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The map label for this gene is fabF [C]

Identifier: 34499404

GI number: 34499404

Start: 4270200

End: 4271429

Strand: Reverse

Name: fabF [C]

Synonym: CV_3949

Alternate gene names: 34499404

Gene position: 4271429-4270200 (Counterclockwise)

Preceding gene: 34499405

Following gene: 34499403

Centisome position: 89.9

GC content: 71.63

Gene sequence:

>1230_bases
ATGGACAAGCGAAGAGTGGTGATCACCGGCATCGGCGTGGTGTCGCCTTACGGCGCCGGCCTGCAGCGTTTCTGGCAGGC
GCTGGCGGGCGGCGAGAGCGCGCGGCGGCCGATACAAGGCTTCGACGCCTCGGTTTACCGCAGCGCCCAGGGCGGCGAGG
CGCCGGCGGAGCTGCTGGCCGACGCCGGCGCGCGCGCGCGCTGCGGCGATCCGGGCGAGGACAGCGCCTACTTCCTGGCG
CTGGCCGCCGCCGAGGCGTTGGCGGATGCCGGCCTGGGCGCGGCGTTCTCCGACGAAGACCGGGTGGGCTGCGTGCTGGG
CACGCTGTGCGCCGGCATGCGCAATATGCGCGAATACGGCGCCGCTTACGAGGCGGGGCGGCCGTCGCCGGTGGCGGCGG
AGACGATCCCGGCCCATTACCAGCTGGACTTCCTGGCGCGGCGGCACAATCTGACCGGGCCGTCGTCGCTGGTGTCCACC
GCCTGCTCGTCCACCACCGACGCGTTGGGCTATGCCTTCGACCTGATACGCGGCGGCGAATGCGATAGCTGCCTGTCCGG
CGGCGGCGACGTGCTGGCGGAGATGATACACGCGGCCTTCAACGGCCTGTATTCGATCACCACGGGCGAGCCCAGGCCGT
TCGACAGCGAGCGGGACGGTTTCTTCATCGGCGAGGGCGCCGGTCTGTTCCATCTGGAAACGCTGGAGTCGGCGCGCGCG
CGCGGCGCGCGCATCCATGCCGAGATTTTGGGCTACGGCCTGTCCAACACCGGCCACCATCTGACCGCCACCTCGGAAGA
CGGCGCCGGCGAGGCGCTGGCGATACGCCGCGCGCTGGACGACGCCGGCCTGGCGCCGGCGGACATCGACTACATCAACT
GCCACGGCACCGGCACCCGCCACAACGACGCGTCCGAGATCCGCGCGATCAACCGGGTGTTCGGCGACGCCGCCGAGCGG
CTGCACATCAGCTCCAACAAGGCCTCGATCGGTCATTGCATGGGCGCGGCCGGCGCGCTGGAGGCGGCCGCCACGGTGCT
GGCGCTGGAGAAGGGCCTGATTCCGCCGACGCTGCACACCCGCGGCGACGAGGCGGAGTTGCGCAGTCGCCTGGTCGGGG
CCGAGGCCCGGCCCGCTGCGTTGCGGCGCGCGCTGTCGCAGTCGTTCGGCTTCGGCGGCGCCTGCTCCTGCATCGTGTTC
GGCCATTATGGGGAGGATGGGCATGCGTGA

Upstream 100 bases:

>100_bases
TACGCGGTCAGCGGGCTGGGCAAGGTGGACGGGGACAAGGTGCTGACCGTGGAATTGACGATAGCCGGCGTGCGGCGCTA
GCGCGGCGGCGGGAGGGGGC

Downstream 100 bases:

>100_bases
AGTGGCGATTACCGGCATCGGCGTTGTCGGGCCGCACGGCGTGGGGCTGGCTGGCGTGCTCGACGGGCGCTTCGTGTTTT
CCGCCTGGCCGGATTCGGCG

Product: 3-oxoacyl-ACP synthase II

Products: 3-oxoacyl-[acyl-carrier protein]; CO2; [acyl-carrier protein]

Alternate protein names: actI ORF1 [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MDKRRVVITGIGVVSPYGAGLQRFWQALAGGESARRPIQGFDASVYRSAQGGEAPAELLADAGARARCGDPGEDSAYFLA
LAAAEALADAGLGAAFSDEDRVGCVLGTLCAGMRNMREYGAAYEAGRPSPVAAETIPAHYQLDFLARRHNLTGPSSLVST
ACSSTTDALGYAFDLIRGGECDSCLSGGGDVLAEMIHAAFNGLYSITTGEPRPFDSERDGFFIGEGAGLFHLETLESARA
RGARIHAEILGYGLSNTGHHLTATSEDGAGEALAIRRALDDAGLAPADIDYINCHGTGTRHNDASEIRAINRVFGDAAER
LHISSNKASIGHCMGAAGALEAAATVLALEKGLIPPTLHTRGDEAELRSRLVGAEARPAALRRALSQSFGFGGACSCIVF
GHYGEDGHA

Sequences:

