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The map label for this gene is nfsA [H]

Identifier: 34498955

GI number: 34498955

Start: 3794115

End: 3794855

Strand: Direct

Name: nfsA [H]

Synonym: CV_3500

Alternate gene names: 34498955

Gene position: 3794115-3794855 (Clockwise)

Preceding gene: 34498954

Following gene: 34498956

Centisome position: 79.86

GC content: 66.67

Gene sequence:

>741_bases
ATGAACGAAACCTTGCAGTTGATGCATTCCCATCGCAGCGTGCGCAGCTACCAGGACAAACCTGTCGCGCCGGAACTGCT
GGACGCCGTGCTGGACGCCGCCTGGAAAGGCCCCACCTCGATCAACGGCCAGCAGATCTCGCTGGTGGTGGTGCAGGACC
CGGAACGCCGCAAGCGCATCGCCGAAATCGCCGGCGGCCAGCCGTGGATCGCGCAGGCGCCGGTCTTCGTCGCCGTGGTG
ATGGATTTCCACAAGACCCGCCTGGGCGTGGAAATGGCCGGCGAGAAACAGGTGATACACGACAGCGTCGAAGCCTTCGC
CGTCGGATCGGTGGATGCCGGCATCGCGCTGGGCAATATGATCACCGCCGCGCACGCCGCCGGCCTGGGCGTGGTGCCGA
TCGGCGGCATCCGCCGCGATCCGCAGGCGATGATAGACCTGCTGGAACTGCCGGAACTGACCTTTCCGCTGGTTGGCCTG
GTGATCGGCCACGTTCAGGATGACGCCGATCTCAAGCCCCGCCTGCCGCGCGCGAGCTTCATCCACCGCGAAAGCTATCA
GGCCGCGGAGCTGGCCGGCCACATCGCCGCCTACGACGACACGCTGCGCCGCCACTGGCAGGACATCGGCCGCGCCGACG
GCGAACCATGGACCGCCAACACCGCCCGCTACTACCGCCAGGTTTATTTCCCCAAGGTCGCGCCCGTCGCGCGCGCCCAG
GGTTTTGGCTTCGACCAGTAA

Upstream 100 bases:

>100_bases
GATTTGGCCAGCTGCAAGCGATAAAGCACTCACCCGCCGAGCCGGCGGGTTTTCTTGACATCAAAACTAGACGACTGGTC
TACTCAAAACGGAGCCACAC

Downstream 100 bases:

>100_bases
CTCCGACCACGCCTTCAAGGAAACCGACATGAACGCCGACAAACTGCTGACCCCGCTGACCATGGGCGCCGTCGCCCTGA
GCAACCGCGTCGTCATGGCC

Product: oxygen-insensitive NADPH nitroreductase

Products: NA

Alternate protein names: NAD(P)H-dependent FMN reductase; NAD(P)H-dependent nitroreductase; NAD(P)H-dependent oxidoreductase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MNETLQLMHSHRSVRSYQDKPVAPELLDAVLDAAWKGPTSINGQQISLVVVQDPERRKRIAEIAGGQPWIAQAPVFVAVV
MDFHKTRLGVEMAGEKQVIHDSVEAFAVGSVDAGIALGNMITAAHAAGLGVVPIGGIRRDPQAMIDLLELPELTFPLVGL
VIGHVQDDADLKPRLPRASFIHRESYQAAELAGHIAAYDDTLRRHWQDIGRADGEPWTANTARYYRQVYFPKVAPVARAQ
GFGFDQ

Sequences:

>Translated_246_residues
MNETLQLMHSHRSVRSYQDKPVAPELLDAVLDAAWKGPTSINGQQISLVVVQDPERRKRIAEIAGGQPWIAQAPVFVAVV
MDFHKTRLGVEMAGEKQVIHDSVEAFAVGSVDAGIALGNMITAAHAAGLGVVPIGGIRRDPQAMIDLLELPELTFPLVGL
VIGHVQDDADLKPRLPRASFIHRESYQAAELAGHIAAYDDTLRRHWQDIGRADGEPWTANTARYYRQVYFPKVAPVARAQ
GFGFDQ
>Mature_246_residues
MNETLQLMHSHRSVRSYQDKPVAPELLDAVLDAAWKGPTSINGQQISLVVVQDPERRKRIAEIAGGQPWIAQAPVFVAVV
MDFHKTRLGVEMAGEKQVIHDSVEAFAVGSVDAGIALGNMITAAHAAGLGVVPIGGIRRDPQAMIDLLELPELTFPLVGL
VIGHVQDDADLKPRLPRASFIHRESYQAAELAGHIAAYDDTLRRHWQDIGRADGEPWTANTARYYRQVYFPKVAPVARAQ
GFGFDQ

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones, chromates and azo dyes. It could supply the reduced form of FMN to luciferase-like protein and contribute to the degradation of aromatic compounds [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=244, Percent_Identity=30.327868852459, Blast_Score=117, Evalue=9e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 26970; Mature: 26970

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNETLQLMHSHRSVRSYQDKPVAPELLDAVLDAAWKGPTSINGQQISLVVVQDPERRKRI
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHHHH
AEIAGGQPWIAQAPVFVAVVMDFHKTRLGVEMAGEKQVIHDSVEAFAVGSVDAGIALGNM
HHHCCCCCCHHHCHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHEECCCHHHHHHHHH
ITAAHAAGLGVVPIGGIRRDPQAMIDLLELPELTFPLVGLVIGHVQDDADLKPRLPRASF
HHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHH
IHRESYQAAELAGHIAAYDDTLRRHWQDIGRADGEPWTANTARYYRQVYFPKVAPVARAQ
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHC
GFGFDQ
CCCCCC
>Mature Secondary Structure
MNETLQLMHSHRSVRSYQDKPVAPELLDAVLDAAWKGPTSINGQQISLVVVQDPERRKRI
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHHHH
AEIAGGQPWIAQAPVFVAVVMDFHKTRLGVEMAGEKQVIHDSVEAFAVGSVDAGIALGNM
HHHCCCCCCHHHCHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHEECCCHHHHHHHHH
ITAAHAAGLGVVPIGGIRRDPQAMIDLLELPELTFPLVGLVIGHVQDDADLKPRLPRASF
HHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHH
IHRESYQAAELAGHIAAYDDTLRRHWQDIGRADGEPWTANTARYYRQVYFPKVAPVARAQ
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHC
GFGFDQ
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]