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The map label for this gene is aroE

Identifier: 34499042

GI number: 34499042

Start: 3895223

End: 3896044

Strand: Reverse

Name: aroE

Synonym: CV_3587

Alternate gene names: 34499042

Gene position: 3896044-3895223 (Counterclockwise)

Preceding gene: 34499043

Following gene: 34499041

Centisome position: 82.0

GC content: 67.03

Gene sequence:

>822_bases
ATGACCGACCGTTACGCCGTAATCGGCAACCCGATCTCCCACAGCCAGTCTCCGTTCATACACGAGGAGTTCGCCCGCGC
CACCGGGCAGGACATCAGCTACGAGCGCCTGTTCGCCGACATCGGCCGCTTCAACGACGTGGTGGGCGAGTTCGTCGCCA
GCGGCGGCAAGGGCTTGAACATCACGCTGCCGTTCAAGGGCGACGCTTTCCGCTACGCGTCCGAGCTGACCGAGCGCGCG
CGCGCGGCCGAGGCGGTCAACACGCTGACTTTCCGCGACGGCAAGGTATACGGCGACAACACCGACGGCGTCGGCCTGGT
GCGCGACATCGTGGAAAACCTCGACTACCCGATCGTTGGACGACGCGTGCTGATCCTGGGTGCCGGCGGCGCGGTGCGCG
GCGTGCTGGAGCCCATCCTGGAGCAGAAGCCGGCCAGCCTGACCATCGCCAACCGCACCGTGATCAAGGCCGAGGCGCTG
GCCCATCATTTCGCCCGCTACGGCAAAGTGGAGGCCGTCGGCTACGCCGCGCTGGAAGGCCGCAGCTTCGACATCGTCAT
CAACGCCACCTCCACCAGCCTGAACAACGAGATGCCGCCGCTGCCGCACGGCGTGTTCACGCCGCGCACGCTGGCCTACG
ACATGGTGTACAGCACCGGCCTGACGCCGTTCCTGCAGCGCGCCCAAGGCGAAAACGCCGGCATGCTGGCCGACGGGCTG
GGCATGCTGGTGGAGCAGGCGGCGGAATCGTTCTCCATCTGGCGCGGCGCGCAGCCGGAAACGCGCAAGGTGACCAATAT
GCTGAGGGAAGTGCTGGCCTGA

Upstream 100 bases:

>100_bases
GGTTCGCTGAACGGTCTTCTTCTGTCCGCCGCGCGGGCGCGGGAGGCCGCCTCGGCGCTTCCTGGCCGCAGAAAACATAT
CGCATTTTGGAAAGCCGGAC

Downstream 100 bases:

>100_bases
GCATGTCGCGCATCCTGTTCAAGATCCTGGCGGCCCTGGTGGCCGCCTTTCTGCTGTATAACCTGTGGGTGCTCGGCCAT
ATCATTTACTGGCGCGACCA

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MTDRYAVIGNPISHSQSPFIHEEFARATGQDISYERLFADIGRFNDVVGEFVASGGKGLNITLPFKGDAFRYASELTERA
RAAEAVNTLTFRDGKVYGDNTDGVGLVRDIVENLDYPIVGRRVLILGAGGAVRGVLEPILEQKPASLTIANRTVIKAEAL
AHHFARYGKVEAVGYAALEGRSFDIVINATSTSLNNEMPPLPHGVFTPRTLAYDMVYSTGLTPFLQRAQGENAGMLADGL
GMLVEQAAESFSIWRGAQPETRKVTNMLREVLA

Sequences:

>Translated_273_residues
MTDRYAVIGNPISHSQSPFIHEEFARATGQDISYERLFADIGRFNDVVGEFVASGGKGLNITLPFKGDAFRYASELTERA
RAAEAVNTLTFRDGKVYGDNTDGVGLVRDIVENLDYPIVGRRVLILGAGGAVRGVLEPILEQKPASLTIANRTVIKAEAL
AHHFARYGKVEAVGYAALEGRSFDIVINATSTSLNNEMPPLPHGVFTPRTLAYDMVYSTGLTPFLQRAQGENAGMLADGL
GMLVEQAAESFSIWRGAQPETRKVTNMLREVLA
>Mature_272_residues
TDRYAVIGNPISHSQSPFIHEEFARATGQDISYERLFADIGRFNDVVGEFVASGGKGLNITLPFKGDAFRYASELTERAR
AAEAVNTLTFRDGKVYGDNTDGVGLVRDIVENLDYPIVGRRVLILGAGGAVRGVLEPILEQKPASLTIANRTVIKAEALA
HHFARYGKVEAVGYAALEGRSFDIVINATSTSLNNEMPPLPHGVFTPRTLAYDMVYSTGLTPFLQRAQGENAGMLADGLG
MLVEQAAESFSIWRGAQPETRKVTNMLREVLA

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1789675, Length=272, Percent_Identity=47.7941176470588, Blast_Score=247, Evalue=7e-67,
Organism=Escherichia coli, GI1787983, Length=282, Percent_Identity=30.8510638297872, Blast_Score=96, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320332, Length=268, Percent_Identity=27.9850746268657, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_CHRVO (Q7NS40)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903257.1
- HSSP:   P15770
- ProteinModelPortal:   Q7NS40
- SMR:   Q7NS40
- GeneID:   2548870
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3587
- NMPDR:   fig|243365.1.peg.3587
- HOGENOM:   HBG553408
- OMA:   ELAYGKG
- PhylomeDB:   Q7NS40
- ProtClustDB:   CLSK2485629
- BioCyc:   CVIO243365:CV_3587-MONOMER
- BRENDA:   1.1.1.25
- GO:   GO:0005737
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 29650; Mature: 29519

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: ACT_SITE 66-66

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRYAVIGNPISHSQSPFIHEEFARATGQDISYERLFADIGRFNDVVGEFVASGGKGLN
CCCCEEEECCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEE
ITLPFKGDAFRYASELTERARAAEAVNTLTFRDGKVYGDNTDGVGLVRDIVENLDYPIVG
EEECCCCCHHHHHHHHHHHHHHHHHHHHEEEECCEEECCCCCCHHHHHHHHHHCCCCCCC
RRVLILGAGGAVRGVLEPILEQKPASLTIANRTVIKAEALAHHFARYGKVEAVGYAALEG
CEEEEEECCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCHHEEEEEEECC
RSFDIVINATSTSLNNEMPPLPHGVFTPRTLAYDMVYSTGLTPFLQRAQGENAGMLADGL
CEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHH
GMLVEQAAESFSIWRGAQPETRKVTNMLREVLA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHC
>Mature Secondary Structure 
TDRYAVIGNPISHSQSPFIHEEFARATGQDISYERLFADIGRFNDVVGEFVASGGKGLN
CCCEEEECCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEE
ITLPFKGDAFRYASELTERARAAEAVNTLTFRDGKVYGDNTDGVGLVRDIVENLDYPIVG
EEECCCCCHHHHHHHHHHHHHHHHHHHHEEEECCEEECCCCCCHHHHHHHHHHCCCCCCC
RRVLILGAGGAVRGVLEPILEQKPASLTIANRTVIKAEALAHHFARYGKVEAVGYAALEG
CEEEEEECCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCHHEEEEEEECC
RSFDIVINATSTSLNNEMPPLPHGVFTPRTLAYDMVYSTGLTPFLQRAQGENAGMLADGL
CEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHH
GMLVEQAAESFSIWRGAQPETRKVTNMLREVLA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782