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The map label for this gene is queF

Identifier: 34499205

GI number: 34499205

Start: 4048204

End: 4049043

Strand: Direct

Name: queF

Synonym: CV_3750

Alternate gene names: 34499205

Gene position: 4048204-4049043 (Clockwise)

Preceding gene: 34499204

Following gene: 34499208

Centisome position: 85.21

GC content: 66.55

Gene sequence:

>840_bases
ATGAACATACATGCCGCCACACCCGAACATTCGCCGCTGGGCAAAACCGTCAGCTACCAGGACCAGTACGATCCGTCCCT
GCTGTTCCCGATCGCGCGCCAGACCAAGCGCGACGAGATCGGCGTGGATGAAGCCGCGCTGCCCTTCGCCGGCGTCGACA
TCTGGACCGGCTTCGAGCTGTCCTGGCTCAACGCCCGCGGCAAGCCGCAGATCGGCATCGCCACCTTCCGCATTCCGGCC
GGCAGCCCCAGGCTGATCGAGTCCAAGTCGTTCAAGCTCTACCTGAACAGCTACAACCAGACGCGGATGGACGGCATCGA
CGCCCTCGCCGCCCAGTTGGCGCGGGACCTGTCCGCCGCCGCCGGCGCCGAGGTGGCGGTCAGCATCGCGCTGCCGCAAG
CCTTCGCCGCCGAGCGGATCGCCGAGCTCGCGGGCGAATGCATCGACGAGCTGGACATCGCCGTCGACAACTACGCGCCC
TGCCCGGAAATCCTGAGCGCCGACTCCACGGCCATCGTCAGCGAAACGCTGTGCAGCAATCTCTTGAAGTCCAACTGCCT
GGTCACCGGCCAGCCGGACTGGGGCAGCGTGTCCATCCGCTACACCGGTCCGAAAATAGACCGCGAAGCGCTGCTGAGGT
ATCTGATCGGCTTCCGCCGGCACAATGAATTCCACGAGCAATGCGTCGAGCGCATCTTCGTCGACGTGCTGCGCGCCTGC
GCGCCAACGAAGCTGACCGTCTACGCCCGCTACACCCGCCGCGGCGGCCTGGACATCAACCCCTGGCGCAGCAACTGCGA
CGCAGCGCCGACGGACAACGTCCGCACCGCCCGGCAGTAG

Upstream 100 bases:

>100_bases
ATAAATCAATCTGGCTGGGCGTGGCGCTGCATTGCAGCGTGGCGCTGACCATGGACCTGATGGCGCTGTGGCGCAAGGGT
CTGCTGTAAAGGAAATCATC

Downstream 100 bases:

>100_bases
CGCCGGCGAAACGCGCAAAACAAAAGGGATGCTTCTCGGCATCCCTTTTTTTGCGGCCCAGGCCAGGCATCAGAACGGCG
CCGGCGCCTCGAACTCCATT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MNIHAATPEHSPLGKTVSYQDQYDPSLLFPIARQTKRDEIGVDEAALPFAGVDIWTGFELSWLNARGKPQIGIATFRIPA
GSPRLIESKSFKLYLNSYNQTRMDGIDALAAQLARDLSAAAGAEVAVSIALPQAFAAERIAELAGECIDELDIAVDNYAP
CPEILSADSTAIVSETLCSNLLKSNCLVTGQPDWGSVSIRYTGPKIDREALLRYLIGFRRHNEFHEQCVERIFVDVLRAC
APTKLTVYARYTRRGGLDINPWRSNCDAAPTDNVRTARQ

Sequences:

>Translated_279_residues
MNIHAATPEHSPLGKTVSYQDQYDPSLLFPIARQTKRDEIGVDEAALPFAGVDIWTGFELSWLNARGKPQIGIATFRIPA
GSPRLIESKSFKLYLNSYNQTRMDGIDALAAQLARDLSAAAGAEVAVSIALPQAFAAERIAELAGECIDELDIAVDNYAP
CPEILSADSTAIVSETLCSNLLKSNCLVTGQPDWGSVSIRYTGPKIDREALLRYLIGFRRHNEFHEQCVERIFVDVLRAC
APTKLTVYARYTRRGGLDINPWRSNCDAAPTDNVRTARQ
>Mature_279_residues
MNIHAATPEHSPLGKTVSYQDQYDPSLLFPIARQTKRDEIGVDEAALPFAGVDIWTGFELSWLNARGKPQIGIATFRIPA
GSPRLIESKSFKLYLNSYNQTRMDGIDALAAQLARDLSAAAGAEVAVSIALPQAFAAERIAELAGECIDELDIAVDNYAP
CPEILSADSTAIVSETLCSNLLKSNCLVTGQPDWGSVSIRYTGPKIDREALLRYLIGFRRHNEFHEQCVERIFVDVLRAC
APTKLTVYARYTRRGGLDINPWRSNCDAAPTDNVRTARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=269, Percent_Identity=54.275092936803, Blast_Score=303, Evalue=8e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_CHRVO (Q7NRN0)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903420.1
- ProteinModelPortal:   Q7NRN0
- SMR:   Q7NRN0
- GeneID:   2549178
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3750
- NMPDR:   fig|243365.1.peg.3750
- HOGENOM:   HBG289139
- OMA:   PFRSNFE
- PhylomeDB:   Q7NRN0
- ProtClustDB:   PRK11792
- BioCyc:   CVIO243365:CV_3750-MONOMER
- BRENDA:   1.7.1.13
- GO:   GO:0005737
- HAMAP:   MF_00817
- InterPro:   IPR016428
- InterPro:   IPR020602
- PIRSF:   PIRSF004750
- TIGRFAMs:   TIGR03138

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 30700; Mature: 30700

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIHAATPEHSPLGKTVSYQDQYDPSLLFPIARQTKRDEIGVDEAALPFAGVDIWTGFEL
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHCCCCCCCCCCCCCCCCCCEEECCCEE
SWLNARGKPQIGIATFRIPAGSPRLIESKSFKLYLNSYNQTRMDGIDALAAQLARDLSAA
EEECCCCCCCEEEEEEEECCCCCCEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
AGAEVAVSIALPQAFAAERIAELAGECIDELDIAVDNYAPCPEILSADSTAIVSETLCSN
CCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHH
LLKSNCLVTGQPDWGSVSIRYTGPKIDREALLRYLIGFRRHNEFHEQCVERIFVDVLRAC
HHHCCCEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHC
APTKLTVYARYTRRGGLDINPWRSNCDAAPTDNVRTARQ
CCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNIHAATPEHSPLGKTVSYQDQYDPSLLFPIARQTKRDEIGVDEAALPFAGVDIWTGFEL
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHCCCCCCCCCCCCCCCCCCEEECCCEE
SWLNARGKPQIGIATFRIPAGSPRLIESKSFKLYLNSYNQTRMDGIDALAAQLARDLSAA
EEECCCCCCCEEEEEEEECCCCCCEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
AGAEVAVSIALPQAFAAERIAELAGECIDELDIAVDNYAPCPEILSADSTAIVSETLCSN
CCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHH
LLKSNCLVTGQPDWGSVSIRYTGPKIDREALLRYLIGFRRHNEFHEQCVERIFVDVLRAC
HHHCCCEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHC
APTKLTVYARYTRRGGLDINPWRSNCDAAPTDNVRTARQ
CCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782