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The map label for this gene is rpoA

Identifier: 34499615

GI number: 34499615

Start: 4502011

End: 4502994

Strand: Reverse

Name: rpoA

Synonym: CV_4160

Alternate gene names: 34499615

Gene position: 4502994-4502011 (Counterclockwise)

Preceding gene: 34499616

Following gene: 34499614

Centisome position: 94.78

GC content: 58.74

Gene sequence:

>984_bases
ATGCAAAACAGCGCTTCGGAATTTCTGAAACCTCGTCTGATCGACGTACAGCCGGTTTCCGCCACTCACGCTCGCGTGTC
GATGGAGCCGTTCGAGCGCGGCTATGCCCATACCCTGGGCAATTCGCTGCGCCGTATCCTGCTGTCGTCGATGCCGGGCT
ATGCTCCGACCGAAGTGACTATCGCTGGCGTTTTGCATGAGTATTCCGCGCTTGATGGCGTGCGCGAGGATGTCGTCGAC
ATCCTCCTCAACCTCAAGGGCGTAGTGCTTAAGCTGCATGGTCGCGACAGTGTCGTGCTGACCTTGAAAAAGGAAGGCGA
GGGCGCTGTCTTGGCTAGCGATATCGAGCTGCCGCATGATGTGGAAGTGATCAATCCGGAACACGTGATTTGTCACATCT
CCTCCGGCGGCAAGATCGACATGGAGGTCAAGGTGGAGAAGGGCCGCGGCTATCAGCCGGTGTCCGCTCGCACCAGCCAT
GACGACAACCGTTCCATCGGTACCATCCAACTGGACGCTTCCTTCTCGCCGGTCCGCCGCGTGAGCTTCGCTGTGGAAAG
CGCCCGCGTGGAACAGCGTACCGACCTCGACCGCCTGGTTCTCGATATCGAGACCAACGGCGTGATCGAGCCGGAGCAGG
CTGTGCGCAACGCAGCCCGCATCCTGATGGATCAGCTGTCGATCTTTGCCGACCTCCAGGGCACAGCGGTTGAAGAAGTC
GTTGAAAAGGCGCCGCCGATCGATCCGATCCTGCTGCGTCCGGTAGACGATCTTGAACTGACGGTCCGTTCGGCCAACTG
CCTGAAAGCAGAGAACATTTATTACATCGGCGATCTGATCCAGCGCACCGAAACCGAGCTTCTGAAGACTCCGAACCTCG
GCCGCAAGTCGCTGAACGAGATCAAGGAAGTTCTCGCTTCCAAGGGCCTGACTCTCGGCATGAAGCTCGAGAATTGGCCT
CCGGCTGGTCTGGAAAAGCCGTAA

Upstream 100 bases:

>100_bases
TCAAATCCGCTCCGGAACGTTCCGAACTGTCTAGCGATATCAACGAACAACTCGTTGTGGAATTCTACTCCAAGTAATCG
CTAGCTCTTAGGGAATGACT

Downstream 100 bases:

>100_bases
GTTTGAGACTAAAGGACATTAACAATGCGTCATCGTTACAGTAATCGTAAACTGAACCGCACGACCAGCCATCGTCTGGC
GATGCTGCGCAACATGGCCA

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MQNSASEFLKPRLIDVQPVSATHARVSMEPFERGYAHTLGNSLRRILLSSMPGYAPTEVTIAGVLHEYSALDGVREDVVD
ILLNLKGVVLKLHGRDSVVLTLKKEGEGAVLASDIELPHDVEVINPEHVICHISSGGKIDMEVKVEKGRGYQPVSARTSH
DDNRSIGTIQLDASFSPVRRVSFAVESARVEQRTDLDRLVLDIETNGVIEPEQAVRNAARILMDQLSIFADLQGTAVEEV
VEKAPPIDPILLRPVDDLELTVRSANCLKAENIYYIGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGMKLENWP
PAGLEKP

Sequences:

