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The map label for this gene is hemK [H]

Identifier: 34499039

GI number: 34499039

Start: 3892043

End: 3892807

Strand: Reverse

Name: hemK [H]

Synonym: CV_3584

Alternate gene names: 34499039

Gene position: 3892807-3892043 (Counterclockwise)

Preceding gene: 34499040

Following gene: 34499038

Centisome position: 81.94

GC content: 71.76

Gene sequence:

>765_bases
ATGCTGCTGACGCAGGCCTGGCCCGGCCTGACCCACGCCACCATCCTCGGCCATCCCGAGCGCGAGCTGCCGGAGGAGAC
TGCGTCCGCTTTCGACGCGCTGGCCGCGCGGCGCCTGGCCGGCGAGCCGATCGCCTACCTGCTGGGATCGCGCGAATTCT
ACGGCCGGGATTTCCGGGTGAGCCCGGCGGTGCTGATTCCGCGGCCGGAAACCGAACATCTGGTCGAGCAGGCGCTGTCC
CGCGTCGACCGTACCATGCCGGCTCGGGCGGTGGATCTGGGCACCGGCAGCGGCATCATCGCCATCACGCTGGCGCTGGA
GGCGCCGGACTGGTCGTTGGGCGCGGTCGATGTCTCGGCCGAGGCGCTGGCCGTCGCGCGCGGCAATGCCGACGCGCTGG
GCGCCCGCGTCGATTTCCGCCTGGGCAGCTGGTTCGGCCCGCTGGACGCGGACGCGCGTTTCGAGCTGATCGTGTCCAAC
CCGCCCTACATCGAGCGCGGCGACCATCATCTGGCGGAAGGCGACCTGCGTTTCGAGCCGCGCGGCGCGTTGACCGACGA
GGCCGACGGCCTGGCCTGCCTGCGCGAAATCGCCGCCGGCGCGCCGGGGCGGCTGGCCGACGGCGGCTGGCTGATGGTCG
AGCACGGCTACGACCAGGGCGAGGCGGTGCGGGGCTTGTTCGCCGACGCCGGCTTGCTTGAAGTGGAAACCATTGTCGAT
CTGGCGGGGCAGGACCGCATCACCATCGGGCGGAAGGCGGTTTGA

Upstream 100 bases:

>100_bases
ATTTTCCTGGGGCTGGCCACCCTGTTCCTGCTGTAATAGAACCATGCCGACCATAGAACAGGCGCTGCGGCGCCACCCCT
TGCCCAGGCTGGAGTCCAGG

Downstream 100 bases:

>100_bases
GCGATGTCCCGGCTTAGCCGATTCTGGCCGGCCATGCTGTTGGCCGCATTGAGCGCGGCCCTGCTGGCCTGGCCGCAGGC
CAACCGCCTGCTGTTTCTTT

Product: HemK protein

Products: NA

Alternate protein names: M.VchAHemK2P [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MLLTQAWPGLTHATILGHPERELPEETASAFDALAARRLAGEPIAYLLGSREFYGRDFRVSPAVLIPRPETEHLVEQALS
RVDRTMPARAVDLGTGSGIIAITLALEAPDWSLGAVDVSAEALAVARGNADALGARVDFRLGSWFGPLDADARFELIVSN
PPYIERGDHHLAEGDLRFEPRGALTDEADGLACLREIAAGAPGRLADGGWLMVEHGYDQGEAVRGLFADAGLLEVETIVD
LAGQDRITIGRKAV

Sequences:

>Translated_254_residues
MLLTQAWPGLTHATILGHPERELPEETASAFDALAARRLAGEPIAYLLGSREFYGRDFRVSPAVLIPRPETEHLVEQALS
RVDRTMPARAVDLGTGSGIIAITLALEAPDWSLGAVDVSAEALAVARGNADALGARVDFRLGSWFGPLDADARFELIVSN
PPYIERGDHHLAEGDLRFEPRGALTDEADGLACLREIAAGAPGRLADGGWLMVEHGYDQGEAVRGLFADAGLLEVETIVD
LAGQDRITIGRKAV
>Mature_254_residues
MLLTQAWPGLTHATILGHPERELPEETASAFDALAARRLAGEPIAYLLGSREFYGRDFRVSPAVLIPRPETEHLVEQALS
RVDRTMPARAVDLGTGSGIIAITLALEAPDWSLGAVDVSAEALAVARGNADALGARVDFRLGSWFGPLDADARFELIVSN
PPYIERGDHHLAEGDLRFEPRGALTDEADGLACLREIAAGAPGRLADGGWLMVEHGYDQGEAVRGLFADAGLLEVETIVD
LAGQDRITIGRKAV

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=256, Percent_Identity=30.46875, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1787463, Length=238, Percent_Identity=47.8991596638655, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI87082085, Length=181, Percent_Identity=35.3591160220994, Blast_Score=97, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6324266, Length=182, Percent_Identity=30.2197802197802, Blast_Score=69, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24582226, Length=208, Percent_Identity=28.8461538461538, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 27150; Mature: 27150

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLTQAWPGLTHATILGHPERELPEETASAFDALAARRLAGEPIAYLLGSREFYGRDFRV
CCCCCCCCCCCEEEEECCCHHHCHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHCCCCEEC
SPAVLIPRPETEHLVEQALSRVDRTMPARAVDLGTGSGIIAITLALEAPDWSLGAVDVSA
CCEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEECCCCCCEEEECCC
EALAVARGNADALGARVDFRLGSWFGPLDADARFELIVSNPPYIERGDHHLAEGDLRFEP
CEEEEECCCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCEEECCCEEECC
RGALTDEADGLACLREIAAGAPGRLADGGWLMVEHGYDQGEAVRGLFADAGLLEVETIVD
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHH
LAGQDRITIGRKAV
HCCCCEEECCCCCC
>Mature Secondary Structure
MLLTQAWPGLTHATILGHPERELPEETASAFDALAARRLAGEPIAYLLGSREFYGRDFRV
CCCCCCCCCCCEEEEECCCHHHCHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHCCCCEEC
SPAVLIPRPETEHLVEQALSRVDRTMPARAVDLGTGSGIIAITLALEAPDWSLGAVDVSA
CCEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEECCCCCCEEEECCC
EALAVARGNADALGARVDFRLGSWFGPLDADARFELIVSNPPYIERGDHHLAEGDLRFEP
CEEEEECCCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCEEECCCEEECC
RGALTDEADGLACLREIAAGAPGRLADGGWLMVEHGYDQGEAVRGLFADAGLLEVETIVD
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHH
LAGQDRITIGRKAV
HCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]