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The map label for this gene is ackA [H]

Identifier: 34499200

GI number: 34499200

Start: 4043474

End: 4044625

Strand: Direct

Name: ackA [H]

Synonym: CV_3745

Alternate gene names: 34499200

Gene position: 4043474-4044625 (Clockwise)

Preceding gene: 34499199

Following gene: 34499203

Centisome position: 85.11

GC content: 71.01

Gene sequence:

>1152_bases
ATGAACCGCTGCCTGCTGGCGATCAATGCCGGTTCCGCCACGCTGAAGTTCCGCGCCTTCTCCCCGGATGGCCGGACGCT
GCTGGCGCGCGGCATGGTGGACCGTTTCGGCAGCAAGGAGGCCAGGCTGCGCTTGGCCGACGCCAAGGGCCAGGCCCTGG
CCGAGCGCGATCTGGCCGACGCAGCCCACGCCGCCGCCCTGGCCGCGGTACTCAACAGCCTGGCCGACCAGGGTCTGGAG
GCGCAGGCCCTGGTCCACCGCGTGGTGCACGGCGGCAGCCGCTTCCGCGAGCCGGTGCGGATAGACGCCGAAGTCCGCGC
GGCGCTGGACGACTACATCGCGCTGGCGCCGCTGCACCAGCCGGTGAGCCTGGAGGTGGTGGACGCGTTCAGCGAACTGG
ATCCGCGGCTGCCGCAGATTGCCTGCTTCGACACCGCCTTCCACGCCACCCAGCCCACGGTGGCCACCCGTTTCGGCATT
GCCCGCCACTGGCACGACGAGGGCGTGCGCCGCTATGGCTTCCACGGCCTGTCCTACGCCGCCATCTCGCGGCGGCTGCC
GGAGCTGGGGCTTGCCCACGGCAAGGTGGTGGTCTGCCACCTGGGCAGCGGCGCCAGCGCCTGCGCGATCGAGTCCGGCA
AGAGCGTGGCGTCCAGCATGGGCTTCTCGGCGGTGGACGGGCTGATGATGGGCAGCCGTCCAGGCTATATCGACCCGGAG
GTGATCCTGTACTGGCAGGAACACGAAGGCATGGGCGTGAAGGACGTGCGGCGCGAGCTGTACAAGAACTCGGGCCTCTT
GGGGGTGTCCGGCGTATCCGCCGACATGCGCGAACTGCTGGCCAGCGACTTGCCGGAAGCGCGCGAGGCGGTGGAGCTGT
TCTGCTACCGCGCCGCGCGCGAGGCGGCCAGCCTGGCCGCGGCGATGCGCGGCCTGGACGGGCTGGTCTTCACCGCCGGC
ATAGGCGAACACTCGGCCGAGGTGCGGGCCAGGATATTGCAGCAGCTGTCCTGGCTGGGATTCGATCTCGACCACGCCGC
CAACCTCGCCCACGCGCGGCGGCTGACCGCCGCCGGCAGCCGCCTGCCGGCCTACGTGCTGCCCACCGACGAGGAGGGCG
AAATGGCGCGCCAGGCCGCGCGCTTGTTATAA

Upstream 100 bases:

>100_bases
TGGTGCTGACCAGCCGCGCCGACGACGCCAGCGGCCGCCTCGCCTCCAGCGCCATCGCCAATCTGCTGGCCCACCAGCGC
CGCCAACAGGGAGGGCGTCC

Downstream 100 bases:

>100_bases
AAACAGGGTCAGGCGCCCGCGCCGCCCAGCCAGCGGGTGAGCACCTGAGCGGCCAGCGCCGTCAGCGCCTCGCCATGGCG
TCGGTTCTGCTCGCGCAGCT

Product: acetate kinase family protein

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MNRCLLAINAGSATLKFRAFSPDGRTLLARGMVDRFGSKEARLRLADAKGQALAERDLADAAHAAALAAVLNSLADQGLE
AQALVHRVVHGGSRFREPVRIDAEVRAALDDYIALAPLHQPVSLEVVDAFSELDPRLPQIACFDTAFHATQPTVATRFGI
ARHWHDEGVRRYGFHGLSYAAISRRLPELGLAHGKVVVCHLGSGASACAIESGKSVASSMGFSAVDGLMMGSRPGYIDPE
VILYWQEHEGMGVKDVRRELYKNSGLLGVSGVSADMRELLASDLPEAREAVELFCYRAAREAASLAAAMRGLDGLVFTAG
IGEHSAEVRARILQQLSWLGFDLDHAANLAHARRLTAAGSRLPAYVLPTDEEGEMARQAARLL

