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The map label for this gene is phrB [H]

Identifier: 34498936

GI number: 34498936

Start: 3776451

End: 3777866

Strand: Reverse

Name: phrB [H]

Synonym: CV_3481

Alternate gene names: 34498936

Gene position: 3777866-3776451 (Counterclockwise)

Preceding gene: 34498937

Following gene: 34498934

Centisome position: 79.52

GC content: 67.02

Gene sequence:

>1416_bases
ATGAGCAAACGTTATCGGACGGCCTTGGTCTGGCTGCGCCGCGACCTGCGGCTGGACGACCACGCCGCTTTTTACCATGC
GCTGAAGCACAGCGAGGCCGTGCTGCCGGTGTTCGTGTTCGATGCCTCGATACTCGCGGCCTTGCCGCGCGACGACCGGC
GCGTGGATTTCATCTGGCAGAGCGCGGCGGCGCTGAAGGCCGAGCTGAACCAGCTGGGCGGCGACCTGCTGATCCGCCAT
GGCCTGCCGCAACACGAGATACCGCGGCTGGCGGCGGAATTGGGCGCCGAGGCGGTATTCTGCAACCGCGACTACGAGCC
GGCCGCCCGCGCGCGCGACGCCGAGGTGGCGCAACGGCTGGCCGAACAGGGCGTCGCCTTCCGCGACAGCAAAGACCAGG
TGATTTTCGAGACCGACGAAGTCTTGACCGGGGCCGGCAAACCGTTCTCGGTGTTCACGCCGTACAAGAACGCCTGGTTG
AAGAAACTGACGCCGTTCCACCTGCAGGCCTACCCGGTGCGGCGCTATCTGGACGCGCTGATGCCGCGTGAACCGCAATC
CATGCCCAGCCTGGCCGATATCGGTTTCCTGCCCAGCGATCTGGCCTCGCTGCCGGTCAAGGCCGGCGCGGCCGGGGCCG
AGGCGCTGTTCGCCGACTTCGCCGGCCGCATCGAGCGCTACAAGGCGCAGCGCGACTTTCCCGGCGTCAAGGGCGTGTCC
TATCTCAGCGTCCACCTGCGCTTCGGCACTCTCTCCATCCGCCGGCTGGCCGCCTACGCCTGGCATGACGGCGGCGAAGG
CGCGATGACCTGGCTGAGCGAGCTGATCTGGCGCGACTTCTACCAGCAGGTGCTGTGGCACCGGCCCGATGCGGCGAAGC
ACGCGTTCAAGCCGGAGTACGACGCGTTGCCGTTTCCCAACGATCCGGAATGGTTCGACGCCTGGCGCGAAGGCCGCACC
GGCTACCCGCTGGTGGACGCGGCGATGCGTCAGCTCAATCGCAGCGGCTACATGCACAACCGCTTGCGCATGGTGGCGGC
CAGCTTCCTGGTCAAGGATCTGCTGATCGACTGGCGCTGGGGGGAGCAGTACTTTGCCGAGAAACTGCTGGACTTCGACC
TGGCGGCCAACAACGGCGGCTGGCAGTGGGCGGCCAGCACCGGTTGCGACGCCCAGCCTTATTTCCGCATCTTCAATCCG
GTCAGCCAGAGCGAGAAGTTCGACCCCGACGGCCGCTTCATCCGCCGTTACGTGCCGGAGCTGGCGGCGCTGCCGGACAA
GCTGATCCACGCGCCGTGGCAGGCCAAGCCGGAACAACTGATGTCAGCCGGCGTGCGGCTGGGACGCGACTATCCGCATC
CCATCGTCGACCACGCGGTTCAGCGCGAGCTGGCGCTGGCGCTGTTCAAGCGCTGA

Upstream 100 bases:

>100_bases
CCTTCATCGACTTCCTTGACGAAAAACTGGGCGGCGACAGGCCGTTGTGGGATAGCGACCTGCCCCCATCTGTGCTGTCA
TCTCAATATGGGGAGCGCCC

Downstream 100 bases:

