADALVILTDQRGLYSADPRKHPDAEFIHQAEAGDERLEDMAGGAGSSVGTGGMITKILAAKRAARSGAATVIASG
REPHVLSRLADGEAIGTQLVAATNRMAARKQWLADHLKLAGRLLLDDGAALAIRERGTSLLPVGVSAVEGDFLRGEAVAC
VDAAGHEVARGLVNYSSDEARQIMRKSTREIEAALGYIVEPELIHRDNMVAL
>Mature_372_residues
MHSVIHASDRIVVKVGSSLVTNDGRGLDLNALARWAEEVAELKRRGKQVVLVSSGAIAEGCQRLGWTVRPKGVHELQAAA
AVGQMGLCQAYESAFRSFGLRTAQILLTHEDLADRTRYLNARSTLTSLLNLNVVPIINENDTVATSEIRFGDNDTLGALV
TNLIEADALVILTDQRGLYSADPRKHPDAEFIHQAEAGDERLEDMAGGAGSSVGTGGMITKILAAKRAARSGAATVIASG
REPHVLSRLADGEAIGTQLVAATNRMAARKQWLADHLKLAGRLLLDDGAALAIRERGTSLLPVGVSAVEGDFLRGEAVAC
VDAAGHEVARGLVNYSSDEARQIMRKSTREIEAALGYIVEPELIHRDNMVAL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI62912457, Length=282, Percent_Identity=36.5248226950355, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI21361368, Length=284, Percent_Identity=36.2676056338028, Blast_Score=144, Evalue=1e-34,
Organism=Escherichia coli, GI1786437, Length=363, Percent_Identity=43.801652892562, Blast_Score=294, Evalue=5e-81,
Organism=Caenorhabditis elegans, GI17569967, Length=316, Percent_Identity=35.4430379746835, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17569969, Length=316, Percent_Identity=35.4430379746835, Blast_Score=158, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6320506, Length=415, Percent_Identity=35.6626506024096, Blast_Score=213, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6321822, Length=414, Percent_Identity=33.8164251207729, Blast_Score=196, Evalue=5e-51,
Organism=Drosophila melanogaster, GI21357643, Length=286, Percent_Identity=34.6153846153846, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_CHRVO (Q7NQC8)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903882.1
- ProteinModelPortal:   Q7NQC8
- GeneID:   2549617
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4212
- NMPDR:   fig|243365.1.peg.4212
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   Q7NQC8
- ProtClustDB:   PRK05429
- BioCyc:   CVIO243365:CV_4212-MONOMER
- BRENDA:   2.7.2.11
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39675; Mature: 39675

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSVIHASDRIVVKVGSSLVTNDGRGLDLNALARWAEEVAELKRRGKQVVLVSSGAIAEG
CCCCEECCCEEEEEECCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHH
CQRLGWTVRPKGVHELQAAAAVGQMGLCQAYESAFRSFGLRTAQILLTHEDLADRTRYLN
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHH
ARSTLTSLLNLNVVPIINENDTVATSEIRFGDNDTLGALVTNLIEADALVILTDQRGLYS
HHHHHHHHHCCCEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCC
ADPRKHPDAEFIHQAEAGDERLEDMAGGAGSSVGTGGMITKILAAKRAARSGAATVIASG
CCCCCCCCHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECC
REPHVLSRLADGEAIGTQLVAATNRMAARKQWLADHLKLAGRLLLDDGAALAIRERGTSL
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEEECCCCEE
LPVGVSAVEGDFLRGEAVACVDAAGHEVARGLVNYSSDEARQIMRKSTREIEAALGYIVE
EECCHHHHCCCCCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
PELIHRDNMVAL
CHHHCCCCCCCC
>Mature Secondary Structure
MHSVIHASDRIVVKVGSSLVTNDGRGLDLNALARWAEEVAELKRRGKQVVLVSSGAIAEG
CCCCEECCCEEEEEECCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHH
CQRLGWTVRPKGVHELQAAAAVGQMGLCQAYESAFRSFGLRTAQILLTHEDLADRTRYLN
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHH
ARSTLTSLLNLNVVPIINENDTVATSEIRFGDNDTLGALVTNLIEADALVILTDQRGLYS
HHHHHHHHHCCCEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCC
ADPRKHPDAEFIHQAEAGDERLEDMAGGAGSSVGTGGMITKILAAKRAARSGAATVIASG
CCCCCCCCHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECC
REPHVLSRLADGEAIGTQLVAATNRMAARKQWLADHLKLAGRLLLDDGAALAIRERGTSL
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEEECCCCEE
LPVGVSAVEGDFLRGEAVACVDAAGHEVARGLVNYSSDEARQIMRKSTREIEAALGYIVE
EECCHHHHCCCCCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
PELIHRDNMVAL
CHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782