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The map label for this gene is fdoI [H]

Identifier: 34499296

GI number: 34499296

Start: 4146194

End: 4146811

Strand: Direct

Name: fdoI [H]

Synonym: CV_3841

Alternate gene names: 34499296

Gene position: 4146194-4146811 (Clockwise)

Preceding gene: 34499295

Following gene: 34499297

Centisome position: 87.27

GC content: 62.94

Gene sequence:

>618_bases
ATGAAGGAGAAACTGCTGCAGCGCTACAGCGCGGCGGAACGGATCAACCACTGGATCGTCGCCATCTGCTTCGTGCTGCT
GGCGCTGTCCGGGCTGGCGCTGTTCTACCCGGCCTTCTTCTGGCTGACCGGCGTGTTCGGCACGCCGCAGCTGGCGCGGA
TGGTGCATCCCTTCGTCGGCGTGCTGATGTTCCTGGGCTTCTTCCGCCAGTTTTTCCGCTACTGGCGGCGCAACCTGATC
AATAGCCAGGACATCCAGTGGATGCTGGCGGTGAAGGACGTGCTGCGCGGCCATGAGACCGTGGAAGTGGGCAAGTACAA
CGGCGGCCAGAAGGCGATGTTCTGGATCATGACCCTGTGCGTGGCCGTGATGCTGGTCACCGGCATCATCGCCTGGCGCC
AGTACTTCTCCGGCTATTTCCCCATCCCGGTGATCCGGCTGGCGCTGCTGCTGCACGCCTGGGCCGCCACCGCGCTGATC
GCCAGCATCATCGTCCACGTCTATGCCGCGCTGTGGGTGAAGGGCACGATACGGGCGATGGTGGAGGGCGTGGTCACCCA
CGCCTGGGCCAAGAAGCATCATCCGGGCTGGTATAAGGAAATGATGGGGAAGAAATAG

Upstream 100 bases:

>100_bases
TCGCCGCCGCCGGCCTGTTCGGCTTCCTGCACTACATCACCGTCGGGCCCAACCGCGCCGATGACGAAGCGGAAAGCGAA
ACCCGCGAGGAGGACAAGCA

Downstream 100 bases:

>100_bases
CGAGTGTCCGCTGCGCGGACGACATGTTTAGGTGCCGGTTCCGCCCGGCAGACGGGCCAGGGGCTGAGCGGCCAGCCCTA
TCGCTGAGTGAATCGAAGAT

Product: formate dehydrogenase

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MKEKLLQRYSAAERINHWIVAICFVLLALSGLALFYPAFFWLTGVFGTPQLARMVHPFVGVLMFLGFFRQFFRYWRRNLI
NSQDIQWMLAVKDVLRGHETVEVGKYNGGQKAMFWIMTLCVAVMLVTGIIAWRQYFSGYFPIPVIRLALLLHAWAATALI
ASIIVHVYAALWVKGTIRAMVEGVVTHAWAKKHHPGWYKEMMGKK

Sequences:

>Translated_205_residues
MKEKLLQRYSAAERINHWIVAICFVLLALSGLALFYPAFFWLTGVFGTPQLARMVHPFVGVLMFLGFFRQFFRYWRRNLI
NSQDIQWMLAVKDVLRGHETVEVGKYNGGQKAMFWIMTLCVAVMLVTGIIAWRQYFSGYFPIPVIRLALLLHAWAATALI
ASIIVHVYAALWVKGTIRAMVEGVVTHAWAKKHHPGWYKEMMGKK
>Mature_205_residues
MKEKLLQRYSAAERINHWIVAICFVLLALSGLALFYPAFFWLTGVFGTPQLARMVHPFVGVLMFLGFFRQFFRYWRRNLI
NSQDIQWMLAVKDVLRGHETVEVGKYNGGQKAMFWIMTLCVAVMLVTGIIAWRQYFSGYFPIPVIRLALLLHAWAATALI
ASIIVHVYAALWVKGTIRAMVEGVVTHAWAKKHHPGWYKEMMGKK

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=200, Percent_Identity=56.5, Blast_Score=243, Evalue=8e-66,
Organism=Escherichia coli, GI1787750, Length=202, Percent_Identity=48.5148514851485, Blast_Score=206, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 23651; Mature: 23651

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKLLQRYSAAERINHWIVAICFVLLALSGLALFYPAFFWLTGVFGTPQLARMVHPFVG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VLMFLGFFRQFFRYWRRNLINSQDIQWMLAVKDVLRGHETVEVGKYNGGQKAMFWIMTLC
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHH
VAVMLVTGIIAWRQYFSGYFPIPVIRLALLLHAWAATALIASIIVHVYAALWVKGTIRAM
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEGVVTHAWAKKHHPGWYKEMMGKK
HHHHHHHHHHHCCCCCHHHHHCCCC
>Mature Secondary Structure
MKEKLLQRYSAAERINHWIVAICFVLLALSGLALFYPAFFWLTGVFGTPQLARMVHPFVG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VLMFLGFFRQFFRYWRRNLINSQDIQWMLAVKDVLRGHETVEVGKYNGGQKAMFWIMTLC
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHH
VAVMLVTGIIAWRQYFSGYFPIPVIRLALLLHAWAATALIASIIVHVYAALWVKGTIRAM
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEGVVTHAWAKKHHPGWYKEMMGKK
HHHHHHHHHHHCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]