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The map label for this gene is cyoE

Identifier: 34499447

GI number: 34499447

Start: 4320113

End: 4321021

Strand: Reverse

Name: cyoE

Synonym: CV_3992

Alternate gene names: 34499447

Gene position: 4321021-4320113 (Counterclockwise)

Preceding gene: 34499448

Following gene: 34499436

Centisome position: 90.95

GC content: 66.01

Gene sequence:

>909_bases
TTGAAGTTCAAGCGTTATCTTCAGGTCACTAAGCCCGGCATCATTTTCGGCAACCTGATCTCGGCCGCCGGCGGTTTCCT
GCTGGCGGCGCAGGGATCGGTCGACTGGTGGCTGCTCGCGGCCACCGTCGTCGGCTTGTCGCTGGTGGTGGCCTCCGGCT
GCGCGATCAACAACTGCATCGATCAGGACATCGACGCCAAGATGGCCCGCACCCAGAAACGGGTGCTGGTCACCGGCGCG
ATGAGCACCAAGGCCGCGTTGGCGCATGGCGTGGTGCTGGGCCTGGCGGGCTTCGCCCTGCTGTGGTTCTGCACCAATCC
GCTGGCCACCGGCTGTGTGCTGTTCGGCTTTGTGATCTATGTCGGCGTGTACAGCCTGTACATGAAGCGCAACTCGGTGT
ACGGCACCCTGGTGGGCAGCCTGTCCGGCGCGGTGCCGCCGGTGGCCGGCTACTGCGCCGTCACCGGCCGCTTCGACATG
GGCGCGGCCATCCTGCTGCTGATGTTCAGCCTGTGGCAGATGCCGCACTCGTACGCGATCGCCATCTTCCGCTTCAAGGA
CTACGAGGCGGCCGGCATCCCGGTGCTGCCGGTGGTGAAGGGCATCTCCGCCGCCAAGCAGCAGATCGTGCTCTACATCC
TGGCCTTCGCTGCGGCGACCGTGATGCTGGTATTCGGCGGCTACGCCGGCTACGGCTACCTGGCGGTGGCGGTGGCCACC
AGCCTGTGGTGGCTGAAGATGGCGCTGTCCGGTTACAAGCGCGAAACCGACGACCGCGCCTGGGCGCGCCAGGTGTTCTT
CTTCTCCATCATCACCATCACCTCGCTGAGCGTGATGATGGCGGTGGACGGGCAGACGCCGCCGCACAGCCGCGCGGTGA
TGACCGCGGACGCGGGCCACTACGGCTAA

Upstream 100 bases:

>100_bases
CCTTCCTGTTCACCGGCATGGTGATCGTGCTGCTGGTGGTCCTGTCGATCTGGATCATCTACACCGCCGACGCGCTGATG
ATGCGCTGAGCGAGGCTGAG

Downstream 100 bases:

>100_bases
GATCGACACCATCCCATGGCAAACGGGGAGCTGCGCAAGCGGCTCCCCGTTTTTGTTTGGCGTCGCTCAGCGCGGCGGCC
AGGCGGCATCTTGCCGGCCG

Product: protoheme IX farnesyltransferase

Products: diphosphate; presqualene diphosphate

Alternate protein names: Heme B farnesyltransferase 2; Heme O synthase 2

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKFKRYLQVTKPGIIFGNLISAAGGFLLAAQGSVDWWLLAATVVGLSLVVASGCAINNCIDQDIDAKMARTQKRVLVTGA
MSTKAALAHGVVLGLAGFALLWFCTNPLATGCVLFGFVIYVGVYSLYMKRNSVYGTLVGSLSGAVPPVAGYCAVTGRFDM
GAAILLLMFSLWQMPHSYAIAIFRFKDYEAAGIPVLPVVKGISAAKQQIVLYILAFAAATVMLVFGGYAGYGYLAVAVAT
SLWWLKMALSGYKRETDDRAWARQVFFFSIITITSLSVMMAVDGQTPPHSRAVMTADAGHYG

Sequences:

