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The map label for this gene is rluB [H]

Identifier: 34499601

GI number: 34499601

Start: 4486795

End: 4487496

Strand: Direct

Name: rluB [H]

Synonym: CV_4146

Alternate gene names: 34499601

Gene position: 4486795-4487496 (Clockwise)

Preceding gene: 34499600

Following gene: 34499602

Centisome position: 94.44

GC content: 69.66

Gene sequence:

>702_bases
ATGGACGAGACGAGCGTGCGCCTTTCCAAGCGCATGGTGGAGCTGGGGCTGTGCTCGCGGCGCGAGGCGGATGCCTGCAT
CGAGCAAGGCCTGGTGAAGGTGGACGGCAAAGTGGTGAATACCCTGGGCGCGCGCGTTCGGCCGGAGCAGCAAATCACCC
TGGCCGGCAAACCGCAGGCCATGGCGACGCTGCCGGTGACGATGCTGCTGAACCGCCCTGCCGGCGAGGAGGCTCCGCCG
GCGCAGCTGCTGGGGCCGGACAGCCGCGCGCCGCAGGACGGCGCGGAACAGGTCTGGCTGGCGCGCCACCGCCAGCACCT
GACCGCCGTCGGGGCCGTGGATGAGCGTTGCCACGGCCTGCTGGTCCTGACCCAGGATAAAAAACTGCCGCGCCATCTGG
CCGAGTGCGAGATGGAATTCCTGCTGCAAGTGGAGCAGGCCCCGGCGGCGGACGCGCTGAAAGCTCGCGTCGCCGCCATC
CGCCTGGACGGCAAGCCCTTGCGCCAGTGCAAGATCAGCCGCCAGAGCGACCATCAGCTGCGCTGCGCGGTTTACGCGCC
GCGCGCCGGCTTCTTCGCCGAAATCAGCCGCCAGCTCGGCGTCAGCCTGCAAAGCGCGCGCTGCATCCGCATCGGCCGCC
TGGCCTTGGCCGGCCTGCAGCCGGGACAATGGCGCTACCTGCAGCCGTTCGAACGTTTCTGA

Upstream 100 bases:

>100_bases
GCGCGCCCTGCTGCCCTCCCGTTCCGCCTGAGCCCCGGCCCGTTGCCAGCCCCCCGGCCGGCAACGGATAATCGCCGTCC
GTTTGATGAGGATATCGACA

Downstream 100 bases:

>100_bases
TCCGCCCTATTCGACGGCGACGCGCATTGCCCTGCCGCCGCGGGCCTTCATGCCAAGCCGAACCGCTCGCGGGGTGGGTT
CGCGCCAAAATGAAGGTTGA

Product: pseudouridylate synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MDETSVRLSKRMVELGLCSRREADACIEQGLVKVDGKVVNTLGARVRPEQQITLAGKPQAMATLPVTMLLNRPAGEEAPP
AQLLGPDSRAPQDGAEQVWLARHRQHLTAVGAVDERCHGLLVLTQDKKLPRHLAECEMEFLLQVEQAPAADALKARVAAI
RLDGKPLRQCKISRQSDHQLRCAVYAPRAGFFAEISRQLGVSLQSARCIRIGRLALAGLQPGQWRYLQPFERF

Sequences:

>Translated_233_residues
MDETSVRLSKRMVELGLCSRREADACIEQGLVKVDGKVVNTLGARVRPEQQITLAGKPQAMATLPVTMLLNRPAGEEAPP
AQLLGPDSRAPQDGAEQVWLARHRQHLTAVGAVDERCHGLLVLTQDKKLPRHLAECEMEFLLQVEQAPAADALKARVAAI
RLDGKPLRQCKISRQSDHQLRCAVYAPRAGFFAEISRQLGVSLQSARCIRIGRLALAGLQPGQWRYLQPFERF
>Mature_233_residues
MDETSVRLSKRMVELGLCSRREADACIEQGLVKVDGKVVNTLGARVRPEQQITLAGKPQAMATLPVTMLLNRPAGEEAPP
AQLLGPDSRAPQDGAEQVWLARHRQHLTAVGAVDERCHGLLVLTQDKKLPRHLAECEMEFLLQVEQAPAADALKARVAAI
RLDGKPLRQCKISRQSDHQLRCAVYAPRAGFFAEISRQLGVSLQSARCIRIGRLALAGLQPGQWRYLQPFERF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=239, Percent_Identity=27.6150627615063, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 25767; Mature: 25767

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDETSVRLSKRMVELGLCSRREADACIEQGLVKVDGKVVNTLGARVRPEQQITLAGKPQA
CCCHHHHHHHHHHHHHCCCCCHHHHHHHCCEEHCCCCHHHHHCCCCCCCCEEEECCCCCC
MATLPVTMLLNRPAGEEAPPAQLLGPDSRAPQDGAEQVWLARHRQHLTAVGAVDERCHGL
HHHHHHHHEEECCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHCCCEE
LVLTQDKKLPRHLAECEMEFLLQVEQAPAADALKARVAAIRLDGKPLRQCKISRQSDHQL
EEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEECCCHHHHHCCCCCCCCEE
RCAVYAPRAGFFAEISRQLGVSLQSARCIRIGRLALAGLQPGQWRYLQPFERF
EEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHCC
>Mature Secondary Structure
MDETSVRLSKRMVELGLCSRREADACIEQGLVKVDGKVVNTLGARVRPEQQITLAGKPQA
CCCHHHHHHHHHHHHHCCCCCHHHHHHHCCEEHCCCCHHHHHCCCCCCCCEEEECCCCCC
MATLPVTMLLNRPAGEEAPPAQLLGPDSRAPQDGAEQVWLARHRQHLTAVGAVDERCHGL
HHHHHHHHEEECCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHCCCEE
LVLTQDKKLPRHLAECEMEFLLQVEQAPAADALKARVAAIRLDGKPLRQCKISRQSDHQL
EEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEECCCHHHHHCCCCCCCCEE
RCAVYAPRAGFFAEISRQLGVSLQSARCIRIGRLALAGLQPGQWRYLQPFERF
EEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]