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The map label for this gene is trmD [H]

Identifier: 34499128

GI number: 34499128

Start: 3973927

End: 3974613

Strand: Reverse

Name: trmD [H]

Synonym: CV_3673

Alternate gene names: 34499128

Gene position: 3974613-3973927 (Counterclockwise)

Preceding gene: 34499129

Following gene: 34499127

Centisome position: 83.66

GC content: 65.07

Gene sequence:

>687_bases
TTGGGCCTCTGGCAGTTCAAGGCCTGGAACCCGCGCGACTTCACCCACGACAATTATCGTCGGGTGGATGACCGGCCCTA
TGGCGGCGGCCCCGGCATGGTGATGCAGATCGAGCCGCTGGAGCAGGCGCTGGATGCCGCGCGCGCGCGCCAGCGCGAAG
CCGGCGTCGAGGCCAGCCACGTGGTCTACCTGTCGCCGCAAGGCGCGCGGCTGACCCACGCCAAGGCGGCGGAGCTGTCG
CAGCGGCCAGGTTTGATCCTGCTGTGCGGCCGCTACGAAGGCATAGACGAGCGCTTGATCGCCACGCAGGTCGACGAGGA
GATTTCCATCGGCGACTACGTGCTGTCGGGCGGCGAATTGCCGGCGATGGTGCTGGCCGACGCGGTGGTCCGGCTGCTGC
CGGGCGCGCTGAACGACGCCCAGTCGGCGTACGAAGACTCATTTGTGGACGGTCTCTTGGACTGCCCGCACTATACCCGA
CCCGAAGAGTATCGAGGCATGCGGGTGCCGGACGTGCTGTTGTCCGGTAATCATGCCCTGATCGCCAAATGGCGGCTCAA
GCAGTCGCTGGGCAGGACGTGGCAACGTCGGCCCGAGCTGCTGCAAGACCGCGTTTTGACAAAACAGGAATCTCGGCTGC
TGGCGGAGTACCAGCAGGAACAAGATATCCGCAAAAAACCGGAGTAA

Upstream 100 bases:

>100_bases
ACTGGGGTCTGGATTACTGATGCAGATCGACGCGGTGACGCTGTTCCCGGAGATGTTCGATTCGATTGCCCGCTTCGGCG
TCAGCCAGCGGGCGCTTGAC

Downstream 100 bases:

>100_bases
AACATGGATCTGATCCAGAAACTCGAGCAAGAAGAAATCGCACGCCTGGGCAAGACCCTGCCGGAATTCGCCCCGGGCGA
TACCGTCGTGGTTCAAGTCA

Product: tRNA (guanine-N1-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MGLWQFKAWNPRDFTHDNYRRVDDRPYGGGPGMVMQIEPLEQALDAARARQREAGVEASHVVYLSPQGARLTHAKAAELS
QRPGLILLCGRYEGIDERLIATQVDEEISIGDYVLSGGELPAMVLADAVVRLLPGALNDAQSAYEDSFVDGLLDCPHYTR
PEEYRGMRVPDVLLSGNHALIAKWRLKQSLGRTWQRRPELLQDRVLTKQESRLLAEYQQEQDIRKKPE

Sequences:

>Translated_228_residues
MGLWQFKAWNPRDFTHDNYRRVDDRPYGGGPGMVMQIEPLEQALDAARARQREAGVEASHVVYLSPQGARLTHAKAAELS
QRPGLILLCGRYEGIDERLIATQVDEEISIGDYVLSGGELPAMVLADAVVRLLPGALNDAQSAYEDSFVDGLLDCPHYTR
PEEYRGMRVPDVLLSGNHALIAKWRLKQSLGRTWQRRPELLQDRVLTKQESRLLAEYQQEQDIRKKPE
>Mature_227_residues
GLWQFKAWNPRDFTHDNYRRVDDRPYGGGPGMVMQIEPLEQALDAARARQREAGVEASHVVYLSPQGARLTHAKAAELSQ
RPGLILLCGRYEGIDERLIATQVDEEISIGDYVLSGGELPAMVLADAVVRLLPGALNDAQSAYEDSFVDGLLDCPHYTRP
EEYRGMRVPDVLLSGNHALIAKWRLKQSLGRTWQRRPELLQDRVLTKQESRLLAEYQQEQDIRKKPE

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=220, Percent_Identity=62.7272727272727, Blast_Score=275, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 25820; Mature: 25688

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLWQFKAWNPRDFTHDNYRRVDDRPYGGGPGMVMQIEPLEQALDAARARQREAGVEASH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCE
VVYLSPQGARLTHAKAAELSQRPGLILLCGRYEGIDERLIATQVDEEISIGDYVLSGGEL
EEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCEEECCCCC
PAMVLADAVVRLLPGALNDAQSAYEDSFVDGLLDCPHYTRPEEYRGMRVPDVLLSGNHAL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEECCCCEE
IAKWRLKQSLGRTWQRRPELLQDRVLTKQESRLLAEYQQEQDIRKKPE
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GLWQFKAWNPRDFTHDNYRRVDDRPYGGGPGMVMQIEPLEQALDAARARQREAGVEASH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCE
VVYLSPQGARLTHAKAAELSQRPGLILLCGRYEGIDERLIATQVDEEISIGDYVLSGGEL
EEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCEEECCCCC
PAMVLADAVVRLLPGALNDAQSAYEDSFVDGLLDCPHYTRPEEYRGMRVPDVLLSGNHAL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEECCCCEE
IAKWRLKQSLGRTWQRRPELLQDRVLTKQESRLLAEYQQEQDIRKKPE
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]