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The map label for this gene is ycaN [H]

Identifier: 34499543

GI number: 34499543

Start: 4424571

End: 4425533

Strand: Reverse

Name: ycaN [H]

Synonym: CV_4088

Alternate gene names: 34499543

Gene position: 4425533-4424571 (Counterclockwise)

Preceding gene: 34499546

Following gene: 34499532

Centisome position: 93.15

GC content: 63.86

Gene sequence:

>963_bases
TTGAAAGCACTGATGGTCTTCATGACCACTGCCGAGAACGGCAGTTTTTCCGACGCCGCGCGCAAGCTTGGCGTTTCGCC
TGCCGCCATCAGCCAGAGCATCGCCAGGCTGGAGCAGGAACTGGAAGTGCGACTGTTCAACCGCACCACGCGCCAGTTGA
CCCTGACCGAGGACGGCAGGCGCTTCTACGCGCAGTGCCGGGGTCCGGTCAACAACCTGGACTCCGCGATCAATCAGCTG
AAGGCCAGCCGCGACGAGCCGGCAGGCCATCTGCGCGTCAGCATGCCCAACTCTTTCGGCCGCCGCTTCATCTTGCCCAT
CATGGGCGAGTTCTGCGAGCGCTATCCGAAGGTGAGGGTGTTCTTCGGTCTGGACGACCATTTCAGCGATCTGATCGAGG
ACGGCTACGATGTCGGCGTGCGCACCGGCATGATGCCGGACAGCCGCATGGTGGCGCGCAATCTGGCGCAGATGCCGCTG
TACGTGGTGGCGTCGCCCGAATACTGGGATCAGCATGGCCGTCCGTCCTCTCCCGAGGAACTGAGCGGTTACGACTGCAT
CAACTTCCAGTTTCCCACTTCCGGCCGCCTGTTCAAGTGGGAGTTCGACCGCAAGGGCGAACGCATTCCGCTGGAGGTCA
ACGGCACTTACACGCTGAACGACATGGAGGCGGTGGCCGAGCTGGCGCGCCTCGGCATGGGTGTCGCGCAGCTGCCGGGC
CACGAGGTGGTGCACCTGATCCGCAGCGGCGAGCTGGAGCCGGTGTTGACCGACATGGTGTCGCTGGAGCGTTCGATCTA
CATCTGCTTCCCGCACCGCGACCACATCGCGCCGCGCACCCGCGTCTTCGTCGATTTCGTGGTGGAGAAGCTGGCCGACC
ATCCGGACATCATGGCGGATCTGCCTGGACTGGACCTGTCTGCCAAGCGCAGCGCGCTGCAACAGAGCGTGGGCGTGCTC
TGA

Upstream 100 bases:

>100_bases
AAACAGCTCCAATTCTGTGAACGATCCCACGGTTTGCCAGACCAAGCAGTTAGGCAAACATGCATGGATCGCTTCAGGAA
TCGCCCCGCTTATGGATACC

Downstream 100 bases:

>100_bases
CCGGCATCTCCCTCCGATAAGAACCGGCGCCTTGGCGCCGGTTTTTTCATGGCCGGACGGCGCAGCCTTCTCCGACCACC
CGGTCCCGCCCCGCCTGCTT

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MKALMVFMTTAENGSFSDAARKLGVSPAAISQSIARLEQELEVRLFNRTTRQLTLTEDGRRFYAQCRGPVNNLDSAINQL
KASRDEPAGHLRVSMPNSFGRRFILPIMGEFCERYPKVRVFFGLDDHFSDLIEDGYDVGVRTGMMPDSRMVARNLAQMPL
YVVASPEYWDQHGRPSSPEELSGYDCINFQFPTSGRLFKWEFDRKGERIPLEVNGTYTLNDMEAVAELARLGMGVAQLPG
HEVVHLIRSGELEPVLTDMVSLERSIYICFPHRDHIAPRTRVFVDFVVEKLADHPDIMADLPGLDLSAKRSALQQSVGVL

Sequences:

