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The map label for this gene is rfbD [H]

Identifier: 34499466

GI number: 34499466

Start: 4337472

End: 4338404

Strand: Direct

Name: rfbD [H]

Synonym: CV_4011

Alternate gene names: 34499466

Gene position: 4337472-4338404 (Clockwise)

Preceding gene: 34499465

Following gene: 34499467

Centisome position: 91.29

GC content: 65.27

Gene sequence:

>933_bases
ATGATGACCATGCCCCGCATACTGATAACCGGCAAAAACGGCCAGGTCGGCCACGAATTGCTGCGCGCGCTGGCGCCGCT
GGGCGAGCTGATCGCCACCGACAGGCGGGAGCTGGACCTCACGTGGGACGACGCGCGCATCGAGCAGGCGCTGGACCAGC
ATGGGCCCGACATCATCGTCAACCCGGCCGCCTATACCGCGGTCGACAAGGCGGAAAGCGACGAGGCAACGGCGCACGCC
GTCAACGCCTGCGCGGTCGGCGTCATCGCGCGCTGGACCGCCCGCCATGGCGCGTTGCTTGTCCACTACTCCACCGACTA
CGTGTTCAGCGGAGACGGCGAACGCCCCTGGCGGGAAGACGACGCAACCGGCCCGCAATCGGTATATGGACGCAGCAAGC
TGGCGGGCGAAGAAGCCATCCGGCATGCCGCGCCTCGCCACCTGATACTCCGCACCAGCTGGGTTTTCGGCGCGCATGGC
GCCAATTTCCTGAAAACCATGCTGAAGCTGGGACAGGAACGGGATTCGCTCAAGGTCGTCAACGACCAGATCGGCGCGCC
AGCCTCCGCCGCCATGATCGCCGACGTCAGCGCCGAACTGATTCGCCGCTACCTGGCCGCCCCCTCCGGCTTCGCCTTTG
GAACCTACCATCTGGCGCCCCGGGGGGAAACCAGCTGGCACGGCTATGCAAGCTATCTGCTGCAGCGAGCCGCGTCCTAC
GGACTACCACTGAAAATCGATCCCGCAAACATCCAGGGCATTCCCAGCAGCGACTATCCGCTGCCAGCCAAGCGCCCAGC
CAACTCCAGGCTGAGTTGCGACAAGCTGAAAAGCGCTTTCGGCATCGAGCTGCCGGAATGGAGAAGCGGCGTGGACCAGG
TGCTGGCTCAATTGCTCCCCACCTACGACAGCGGTGCCCCAGCCCGGGAATGA

Upstream 100 bases:

>100_bases
GCGGCATCCGCAAGACCATTGAATGGTATCTGGGCAATCCAAACTGGATCGAACACGTCAGCAACGGCAGCTACCGCGAC
TGGCTGGACAAGCACTACGG

Downstream 100 bases:

>100_bases
CCACAGGAAATCTGGCAATGAAACGAAAAGGCATCATTCTGGCCGGAGGCTCCGGCACCCGGCTCTACCCGGCCACCATC
GCCGTCAGCAAGCAATTGCT

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MMTMPRILITGKNGQVGHELLRALAPLGELIATDRRELDLTWDDARIEQALDQHGPDIIVNPAAYTAVDKAESDEATAHA
VNACAVGVIARWTARHGALLVHYSTDYVFSGDGERPWREDDATGPQSVYGRSKLAGEEAIRHAAPRHLILRTSWVFGAHG
ANFLKTMLKLGQERDSLKVVNDQIGAPASAAMIADVSAELIRRYLAAPSGFAFGTYHLAPRGETSWHGYASYLLQRAASY
GLPLKIDPANIQGIPSSDYPLPAKRPANSRLSCDKLKSAFGIELPEWRSGVDQVLAQLLPTYDSGAPARE

Sequences:

>Translated_310_residues
MMTMPRILITGKNGQVGHELLRALAPLGELIATDRRELDLTWDDARIEQALDQHGPDIIVNPAAYTAVDKAESDEATAHA
VNACAVGVIARWTARHGALLVHYSTDYVFSGDGERPWREDDATGPQSVYGRSKLAGEEAIRHAAPRHLILRTSWVFGAHG
ANFLKTMLKLGQERDSLKVVNDQIGAPASAAMIADVSAELIRRYLAAPSGFAFGTYHLAPRGETSWHGYASYLLQRAASY
GLPLKIDPANIQGIPSSDYPLPAKRPANSRLSCDKLKSAFGIELPEWRSGVDQVLAQLLPTYDSGAPARE
>Mature_310_residues
MMTMPRILITGKNGQVGHELLRALAPLGELIATDRRELDLTWDDARIEQALDQHGPDIIVNPAAYTAVDKAESDEATAHA
VNACAVGVIARWTARHGALLVHYSTDYVFSGDGERPWREDDATGPQSVYGRSKLAGEEAIRHAAPRHLILRTSWVFGAHG
ANFLKTMLKLGQERDSLKVVNDQIGAPASAAMIADVSAELIRRYLAAPSGFAFGTYHLAPRGETSWHGYASYLLQRAASY
GLPLKIDPANIQGIPSSDYPLPAKRPANSRLSCDKLKSAFGIELPEWRSGVDQVLAQLLPTYDSGAPARE

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=307, Percent_Identity=26.3843648208469, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI33519455, Length=307, Percent_Identity=26.3843648208469, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1788352, Length=299, Percent_Identity=47.4916387959866, Blast_Score=278, Evalue=3e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 33687; Mature: 33687

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTMPRILITGKNGQVGHELLRALAPLGELIATDRRELDLTWDDARIEQALDQHGPDIIV
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHCCCCEEE
NPAAYTAVDKAESDEATAHAVNACAVGVIARWTARHGALLVHYSTDYVFSGDGERPWRED
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCCC
DATGPQSVYGRSKLAGEEAIRHAAPRHLILRTSWVFGAHGANFLKTMLKLGQERDSLKVV
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHCCCCCHHHHH
NDQIGAPASAAMIADVSAELIRRYLAAPSGFAFGTYHLAPRGETSWHGYASYLLQRAASY
HHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHC
GLPLKIDPANIQGIPSSDYPLPAKRPANSRLSCDKLKSAFGIELPEWRSGVDQVLAQLLP
CCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCC
TYDSGAPARE
CCCCCCCCCC
>Mature Secondary Structure
MMTMPRILITGKNGQVGHELLRALAPLGELIATDRRELDLTWDDARIEQALDQHGPDIIV
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHCCCCEEE
NPAAYTAVDKAESDEATAHAVNACAVGVIARWTARHGALLVHYSTDYVFSGDGERPWRED
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCCC
DATGPQSVYGRSKLAGEEAIRHAAPRHLILRTSWVFGAHGANFLKTMLKLGQERDSLKVV
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHCCCCCHHHHH
NDQIGAPASAAMIADVSAELIRRYLAAPSGFAFGTYHLAPRGETSWHGYASYLLQRAASY
HHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHC
GLPLKIDPANIQGIPSSDYPLPAKRPANSRLSCDKLKSAFGIELPEWRSGVDQVLAQLLP
CCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCC
TYDSGAPARE
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]