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The map label for this gene is metE

Identifier: 34499059

GI number: 34499059

Start: 3910140

End: 3912422

Strand: Direct

Name: metE

Synonym: CV_3604

Alternate gene names: 34499059

Gene position: 3910140-3912422 (Clockwise)

Preceding gene: 34499057

Following gene: 34499063

Centisome position: 82.3

GC content: 70.08

Gene sequence:

>2283_bases
ATGAGCCACGCCACCCACCTGCTGGGCTTCCCCCGCATCGGCGCCAAGCGCGAGCTGAAAACGCTGCTGGAGCGATACTG
GAAACAGGAACTGGACGAAGCCGCGCTGTCGCAGGGCGCGAAAGAGCTGCGCCAGAAGCACTGGCTGCTGCAGAAAGGCG
CCGGCGTCGAACTGAGCCCGGTCGGCGACTTCTCGCTGTACGACCACGTGCTGGACGCGCAGCTGCTGGTCGGCGCCGCG
CCGGCGCGCTTCGGCTTCGACGCCGCCGCGCTCACCACCGGCCAGTATTTCGAACTGGCGCGCGGCAACGCCGTTCAGCC
TGCGATGGAAATGACCAAGTGGTTCGATACCAACTACCACTACCTGGTGCCGGAATGGCATGCCGACACCGCCTTCTCCG
CCCAGCCCGAGCGGCTGCTGTCGCAGTTGCGCGAGGCCCGGGCGCTGGGCGTGGCCGCCAAGCCCGTGCTGCTGGGGCCG
CTCAGCCTGCTATGGCTGGGCAAGGCCAAGGGCCGGCCTTTCGACAGGCTCGCGCTGCTGCCCGGCCTGGTCGCCGCCTA
TCGCGAGCTGCTGTCGTCGCTGCGCGCCGCCGGCGCGGAGTGGGCGCAGATCGATGAACCCATCCTGGCGCTGGACCTGG
AGCCGTCGTGGCTGGACGCCTTCGCGCCAGCTTACGCGCAATTGAGCCGGCATGCGCCCAAGCTGCTGCTGGCCACCTAT
TTCGGCGATGTGTCCGAACACGCGGCGCGGCTGAAGTCGCTGCCGGTGGCCGGCCTGCACCTGGACCTGGTCCGCGCGCC
GGAACAGATTTCCGCCTTCCTGCCGGACTATCCGGCCGACAAGGTGCTGTCCGCCGGCATCGTCGACGGCCGCAACATCT
GGCGCGCCGACCTGTCCGCGCTGCTGGACCGCCTGGCCCCGCTGGCCCAGCAACTGGGCGACAGGCTGTGGCTGGCCCCC
AGCTGCTCGCTGCTGCACAGCCCCTTCGACGCCGCCGCCGAAACCGGGCTGGACCCGGAGCTGAAAAACTGGCTGGCCTT
CGCGGTGCAGAAACTCAACGAGCTCAAGACGCTGAAGCGCGGACTGGAGCACGGCCGCTCCGCCATCGCCGGCGAACTAT
CGGGCAGCGATTTCGCCCGCGAGCAGCGCCGAGCCAGCCCCCGCATCCATGACCCGGCCGTCGCGCGGCGCCTGGCCGCG
CTGCCGGAAGGCACCGACCGCCGCGCCAGCCCCTACCCCATCCGCGCCGAGCGGCAGCAAGCCTGGCTCAAGCTGCCGCC
GCTGCCGACCACCACCATCGGCTCCTTCCCGCAAACGCCGGCCATCCGCGCCAGCCGCGCCGCGTTCAAGAAGGGGGAAC
TGTCCGCCGCCGGCTATCGGCAGGCGATGGAGAAGGAAATCGAGCTGGCGATCCGCCGCCAGGAGGCGCTGGGGCTGGAC
GTGCTGGTGCATGGCGAGGCCGAGCGCAACGACATGGTGGAATACTTCGGCGAGCAGCTGGCCGGCTTCGCCTTCACCGC
CGGCGGCTGGGTGCAGAGCTACGGCAGCCGCTGCGTGAAGCCGCCCATCATCTTCGGCGACGTGGCGCGGCCCAAGCCCA
TGACCGTGGACTGGGCGCGCTACGCGCAAAGCCTGACCGCCAAACCGGTGAAGGGCATGCTGACTGGTCCGGTCACCATC
CTGCAATGGAGCTTCGTCCGCGACGACCTGCCCAGGCGCGAAGTCTGCCGCCAGATCGCGCTGGCGCTGAACGACGAGGT
GCTCGACCTGGAGGCCGCCGGCATCCGCGTGATCCAGATCGACGAGCCCGCCATCCGCGAAGGCCTGCCGCTGAAGCGCG
CCGGCCGCGACGTCTACCTGGCCTGGGCCGGCGAGGCCTTCCGTCTGTCCAGCCGCGGCGTGGACGACGCGACGCAGATC
CATACCCACATGTGCTACTCGGAATTCGGCGACATCCTGCCGGCCATCGCCGCGCTGGACGCCGACGTGATCACCATCGA
AACTTCGCGCTCGGACATGGCGCTGCTCGCCGACTTCGGCCGCTTCCGCTATCCGAACGCGATCGGGCCCGGCGTCTACG
ACATCCACAGCCCGCGCGTGCCGTCCGCCGCCGAAATCCGCGCGCTGCTGGACAAGGCGCTGCGGGTGATTCCGGCCGAG
CGCCTGTGGGTGAATCCAGACTGCGGGCTGAAAACCCGCGGCTGGCCCGAGGTGGAGGCCGCACTGGCGGCGATGGTGGC
CGTCGGCCGCGAGCTGCGCGAAAAGCTGGCACAGGCGGCCTGA

