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The map label for this gene is fdoH [H]

Identifier: 34499295

GI number: 34499295

Start: 4145310

End: 4146197

Strand: Direct

Name: fdoH [H]

Synonym: CV_3840

Alternate gene names: 34499295

Gene position: 4145310-4146197 (Clockwise)

Preceding gene: 34499294

Following gene: 34499296

Centisome position: 87.25

GC content: 63.63

Gene sequence:

>888_bases
ATGCCATTGCAATCCCTGGATATCCACCGCCGCTCCGCCAGCCCGACCGAAAGCCCCAAGGTCCGCCAGACGCTGGAAGT
GGCCAAGCTGATCGACACCTCCACCTGCATAGGCTGCAAGGCCTGCCAGGTGGCTTGTTCCGAATGGAACGACATCCGCG
AGGAAGTCGGCCACAACATCGGGGTCTACGACAACCCCACCGACCTGTCCGCCAAGGCCTGGACGGTGATGCGCTTCGCC
GAGGAGGAAAGCAACGGCAAGCTGGAGTGGCTGATCCGCAAGGACGGCTGCATGCACTGCGCCGACCCCGGCTGTTTGAA
AGCCTGTCCGTCGCCGGGCGCCATCGTGCAGTACAGCAACGGCATCGTCGACTTCCACCAGGAAAACTGCATCGGCTGCG
GTTACTGCATCGCCGGCTGTCCGTTCAATATTCCGCGCATCAGCAAGGAAGACAACAAGGCTTACAAGTGCACGCTGTGC
TCGGACCGCGTCGCCGTCGGCCTGGAGCCGGCTTGCGTCAAGACCTGTCCCACCGGGGCCATCCAGTTCGGCTCCAAGGA
AGACATGAAGGAAGTGGCGGCGGAACGGGTGGCGGATCTGAAGCGGCGCGGCTATCAACACGCCGGCCTGTACGATCCGC
AAGGCGTGGGCGGCACCCACGTGATGTACGTGCTGCACCACGCCGACAAGCCGGAACAGTACTCCGGCCTGCCGAACAAC
CCCGAAATCAGCCCCACCGTGCAGCTGTGGAAGGGCTTGCTCAAGCCGCTGGCGACGATAGGCATCGCCGCCGCCGGCCT
GTTCGGCTTCCTGCACTACATCACCGTCGGGCCCAACCGCGCCGATGACGAAGCGGAAAGCGAAACCCGCGAGGAGGACA
AGCAATGA

Upstream 100 bases:

>100_bases
GACCAATGCGCTGCCGCCCGCGGTCGGCGACTGCAACACCCAGACACCCGAGTACAAGGCCTTCCTGGTCAAGCTGGAAA
AACTGTAAGGAGCCCCGGCC

Downstream 100 bases:

>100_bases
AGGAGAAACTGCTGCAGCGCTACAGCGCGGCGGAACGGATCAACCACTGGATCGTCGCCATCTGCTTCGTGCTGCTGGCG
CTGTCCGGGCTGGCGCTGTT

Product: formate dehydrogenase

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase iron-sulfur subunit; FDH-Z subunit beta; Formate dehydrogenase-O subunit beta [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MPLQSLDIHRRSASPTESPKVRQTLEVAKLIDTSTCIGCKACQVACSEWNDIREEVGHNIGVYDNPTDLSAKAWTVMRFA
EEESNGKLEWLIRKDGCMHCADPGCLKACPSPGAIVQYSNGIVDFHQENCIGCGYCIAGCPFNIPRISKEDNKAYKCTLC
SDRVAVGLEPACVKTCPTGAIQFGSKEDMKEVAAERVADLKRRGYQHAGLYDPQGVGGTHVMYVLHHADKPEQYSGLPNN
PEISPTVQLWKGLLKPLATIGIAAAGLFGFLHYITVGPNRADDEAESETREEDKQ

Sequences:

