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The map label for this gene is rph

Identifier: 34499302

GI number: 34499302

Start: 4152060

End: 4152776

Strand: Reverse

Name: rph

Synonym: CV_3847

Alternate gene names: 34499302

Gene position: 4152776-4152060 (Counterclockwise)

Preceding gene: 34499303

Following gene: 34499300

Centisome position: 87.41

GC content: 67.22

Gene sequence:

>717_bases
ATGCGACCTTCCCAACGTAGTGCCGACGCGATGCGCGTCGTGCGTCTGACCCGCTCTTACACCAAGCACGCCGAGGGTTC
GGTGCTGGTGGAGTTCGGCGACACCAAGGTGATCTGCACCGCCAGCGTCGAGGAAACGGTGCCGTCCTTTCTCAAGGGCA
AGGGACAGGGCTGGGTGACCGCCGAATACGGCATGCTGCCGCGCTCCACCGGCAGCCGCATGCGCCGCGAGTCGGCCGCC
GGCAAGCAGTCCGGCCGCACCCAGGAAATCCAGCGTCTGATCGGCCGCTCGCTGCGCGCGGTGACCGATCTGGCCAAGCT
GGGCGAGCGCCAGATCGTCATCGACTGCGACGTGATCCAGGCCGACGGCGGCACCCGTACCGCCAGCATCACCGGCGCCT
ATGTGGCGCTGGCCGACGCGATCCGCGGGCTGATCGATGCCGGCAAGCTGAGCGCCACCCCATTGCGCGACCAGGTCGCC
GCGGTGTCGGTGGGCGTGTACAAGGGTCAGCCGGTGCTGGATCTGGACTATCTGGAGGATTCCGACTGCGAGACCGATAT
GAACGTGGTGATGACCGGCAGCGGCCGCTTCGTCGAAGTGCAGGGCACCGCCGAAGGCGAGCCGTTCAGCGAGGAGGAGA
TGGCGGCGATGTTGGGGCTGGCGCGCAAGGGCATTGCCGAATTGCTGGCGCATCAGCGGCAGGCGCTGAACGTATGA

Upstream 100 bases:

>100_bases
ACCATGCACCAGGAAATCCTGGCCAGCCAGGTGGACAAGGACAGCTGAGCGCGGGCGGCTGTTATAATCGCCGTCGATCT
TCTTTATCCCCAGGGCCATC

Downstream 100 bases:

>100_bases
AGCCCAGGGTTTGAAGTCCGTTGCACAGAAAAAAGCCGCCTCGGGGCGGCTTTTTCATTTCTTGGCGGATTTGGCGATGA
AGGCCTGCAGCGCTTGCCGG

Product: tRNA nucleotidyltransferase

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRPSQRSADAMRVVRLTRSYTKHAEGSVLVEFGDTKVICTASVEETVPSFLKGKGQGWVTAEYGMLPRSTGSRMRRESAA
GKQSGRTQEIQRLIGRSLRAVTDLAKLGERQIVIDCDVIQADGGTRTASITGAYVALADAIRGLIDAGKLSATPLRDQVA
AVSVGVYKGQPVLDLDYLEDSDCETDMNVVMTGSGRFVEVQGTAEGEPFSEEEMAAMLGLARKGIAELLAHQRQALNV

Sequences:

>Translated_238_residues
MRPSQRSADAMRVVRLTRSYTKHAEGSVLVEFGDTKVICTASVEETVPSFLKGKGQGWVTAEYGMLPRSTGSRMRRESAA
GKQSGRTQEIQRLIGRSLRAVTDLAKLGERQIVIDCDVIQADGGTRTASITGAYVALADAIRGLIDAGKLSATPLRDQVA
AVSVGVYKGQPVLDLDYLEDSDCETDMNVVMTGSGRFVEVQGTAEGEPFSEEEMAAMLGLARKGIAELLAHQRQALNV
>Mature_238_residues
MRPSQRSADAMRVVRLTRSYTKHAEGSVLVEFGDTKVICTASVEETVPSFLKGKGQGWVTAEYGMLPRSTGSRMRRESAA
GKQSGRTQEIQRLIGRSLRAVTDLAKLGERQIVIDCDVIQADGGTRTASITGAYVALADAIRGLIDAGKLSATPLRDQVA
AVSVGVYKGQPVLDLDYLEDSDCETDMNVVMTGSGRFVEVQGTAEGEPFSEEEMAAMLGLARKGIAELLAHQRQALNV

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=69.1943127962085, Blast_Score=303, Evalue=5e-84,
Organism=Caenorhabditis elegans, GI71981632, Length=185, Percent_Identity=25.9459459459459, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_CHRVO (Q7MBD4)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903517.1
- ProteinModelPortal:   Q7MBD4
- SMR:   Q7MBD4
- GeneID:   2548708
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3847
- NMPDR:   fig|243365.1.peg.3847
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- PhylomeDB:   Q7MBD4
- BioCyc:   CVIO243365:CV_3847-MONOMER
- BRENDA:   2.7.7.56
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25565; Mature: 25565

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSQRSADAMRVVRLTRSYTKHAEGSVLVEFGDTKVICTASVEETVPSFLKGKGQGWVT
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCHHHHHHHHHCCCCCCEEE
AEYGMLPRSTGSRMRRESAAGKQSGRTQEIQRLIGRSLRAVTDLAKLGERQIVIDCDVIQ
EECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEE
ADGGTRTASITGAYVALADAIRGLIDAGKLSATPLRDQVAAVSVGVYKGQPVLDLDYLED
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEEEEECCCCEEEECCCCC
SDCETDMNVVMTGSGRFVEVQGTAEGEPFSEEEMAAMLGLARKGIAELLAHQRQALNV
CCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRPSQRSADAMRVVRLTRSYTKHAEGSVLVEFGDTKVICTASVEETVPSFLKGKGQGWVT
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCHHHHHHHHHCCCCCCEEE
AEYGMLPRSTGSRMRRESAAGKQSGRTQEIQRLIGRSLRAVTDLAKLGERQIVIDCDVIQ
EECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEE
ADGGTRTASITGAYVALADAIRGLIDAGKLSATPLRDQVAAVSVGVYKGQPVLDLDYLED
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEEEEECCCCEEEECCCCC
SDCETDMNVVMTGSGRFVEVQGTAEGEPFSEEEMAAMLGLARKGIAELLAHQRQALNV
CCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782