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The map label for this gene is capH [H]

Identifier: 34499479

GI number: 34499479

Start: 4350230

End: 4350865

Strand: Reverse

Name: capH [H]

Synonym: CV_4024

Alternate gene names: 34499479

Gene position: 4350865-4350230 (Counterclockwise)

Preceding gene: 34499480

Following gene: 34499478

Centisome position: 91.58

GC content: 40.09

Gene sequence:

>636_bases
TTGTATTTCTTATGGTTTTTTTTGATTAAAAAACTTAAATACCCGAGCTGTAATATTGAGACGAATTTTATACTGCCAGG
CGTAAAACTTGGACGCGGTGTCATTGTAAGAAAGAATGTAAAGATTTACCGGAACGTAAGTATCGGAGATCATACATTTA
TTAATGAGGATAGCCGGATCGATCCTAATACGGAGAGCATTGGTAAGTTTTGCTCCATATCACATGGCGTGAAAATAGGG
CTTGGTCCGCATCCACTACTTTTCTTCTCCACAAGCCCACTTTTTTATCAACGTTATCGTGGCTTGGTTGGGAGTGATTT
TTATGATGAATTTGCCGATAAAGGATATACGAGAATTGGTCATGATGTTTTTATCGCGGCAAATGCAGTCATCGTTTCAG
GTGTTGAAATAGGCCATGGCGCGGTAGTTGCAGCTGGCAGCGTCGTGACTAAAAATGTTCCTCCATATGCTGTTGTGGGT
GGTGTGCCTGCACGAATCATCAAGTATCGATTCGATGAGGATACAATTGAAAAGCTACTGAAAAGTAAGTGGTGGAATAA
AGATATCAATATTTTGGCGCGTAACGCTGCTAATGGGTTTGATATAGATGTCTTCTTGGATTCGTTAGAGGAATAA

Upstream 100 bases:

>100_bases
TCGAGTCGTGTACCTGATAAGTTTGTCTTAATTGGGGGGGCGACAGATTTGATCGATCAGATAGGGCATTGCGATGTTAA
TAAAAGCATTCATTCATAAT

Downstream 100 bases:

>100_bases
ATGACTTTAAAAGTTAAGATCATTAAAGATGTCAATAAAGTCAATGGGGATTTTGAGTCGCATGTGTTTGCAAGCGAAGG
CTATTTGAAGGCATGTTCTG

Product: capsular polysaccharide synthesis enzyme O-acetyl transferase

Products: CoA; acetylmaltose [C]

Alternate protein names: Virginiamycin acetyltransferase D; Vat(D) [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MYFLWFFLIKKLKYPSCNIETNFILPGVKLGRGVIVRKNVKIYRNVSIGDHTFINEDSRIDPNTESIGKFCSISHGVKIG
LGPHPLLFFSTSPLFYQRYRGLVGSDFYDEFADKGYTRIGHDVFIAANAVIVSGVEIGHGAVVAAGSVVTKNVPPYAVVG
GVPARIIKYRFDEDTIEKLLKSKWWNKDINILARNAANGFDIDVFLDSLEE

Sequences:

>Translated_211_residues
MYFLWFFLIKKLKYPSCNIETNFILPGVKLGRGVIVRKNVKIYRNVSIGDHTFINEDSRIDPNTESIGKFCSISHGVKIG
LGPHPLLFFSTSPLFYQRYRGLVGSDFYDEFADKGYTRIGHDVFIAANAVIVSGVEIGHGAVVAAGSVVTKNVPPYAVVG
GVPARIIKYRFDEDTIEKLLKSKWWNKDINILARNAANGFDIDVFLDSLEE
>Mature_211_residues
MYFLWFFLIKKLKYPSCNIETNFILPGVKLGRGVIVRKNVKIYRNVSIGDHTFINEDSRIDPNTESIGKFCSISHGVKIG
LGPHPLLFFSTSPLFYQRYRGLVGSDFYDEFADKGYTRIGHDVFIAANAVIVSGVEIGHGAVVAAGSVVTKNVPPYAVVG
GVPARIIKYRFDEDTIEKLLKSKWWNKDINILARNAANGFDIDVFLDSLEE

Specific function: Inactivates the A compounds of streptogramin antibiotics by acetylation, thus providing resistance to these antibiotics [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 23623; Mature: 23623

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYFLWFFLIKKLKYPSCNIETNFILPGVKLGRGVIVRKNVKIYRNVSIGDHTFINEDSRI
CCHHHHHHHHHHCCCCCCCEEEEEECCHHHCCEEEEECCEEEEEECCCCCCEEECCCCCC
DPNTESIGKFCSISHGVKIGLGPHPLLFFSTSPLFYQRYRGLVGSDFYDEFADKGYTRIG
CCCHHHHHHHHHHCCCEEECCCCCCEEEEECCHHHHHHHCCCCCCHHHHHHHHCCCCCCC
HDVFIAANAVIVSGVEIGHGAVVAAGSVVTKNVPPYAVVGGVPARIIKYRFDEDTIEKLL
CEEEEEECEEEEECEEECCCEEEEECCEEECCCCCEEEECCCCHHEEEEECCHHHHHHHH
KSKWWNKDINILARNAANGFDIDVFLDSLEE
HHHCCCCCCEEEEECCCCCCCCEEEHHHHCC
>Mature Secondary Structure
MYFLWFFLIKKLKYPSCNIETNFILPGVKLGRGVIVRKNVKIYRNVSIGDHTFINEDSRI
CCHHHHHHHHHHCCCCCCCEEEEEECCHHHCCEEEEECCEEEEEECCCCCCEEECCCCCC
DPNTESIGKFCSISHGVKIGLGPHPLLFFSTSPLFYQRYRGLVGSDFYDEFADKGYTRIG
CCCHHHHHHHHHHCCCEEECCCCCCEEEEECCHHHHHHHCCCCCCHHHHHHHHCCCCCCC
HDVFIAANAVIVSGVEIGHGAVVAAGSVVTKNVPPYAVVGGVPARIIKYRFDEDTIEKLL
CEEEEEECEEEEECEEECCCEEEEECCEEECCCCCEEEECCCCHHEEEEECCHHHHHHHH
KSKWWNKDINILARNAANGFDIDVFLDSLEE
HHHCCCCCCEEEEECCCCCCCCEEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 90 {maltose}} 62 {glucose}} 0.018 {acetyl-CoA}} 40 {triethanolamine}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8257133 [H]