>Translated_409_residues
MDKRRVVITGIGVVSPYGAGLQRFWQALAGGESARRPIQGFDASVYRSAQGGEAPAELLADAGARARCGDPGEDSAYFLA
LAAAEALADAGLGAAFSDEDRVGCVLGTLCAGMRNMREYGAAYEAGRPSPVAAETIPAHYQLDFLARRHNLTGPSSLVST
ACSSTTDALGYAFDLIRGGECDSCLSGGGDVLAEMIHAAFNGLYSITTGEPRPFDSERDGFFIGEGAGLFHLETLESARA
RGARIHAEILGYGLSNTGHHLTATSEDGAGEALAIRRALDDAGLAPADIDYINCHGTGTRHNDASEIRAINRVFGDAAER
LHISSNKASIGHCMGAAGALEAAATVLALEKGLIPPTLHTRGDEAELRSRLVGAEARPAALRRALSQSFGFGGACSCIVF
GHYGEDGHA
>Mature_409_residues
MDKRRVVITGIGVVSPYGAGLQRFWQALAGGESARRPIQGFDASVYRSAQGGEAPAELLADAGARARCGDPGEDSAYFLA
LAAAEALADAGLGAAFSDEDRVGCVLGTLCAGMRNMREYGAAYEAGRPSPVAAETIPAHYQLDFLARRHNLTGPSSLVST
ACSSTTDALGYAFDLIRGGECDSCLSGGGDVLAEMIHAAFNGLYSITTGEPRPFDSERDGFFIGEGAGLFHLETLESARA
RGARIHAEILGYGLSNTGHHLTATSEDGAGEALAIRRALDDAGLAPADIDYINCHGTGTRHNDASEIRAINRVFGDAAER
LHISSNKASIGHCMGAAGALEAAATVLALEKGLIPPTLHTRGDEAELRSRLVGAEARPAALRRALSQSFGFGGACSCIVF
GHYGEDGHA

Specific function: Catalyzes The Condensation Reaction Of Fatty Acid Synthesis By The Addition To An Acyl Acceptor Of Two Carbons From Malonyl-Acp. Has A Preference For Short Chain Acid Substrates And May Function To Supply The Octanoic Substrates For Lipoic Acid Biosynthe

COG id: COG0304

COG function: function code IQ; 3-oxoacyl-(acyl-carrier-protein) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-ketoacyl-ACP synthases family [H]

Homologues:

Organism=Homo sapiens, GI8923559, Length=421, Percent_Identity=35.8669833729216, Blast_Score=201, Evalue=8e-52,
Organism=Homo sapiens, GI41872631, Length=413, Percent_Identity=27.8450363196126, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI223972627, Length=144, Percent_Identity=42.3611111111111, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1787337, Length=411, Percent_Identity=34.0632603406326, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1788663, Length=413, Percent_Identity=31.4769975786925, Blast_Score=156, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71983541, Length=412, Percent_Identity=30.3398058252427, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71983535, Length=255, Percent_Identity=34.1176470588235, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI212642053, Length=333, Percent_Identity=30.03003003003, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17550940, Length=338, Percent_Identity=25.7396449704142, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320904, Length=442, Percent_Identity=32.3529411764706, Blast_Score=199, Evalue=5e-52,
Organism=Drosophila melanogaster, GI24644341, Length=423, Percent_Identity=37.1158392434988, Blast_Score=212, Evalue=3e-55,
Organism=Drosophila melanogaster, GI221330659, Length=356, Percent_Identity=29.2134831460674, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19920632, Length=356, Percent_Identity=29.2134831460674, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24581345, Length=264, Percent_Identity=28.7878787878788, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000794
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C [H]

EC number: 2.3.1.41

Molecular weight: Translated: 42481; Mature: 42481

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00606 B_KETOACYL_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKRRVVITGIGVVSPYGAGLQRFWQALAGGESARRPIQGFDASVYRSAQGGEAPAELLA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCCHHHHHCCCCCCCHHHHHH
DAGARARCGDPGEDSAYFLALAAAEALADAGLGAAFSDEDRVGCVLGTLCAGMRNMREYG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
AAYEAGRPSPVAAETIPAHYQLDFLARRHNLTGPSSLVSTACSSTTDALGYAFDLIRGGE
CHHCCCCCCCCCHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCC
CDSCLSGGGDVLAEMIHAAFNGLYSITTGEPRPFDSERDGFFIGEGAGLFHLETLESARA
CCHHHCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEEECCCCEEEHHHHHHHHH
RGARIHAEILGYGLSNTGHHLTATSEDGAGEALAIRRALDDAGLAPADIDYINCHGTGTR
CCCEEEHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
HNDASEIRAINRVFGDAAERLHISSNKASIGHCMGAAGALEAAATVLALEKGLIPPTLHT
CCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RGDEAELRSRLVGAEARPAALRRALSQSFGFGGACSCIVFGHYGEDGHA
CCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCC
>Mature Secondary Structure
MDKRRVVITGIGVVSPYGAGLQRFWQALAGGESARRPIQGFDASVYRSAQGGEAPAELLA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCCHHHHHCCCCCCCHHHHHH
DAGARARCGDPGEDSAYFLALAAAEALADAGLGAAFSDEDRVGCVLGTLCAGMRNMREYG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
AAYEAGRPSPVAAETIPAHYQLDFLARRHNLTGPSSLVSTACSSTTDALGYAFDLIRGGE
CHHCCCCCCCCCHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCC
CDSCLSGGGDVLAEMIHAAFNGLYSITTGEPRPFDSERDGFFIGEGAGLFHLETLESARA
CCHHHCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEEECCCCEEEHHHHHHHHH
RGARIHAEILGYGLSNTGHHLTATSEDGAGEALAIRRALDDAGLAPADIDYINCHGTGTR
CCCEEEHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
HNDASEIRAINRVFGDAAERLHISSNKASIGHCMGAAGALEAAATVLALEKGLIPPTLHT
CCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RGDEAELRSRLVGAEARPAALRRALSQSFGFGGACSCIVFGHYGEDGHA
CCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: None [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} [C]

Substrates: acyl-[acyl-carrier protein]; malonyl-[acyl-carrier protein]

Specific reaction: acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl-ACP-decarboxylation, Cerulenin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953; 1527048 [H]