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The map label for this gene is rhtB [H]

Identifier: 34499499

GI number: 34499499

Start: 4376250

End: 4376864

Strand: Direct

Name: rhtB [H]

Synonym: CV_4044

Alternate gene names: 34499499

Gene position: 4376250-4376864 (Clockwise)

Preceding gene: 34499498

Following gene: 34499502

Centisome position: 92.11

GC content: 67.32

Gene sequence:

>615_bases
ATGCACCATTACCCGCTGTTCCTGCTGATGGCCAGCCTGGCCATCCTGACGCCCGGCCCCGGCGTGCTCAACTCGCTGGG
CAACGCGCTGCGTTTCGGCCTCAGGGGCAGCCTGCCCGGCATACTGGGCGTCGCCTGCGGCAGCTTCATCGTCGCCGCGC
TGTCCGCCACCGGCCTGGGCCTGCTGCTGGCGACCTCCGCCCTGGCTTTCACCGCGCTGAAATGGCTGGGCGCCGGCTAC
CTGTTCTACCTGGGCATCAAGCTGCTGCGGGCGCCGGCCTTCCGTTTCGACGCGCCGGAAGCCAAGCGTACCAGCCCCTG
GCGGCAGTTCGCCGCCGGCATGCTGCTGCAGTTGAGCAACCCCAAGTCCATCTTTTTCTTCCTGGCCATCTTCCCGCAGT
TCATCGCCGCCGGCAGCGCCCATTACACCAGCGACTTCCTGCTGCTGGTCAGCAGCTACAGCGCGCTGCTGGTGCTGATC
CACAGCGCCTACGCCTTGCTGGCTCACTCGTTGCGGCGCTGGCTGGACAACGAGCGCGGCGGCCACCTGATCAACAAGGC
CAGCGGCGCCAGCTTCCTCGTGTTCGGCTCTCTGCTGGCCACGTCCAACCGCTAA

Upstream 100 bases:

>100_bases
CGCCAGGCCGGCTTCTGGCTGGGCCGCTATCAGGACCTGGTCAATCTGTCGCTGCTGCGCGAGGAAAGCGCGCTGGCCGC
GGCAATCTAGGAGAAAACCG

Downstream 100 bases:

>100_bases
GTTTTACCAGCCGTTTTCCAGCACGATATGGCCCGGCGCGTTCTGCCGGGTCATCCGGTAGCCCATCTTCATCAGTTGGC
GGTGGGTATCCTCCACCATT

Product: amino acid transporter LysE

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MHHYPLFLLMASLAILTPGPGVLNSLGNALRFGLRGSLPGILGVACGSFIVAALSATGLGLLLATSALAFTALKWLGAGY
LFYLGIKLLRAPAFRFDAPEAKRTSPWRQFAAGMLLQLSNPKSIFFFLAIFPQFIAAGSAHYTSDFLLLVSSYSALLVLI
HSAYALLAHSLRRWLDNERGGHLINKASGASFLVFGSLLATSNR

Sequences:

>Translated_204_residues
MHHYPLFLLMASLAILTPGPGVLNSLGNALRFGLRGSLPGILGVACGSFIVAALSATGLGLLLATSALAFTALKWLGAGY
LFYLGIKLLRAPAFRFDAPEAKRTSPWRQFAAGMLLQLSNPKSIFFFLAIFPQFIAAGSAHYTSDFLLLVSSYSALLVLI
HSAYALLAHSLRRWLDNERGGHLINKASGASFLVFGSLLATSNR
>Mature_204_residues
MHHYPLFLLMASLAILTPGPGVLNSLGNALRFGLRGSLPGILGVACGSFIVAALSATGLGLLLATSALAFTALKWLGAGY
LFYLGIKLLRAPAFRFDAPEAKRTSPWRQFAAGMLLQLSNPKSIFFFLAIFPQFIAAGSAHYTSDFLLLVSSYSALLVLI
HSAYALLAHSLRRWLDNERGGHLINKASGASFLVFGSLLATSNR

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=198, Percent_Identity=34.3434343434343, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1788099, Length=206, Percent_Identity=31.0679611650485, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI48994978, Length=197, Percent_Identity=27.9187817258883, Blast_Score=67, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21827; Mature: 21827

Theoretical pI: Translated: 10.86; Mature: 10.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHHYPLFLLMASLAILTPGPGVLNSLGNALRFGLRGSLPGILGVACGSFIVAALSATGLG
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LLLATSALAFTALKWLGAGYLFYLGIKLLRAPAFRFDAPEAKRTSPWRQFAAGMLLQLSN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
PKSIFFFLAIFPQFIAAGSAHYTSDFLLLVSSYSALLVLIHSAYALLAHSLRRWLDNERG
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GHLINKASGASFLVFGSLLATSNR
CCEEECCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MHHYPLFLLMASLAILTPGPGVLNSLGNALRFGLRGSLPGILGVACGSFIVAALSATGLG
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LLLATSALAFTALKWLGAGYLFYLGIKLLRAPAFRFDAPEAKRTSPWRQFAAGMLLQLSN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
PKSIFFFLAIFPQFIAAGSAHYTSDFLLLVSSYSALLVLIHSAYALLAHSLRRWLDNERG
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GHLINKASGASFLVFGSLLATSNR
CCEEECCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]