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The map label for this gene is iaaA [H]

Identifier: 34499502

GI number: 34499502

Start: 4377987

End: 4378922

Strand: Direct

Name: iaaA [H]

Synonym: CV_4047

Alternate gene names: 34499502

Gene position: 4377987-4378922 (Clockwise)

Preceding gene: 34499499

Following gene: 34499505

Centisome position: 92.15

GC content: 72.22

Gene sequence:

>936_bases
ATGACCATCGCCCTCGCCTTGCACGGCGGCGCCGGCACCCTGCGCCGCAGCGACATGGACGCCGAATTGGAGCGCGCCTA
CCGCGCCGGCCTGGAACAGGCGCTGGAGGCCGGCCACGCGGTGCTGCGCGGCGGCGGCGCGGCGCTGGATGCGGTGTGCG
CGGCGGTCTGCGCGCTGGAGGACGATCCACTGTTCAACGCCGGCCGCGGCTCGGTGTTCAATCTGGACGGCAAACAGGAA
ATGGAAGCGGCGGTGATGGACGGCATCCGCCAGGACGCCGGCAGCGTAGCCGGCCTTTCCGGCGTGCGCAACCCGGTGCT
GCTGGCGCGCGCGGTGATGGAGCGCACACCCCACGTGACGCTGGGGTATGCGGCGGCGGAAGATTTCGCCCGCCGGGCAG
GATTGGAAATGCGGCCGCCGGAATACTTCCACACCGACAAGCGCTGGCAGGCGCTGCTGGCGGAAAAGGAGAGGCTGGCC
AACGGCGGCTCGGACGAGGACATCTCCGAAGACCGCAAGCACGGCACCGTCGGCGCGGTGGCGCTGGACGCGCAAGGCCG
GCTGGCGGCCGCCACCTCCACCGGCGGCCGCACCGCCAAGTGGCCAGGCCGCATCGGCGACACGCCGGTGATCGGCGCCG
GCACCTGGGCCGACGCCTATTGCGCGGTGTCGGCCACCGGCCACGGCGAATACTTCGTCCGCGCCGCCGCCGGGCACGAG
ATCTCGGCCCGGATCCGCCACCTGGACGAAACCGTGGCCCAGGCTTGCGACGCCGTGGTCTACGGCCAGCTGCTGACCAT
GGGCGGCACCGGCGGCGTCGTCGCCGTCGACCGCTACGGCCGGGTGGCGTTGCCGTTCAACTGCGAGGGCATGTACCGCG
CGGCGATAGACGGCGCGGGCTTACGCACCGTCGCCATCTACCGCGGCGAAGACTAG

Upstream 100 bases:

>100_bases
GGCAAGCCGGCCACGCCCATCGTCTTCGTTGCGCATCGCTGGCGAACTCCCATCAAAAGCCTCATGCTAACAGTCCATTC
GCCACCCTGGAGATCGACAC

Downstream 100 bases:

>100_bases
CGGTAGGCGGCCTGCCACTCCGCCTGCAGCTGGGCGATCAGTTCCGCCGCCGGCATCTCGCGCGCGTCGGCGACGCCCTC
GCCCGCCCACAACGACATCA

Product: L-asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MTIALALHGGAGTLRRSDMDAELERAYRAGLEQALEAGHAVLRGGGAALDAVCAAVCALEDDPLFNAGRGSVFNLDGKQE
MEAAVMDGIRQDAGSVAGLSGVRNPVLLARAVMERTPHVTLGYAAAEDFARRAGLEMRPPEYFHTDKRWQALLAEKERLA
NGGSDEDISEDRKHGTVGAVALDAQGRLAAATSTGGRTAKWPGRIGDTPVIGAGTWADAYCAVSATGHGEYFVRAAAGHE
ISARIRHLDETVAQACDAVVYGQLLTMGGTGGVVAVDRYGRVALPFNCEGMYRAAIDGAGLRTVAIYRGED

Sequences:

>Translated_311_residues
MTIALALHGGAGTLRRSDMDAELERAYRAGLEQALEAGHAVLRGGGAALDAVCAAVCALEDDPLFNAGRGSVFNLDGKQE
MEAAVMDGIRQDAGSVAGLSGVRNPVLLARAVMERTPHVTLGYAAAEDFARRAGLEMRPPEYFHTDKRWQALLAEKERLA
NGGSDEDISEDRKHGTVGAVALDAQGRLAAATSTGGRTAKWPGRIGDTPVIGAGTWADAYCAVSATGHGEYFVRAAAGHE
ISARIRHLDETVAQACDAVVYGQLLTMGGTGGVVAVDRYGRVALPFNCEGMYRAAIDGAGLRTVAIYRGED
>Mature_310_residues
TIALALHGGAGTLRRSDMDAELERAYRAGLEQALEAGHAVLRGGGAALDAVCAAVCALEDDPLFNAGRGSVFNLDGKQEM
EAAVMDGIRQDAGSVAGLSGVRNPVLLARAVMERTPHVTLGYAAAEDFARRAGLEMRPPEYFHTDKRWQALLAEKERLAN
GGSDEDISEDRKHGTVGAVALDAQGRLAAATSTGGRTAKWPGRIGDTPVIGAGTWADAYCAVSATGHGEYFVRAAAGHEI
SARIRHLDETVAQACDAVVYGQLLTMGGTGGVVAVDRYGRVALPFNCEGMYRAAIDGAGLRTVAIYRGED

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=301, Percent_Identity=40.1993355481728, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI145275200, Length=301, Percent_Identity=40.1993355481728, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI98991769, Length=274, Percent_Identity=33.5766423357664, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI285002251, Length=211, Percent_Identity=30.3317535545024, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI285002253, Length=211, Percent_Identity=28.436018957346, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1787050, Length=307, Percent_Identity=48.8599348534202, Blast_Score=263, Evalue=1e-71,
Organism=Drosophila melanogaster, GI18921183, Length=305, Percent_Identity=37.7049180327869, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI21357893, Length=269, Percent_Identity=31.9702602230483, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24652135, Length=187, Percent_Identity=33.6898395721925, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI221330102, Length=187, Percent_Identity=33.6898395721925, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 32583; Mature: 32452

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIALALHGGAGTLRRSDMDAELERAYRAGLEQALEAGHAVLRGGGAALDAVCAAVCALE
CEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHEECCCCHHHHHHHHHHHHCC
DDPLFNAGRGSVFNLDGKQEMEAAVMDGIRQDAGSVAGLSGVRNPVLLARAVMERTPHVT
CCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHCCCCEE
LGYAAAEDFARRAGLEMRPPEYFHTDKRWQALLAEKERLANGGSDEDISEDRKHGTVGAV
EEHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCEEEE
ALDAQGRLAAATSTGGRTAKWPGRIGDTPVIGAGTWADAYCAVSATGHGEYFVRAAAGHE
EECCCCCEEEEECCCCCCCCCCCCCCCCCEECCCCCCCCEEEEEECCCCCEEEEECCCCH
ISARIRHLDETVAQACDAVVYGQLLTMGGTGGVVAVDRYGRVALPFNCEGMYRAAIDGAG
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCEEEEECCCCCEEEEECCCC
LRTVAIYRGED
CEEEEEEECCC
>Mature Secondary Structure 
TIALALHGGAGTLRRSDMDAELERAYRAGLEQALEAGHAVLRGGGAALDAVCAAVCALE
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHEECCCCHHHHHHHHHHHHCC
DDPLFNAGRGSVFNLDGKQEMEAAVMDGIRQDAGSVAGLSGVRNPVLLARAVMERTPHVT
CCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHCCCCEE
LGYAAAEDFARRAGLEMRPPEYFHTDKRWQALLAEKERLANGGSDEDISEDRKHGTVGAV
EEHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCEEEE
ALDAQGRLAAATSTGGRTAKWPGRIGDTPVIGAGTWADAYCAVSATGHGEYFVRAAAGHE
EECCCCCEEEEECCCCCCCCCCCCCCCCCEECCCCCCCCEEEEEECCCCCEEEEECCCCH
ISARIRHLDETVAQACDAVVYGQLLTMGGTGGVVAVDRYGRVALPFNCEGMYRAAIDGAG
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCEEEEECCCCCEEEEECCCC
LRTVAIYRGED
CEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]