Upstream 100 bases:

>100_bases
TCATCGATTCCATTCATGTTGAACAACAAAATAATGAGTTTGAATCATACACCGCAATGACAGATGCTTGTTCCATCCCG
ACAAGAACAGGAACGCACCC

Downstream 100 bases:

>100_bases
GGCGGAAACGAAGACGCCCCGGCATGCCGGGGCGTCGGACAGGGACAGGGGGAAGGTTCGGGACTCACGGCTCGAGCATC
CCCGGCGTCAGCATCAGCTT

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 760; Mature: 759

Protein sequence:

>760_residues
MSHATHLLGFPRIGAKRELKTLLERYWKQELDEAALSQGAKELRQKHWLLQKGAGVELSPVGDFSLYDHVLDAQLLVGAA
PARFGFDAAALTTGQYFELARGNAVQPAMEMTKWFDTNYHYLVPEWHADTAFSAQPERLLSQLREARALGVAAKPVLLGP
LSLLWLGKAKGRPFDRLALLPGLVAAYRELLSSLRAAGAEWAQIDEPILALDLEPSWLDAFAPAYAQLSRHAPKLLLATY
FGDVSEHAARLKSLPVAGLHLDLVRAPEQISAFLPDYPADKVLSAGIVDGRNIWRADLSALLDRLAPLAQQLGDRLWLAP
SCSLLHSPFDAAAETGLDPELKNWLAFAVQKLNELKTLKRGLEHGRSAIAGELSGSDFAREQRRASPRIHDPAVARRLAA
LPEGTDRRASPYPIRAERQQAWLKLPPLPTTTIGSFPQTPAIRASRAAFKKGELSAAGYRQAMEKEIELAIRRQEALGLD
VLVHGEAERNDMVEYFGEQLAGFAFTAGGWVQSYGSRCVKPPIIFGDVARPKPMTVDWARYAQSLTAKPVKGMLTGPVTI
LQWSFVRDDLPRREVCRQIALALNDEVLDLEAAGIRVIQIDEPAIREGLPLKRAGRDVYLAWAGEAFRLSSRGVDDATQI
HTHMCYSEFGDILPAIAALDADVITIETSRSDMALLADFGRFRYPNAIGPGVYDIHSPRVPSAAEIRALLDKALRVIPAE
RLWVNPDCGLKTRGWPEVEAALAAMVAVGRELREKLAQAA

Sequences:

>Translated_760_residues
MSHATHLLGFPRIGAKRELKTLLERYWKQELDEAALSQGAKELRQKHWLLQKGAGVELSPVGDFSLYDHVLDAQLLVGAA
PARFGFDAAALTTGQYFELARGNAVQPAMEMTKWFDTNYHYLVPEWHADTAFSAQPERLLSQLREARALGVAAKPVLLGP
LSLLWLGKAKGRPFDRLALLPGLVAAYRELLSSLRAAGAEWAQIDEPILALDLEPSWLDAFAPAYAQLSRHAPKLLLATY
FGDVSEHAARLKSLPVAGLHLDLVRAPEQISAFLPDYPADKVLSAGIVDGRNIWRADLSALLDRLAPLAQQLGDRLWLAP
SCSLLHSPFDAAAETGLDPELKNWLAFAVQKLNELKTLKRGLEHGRSAIAGELSGSDFAREQRRASPRIHDPAVARRLAA
LPEGTDRRASPYPIRAERQQAWLKLPPLPTTTIGSFPQTPAIRASRAAFKKGELSAAGYRQAMEKEIELAIRRQEALGLD
VLVHGEAERNDMVEYFGEQLAGFAFTAGGWVQSYGSRCVKPPIIFGDVARPKPMTVDWARYAQSLTAKPVKGMLTGPVTI
LQWSFVRDDLPRREVCRQIALALNDEVLDLEAAGIRVIQIDEPAIREGLPLKRAGRDVYLAWAGEAFRLSSRGVDDATQI
HTHMCYSEFGDILPAIAALDADVITIETSRSDMALLADFGRFRYPNAIGPGVYDIHSPRVPSAAEIRALLDKALRVIPAE
RLWVNPDCGLKTRGWPEVEAALAAMVAVGRELREKLAQAA
>Mature_759_residues
SHATHLLGFPRIGAKRELKTLLERYWKQELDEAALSQGAKELRQKHWLLQKGAGVELSPVGDFSLYDHVLDAQLLVGAAP
ARFGFDAAALTTGQYFELARGNAVQPAMEMTKWFDTNYHYLVPEWHADTAFSAQPERLLSQLREARALGVAAKPVLLGPL
SLLWLGKAKGRPFDRLALLPGLVAAYRELLSSLRAAGAEWAQIDEPILALDLEPSWLDAFAPAYAQLSRHAPKLLLATYF
GDVSEHAARLKSLPVAGLHLDLVRAPEQISAFLPDYPADKVLSAGIVDGRNIWRADLSALLDRLAPLAQQLGDRLWLAPS
CSLLHSPFDAAAETGLDPELKNWLAFAVQKLNELKTLKRGLEHGRSAIAGELSGSDFAREQRRASPRIHDPAVARRLAAL
PEGTDRRASPYPIRAERQQAWLKLPPLPTTTIGSFPQTPAIRASRAAFKKGELSAAGYRQAMEKEIELAIRRQEALGLDV
LVHGEAERNDMVEYFGEQLAGFAFTAGGWVQSYGSRCVKPPIIFGDVARPKPMTVDWARYAQSLTAKPVKGMLTGPVTIL
QWSFVRDDLPRREVCRQIALALNDEVLDLEAAGIRVIQIDEPAIREGLPLKRAGRDVYLAWAGEAFRLSSRGVDDATQIH
THMCYSEFGDILPAIAALDADVITIETSRSDMALLADFGRFRYPNAIGPGVYDIHSPRVPSAAEIRALLDKALRVIPAER
LWVNPDCGLKTRGWPEVEAALAAMVAVGRELREKLAQAA

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=758, Percent_Identity=56.8601583113456, Blast_Score=840, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=768, Percent_Identity=48.6979166666667, Blast_Score=698, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_CHRVO (Q7NS23)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903274.1
- ProteinModelPortal:   Q7NS23
- SMR:   Q7NS23
- GeneID:   2548455
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3604
- NMPDR:   fig|243365.1.peg.3604
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- PhylomeDB:   Q7NS23
- ProtClustDB:   PRK05222
- BioCyc:   CVIO243365:CV_3604-MONOMER
- BRENDA:   2.1.1.14
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 83582; Mature: 83451