Sequences:

>Translated_383_residues
MNRCLLAINAGSATLKFRAFSPDGRTLLARGMVDRFGSKEARLRLADAKGQALAERDLADAAHAAALAAVLNSLADQGLE
AQALVHRVVHGGSRFREPVRIDAEVRAALDDYIALAPLHQPVSLEVVDAFSELDPRLPQIACFDTAFHATQPTVATRFGI
ARHWHDEGVRRYGFHGLSYAAISRRLPELGLAHGKVVVCHLGSGASACAIESGKSVASSMGFSAVDGLMMGSRPGYIDPE
VILYWQEHEGMGVKDVRRELYKNSGLLGVSGVSADMRELLASDLPEAREAVELFCYRAAREAASLAAAMRGLDGLVFTAG
IGEHSAEVRARILQQLSWLGFDLDHAANLAHARRLTAAGSRLPAYVLPTDEEGEMARQAARLL
>Mature_383_residues
MNRCLLAINAGSATLKFRAFSPDGRTLLARGMVDRFGSKEARLRLADAKGQALAERDLADAAHAAALAAVLNSLADQGLE
AQALVHRVVHGGSRFREPVRIDAEVRAALDDYIALAPLHQPVSLEVVDAFSELDPRLPQIACFDTAFHATQPTVATRFGI
ARHWHDEGVRRYGFHGLSYAAISRRLPELGLAHGKVVVCHLGSGASACAIESGKSVASSMGFSAVDGLMMGSRPGYIDPE
VILYWQEHEGMGVKDVRRELYKNSGLLGVSGVSADMRELLASDLPEAREAVELFCYRAAREAASLAAAMRGLDGLVFTAG
IGEHSAEVRARILQQLSWLGFDLDHAANLAHARRLTAAGSRLPAYVLPTDEEGEMARQAARLL

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=402, Percent_Identity=36.81592039801, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI145693184, Length=392, Percent_Identity=35.969387755102, Blast_Score=221, Evalue=5e-59,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 41180; Mature: 41180

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRCLLAINAGSATLKFRAFSPDGRTLLARGMVDRFGSKEARLRLADAKGQALAERDLAD
CCCEEEEEECCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHH
AAHAAALAAVLNSLADQGLEAQALVHRVVHGGSRFREPVRIDAEVRAALDDYIALAPLHQ
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHCCCCEECHHHHHHHHHHHHHCCCCC
PVSLEVVDAFSELDPRLPQIACFDTAFHATQPTVATRFGIARHWHDEGVRRYGFHGLSYA
CCCHHHHHHHHHCCCCCCCEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AISRRLPELGLAHGKVVVCHLGSGASACAIESGKSVASSMGFSAVDGLMMGSRPGYIDPE
HHHHCCHHHCCCCCCEEEEECCCCCCEEEECCCHHHHHHCCHHHHHHHHCCCCCCCCCCE
VILYWQEHEGMGVKDVRRELYKNSGLLGVSGVSADMRELLASDLPEAREAVELFCYRAAR
EEEEEECCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
EAASLAAAMRGLDGLVFTAGIGEHSAEVRARILQQLSWLGFDLDHAANLAHARRLTAAGS
HHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
RLPAYVLPTDEEGEMARQAARLL
CCCEEEECCCCCHHHHHHHHHCC
>Mature Secondary Structure
MNRCLLAINAGSATLKFRAFSPDGRTLLARGMVDRFGSKEARLRLADAKGQALAERDLAD
CCCEEEEEECCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHH
AAHAAALAAVLNSLADQGLEAQALVHRVVHGGSRFREPVRIDAEVRAALDDYIALAPLHQ
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHCCCCEECHHHHHHHHHHHHHCCCCC
PVSLEVVDAFSELDPRLPQIACFDTAFHATQPTVATRFGIARHWHDEGVRRYGFHGLSYA
CCCHHHHHHHHHCCCCCCCEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AISRRLPELGLAHGKVVVCHLGSGASACAIESGKSVASSMGFSAVDGLMMGSRPGYIDPE
HHHHCCHHHCCCCCCEEEEECCCCCCEEEECCCHHHHHHCCHHHHHHHHCCCCCCCCCCE
VILYWQEHEGMGVKDVRRELYKNSGLLGVSGVSADMRELLASDLPEAREAVELFCYRAAR
EEEEEECCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
EAASLAAAMRGLDGLVFTAGIGEHSAEVRARILQQLSWLGFDLDHAANLAHARRLTAAGS
HHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
RLPAYVLPTDEEGEMARQAARLL
CCCEEEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA