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The map label for this gene is afsQ1 [H]

Identifier: 34498960

GI number: 34498960

Start: 3804354

End: 3805562

Strand: Reverse

Name: afsQ1 [H]

Synonym: CV_3505

Alternate gene names: 34498960

Gene position: 3805562-3804354 (Counterclockwise)

Preceding gene: 34498961

Following gene: 34498953

Centisome position: 80.1

GC content: 65.59

Gene sequence:

>1209_bases
ATGTTCCGGGTTTGGGGCAACCCGCAATGGCTTGGGGATCCGATGTCGTCGACGACCATCTTGTTGGTAGAGGATAGCGC
CACTAACCGCTATTTCATCGAGCAATTCATCCAGGAGCTCGGCTATCGCTGCGCGTCGGCGACGAACGGCGTGGAGGCGG
TGGAGTATTGCCGGAACCAGGTGCCGGACTTGGTGCTGATGGACATCATCATGCCGGAGATGGACGGCTTGCAAGCCACC
ACCGAACTGCGCAGGCTGTTCGGCGAGCATTGGGTGCCCATCATCTTTTTGACCAGCCTGAACGAGCTGGAGAGCGTGGT
GTCGGGCCTAAAGGCCGGCGGCGACGACTACCTGGCCAAGCCTGTCAGCTTCGACCTGCTGTCCGCCAAGATCCATGTCT
TCCTGCGCATCGCGCAGATGCAGAACCAGATCAACCAGGATGCGATCCGGCTGGAGAAGTATTACCAGGAAAACGAGTTC
GAACAGCAGCTGGCGCTGGAGCTGATCGAGCGGCTGGTCCGGCTGCGCACCTCGCGGCCGGGCCATGTCTGGCAATATCT
GAATCCCGCAGCAGGCTTCAATGGAGACCTGATCATCATCTGCCAGCCGCCGGGAGGCGGCGAGCACATCATGCTGGCCG
ATTGCACCGGCCATGGGTTGACGGCCGCGATCAGCGCGCTGCCGGCGATAGACTGCTTTCACGAAATGACGGAGGCCGGC
TGCGACATGGCCGCCATCGCGCGCGGGGTCAACCAGAAACTGCACAAGCTATTGCCGGGCGGCCGATTCGTCGCCGCGGC
GCTGATCGCGATCGACGCCGACGGGCGCCATGCGCGGGTCTGGAACGGCGGCGTGCCCTGCGTGCTGCTGTTCAACGAGA
AGGGCGAGGTCGAGGCGCGCTGCAAGTCGGCTCACCCGCCGTTGGGCGTGCTGCCGGCCGAAGCTTTCGACGACAGCATG
CAGACCCTGGCCTGGCTGCCGGAGCAGGCGCTGGTGGTCAGTTCGGACGGCATCACCGAGGCCAAGGACAGCCAGGGGGC
GATGTTCGGCCTGGTCGGCGTGGAGGCGGCGGTCCGTCGCGGCTGGCCGGACCGGATCGGGGAGGCGGTGCTGGCGGCGG
CCAAGGCGCACATGCAGGGCGGCGCGGCCAGCGATGACGTCTCGCTGCTGGTGGTCCGCCATCCTGGGGGCGGCGCCTCG
GCTGGGTGA

Upstream 100 bases:

>100_bases
CGGGTAATGAGGTGGAATACCGACCGGGCGCGTCTTATTATGGAATGGCCCGAAGCATGGGATGGGAGGGCCGAGGCCGC
AGAGGAGGCTGGCGGCCCTG

Downstream 100 bases:

>100_bases
GGCTGGTGTTGGAACCTGTCCAAAGTCTTTTTGCGGCAGCGGTCATTCTATGCCGGATGCCAGGCGCGGAAAACGGCACG
CCGCAGTGCCATTCACTGCA

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MFRVWGNPQWLGDPMSSTTILLVEDSATNRYFIEQFIQELGYRCASATNGVEAVEYCRNQVPDLVLMDIIMPEMDGLQAT
TELRRLFGEHWVPIIFLTSLNELESVVSGLKAGGDDYLAKPVSFDLLSAKIHVFLRIAQMQNQINQDAIRLEKYYQENEF
EQQLALELIERLVRLRTSRPGHVWQYLNPAAGFNGDLIIICQPPGGGEHIMLADCTGHGLTAAISALPAIDCFHEMTEAG
CDMAAIARGVNQKLHKLLPGGRFVAAALIAIDADGRHARVWNGGVPCVLLFNEKGEVEARCKSAHPPLGVLPAEAFDDSM
QTLAWLPEQALVVSSDGITEAKDSQGAMFGLVGVEAAVRRGWPDRIGEAVLAAAKAHMQGGAASDDVSLLVVRHPGGGAS
AG

Sequences:

>Translated_402_residues
MFRVWGNPQWLGDPMSSTTILLVEDSATNRYFIEQFIQELGYRCASATNGVEAVEYCRNQVPDLVLMDIIMPEMDGLQAT
TELRRLFGEHWVPIIFLTSLNELESVVSGLKAGGDDYLAKPVSFDLLSAKIHVFLRIAQMQNQINQDAIRLEKYYQENEF
EQQLALELIERLVRLRTSRPGHVWQYLNPAAGFNGDLIIICQPPGGGEHIMLADCTGHGLTAAISALPAIDCFHEMTEAG
CDMAAIARGVNQKLHKLLPGGRFVAAALIAIDADGRHARVWNGGVPCVLLFNEKGEVEARCKSAHPPLGVLPAEAFDDSM
QTLAWLPEQALVVSSDGITEAKDSQGAMFGLVGVEAAVRRGWPDRIGEAVLAAAKAHMQGGAASDDVSLLVVRHPGGGAS
AG
>Mature_402_residues
MFRVWGNPQWLGDPMSSTTILLVEDSATNRYFIEQFIQELGYRCASATNGVEAVEYCRNQVPDLVLMDIIMPEMDGLQAT
TELRRLFGEHWVPIIFLTSLNELESVVSGLKAGGDDYLAKPVSFDLLSAKIHVFLRIAQMQNQINQDAIRLEKYYQENEF
EQQLALELIERLVRLRTSRPGHVWQYLNPAAGFNGDLIIICQPPGGGEHIMLADCTGHGLTAAISALPAIDCFHEMTEAG
CDMAAIARGVNQKLHKLLPGGRFVAAALIAIDADGRHARVWNGGVPCVLLFNEKGEVEARCKSAHPPLGVLPAEAFDDSM
QTLAWLPEQALVVSSDGITEAKDSQGAMFGLVGVEAAVRRGWPDRIGEAVLAAAKAHMQGGAASDDVSLLVVRHPGGGAS
AG

Specific function: Forms part of a two-component regulatory system AfsQ1/AfsQ2 involved in secondary metabolism [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=112, Percent_Identity=37.5, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788550, Length=127, Percent_Identity=34.6456692913386, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788394, Length=182, Percent_Identity=27.4725274725275, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1790299, Length=129, Percent_Identity=30.2325581395349, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082012, Length=117, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786784, Length=117, Percent_Identity=32.4786324786325, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790860, Length=180, Percent_Identity=30, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786599, Length=117, Percent_Identity=34.1880341880342, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI145693157, Length=115, Percent_Identity=33.0434782608696, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786911, Length=121, Percent_Identity=32.2314049586777, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787375, Length=117, Percent_Identity=30.7692307692308, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI48994928, Length=121, Percent_Identity=33.8842975206612, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787487, Length=124, Percent_Identity=29.8387096774194, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790552, Length=117, Percent_Identity=32.4786324786325, Blast_Score=62, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322000, Length=117, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 43596; Mature: 43596

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRVWGNPQWLGDPMSSTTILLVEDSATNRYFIEQFIQELGYRCASATNGVEAVEYCRNQ
CEEECCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
VPDLVLMDIIMPEMDGLQATTELRRLFGEHWVPIIFLTSLNELESVVSGLKAGGDDYLAK
CCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHCCCCHHHCC
PVSFDLLSAKIHVFLRIAQMQNQINQDAIRLEKYYQENEFEQQLALELIERLVRLRTSRP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GHVWQYLNPAAGFNGDLIIICQPPGGGEHIMLADCTGHGLTAAISALPAIDCFHEMTEAG
CCHHHHCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
CDMAAIARGVNQKLHKLLPGGRFVAAALIAIDADGRHARVWNGGVPCVLLFNEKGEVEAR
CCHHHHHHHHHHHHHHHCCCCHHEEEEEEEECCCCCEEEEECCCCCEEEEECCCCCHHHH
CKSAHPPLGVLPAEAFDDSMQTLAWLPEQALVVSSDGITEAKDSQGAMFGLVGVEAAVRR
HHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHHC
GWPDRIGEAVLAAAKAHMQGGAASDDVSLLVVRHPGGGASAG
CCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MFRVWGNPQWLGDPMSSTTILLVEDSATNRYFIEQFIQELGYRCASATNGVEAVEYCRNQ
CEEECCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
VPDLVLMDIIMPEMDGLQATTELRRLFGEHWVPIIFLTSLNELESVVSGLKAGGDDYLAK
CCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHCCCCHHHCC
PVSFDLLSAKIHVFLRIAQMQNQINQDAIRLEKYYQENEFEQQLALELIERLVRLRTSRP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GHVWQYLNPAAGFNGDLIIICQPPGGGEHIMLADCTGHGLTAAISALPAIDCFHEMTEAG
CCHHHHCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
CDMAAIARGVNQKLHKLLPGGRFVAAALIAIDADGRHARVWNGGVPCVLLFNEKGEVEAR
CCHHHHHHHHHHHHHHHCCCCHHEEEEEEEECCCCCEEEEECCCCCEEEEECCCCCHHHH
CKSAHPPLGVLPAEAFDDSMQTLAWLPEQALVVSSDGITEAKDSQGAMFGLVGVEAAVRR
HHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHHC
GWPDRIGEAVLAAAKAHMQGGAASDDVSLLVVRHPGGGASAG
CCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339426; 12000953 [H]