>Translated_295_residues
MPLQSLDIHRRSASPTESPKVRQTLEVAKLIDTSTCIGCKACQVACSEWNDIREEVGHNIGVYDNPTDLSAKAWTVMRFA
EEESNGKLEWLIRKDGCMHCADPGCLKACPSPGAIVQYSNGIVDFHQENCIGCGYCIAGCPFNIPRISKEDNKAYKCTLC
SDRVAVGLEPACVKTCPTGAIQFGSKEDMKEVAAERVADLKRRGYQHAGLYDPQGVGGTHVMYVLHHADKPEQYSGLPNN
PEISPTVQLWKGLLKPLATIGIAAAGLFGFLHYITVGPNRADDEAESETREEDKQ
>Mature_294_residues
PLQSLDIHRRSASPTESPKVRQTLEVAKLIDTSTCIGCKACQVACSEWNDIREEVGHNIGVYDNPTDLSAKAWTVMRFAE
EESNGKLEWLIRKDGCMHCADPGCLKACPSPGAIVQYSNGIVDFHQENCIGCGYCIAGCPFNIPRISKEDNKAYKCTLCS
DRVAVGLEPACVKTCPTGAIQFGSKEDMKEVAAERVADLKRRGYQHAGLYDPQGVGGTHVMYVLHHADKPEQYSGLPNNP
EISPTVQLWKGLLKPLATIGIAAAGLFGFLHYITVGPNRADDEAESETREEDKQ

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790326, Length=298, Percent_Identity=71.1409395973154, Blast_Score=448, Evalue=1e-127,
Organism=Escherichia coli, GI1787749, Length=294, Percent_Identity=70.0680272108844, Blast_Score=439, Evalue=1e-125,
Organism=Escherichia coli, GI1789370, Length=274, Percent_Identity=27.007299270073, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787872, Length=172, Percent_Identity=33.7209302325581, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787122, Length=180, Percent_Identity=32.2222222222222, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI2367345, Length=158, Percent_Identity=32.9113924050633, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI226510944, Length=177, Percent_Identity=31.638418079096, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1788811, Length=188, Percent_Identity=29.2553191489362, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787963, Length=154, Percent_Identity=24.025974025974, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082179, Length=173, Percent_Identity=26.0115606936416, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787478, Length=193, Percent_Identity=27.4611398963731, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787740, Length=112, Percent_Identity=30.3571428571429, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI87082180, Length=166, Percent_Identity=27.710843373494, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 32257; Mature: 32126

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
5.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLQSLDIHRRSASPTESPKVRQTLEVAKLIDTSTCIGCKACQVACSEWNDIREEVGHNI
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHCCCC
GVYDNPTDLSAKAWTVMRFAEEESNGKLEWLIRKDGCMHCADPGCLKACPSPGAIVQYSN
CCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHCCCCCCCEEEECC
GIVDFHQENCIGCGYCIAGCPFNIPRISKEDNKAYKCTLCSDRVAVGLEPACVKTCPTGA
CEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCHHHHHHCCCCC
IQFGSKEDMKEVAAERVADLKRRGYQHAGLYDPQGVGGTHVMYVLHHADKPEQYSGLPNN
EECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHCCCCCC
PEISPTVQLWKGLLKPLATIGIAAAGLFGFLHYITVGPNRADDEAESETREEDKQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
PLQSLDIHRRSASPTESPKVRQTLEVAKLIDTSTCIGCKACQVACSEWNDIREEVGHNI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHCCCC
GVYDNPTDLSAKAWTVMRFAEEESNGKLEWLIRKDGCMHCADPGCLKACPSPGAIVQYSN
CCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHCCCCCCCEEEECC
GIVDFHQENCIGCGYCIAGCPFNIPRISKEDNKAYKCTLCSDRVAVGLEPACVKTCPTGA
CEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCHHHHHHCCCCC
IQFGSKEDMKEVAAERVADLKRRGYQHAGLYDPQGVGGTHVMYVLHHADKPEQYSGLPNN
EECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHCCCCCC
PEISPTVQLWKGLLKPLATIGIAAAGLFGFLHYITVGPNRADDEAESETREEDKQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]