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The map label for this gene is trmJ [H]

Identifier: 34499100

GI number: 34499100

Start: 3942523

End: 3943257

Strand: Direct

Name: trmJ [H]

Synonym: CV_3645

Alternate gene names: 34499100

Gene position: 3942523-3943257 (Clockwise)

Preceding gene: 34499089

Following gene: 34499101

Centisome position: 82.98

GC content: 66.94

Gene sequence:

>735_bases
CTGCAAAACATCCGCGTGGTGCTGGCCCGCCCCAACCATCCGGGCAATATCGGCTCGGCCGCGCGCGCGATGAAAACCAT
GGGTTTGAGCCGCCTCTACCTGGTCGAACCCAAGGTGTTTCCCAGCGAAGAGGCCAACGCGCTGGCCTCCGGCGCGGTGG
ACCTGCTGGAGAACGCCACCGTGGTCGGCTCGCTGCGGGAGGCGTTGTCCGACGTCACCGTCGCCTGCGCGCTGACCAGC
CGCCGCCGCGAACTGACCACCCCGCTGTCCACTCCGCGCCAGACCACGCCGGAGCTGATCGCCCGCGCCCGCGACGGCGA
ACAGGTGGCCCTGGTGTTCGGCAACGAAACCTTCGGCCTGTCGATCGAGGAGGTGGAGCAATGCAACCGTCTGGTGACCA
TCCCCGGCAACCCCGATTACTTCTCGCTTAACCTGGCGATGGCGGTCCAGGTGATGACCTACGAGCTATTCAGCCACACC
GGCGTGGACGTGGAATACCTGCGCGCCGAGGGCGAGGCGGCGACGCAAAGCGAGGTGGACGGCATGTGCGGCCACCTGGA
GCAGGCGATGGACGAGATCGGCTACTTCCAGCGCCGCAACAGCGAGCGGCTGATGCGCCGGATGCGCACCATGTTCCACC
GCGCCGGCATGCTGCGCGAGGAGATCGACATCCTGCGCGGTTTCTACAAACAGGTGCAGCGCACCGCTTCTGGCCGCTCC
GGAGATGCCGACTGA

Upstream 100 bases:

>100_bases
TAAGGAACAAAAAAACCCGACGCCTGGGCGCGGGCTTTTGGTATATGCCCGCTATTTTAATGTGAAAACGCCAATGAATA
AACCCCAAGTACCTGATTTT

Downstream 100 bases:

>100_bases
GCCCGGCCTCACCCTGCCCTACGCGCACCCCGCGGTGCGCGACCTCGCCTTCCTGCTGACCTCCCCCTCCCCCTGGCGCT
GCGGCGCCGATCTGCCGCCG

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MQNIRVVLARPNHPGNIGSAARAMKTMGLSRLYLVEPKVFPSEEANALASGAVDLLENATVVGSLREALSDVTVACALTS
RRRELTTPLSTPRQTTPELIARARDGEQVALVFGNETFGLSIEEVEQCNRLVTIPGNPDYFSLNLAMAVQVMTYELFSHT
GVDVEYLRAEGEAATQSEVDGMCGHLEQAMDEIGYFQRRNSERLMRRMRTMFHRAGMLREEIDILRGFYKQVQRTASGRS
GDAD

Sequences:

>Translated_244_residues
MQNIRVVLARPNHPGNIGSAARAMKTMGLSRLYLVEPKVFPSEEANALASGAVDLLENATVVGSLREALSDVTVACALTS
RRRELTTPLSTPRQTTPELIARARDGEQVALVFGNETFGLSIEEVEQCNRLVTIPGNPDYFSLNLAMAVQVMTYELFSHT
GVDVEYLRAEGEAATQSEVDGMCGHLEQAMDEIGYFQRRNSERLMRRMRTMFHRAGMLREEIDILRGFYKQVQRTASGRS
GDAD
>Mature_244_residues
MQNIRVVLARPNHPGNIGSAARAMKTMGLSRLYLVEPKVFPSEEANALASGAVDLLENATVVGSLREALSDVTVACALTS
RRRELTTPLSTPRQTTPELIARARDGEQVALVFGNETFGLSIEEVEQCNRLVTIPGNPDYFSLNLAMAVQVMTYELFSHT
GVDVEYLRAEGEAATQSEVDGMCGHLEQAMDEIGYFQRRNSERLMRRMRTMFHRAGMLREEIDILRGFYKQVQRTASGRS
GDAD

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=248, Percent_Identity=39.5161290322581, Blast_Score=174, Evalue=7e-45,
Organism=Escherichia coli, GI1790865, Length=153, Percent_Identity=38.562091503268, Blast_Score=98, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27058; Mature: 27058

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNIRVVLARPNHPGNIGSAARAMKTMGLSRLYLVEPKVFPSEEANALASGAVDLLENAT
CCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
VVGSLREALSDVTVACALTSRRRELTTPLSTPRQTTPELIARARDGEQVALVFGNETFGL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCC
SIEEVEQCNRLVTIPGNPDYFSLNLAMAVQVMTYELFSHTGVDVEYLRAEGEAATQSEVD
CHHHHHHCCCEEECCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
GMCGHLEQAMDEIGYFQRRNSERLMRRMRTMFHRAGMLREEIDILRGFYKQVQRTASGRS
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GDAD
CCCC
>Mature Secondary Structure
MQNIRVVLARPNHPGNIGSAARAMKTMGLSRLYLVEPKVFPSEEANALASGAVDLLENAT
CCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
VVGSLREALSDVTVACALTSRRRELTTPLSTPRQTTPELIARARDGEQVALVFGNETFGL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCC
SIEEVEQCNRLVTIPGNPDYFSLNLAMAVQVMTYELFSHTGVDVEYLRAEGEAATQSEVD
CHHHHHHCCCEEECCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
GMCGHLEQAMDEIGYFQRRNSERLMRRMRTMFHRAGMLREEIDILRGFYKQVQRTASGRS
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GDAD
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA