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The map label for this gene is curA [H]

Identifier: 34499403

GI number: 34499403

Start: 4269038

End: 4270207

Strand: Reverse

Name: curA [H]

Synonym: CV_3948

Alternate gene names: 34499403

Gene position: 4270207-4269038 (Counterclockwise)

Preceding gene: 34499404

Following gene: 34499402

Centisome position: 89.88

GC content: 75.47

Gene sequence:

>1170_bases
ATGCGTGAAGTGGCGATTACCGGCATCGGCGTTGTCGGGCCGCACGGCGTGGGGCTGGCTGGCGTGCTCGACGGGCGCTT
CGTGTTTTCCGCCTGGCCGGATTCGGCGTCGCCGCCGCACGCCGAATCCCTGATCGCCAGCGTCGGCGACTACCCGCGCA
CCCGCTATTTCAGCGAGCGCGCGCTGCGGCTGATGGACAAGCCGATGATGCTGGCCAGCTTCGCCGCCGGCGCGGCGCTG
GAAGATGCCGGCTACGGCGACGGCGAGGCACCGGAGGACTGCGCGACGCTGCTGGGCACCGCGCGCTCGGAGCAGACCTC
GGTGCACAAGTTTTCGCTGCCGTTGCTGCAGGGGCGGCCGGAGCGGATCAATCCGGCCGAATTCCCGCTGGTGGCGCGCA
ATATCGCCTGCGGCCAGATGGCGATCCGCTTCGGCCTGCGGGGCCCCAGCTCGGTGCTGGCGTCGGGGCCGCTGGCTTCG
CTGCAGGCGGTGGCGCGCGCCGCCGGCCTGATCCGCTCCGGGCGAGCCAAGGTCGCGTTGGCGGGCGGCGTGGAGGCGTT
GTCGCGCTTCGCCTTGCGTTTCTGCCGCGATTACTACGGCGACGCGGCGCTGGCCGGCAGGCCGGCCTTCTTCGGCGAAC
GGGGCGGCGGGCTGATTCCGTCCGAGGGCGCGTGCATGCTGGTGCTGGAGGATGCCGAACACGCTGCGGCGCGCGGCGCG
CGCGTCTACGCCAGGCTGGGCGGCTGGCACGCGGGCCGGCTGGGCCGGGGCGACGCGGTCCAATCGCTGCTGGACGGCTG
GCGCCGCGCGGCCGACATGGCGCCGGCCCTGCTGCTGCCGGGCGCCGGCGGCGCGGGGCGCGCTCACGAAAGGCTGGAGA
CGCTGGCCTTGCGGCGCTGGCAGGCGGAGGGCGCCGCGCAGCCGTCCCGACTGTTAGCCGCGCGAGCCCTGGCGGGCGAG
GGCGAGAGCTGGAGCGGCGCGCTGCAGGTGGCCTTGGCCGCCGCCTCGCTGGCGCGCCGGCATGCGCCGGCGACGCCGGA
CGTGGCGGCTGACGCGGATCCGGCGCTGGCGCGGGCCGCCGCGGGCGGCACGCTGCCGGACGATGCCGCCGCGCTGGTGT
TGGGCAACGATGCCGCCGGCGCCTTCTGCGCGCTGGGGCTGCGCCGATGA

Upstream 100 bases:

>100_bases
GAGGCCCGGCCCGCTGCGTTGCGGCGCGCGCTGTCGCAGTCGTTCGGCTTCGGCGGCGCCTGCTCCTGCATCGTGTTCGG
CCATTATGGGGAGGATGGGC

Downstream 100 bases:

>100_bases
GCGTCATCATTTCCGCGCTGCGCGCTGGGAAGCGGCGGCCATATGGAGAATCCGATGAGTGAACACGCACTGCCGCTCGC
CTTGCGCAAGGCGGCGCTGG

Product: 3-oxoacyl-ACP synthase II

Products: 3-oxoacyl-[acyl-carrier protein]; CO2; [acyl-carrier protein]

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MREVAITGIGVVGPHGVGLAGVLDGRFVFSAWPDSASPPHAESLIASVGDYPRTRYFSERALRLMDKPMMLASFAAGAAL
EDAGYGDGEAPEDCATLLGTARSEQTSVHKFSLPLLQGRPERINPAEFPLVARNIACGQMAIRFGLRGPSSVLASGPLAS
LQAVARAAGLIRSGRAKVALAGGVEALSRFALRFCRDYYGDAALAGRPAFFGERGGGLIPSEGACMLVLEDAEHAAARGA
RVYARLGGWHAGRLGRGDAVQSLLDGWRRAADMAPALLLPGAGGAGRAHERLETLALRRWQAEGAAQPSRLLAARALAGE
GESWSGALQVALAAASLARRHAPATPDVAADADPALARAAAGGTLPDDAAALVLGNDAAGAFCALGLRR

Sequences:

>Translated_389_residues
MREVAITGIGVVGPHGVGLAGVLDGRFVFSAWPDSASPPHAESLIASVGDYPRTRYFSERALRLMDKPMMLASFAAGAAL
EDAGYGDGEAPEDCATLLGTARSEQTSVHKFSLPLLQGRPERINPAEFPLVARNIACGQMAIRFGLRGPSSVLASGPLAS
LQAVARAAGLIRSGRAKVALAGGVEALSRFALRFCRDYYGDAALAGRPAFFGERGGGLIPSEGACMLVLEDAEHAAARGA
RVYARLGGWHAGRLGRGDAVQSLLDGWRRAADMAPALLLPGAGGAGRAHERLETLALRRWQAEGAAQPSRLLAARALAGE
GESWSGALQVALAAASLARRHAPATPDVAADADPALARAAAGGTLPDDAAALVLGNDAAGAFCALGLRR
>Mature_389_residues
MREVAITGIGVVGPHGVGLAGVLDGRFVFSAWPDSASPPHAESLIASVGDYPRTRYFSERALRLMDKPMMLASFAAGAAL
EDAGYGDGEAPEDCATLLGTARSEQTSVHKFSLPLLQGRPERINPAEFPLVARNIACGQMAIRFGLRGPSSVLASGPLAS
LQAVARAAGLIRSGRAKVALAGGVEALSRFALRFCRDYYGDAALAGRPAFFGERGGGLIPSEGACMLVLEDAEHAAARGA
RVYARLGGWHAGRLGRGDAVQSLLDGWRRAADMAPALLLPGAGGAGRAHERLETLALRRWQAEGAAQPSRLLAARALAGE
GESWSGALQVALAAASLARRHAPATPDVAADADPALARAAAGGTLPDDAAALVLGNDAAGAFCALGLRR

Specific function: Catalyzes The Condensation Reaction Of Fatty Acid Synthesis By The Addition To An Acyl Acceptor Of Two Carbons From Malonyl-Acp. Has A Preference For Short Chain Acid Substrates And May Function To Supply The Octanoic Substrates For Lipoic Acid Biosynthe

COG id: COG0304

COG function: function code IQ; 3-oxoacyl-(acyl-carrier-protein) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-ketoacyl-ACP synthases family [H]

Homologues:

Organism=Homo sapiens, GI8923559, Length=266, Percent_Identity=29.3233082706767, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1787337, Length=263, Percent_Identity=29.277566539924, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71983541, Length=261, Percent_Identity=28.3524904214559, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24644341, Length=207, Percent_Identity=29.4685990338164, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000794
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C [H]

EC number: 2.3.1.41

Molecular weight: Translated: 40008; Mature: 40008

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREVAITGIGVVGPHGVGLAGVLDGRFVFSAWPDSASPPHAESLIASVGDYPRTRYFSER
CCCEEEEEEEEECCCCCCEEEEECCCEEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHH
ALRLMDKPMMLASFAAGAALEDAGYGDGEAPEDCATLLGTARSEQTSVHKFSLPLLQGRP
HHHHHCCCHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHEECHHHCCCC
ERINPAEFPLVARNIACGQMAIRFGLRGPSSVLASGPLASLQAVARAAGLIRSGRAKVAL
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEE
AGGVEALSRFALRFCRDYYGDAALAGRPAFFGERGGGLIPSEGACMLVLEDAEHAAARGA
CCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHCCH
RVYARLGGWHAGRLGRGDAVQSLLDGWRRAADMAPALLLPGAGGAGRAHERLETLALRRW
HEEHHHCCEECCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHH
QAEGAAQPSRLLAARALAGEGESWSGALQVALAAASLARRHAPATPDVAADADPALARAA
HHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
AGGTLPDDAAALVLGNDAAGAFCALGLRR
CCCCCCCCCEEEEECCCCCCHHHHHCCCC
>Mature Secondary Structure
MREVAITGIGVVGPHGVGLAGVLDGRFVFSAWPDSASPPHAESLIASVGDYPRTRYFSER
CCCEEEEEEEEECCCCCCEEEEECCCEEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHH
ALRLMDKPMMLASFAAGAALEDAGYGDGEAPEDCATLLGTARSEQTSVHKFSLPLLQGRP
HHHHHCCCHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHEECHHHCCCC
ERINPAEFPLVARNIACGQMAIRFGLRGPSSVLASGPLASLQAVARAAGLIRSGRAKVAL
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEE
AGGVEALSRFALRFCRDYYGDAALAGRPAFFGERGGGLIPSEGACMLVLEDAEHAAARGA
CCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHCCH
RVYARLGGWHAGRLGRGDAVQSLLDGWRRAADMAPALLLPGAGGAGRAHERLETLALRRW
HEEHHHCCEECCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHH
QAEGAAQPSRLLAARALAGEGESWSGALQVALAAASLARRHAPATPDVAADADPALARAA
HHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
AGGTLPDDAAALVLGNDAAGAFCALGLRR
CCCCCCCCCEEEEECCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: None [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} [C]

Substrates: acyl-[acyl-carrier protein]; malonyl-[acyl-carrier protein]

Specific reaction: acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl-ACP-decarboxylation, Cerulenin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1644304 [H]