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The map label for this gene is cysK [H]

Identifier: 34499016

GI number: 34499016

Start: 3867639

End: 3868571

Strand: Reverse

Name: cysK [H]

Synonym: CV_3561

Alternate gene names: 34499016

Gene position: 3868571-3867639 (Counterclockwise)

Preceding gene: 34499018

Following gene: 34499015

Centisome position: 81.43

GC content: 66.45

Gene sequence:

>933_bases
ATGCGCATCGCCAACACCATCACCGATCTGATCGGCAACACCCCGCTGGTCAAGCTCAACCGCGTCACCGAGGGCGTCGG
CGCCACCGTCGTCGCCAAGCTGGAGTTCTTCAATCCGGCGCACAGCGTCAAGGACCGCATCGCGGTGGCGATGCTGGACG
CGGCGGAGCAAGCCGGCAAGATCGGTCCGAACACCGTCATCGTCGAACCCACCTCGGGCAATACCGGCATCGGCCTGGCG
ATGGTGTGCGCGGCGCGCGGCTACAAGCTGGTGATCACCATGCCGGAAACCATGAGCAAGGAGCGCCGCCAGCTGTTGAA
GGCCTACGGCGCCGAACTGATCCTGACCCCGGGGCCGGACGGCATGGGCGGCGCCATCGCCAAGGCGAAGGCGCTGGTCG
ACGAATTTCCCGACACCTACTTCATGCCGCAGCAGTTTGAAAACCCGGCCAACCCGGAAATCCACCGCCACACCACCGCG
GAAGAGGTGTGGAACGACACCGACGGCAAGGTGGACATCTTCGTGGCCGGGGTCGGCACCGGCGGCACCATCACCGGGGT
CGGCGAGGTGTTGAAGGCGCGCAAGCCGGGCGTGCAGATCGTCGCGGTGGAGCCGGACGCGTCGGCGGTGCTGTCCGGCA
GCCCCAAGGGCCCGCACCCGATCCAGGGCATAGGCGCGGGCTTCGTGCCAGCCATCCTCAATACCGGCATTTACGACGAG
ATCATCCGCGTCAAGAACGAGGACGCGCTGCAGACCGCGCGCGAGGTGGCCACCAAGGAAGGCATCCTGGTCGGCATCTC
GTCCGGCGCGGCGGTGTGGTCGGCGCTGCAGCTGGCCAAACGGCCGGAAAACGCCGGCAAGCTGATCGTAGTGGTGATCC
CGTCGTTCGGCGAGCGTTACCTGTCCACCGTGCTGTTCGAGCATCTTGGCTGA

Upstream 100 bases:

>100_bases
TGCTGGATATAAGCAAACAATATTAGGTCATCCGCGCCGGATTGATTATGATGCCGTGGAATATCGGCATTGTCATTTTA
GCTGGATGAGGAATAAGATC

Downstream 100 bases:

>100_bases
GCGGTCGCCGGATTCGGCCCGCCGGCCCGCGCCGCGCGCAATCGATCGCAATACCTTTGCCGTTGACGGCGCGCGGGCAG
GGCGGGCTACAATGGCCGCC

Product: cysteine synthase

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MRIANTITDLIGNTPLVKLNRVTEGVGATVVAKLEFFNPAHSVKDRIAVAMLDAAEQAGKIGPNTVIVEPTSGNTGIGLA
MVCAARGYKLVITMPETMSKERRQLLKAYGAELILTPGPDGMGGAIAKAKALVDEFPDTYFMPQQFENPANPEIHRHTTA
EEVWNDTDGKVDIFVAGVGTGGTITGVGEVLKARKPGVQIVAVEPDASAVLSGSPKGPHPIQGIGAGFVPAILNTGIYDE
IIRVKNEDALQTAREVATKEGILVGISSGAAVWSALQLAKRPENAGKLIVVVIPSFGERYLSTVLFEHLG

Sequences:

>Translated_310_residues
MRIANTITDLIGNTPLVKLNRVTEGVGATVVAKLEFFNPAHSVKDRIAVAMLDAAEQAGKIGPNTVIVEPTSGNTGIGLA
MVCAARGYKLVITMPETMSKERRQLLKAYGAELILTPGPDGMGGAIAKAKALVDEFPDTYFMPQQFENPANPEIHRHTTA
EEVWNDTDGKVDIFVAGVGTGGTITGVGEVLKARKPGVQIVAVEPDASAVLSGSPKGPHPIQGIGAGFVPAILNTGIYDE
IIRVKNEDALQTAREVATKEGILVGISSGAAVWSALQLAKRPENAGKLIVVVIPSFGERYLSTVLFEHLG
>Mature_310_residues
MRIANTITDLIGNTPLVKLNRVTEGVGATVVAKLEFFNPAHSVKDRIAVAMLDAAEQAGKIGPNTVIVEPTSGNTGIGLA
MVCAARGYKLVITMPETMSKERRQLLKAYGAELILTPGPDGMGGAIAKAKALVDEFPDTYFMPQQFENPANPEIHRHTTA
EEVWNDTDGKVDIFVAGVGTGGTITGVGEVLKARKPGVQIVAVEPDASAVLSGSPKGPHPIQGIGAGFVPAILNTGIYDE
IIRVKNEDALQTAREVATKEGILVGISSGAAVWSALQLAKRPENAGKLIVVVIPSFGERYLSTVLFEHLG