>100_bases
ATTCGTCGGGCCAGCCGCCGCTTTCCAGCAGTTGGCGGCGGTAGCGTTCCGGCGAGTCGCCCAGATGTTTCTGGAACATG
GCGATGAAGGCCGATGGCGT

Product: deoxyribodipyrimidine photo-lyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSKRYRTALVWLRRDLRLDDHAAFYHALKHSEAVLPVFVFDASILAALPRDDRRVDFIWQSAAALKAELNQLGGDLLIRH
GLPQHEIPRLAAELGAEAVFCNRDYEPAARARDAEVAQRLAEQGVAFRDSKDQVIFETDEVLTGAGKPFSVFTPYKNAWL
KKLTPFHLQAYPVRRYLDALMPREPQSMPSLADIGFLPSDLASLPVKAGAAGAEALFADFAGRIERYKAQRDFPGVKGVS
YLSVHLRFGTLSIRRLAAYAWHDGGEGAMTWLSELIWRDFYQQVLWHRPDAAKHAFKPEYDALPFPNDPEWFDAWREGRT
GYPLVDAAMRQLNRSGYMHNRLRMVAASFLVKDLLIDWRWGEQYFAEKLLDFDLAANNGGWQWAASTGCDAQPYFRIFNP
VSQSEKFDPDGRFIRRYVPELAALPDKLIHAPWQAKPEQLMSAGVRLGRDYPHPIVDHAVQRELALALFKR

Sequences:

>Translated_471_residues
MSKRYRTALVWLRRDLRLDDHAAFYHALKHSEAVLPVFVFDASILAALPRDDRRVDFIWQSAAALKAELNQLGGDLLIRH
GLPQHEIPRLAAELGAEAVFCNRDYEPAARARDAEVAQRLAEQGVAFRDSKDQVIFETDEVLTGAGKPFSVFTPYKNAWL
KKLTPFHLQAYPVRRYLDALMPREPQSMPSLADIGFLPSDLASLPVKAGAAGAEALFADFAGRIERYKAQRDFPGVKGVS
YLSVHLRFGTLSIRRLAAYAWHDGGEGAMTWLSELIWRDFYQQVLWHRPDAAKHAFKPEYDALPFPNDPEWFDAWREGRT
GYPLVDAAMRQLNRSGYMHNRLRMVAASFLVKDLLIDWRWGEQYFAEKLLDFDLAANNGGWQWAASTGCDAQPYFRIFNP
VSQSEKFDPDGRFIRRYVPELAALPDKLIHAPWQAKPEQLMSAGVRLGRDYPHPIVDHAVQRELALALFKR
>Mature_470_residues
SKRYRTALVWLRRDLRLDDHAAFYHALKHSEAVLPVFVFDASILAALPRDDRRVDFIWQSAAALKAELNQLGGDLLIRHG
LPQHEIPRLAAELGAEAVFCNRDYEPAARARDAEVAQRLAEQGVAFRDSKDQVIFETDEVLTGAGKPFSVFTPYKNAWLK
KLTPFHLQAYPVRRYLDALMPREPQSMPSLADIGFLPSDLASLPVKAGAAGAEALFADFAGRIERYKAQRDFPGVKGVSY
LSVHLRFGTLSIRRLAAYAWHDGGEGAMTWLSELIWRDFYQQVLWHRPDAAKHAFKPEYDALPFPNDPEWFDAWREGRTG
YPLVDAAMRQLNRSGYMHNRLRMVAASFLVKDLLIDWRWGEQYFAEKLLDFDLAANNGGWQWAASTGCDAQPYFRIFNPV
SQSEKFDPDGRFIRRYVPELAALPDKLIHAPWQAKPEQLMSAGVRLGRDYPHPIVDHAVQRELALALFKR