>Translated_302_residues
MKFKRYLQVTKPGIIFGNLISAAGGFLLAAQGSVDWWLLAATVVGLSLVVASGCAINNCIDQDIDAKMARTQKRVLVTGA
MSTKAALAHGVVLGLAGFALLWFCTNPLATGCVLFGFVIYVGVYSLYMKRNSVYGTLVGSLSGAVPPVAGYCAVTGRFDM
GAAILLLMFSLWQMPHSYAIAIFRFKDYEAAGIPVLPVVKGISAAKQQIVLYILAFAAATVMLVFGGYAGYGYLAVAVAT
SLWWLKMALSGYKRETDDRAWARQVFFFSIITITSLSVMMAVDGQTPPHSRAVMTADAGHYG
>Mature_302_residues
MKFKRYLQVTKPGIIFGNLISAAGGFLLAAQGSVDWWLLAATVVGLSLVVASGCAINNCIDQDIDAKMARTQKRVLVTGA
MSTKAALAHGVVLGLAGFALLWFCTNPLATGCVLFGFVIYVGVYSLYMKRNSVYGTLVGSLSGAVPPVAGYCAVTGRFDM
GAAILLLMFSLWQMPHSYAIAIFRFKDYEAAGIPVLPVVKGISAAKQQIVLYILAFAAATVMLVFGGYAGYGYLAVAVAT
SLWWLKMALSGYKRETDDRAWARQVFFFSIITITSLSVMMAVDGQTPPHSRAVMTADAGHYG

Specific function: Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group

COG id: COG0109

COG function: function code O; Polyprenyltransferase (cytochrome oxidase assembly factor)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily

Homologues:

Organism=Homo sapiens, GI17921982, Length=172, Percent_Identity=35.4651162790698, Blast_Score=95, Evalue=9e-20,
Organism=Escherichia coli, GI1786631, Length=287, Percent_Identity=70.7317073170732, Blast_Score=401, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI71998235, Length=195, Percent_Identity=35.8974358974359, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6325085, Length=220, Percent_Identity=28.6363636363636, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI19921064, Length=268, Percent_Identity=28.7313432835821, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COXX2_CHRVO (Q7NQZ3)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903662.1
- GeneID:   2549973
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3992
- NMPDR:   fig|243365.1.peg.3992
- HOGENOM:   HBG656003
- OMA:   VGYCAVT
- PhylomeDB:   Q7NQZ3
- ProtClustDB:   PRK04375
- BioCyc:   CVIO243365:CV_3992-MONOMER
- HAMAP:   MF_00154
- InterPro:   IPR006369
- InterPro:   IPR000537
- PANTHER:   PTHR11048:SF3
- TIGRFAMs:   TIGR01473

Pfam domain/function: PF01040 UbiA

EC number: 2.5.1.21

Molecular weight: Translated: 32277; Mature: 32277

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00943 UBIA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1344df68)-; HASH(0x13fee91c)-; HASH(0x13feea30)-; HASH(0x13feec1c)-; HASH(0x13fccaec)-; HASH(0x13883bd8)-; HASH(0x13ee1654)-; HASH(0x13c68594)-; HASH(0x13fee9b8)-;

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKRYLQVTKPGIIFGNLISAAGGFLLAAQGSVDWWLLAATVVGLSLVVASGCAINNCI
CCHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
DQDIDAKMARTQKRVLVTGAMSTKAALAHGVVLGLAGFALLWFCTNPLATGCVLFGFVIY
CCCHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VGVYSLYMKRNSVYGTLVGSLSGAVPPVAGYCAVTGRFDMGAAILLLMFSLWQMPHSYAI
HHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEE
AIFRFKDYEAAGIPVLPVVKGISAAKQQIVLYILAFAAATVMLVFGGYAGYGYLAVAVAT
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SLWWLKMALSGYKRETDDRAWARQVFFFSIITITSLSVMMAVDGQTPPHSRAVMTADAGH
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEECCCC
YG
CH
>Mature Secondary Structure
MKFKRYLQVTKPGIIFGNLISAAGGFLLAAQGSVDWWLLAATVVGLSLVVASGCAINNCI
CCHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
DQDIDAKMARTQKRVLVTGAMSTKAALAHGVVLGLAGFALLWFCTNPLATGCVLFGFVIY
CCCHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VGVYSLYMKRNSVYGTLVGSLSGAVPPVAGYCAVTGRFDMGAAILLLMFSLWQMPHSYAI
HHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEE
AIFRFKDYEAAGIPVLPVVKGISAAKQQIVLYILAFAAATVMLVFGGYAGYGYLAVAVAT
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SLWWLKMALSGYKRETDDRAWARQVFFFSIITITSLSVMMAVDGQTPPHSRAVMTADAGH
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEECCCC
YG
CH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: farnesyl diphosphate

Specific reaction: 2 farnesyl diphosphate = diphosphate + presqualene diphosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 14500782