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=314, Percent_Identity=38.5350318471338, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI295821200, Length=314, Percent_Identity=38.5350318471338, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI4557415, Length=314, Percent_Identity=38.5350318471338, Blast_Score=227, Evalue=1e-59,
Organism=Escherichia coli, GI1788754, Length=313, Percent_Identity=54.9520766773163, Blast_Score=293, Evalue=9e-81,
Organism=Escherichia coli, GI2367138, Length=307, Percent_Identity=42.671009771987, Blast_Score=234, Evalue=5e-63,
Organism=Caenorhabditis elegans, GI17535051, Length=302, Percent_Identity=55.2980132450331, Blast_Score=334, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI17562970, Length=299, Percent_Identity=53.5117056856187, Blast_Score=321, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI32566674, Length=294, Percent_Identity=54.0816326530612, Blast_Score=317, Evalue=4e-87,
Organism=Caenorhabditis elegans, GI115535073, Length=298, Percent_Identity=56.0402684563758, Blast_Score=309, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI17561720, Length=302, Percent_Identity=52.317880794702, Blast_Score=289, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI32566672, Length=213, Percent_Identity=53.5211267605634, Blast_Score=234, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI17534315, Length=320, Percent_Identity=40.9375, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI25147552, Length=318, Percent_Identity=38.9937106918239, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17561716, Length=119, Percent_Identity=52.1008403361345, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321594, Length=317, Percent_Identity=37.2239747634069, Blast_Score=197, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6321449, Length=335, Percent_Identity=33.4328358208955, Blast_Score=142, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24643623, Length=317, Percent_Identity=38.4858044164038, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI20129101, Length=317, Percent_Identity=38.4858044164038, Blast_Score=197, Evalue=1e-50,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32625; Mature: 32625

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00901 CYS_SYNTHASE ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIANTITDLIGNTPLVKLNRVTEGVGATVVAKLEFFNPAHSVKDRIAVAMLDAAEQAGK
CCHHHHHHHHHCCCCEEEEECHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCC
IGPNTVIVEPTSGNTGIGLAMVCAARGYKLVITMPETMSKERRQLLKAYGAELILTPGPD
CCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEECCCC
GMGGAIAKAKALVDEFPDTYFMPQQFENPANPEIHRHTTAEEVWNDTDGKVDIFVAGVGT
CCCHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCHHHHHCCCCCEEEEEEEECCC
GGTITGVGEVLKARKPGVQIVAVEPDASAVLSGSPKGPHPIQGIGAGFVPAILNTGIYDE
CCCHHHHHHHHHCCCCCEEEEEECCCCCEEECCCCCCCCCCCCCCCCHHHHHHCCCHHHH
IIRVKNEDALQTAREVATKEGILVGISSGAAVWSALQLAKRPENAGKLIVVVIPSFGERY
HHHCCCCHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHH
LSTVLFEHLG
HHHHHHHHCC
>Mature Secondary Structure
MRIANTITDLIGNTPLVKLNRVTEGVGATVVAKLEFFNPAHSVKDRIAVAMLDAAEQAGK
CCHHHHHHHHHCCCCEEEEECHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCC
IGPNTVIVEPTSGNTGIGLAMVCAARGYKLVITMPETMSKERRQLLKAYGAELILTPGPD
CCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEECCCC
GMGGAIAKAKALVDEFPDTYFMPQQFENPANPEIHRHTTAEEVWNDTDGKVDIFVAGVGT
CCCHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCHHHHHCCCCCEEEEEEEECCC
GGTITGVGEVLKARKPGVQIVAVEPDASAVLSGSPKGPHPIQGIGAGFVPAILNTGIYDE
CCCHHHHHHHHHCCCCCEEEEEECCCCCEEECCCCCCCCCCCCCCCCHHHHHHCCCHHHH
IIRVKNEDALQTAREVATKEGILVGISSGAAVWSALQLAKRPENAGKLIVVVIPSFGERY
HHHCCCCHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHH
LSTVLFEHLG
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]