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The map label for this gene is rlmN

Identifier: 34498996

GI number: 34498996

Start: 3849442

End: 3850536

Strand: Reverse

Name: rlmN

Synonym: CV_3541

Alternate gene names: 34498996

Gene position: 3850536-3849442 (Counterclockwise)

Preceding gene: 34498997

Following gene: 34498995

Centisome position: 81.05

GC content: 62.56

Gene sequence:

>1095_bases
ATGAAAACCAACCTGCTCGACTTCAATCTGGACCAGCTGACGCAGCACTTCGCGGAGATGGGCGAGAAGCCGTTCCGCGC
CAAGCAAGTGATGCGTTGGATGCACCAGATGGCCGAAGACGATTTCGACGCGATGACCGACCTCGCCAAGAGCCTGCGCG
CCAAGCTGCACGAGCGCGCCGAAGTGCGCGTGCCGTCGCTGATGACCGGGCAGGCGTCCAGTGATGGCACCCGCAAGTGG
CTGCTCGATGTCGGCACCGGCAACGGTGTGGAAACCGTCTTCATTCCGGAAGACGATCGCGGCACCTTGTGCGTGTCATC
CCAGGTGGGCTGCGCGCTGGAGTGCACCTTCTGCTCCACCGGCCGCCAGGGATTCAACCGCAACCTGTCCACCGCCGAGA
TCATCGGCCAGCTGTGGTGGGCGAACAAGGCGATGGGCGTGACGCCCAAGAACGAGCGCGTGGTGTCGAACGTGGTGATG
ATGGGCATGGGCGAGCCCCTGGCCAACTTCGACAACGTGGTCAGCGCGATGCAGATCATGCTGGACGACCACGGCTACGG
CCTGTCCCGCCGCCGCGTGACGCTGTCCACCTCCGGCCTGGTGCCGCAAATGGACCGGTTGCGCGAGGAGTGCCCGGTGG
CGCTGGCGGTGTCGCTGCACGCGCCGAACGACGCGATCCGCGACGTGATCGTGCCGATCAACAAGAAATATCCGCTGTCG
GAGCTGATGGCGGCCTGTCGCCGCTACCTGGAAAAGGCACCGCGCGATTTCATCACCTTTGAATATGTGATGCTTGACGG
GGTCAACGATAGGCCGGAACATGCTCGCCAGCTGCTGGAGCTGGTGCGGGACGTGCCCTGCAAGTTCAACCTGATCCCGT
TCAATCCTTTCCCCAACTCGGGTTATGATCGTTCCAGCAACAACGCGATTCGCATTTTCCGTGAAATTCTGCAAGAACAA
GGTTACGTGGTGACCGTGCGCAAGACCCGCGGCGACGATATCGACGCGGCCTGCGGCCAGCTGGCCGGCCAGGTGCAGGA
CAAGACGCGGCGCCAGGCCAAGTGGACGCAGATCATAGAGGACAGGAAATCATGA

Upstream 100 bases:

>100_bases
TTTCTTCGCCGCTTCCGAAATCAGCTCCCGCTGATTCAAAAGTAGTCGAATGACCGGAGGTCGCCGCTGCCGGCGGCCTC
TTTTGCCTGTTGAGGATTGC

Downstream 100 bases:

>100_bases
CGTCATGGCGGGGGAGATTGGCTGGATGGCTGTTGGCATTTGGCGTAATCGCCCCCGCCGCGGCGGATGGCAGCAATCCG
TCGGAAGTGGCGAGGATCCG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MKTNLLDFNLDQLTQHFAEMGEKPFRAKQVMRWMHQMAEDDFDAMTDLAKSLRAKLHERAEVRVPSLMTGQASSDGTRKW
LLDVGTGNGVETVFIPEDDRGTLCVSSQVGCALECTFCSTGRQGFNRNLSTAEIIGQLWWANKAMGVTPKNERVVSNVVM
MGMGEPLANFDNVVSAMQIMLDDHGYGLSRRRVTLSTSGLVPQMDRLREECPVALAVSLHAPNDAIRDVIVPINKKYPLS
ELMAACRRYLEKAPRDFITFEYVMLDGVNDRPEHARQLLELVRDVPCKFNLIPFNPFPNSGYDRSSNNAIRIFREILQEQ
GYVVTVRKTRGDDIDAACGQLAGQVQDKTRRQAKWTQIIEDRKS

Sequences:

>Translated_364_residues
MKTNLLDFNLDQLTQHFAEMGEKPFRAKQVMRWMHQMAEDDFDAMTDLAKSLRAKLHERAEVRVPSLMTGQASSDGTRKW
LLDVGTGNGVETVFIPEDDRGTLCVSSQVGCALECTFCSTGRQGFNRNLSTAEIIGQLWWANKAMGVTPKNERVVSNVVM
MGMGEPLANFDNVVSAMQIMLDDHGYGLSRRRVTLSTSGLVPQMDRLREECPVALAVSLHAPNDAIRDVIVPINKKYPLS
ELMAACRRYLEKAPRDFITFEYVMLDGVNDRPEHARQLLELVRDVPCKFNLIPFNPFPNSGYDRSSNNAIRIFREILQEQ
GYVVTVRKTRGDDIDAACGQLAGQVQDKTRRQAKWTQIIEDRKS
>Mature_364_residues
MKTNLLDFNLDQLTQHFAEMGEKPFRAKQVMRWMHQMAEDDFDAMTDLAKSLRAKLHERAEVRVPSLMTGQASSDGTRKW
LLDVGTGNGVETVFIPEDDRGTLCVSSQVGCALECTFCSTGRQGFNRNLSTAEIIGQLWWANKAMGVTPKNERVVSNVVM
MGMGEPLANFDNVVSAMQIMLDDHGYGLSRRRVTLSTSGLVPQMDRLREECPVALAVSLHAPNDAIRDVIVPINKKYPLS
ELMAACRRYLEKAPRDFITFEYVMLDGVNDRPEHARQLLELVRDVPCKFNLIPFNPFPNSGYDRSSNNAIRIFREILQEQ
GYVVTVRKTRGDDIDAACGQLAGQVQDKTRRQAKWTQIIEDRKS

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=353, Percent_Identity=62.6062322946176, Blast_Score=448, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_CHRVO (Q7NS85)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903211.1
- ProteinModelPortal:   Q7NS85
- GeneID:   2547457
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3541
- NMPDR:   fig|243365.1.peg.3541
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   Q7NS85
- ProtClustDB:   CLSK877994
- BioCyc:   CVIO243365:CV_3541-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 41091; Mature: 41091

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNLLDFNLDQLTQHFAEMGEKPFRAKQVMRWMHQMAEDDFDAMTDLAKSLRAKLHERA
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
EVRVPSLMTGQASSDGTRKWLLDVGTGNGVETVFIPEDDRGTLCVSSQVGCALECTFCST
HCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEECCCCCEEEEEECCC
GRQGFNRNLSTAEIIGQLWWANKAMGVTPKNERVVSNVVMMGMGEPLANFDNVVSAMQIM
CCHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LDDHGYGLSRRRVTLSTSGLVPQMDRLREECPVALAVSLHAPNDAIRDVIVPINKKYPLS
HHCCCCCCCCCEEEEECCCCCHHHHHHHHHCCEEEEEEEECCCHHHHHHEECCCCCCCHH
ELMAACRRYLEKAPRDFITFEYVMLDGVNDRPEHARQLLELVRDVPCKFNLIPFNPFPNS
HHHHHHHHHHHHCCCHHEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCC
GYDRSSNNAIRIFREILQEQGYVVTVRKTRGDDIDAACGQLAGQVQDKTRRQAKWTQIIE
CCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRKS
HCCC
>Mature Secondary Structure
MKTNLLDFNLDQLTQHFAEMGEKPFRAKQVMRWMHQMAEDDFDAMTDLAKSLRAKLHERA
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
EVRVPSLMTGQASSDGTRKWLLDVGTGNGVETVFIPEDDRGTLCVSSQVGCALECTFCST
HCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEECCCCCEEEEEECCC
GRQGFNRNLSTAEIIGQLWWANKAMGVTPKNERVVSNVVMMGMGEPLANFDNVVSAMQIM
CCHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LDDHGYGLSRRRVTLSTSGLVPQMDRLREECPVALAVSLHAPNDAIRDVIVPINKKYPLS
HHCCCCCCCCCEEEEECCCCCHHHHHHHHHCCEEEEEEEECCCHHHHHHEECCCCCCCHH
ELMAACRRYLEKAPRDFITFEYVMLDGVNDRPEHARQLLELVRDVPCKFNLIPFNPFPNS
HHHHHHHHHHHHCCCHHEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCC
GYDRSSNNAIRIFREILQEQGYVVTVRKTRGDDIDAACGQLAGQVQDKTRRQAKWTQIIE
CCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRKS
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782