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The map label for this gene is greA [H]

Identifier: 34499256

GI number: 34499256

Start: 4097810

End: 4098286

Strand: Reverse

Name: greA [H]

Synonym: CV_3801

Alternate gene names: 34499256

Gene position: 4098286-4097810 (Counterclockwise)

Preceding gene: 34499257

Following gene: 34499255

Centisome position: 86.26

GC content: 62.89

Gene sequence:

>477_bases
ATGATCAAAGTCCCGTTGACTGTACGCGGCGCCGAGCTTCTGAAGGAAGAACTGCAACGCCTGAAGAGCGTGGAGCGTCC
GTCGGTGATCGAGGCGATCGCCGAAGCCCGCTCCCATGGCGACCTGTCGGAAAATGCCGAGTACGACGCGGCCAAGGAAC
GCCAAGCCTTCGTCGAAGGCCGCATCGCCGAGCTGGAAGGCAAGCTGTCCAACGCCGTGATCATCAATCCGGCCGAGCTT
GACGCCGACGGCCGCGTGGTGTTCGGCGCCACCGTCGACCTGATGGACCTGGAAACCGAAGAGAGCGTCACCTACCAGAT
CGTCGGCGACGACGAGGCCGACATCAAGGTGTGCAAGGTATCGGTCAACTCGCCGATCTCGCGCGCGCTGATCGGCAAGG
AAGCCGGCGACGTGGCCGAAGTGGTGGCCCCTGGCGGCATCCGCGAGTACGAAGTGCTCGACGTCAAATACATCTGA

Upstream 100 bases:

>100_bases
CCGTTACAATTCCGCTCAAACCCGCTCGCGCAAGCCGCCGTCCCGTACGGCGGCTTGTGCTTTTGATGAAGGCGGACAAC
CGCCTGTGGAGAATACTGTA

Downstream 100 bases:

>100_bases
TGCGGACCGCGCGTTTTTCATGAGCTGCGTCTGTTGCAGGGGGAGAGATGGACGGCTTGCGTGCAGTGGCCAAGACCTTG
TGGATTGGCGGTTTGTGGGT

Product: transcription elongation factor

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MIKVPLTVRGAELLKEELQRLKSVERPSVIEAIAEARSHGDLSENAEYDAAKERQAFVEGRIAELEGKLSNAVIINPAEL
DADGRVVFGATVDLMDLETEESVTYQIVGDDEADIKVCKVSVNSPISRALIGKEAGDVAEVVAPGGIREYEVLDVKYI

Sequences:

>Translated_158_residues
MIKVPLTVRGAELLKEELQRLKSVERPSVIEAIAEARSHGDLSENAEYDAAKERQAFVEGRIAELEGKLSNAVIINPAEL
DADGRVVFGATVDLMDLETEESVTYQIVGDDEADIKVCKVSVNSPISRALIGKEAGDVAEVVAPGGIREYEVLDVKYI
>Mature_158_residues
MIKVPLTVRGAELLKEELQRLKSVERPSVIEAIAEARSHGDLSENAEYDAAKERQAFVEGRIAELEGKLSNAVIINPAEL
DADGRVVFGATVDLMDLETEESVTYQIVGDDEADIKVCKVSVNSPISRALIGKEAGDVAEVVAPGGIREYEVLDVKYI

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=58.2278481012658, Blast_Score=187, Evalue=3e-49,
Organism=Escherichia coli, GI87082261, Length=160, Percent_Identity=38.125, Blast_Score=92, Evalue=1e-20,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17139; Mature: 17139

Theoretical pI: Translated: 4.21; Mature: 4.21

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVPLTVRGAELLKEELQRLKSVERPSVIEAIAEARSHGDLSENAEYDAAKERQAFVEG
CEECCEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHC
RIAELEGKLSNAVIINPAELDADGRVVFGATVDLMDLETEESVTYQIVGDDEADIKVCKV
HHHHHCCCCCCEEEEECHHCCCCCCEEEEEEEEEEECCCCCCEEEEEECCCCCCEEEEEE
SVNSPISRALIGKEAGDVAEVVAPGGIREYEVLDVKYI
ECCCCHHHHHHCCCCCCHHHHHCCCCCCEEEEEEEEEC
>Mature Secondary Structure
MIKVPLTVRGAELLKEELQRLKSVERPSVIEAIAEARSHGDLSENAEYDAAKERQAFVEG
CEECCEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHC
RIAELEGKLSNAVIINPAELDADGRVVFGATVDLMDLETEESVTYQIVGDDEADIKVCKV
HHHHHCCCCCCEEEEECHHCCCCCCEEEEEEEEEEECCCCCCEEEEEECCCCCCEEEEEE
SVNSPISRALIGKEAGDVAEVVAPGGIREYEVLDVKYI
ECCCCHHHHHHCCCCCCHHHHHCCCCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]