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The map label for this gene is potB [H]

Identifier: 34499556

GI number: 34499556

Start: 4442577

End: 4443464

Strand: Reverse

Name: potB [H]

Synonym: CV_4101

Alternate gene names: 34499556

Gene position: 4443464-4442577 (Counterclockwise)

Preceding gene: 34499557

Following gene: 34499555

Centisome position: 93.53

GC content: 64.64

Gene sequence:

>888_bases
ATGCGTGATCGTTTCGGCAAGTGGGCGCTGTCCTGGCCCCCCTTGCTGTATCTGGTATTGTTCTTCCTGATTCCGTCGCT
GATCATGCTGGTGGCCGCGTTCCGCCAGCCCGGCGACTACGGCGGCCTGGCGCCGCTGTTCACCATCGAGGACGGCCAGC
GCGTCTGGCAGCTGACGCTGGACAATTTCCACCGCCTGGTGGAGGAGCCGCTGTACATCGAGCTGTTCATCAAGTCGGCC
GGCTACGCGCTGACCACCACCGTGGTCTGCCTGCTGATGGCTTATCCGCTGGCCTGGCTGATCGCCCGCTCCGGCAAGAA
GTACCGCGACCTGCTGCTGCTGTTGGTGATCCTGCCGTTCTGGTCCAACTTCCTGATCCGCATCTACGCGTGGATGATCA
TCCTGGGGCCGCAGTCGGCGTTCACCAAGGCGCTGAACCTGGTGCTGACCACGCTGGGCTTCGAGCCGGTGCGGCTGCTG
TTCACCTCGTTCGCGGTGATCGTCGGCCTGGTCTACGTGCATCTGCCCTTCATGGTGCTGCCGCTGTACGCCAACCTGGA
AAAGCACGACATGGCCCTGCTCGACGCCGCGCAGGATCTGGGCGCCAACGCCTGGCAGCGCTTCTGGCGCGTGACCTGGC
CGCTGAGCCTGCCCGGCGTGTTCGCCGGTTCGGCGCTGGTGTTCATCCCGGCCTTGGGCATGTTCGCCATTCCGGACATT
CTCGGCGGCACCGAGTCCATCATGATAGGCAACCTGATCAAGCAGCAGATCCTGGACACCCGCGATTGGCCGTTCGGCTC
GGTGCTGTCGATCATGCTGACCGGCGGCGTGCTGCTGATCGCCGTGCTGGGGGCCGTGGTGGCCAGAAAGGGGAAGGCGC
GTGGCTAA

Upstream 100 bases:

>100_bases
CAACATTCCCGGCGTGGCCAAGTTCTTCGACGACGGCGACGTGGTCGAGATCGCCTGGCGCTTCGACGCCGGCAGCTTCC
TGACGGAGTAAGCGATGGCG

Downstream 100 bases:

>100_bases
GAAACAATCGAAATGGCTGTGGGCTTCGGCCGGCCTGACCTATCTGTTCCTCTACCTGCCGCTGGTCATCGTGGTGCTGT
ACTCGTTCAACGATTCGCGG

Product: polyamine transport protein PotB

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MRDRFGKWALSWPPLLYLVLFFLIPSLIMLVAAFRQPGDYGGLAPLFTIEDGQRVWQLTLDNFHRLVEEPLYIELFIKSA
GYALTTTVVCLLMAYPLAWLIARSGKKYRDLLLLLVILPFWSNFLIRIYAWMIILGPQSAFTKALNLVLTTLGFEPVRLL
FTSFAVIVGLVYVHLPFMVLPLYANLEKHDMALLDAAQDLGANAWQRFWRVTWPLSLPGVFAGSALVFIPALGMFAIPDI
LGGTESIMIGNLIKQQILDTRDWPFGSVLSIMLTGGVLLIAVLGAVVARKGKARG

Sequences:

>Translated_295_residues
MRDRFGKWALSWPPLLYLVLFFLIPSLIMLVAAFRQPGDYGGLAPLFTIEDGQRVWQLTLDNFHRLVEEPLYIELFIKSA
GYALTTTVVCLLMAYPLAWLIARSGKKYRDLLLLLVILPFWSNFLIRIYAWMIILGPQSAFTKALNLVLTTLGFEPVRLL
FTSFAVIVGLVYVHLPFMVLPLYANLEKHDMALLDAAQDLGANAWQRFWRVTWPLSLPGVFAGSALVFIPALGMFAIPDI
LGGTESIMIGNLIKQQILDTRDWPFGSVLSIMLTGGVLLIAVLGAVVARKGKARG
>Mature_295_residues
MRDRFGKWALSWPPLLYLVLFFLIPSLIMLVAAFRQPGDYGGLAPLFTIEDGQRVWQLTLDNFHRLVEEPLYIELFIKSA
GYALTTTVVCLLMAYPLAWLIARSGKKYRDLLLLLVILPFWSNFLIRIYAWMIILGPQSAFTKALNLVLTTLGFEPVRLL
FTSFAVIVGLVYVHLPFMVLPLYANLEKHDMALLDAAQDLGANAWQRFWRVTWPLSLPGVFAGSALVFIPALGMFAIPDI
LGGTESIMIGNLIKQQILDTRDWPFGSVLSIMLTGGVLLIAVLGAVVARKGKARG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=260, Percent_Identity=45.3846153846154, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1787080, Length=225, Percent_Identity=47.1111111111111, Blast_Score=197, Evalue=6e-52,
Organism=Escherichia coli, GI1787713, Length=249, Percent_Identity=30.1204819277108, Blast_Score=108, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32970; Mature: 32970

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDRFGKWALSWPPLLYLVLFFLIPSLIMLVAAFRQPGDYGGLAPLFTIEDGQRVWQLTL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHH
DNFHRLVEEPLYIELFIKSAGYALTTTVVCLLMAYPLAWLIARSGKKYRDLLLLLVILPF
HHHHHHHCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
WSNFLIRIYAWMIILGPQSAFTKALNLVLTTLGFEPVRLLFTSFAVIVGLVYVHLPFMVL
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
PLYANLEKHDMALLDAAQDLGANAWQRFWRVTWPLSLPGVFAGSALVFIPALGMFAIPDI
HHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHCHHHHHHHHHHHHHHHHH
LGGTESIMIGNLIKQQILDTRDWPFGSVLSIMLTGGVLLIAVLGAVVARKGKARG
HCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRDRFGKWALSWPPLLYLVLFFLIPSLIMLVAAFRQPGDYGGLAPLFTIEDGQRVWQLTL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHH
DNFHRLVEEPLYIELFIKSAGYALTTTVVCLLMAYPLAWLIARSGKKYRDLLLLLVILPF
HHHHHHHCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
WSNFLIRIYAWMIILGPQSAFTKALNLVLTTLGFEPVRLLFTSFAVIVGLVYVHLPFMVL
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
PLYANLEKHDMALLDAAQDLGANAWQRFWRVTWPLSLPGVFAGSALVFIPALGMFAIPDI
HHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHCHHHHHHHHHHHHHHHHH
LGGTESIMIGNLIKQQILDTRDWPFGSVLSIMLTGGVLLIAVLGAVVARKGKARG
HCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]