>Translated_327_residues
MQNSASEFLKPRLIDVQPVSATHARVSMEPFERGYAHTLGNSLRRILLSSMPGYAPTEVTIAGVLHEYSALDGVREDVVD
ILLNLKGVVLKLHGRDSVVLTLKKEGEGAVLASDIELPHDVEVINPEHVICHISSGGKIDMEVKVEKGRGYQPVSARTSH
DDNRSIGTIQLDASFSPVRRVSFAVESARVEQRTDLDRLVLDIETNGVIEPEQAVRNAARILMDQLSIFADLQGTAVEEV
VEKAPPIDPILLRPVDDLELTVRSANCLKAENIYYIGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGMKLENWP
PAGLEKP
>Mature_327_residues
MQNSASEFLKPRLIDVQPVSATHARVSMEPFERGYAHTLGNSLRRILLSSMPGYAPTEVTIAGVLHEYSALDGVREDVVD
ILLNLKGVVLKLHGRDSVVLTLKKEGEGAVLASDIELPHDVEVINPEHVICHISSGGKIDMEVKVEKGRGYQPVSARTSH
DDNRSIGTIQLDASFSPVRRVSFAVESARVEQRTDLDRLVLDIETNGVIEPEQAVRNAARILMDQLSIFADLQGTAVEEV
VEKAPPIDPILLRPVDDLELTVRSANCLKAENIYYIGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGMKLENWP
PAGLEKP

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=326, Percent_Identity=63.1901840490798, Blast_Score=430, Evalue=1e-122,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_CHRVO (Q7NQH7)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903830.1
- ProteinModelPortal:   Q7NQH7
- GeneID:   2549797
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4160
- NMPDR:   fig|243365.1.peg.4160
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- PhylomeDB:   Q7NQH7
- ProtClustDB:   PRK05182
- BioCyc:   CVIO243365:CV_4160-MONOMER
- BRENDA:   2.7.7.6
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 35972; Mature: 35972

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNSASEFLKPRLIDVQPVSATHARVSMEPFERGYAHTLGNSLRRILLSSMPGYAPTEVT
CCCCHHHHCCCCEEEECCCCCCHHEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
IAGVLHEYSALDGVREDVVDILLNLKGVVLKLHGRDSVVLTLKKEGEGAVLASDIELPHD
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEEEECCCCCEEEECCCCCCC
VEVINPEHVICHISSGGKIDMEVKVEKGRGYQPVSARTSHDDNRSIGTIQLDASFSPVRR
EEEECCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHH
VSFAVESARVEQRTDLDRLVLDIETNGVIEPEQAVRNAARILMDQLSIFADLQGTAVEEV
HHHHHHHHHHHHHCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VEKAPPIDPILLRPVDDLELTVRSANCLKAENIYYIGDLIQRTETELLKTPNLGRKSLNE
HHHCCCCCCEEECCCCCCEEEEECCCCEEECCEEEHHHHHHHHHHHHHCCCCCCHHHHHH
IKEVLASKGLTLGMKLENWPPAGLEKP
HHHHHHHCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MQNSASEFLKPRLIDVQPVSATHARVSMEPFERGYAHTLGNSLRRILLSSMPGYAPTEVT
CCCCHHHHCCCCEEEECCCCCCHHEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
IAGVLHEYSALDGVREDVVDILLNLKGVVLKLHGRDSVVLTLKKEGEGAVLASDIELPHD
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEEEECCCCCEEEECCCCCCC
VEVINPEHVICHISSGGKIDMEVKVEKGRGYQPVSARTSHDDNRSIGTIQLDASFSPVRR
EEEECCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHH
VSFAVESARVEQRTDLDRLVLDIETNGVIEPEQAVRNAARILMDQLSIFADLQGTAVEEV
HHHHHHHHHHHHHCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VEKAPPIDPILLRPVDDLELTVRSANCLKAENIYYIGDLIQRTETELLKTPNLGRKSLNE
HHHCCCCCCEEECCCCCCEEEEECCCCEEECCEEEHHHHHHHHHHHHHCCCCCCHHHHHH
IKEVLASKGLTLGMKLENWPPAGLEKP
HHHHHHHCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782