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHATHLLGFPRIGAKRELKTLLERYWKQELDEAALSQGAKELRQKHWLLQKGAGVELSP
CCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECC
VGDFSLYDHVLDAQLLVGAAPARFGFDAAALTTGQYFELARGNAVQPAMEMTKWFDTNYH
CCCHHHHHHHHHHHHHHCCCCHHCCCCHHHHCCCHHHHHHCCCCCCHHHHHHHHHCCCCE
YLVPEWHADTAFSAQPERLLSQLREARALGVAAKPVLLGPLSLLWLGKAKGRPFDRLALL
EECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHCCCCCCCCHHHHHHH
PGLVAAYRELLSSLRAAGAEWAQIDEPILALDLEPSWLDAFAPAYAQLSRHAPKLLLATY
HHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHH
FGDVSEHAARLKSLPVAGLHLDLVRAPEQISAFLPDYPADKVLSAGIVDGRNIWRADLSA
HCCHHHHHHHHHHCCCCCEEHHHHHCHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHH
LLDRLAPLAQQLGDRLWLAPSCSLLHSPFDAAAETGLDPELKNWLAFAVQKLNELKTLKR
HHHHHHHHHHHHCCCEEECCCHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
GLEHGRSAIAGELSGSDFAREQRRASPRIHDPAVARRLAALPEGTDRRASPYPIRAERQQ
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
AWLKLPPLPTTTIGSFPQTPAIRASRAAFKKGELSAAGYRQAMEKEIELAIRRQEALGLD
CCEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
VLVHGEAERNDMVEYFGEQLAGFAFTAGGWVQSYGSRCVKPPIIFGDVARPKPMTVDWAR
EEEECCCCHHHHHHHHHHHHHCCEEECCHHHHHHCCCCCCCCEEECCCCCCCCCEEHHHH
YAQSLTAKPVKGMLTGPVTILQWSFVRDDLPRREVCRQIALALNDEVLDLEAAGIRVIQI
HHHHHCCCCCCCHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCEEEEEE
DEPAIREGLPLKRAGRDVYLAWAGEAFRLSSRGVDDATQIHTHMCYSEFGDILPAIAALD
CCHHHHHCCCHHHCCCEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
ADVITIETSRSDMALLADFGRFRYPNAIGPGVYDIHSPRVPSAAEIRALLDKALRVIPAE
CCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCH
RLWVNPDCGLKTRGWPEVEAALAAMVAVGRELREKLAQAA
HEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SHATHLLGFPRIGAKRELKTLLERYWKQELDEAALSQGAKELRQKHWLLQKGAGVELSP
CCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECC
VGDFSLYDHVLDAQLLVGAAPARFGFDAAALTTGQYFELARGNAVQPAMEMTKWFDTNYH
CCCHHHHHHHHHHHHHHCCCCHHCCCCHHHHCCCHHHHHHCCCCCCHHHHHHHHHCCCCE
YLVPEWHADTAFSAQPERLLSQLREARALGVAAKPVLLGPLSLLWLGKAKGRPFDRLALL
EECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHCCCCCCCCHHHHHHH
PGLVAAYRELLSSLRAAGAEWAQIDEPILALDLEPSWLDAFAPAYAQLSRHAPKLLLATY
HHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHH
FGDVSEHAARLKSLPVAGLHLDLVRAPEQISAFLPDYPADKVLSAGIVDGRNIWRADLSA
HCCHHHHHHHHHHCCCCCEEHHHHHCHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHH
LLDRLAPLAQQLGDRLWLAPSCSLLHSPFDAAAETGLDPELKNWLAFAVQKLNELKTLKR
HHHHHHHHHHHHCCCEEECCCHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
GLEHGRSAIAGELSGSDFAREQRRASPRIHDPAVARRLAALPEGTDRRASPYPIRAERQQ
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
AWLKLPPLPTTTIGSFPQTPAIRASRAAFKKGELSAAGYRQAMEKEIELAIRRQEALGLD
CCEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
VLVHGEAERNDMVEYFGEQLAGFAFTAGGWVQSYGSRCVKPPIIFGDVARPKPMTVDWAR
EEEECCCCHHHHHHHHHHHHHCCEEECCHHHHHHCCCCCCCCEEECCCCCCCCCEEHHHH
YAQSLTAKPVKGMLTGPVTILQWSFVRDDLPRREVCRQIALALNDEVLDLEAAGIRVIQI
HHHHHCCCCCCCHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCEEEEEE
DEPAIREGLPLKRAGRDVYLAWAGEAFRLSSRGVDDATQIHTHMCYSEFGDILPAIAALD
CCHHHHHCCCHHHCCCEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
ADVITIETSRSDMALLADFGRFRYPNAIGPGVYDIHSPRVPSAAEIRALLDKALRVIPAE
CCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCH
RLWVNPDCGLKTRGWPEVEAALAAMVAVGRELREKLAQAA
HEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782