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=489, Percent_Identity=28.4253578732106, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI4758072, Length=496, Percent_Identity=28.2258064516129, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI188536103, Length=439, Percent_Identity=28.7015945330296, Blast_Score=178, Evalue=9e-45,
Organism=Escherichia coli, GI1786926, Length=480, Percent_Identity=44.1666666666667, Blast_Score=328, Evalue=4e-91,
Organism=Saccharomyces cerevisiae, GI6324962, Length=510, Percent_Identity=29.6078431372549, Blast_Score=200, Evalue=4e-52,
Organism=Drosophila melanogaster, GI17137248, Length=492, Percent_Identity=29.0650406504065, Blast_Score=192, Evalue=5e-49,
Organism=Drosophila melanogaster, GI24585455, Length=492, Percent_Identity=29.0650406504065, Blast_Score=192, Evalue=5e-49,
Organism=Drosophila melanogaster, GI24648152, Length=508, Percent_Identity=26.9685039370079, Blast_Score=163, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 53691; Mature: 53559

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRYRTALVWLRRDLRLDDHAAFYHALKHSEAVLPVFVFDASILAALPRDDRRVDFIWQ
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCHHHHHH
SAAALKAELNQLGGDLLIRHGLPQHEIPRLAAELGAEAVFCNRDYEPAARARDAEVAQRL
HHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCEEEECCCCCHHHHCCHHHHHHHH
AEQGVAFRDSKDQVIFETDEVLTGAGKPFSVFTPYKNAWLKKLTPFHLQAYPVRRYLDAL
HHCCCEECCCCCCEEEECHHHHHCCCCCCEEECCHHHHHHHHCCCHHHHHHHHHHHHHHH
MPREPQSMPSLADIGFLPSDLASLPVKAGAAGAEALFADFAGRIERYKAQRDFPGVKGVS
CCCCCCCCCCHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
YLSVHLRFGTLSIRRLAAYAWHDGGEGAMTWLSELIWRDFYQQVLWHRPDAAKHAFKPEY
EEEEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCC
DALPFPNDPEWFDAWREGRTGYPLVDAAMRQLNRSGYMHNRLRMVAASFLVKDLLIDWRW
CCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
GEQYFAEKLLDFDLAANNGGWQWAASTGCDAQPYFRIFNPVSQSEKFDPDGRFIRRYVPE
CHHHHHHHHHHHHEECCCCCEEEECCCCCCCCCHHHHHCCHHCCCCCCCCHHHHHHHHHH
LAALPDKLIHAPWQAKPEQLMSAGVRLGRDYPHPIVDHAVQRELALALFKR
HHHCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKRYRTALVWLRRDLRLDDHAAFYHALKHSEAVLPVFVFDASILAALPRDDRRVDFIWQ
CCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCHHHHHH
SAAALKAELNQLGGDLLIRHGLPQHEIPRLAAELGAEAVFCNRDYEPAARARDAEVAQRL
HHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCEEEECCCCCHHHHCCHHHHHHHH
AEQGVAFRDSKDQVIFETDEVLTGAGKPFSVFTPYKNAWLKKLTPFHLQAYPVRRYLDAL
HHCCCEECCCCCCEEEECHHHHHCCCCCCEEECCHHHHHHHHCCCHHHHHHHHHHHHHHH
MPREPQSMPSLADIGFLPSDLASLPVKAGAAGAEALFADFAGRIERYKAQRDFPGVKGVS
CCCCCCCCCCHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
YLSVHLRFGTLSIRRLAAYAWHDGGEGAMTWLSELIWRDFYQQVLWHRPDAAKHAFKPEY
EEEEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCC
DALPFPNDPEWFDAWREGRTGYPLVDAAMRQLNRSGYMHNRLRMVAASFLVKDLLIDWRW
CCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
GEQYFAEKLLDFDLAANNGGWQWAASTGCDAQPYFRIFNPVSQSEKFDPDGRFIRRYVPE
CHHHHHHHHHHHHEECCCCCEEEECCCCCCCCCHHHHHCCHHCCCCCCCCHHHHHHHHHH
LAALPDKLIHAPWQAKPEQLMSAGVRLGRDYPHPIVDHAVQRELALALFKR
HHHCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]