>Translated_320_residues
MKALMVFMTTAENGSFSDAARKLGVSPAAISQSIARLEQELEVRLFNRTTRQLTLTEDGRRFYAQCRGPVNNLDSAINQL
KASRDEPAGHLRVSMPNSFGRRFILPIMGEFCERYPKVRVFFGLDDHFSDLIEDGYDVGVRTGMMPDSRMVARNLAQMPL
YVVASPEYWDQHGRPSSPEELSGYDCINFQFPTSGRLFKWEFDRKGERIPLEVNGTYTLNDMEAVAELARLGMGVAQLPG
HEVVHLIRSGELEPVLTDMVSLERSIYICFPHRDHIAPRTRVFVDFVVEKLADHPDIMADLPGLDLSAKRSALQQSVGVL
>Mature_320_residues
MKALMVFMTTAENGSFSDAARKLGVSPAAISQSIARLEQELEVRLFNRTTRQLTLTEDGRRFYAQCRGPVNNLDSAINQL
KASRDEPAGHLRVSMPNSFGRRFILPIMGEFCERYPKVRVFFGLDDHFSDLIEDGYDVGVRTGMMPDSRMVARNLAQMPL
YVVASPEYWDQHGRPSSPEELSGYDCINFQFPTSGRLFKWEFDRKGERIPLEVNGTYTLNDMEAVAELARLGMGVAQLPG
HEVVHLIRSGELEPVLTDMVSLERSIYICFPHRDHIAPRTRVFVDFVVEKLADHPDIMADLPGLDLSAKRSALQQSVGVL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787128, Length=288, Percent_Identity=32.2916666666667, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=31.740614334471, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1787589, Length=283, Percent_Identity=29.6819787985866, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1789639, Length=306, Percent_Identity=29.7385620915033, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI87081978, Length=294, Percent_Identity=30.6122448979592, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=25.6756756756757, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789440, Length=289, Percent_Identity=29.0657439446367, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1788706, Length=299, Percent_Identity=24.7491638795987, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI145693105, Length=320, Percent_Identity=22.5, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1786448, Length=294, Percent_Identity=25.8503401360544, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082132, Length=259, Percent_Identity=25.4826254826255, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789173, Length=188, Percent_Identity=29.7872340425532, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI157672245, Length=139, Percent_Identity=33.8129496402878, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790011, Length=291, Percent_Identity=22.3367697594502, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788748, Length=119, Percent_Identity=31.0924369747899, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787530, Length=234, Percent_Identity=25.2136752136752, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787601, Length=115, Percent_Identity=30.4347826086957, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786508, Length=70, Percent_Identity=45.7142857142857, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1786713, Length=146, Percent_Identity=29.4520547945205, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790783, Length=259, Percent_Identity=26.6409266409266, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787879, Length=153, Percent_Identity=30.0653594771242, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36055; Mature: 36055

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALMVFMTTAENGSFSDAARKLGVSPAAISQSIARLEQELEVRLFNRTTRQLTLTEDGR
CCEEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCH
RFYAQCRGPVNNLDSAINQLKASRDEPAGHLRVSMPNSFGRRFILPIMGEFCERYPKVRV
HHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEHHHHHHHHHHHCCCEEE
FFGLDDHFSDLIEDGYDVGVRTGMMPDSRMVARNLAQMPLYVVASPEYWDQHGRPSSPEE
EECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCHHHHHCCCCCCCHH
LSGYDCINFQFPTSGRLFKWEFDRKGERIPLEVNGTYTLNDMEAVAELARLGMGVAQLPG
CCCCEEEEEECCCCCCEEEEEECCCCCEEEEEECCEEEECHHHHHHHHHHHCCCHHHCCC
HEVVHLIRSGELEPVLTDMVSLERSIYICFPHRDHIAPRTRVFVDFVVEKLADHPDIMAD
HHHHHHHHCCCCCHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHC
LPGLDLSAKRSALQQSVGVL
CCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKALMVFMTTAENGSFSDAARKLGVSPAAISQSIARLEQELEVRLFNRTTRQLTLTEDGR
CCEEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCH
RFYAQCRGPVNNLDSAINQLKASRDEPAGHLRVSMPNSFGRRFILPIMGEFCERYPKVRV
HHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEHHHHHHHHHHHCCCEEE
FFGLDDHFSDLIEDGYDVGVRTGMMPDSRMVARNLAQMPLYVVASPEYWDQHGRPSSPEE
EECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCHHHHHCCCCCCCHH
LSGYDCINFQFPTSGRLFKWEFDRKGERIPLEVNGTYTLNDMEAVAELARLGMGVAQLPG
CCCCEEEEEECCCCCCEEEEEECCCCCEEEEEECCEEEECHHHHHHHHHHHCCCHHHCCC
HEVVHLIRSGELEPVLTDMVSLERSIYICFPHRDHIAPRTRVFVDFVVEKLADHPDIMAD
HHHHHHHHCCCCCHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHC
LPGLDLSAKRSALQQSVGVL
CCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]