Click here to switch to the map view.

The map label for this gene is proP [H]

Identifier: 34498921

GI number: 34498921

Start: 3761373

End: 3762683

Strand: Direct

Name: proP [H]

Synonym: CV_3466

Alternate gene names: 34498921

Gene position: 3761373-3762683 (Clockwise)

Preceding gene: 34498920

Following gene: 34498922

Centisome position: 79.17

GC content: 69.03

Gene sequence:

>1311_bases
TTGACCATGACTGACTCCCGATCCACCCCGCTCACCCGCTCGGACTACCAGACCCTGGGCCTGGCCGCGCTGGGCGGCGC
GCTGGAAATCTACGATTTCATCATCTTCGCCTTCTTCGCCACCACGCTGAGCAAGCTGTTCTTCCCGCCCGACATGCCGG
AGTGGCTGCGCCTGCTGCAGACCTTCGGCATCTTCGCCACCGGCTACCTGGCGCGGCCGCTGGGCGGCATCCTGATGGCG
CACTACGCCGACCGGCTGGGCCGCAAGCGCGTGTTCAGCCTGACCATCCTGATGATGGCCGGCCCCTGCCTGCTGATCGG
CCTGATGCCCACCTACGCCGACATCGGCATCCTGGCGCCGCTGCTGCTGCTGTCGCTGCGCATTCTGCAGGGCGCGGCCG
TGGGCGGCGAAGTGCCCAGCGCCTGGTCCTTCGTCGCCGAGCATGCGCCGCGCGGCCGACGCGGCTACGCGCTGGGCGTG
CTGCAGGCCGGGCTGACCTTCGGCTACCTGCTGGGCGCCCTGACCGCCACGCTGCTGGCGCGCGTGTTCACGCCGCAGGA
GATGCTGGACTACGGCTGGCGCATCCCCTTTCTGCTGGGCGGGCCCTTCGGCCTGATTGGGGTCTGGCTGCGGCGCTGGC
TGAGCGAGACGCCGGTGTTCCTGGCGCTGCGCGAGCGCCGGGACGCGCGCGCCGCCTACCCCTTGCTGGCCGTGCTGCGC
CGGCACAAGCACACGCTGCTGCCGGCCGCTGTGCTGACCTGCGTGCTCACCTCCGCGGTGGTGGTGCTGGTGGTGGTGAC
CCCCACCCTGCTGCAGCAACGCTTCGGCATGCACGCCGACCGCGCCTTCGCGCTCAGCAGCGTGGGCATCGTGTTTCTGA
ACGTCGGCTGCGTAATCGCCGGCCACGTTTGCGATCGCATCGGCCAGCGCCGCAGCCTGGTCCTGTACAGCCTGCTGCTG
CCGATCGGCATCGCCGCGCTGTACGCCGACCTGATCGCCGGCTGGGGCTGGCCCGGCCTGGCCTACGCCTTCGCCGGCCT
GTGCTGCGGCATCGTCGGCGTGGTGCCATCCATCATGGTCGGCATGTTCCCGGCCGAGGTGCGGGTGTCGGGCATTTCCT
TCACCTACAACGTTGCTTACTCGGCCTGGGCCAGCGTGACCCCGTTGCTGCTGATCGCCGCCATGCCATGGAGTCCGTGG
ATCTGCGTGCTCTATTGCGCAACCATGGCGCTGACCGGCTTGTTCTGCCTGCGTTCGGCCCGAAACACGCAGGAACCTTC
GCTGGACGAAGCGCTCTACCAACACGGGTAA

Upstream 100 bases:

>100_bases
CCCGCGCTAAACTGGCGGCCATGCGCGAGCGTGCCTCCGGCCCAAGCCTGGCCCGCTCTTTACCATCGCGATGCCGCCTG
CCGGCATTCCAAGCACGTGA

Downstream 100 bases:

>100_bases
GCCGTCGGCGCGGCGGTTTGACTCGGCCGCGCCGGCCTGTTAGCTTGGACTGGACGCGGCCGGCATGCCGCGCTGCGCCC
GATCGCTTTCTTTTCAATAT

Product: MFS family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MTMTDSRSTPLTRSDYQTLGLAALGGALEIYDFIIFAFFATTLSKLFFPPDMPEWLRLLQTFGIFATGYLARPLGGILMA
HYADRLGRKRVFSLTILMMAGPCLLIGLMPTYADIGILAPLLLLSLRILQGAAVGGEVPSAWSFVAEHAPRGRRGYALGV
LQAGLTFGYLLGALTATLLARVFTPQEMLDYGWRIPFLLGGPFGLIGVWLRRWLSETPVFLALRERRDARAAYPLLAVLR
RHKHTLLPAAVLTCVLTSAVVVLVVVTPTLLQQRFGMHADRAFALSSVGIVFLNVGCVIAGHVCDRIGQRRSLVLYSLLL
PIGIAALYADLIAGWGWPGLAYAFAGLCCGIVGVVPSIMVGMFPAEVRVSGISFTYNVAYSAWASVTPLLLIAAMPWSPW
ICVLYCATMALTGLFCLRSARNTQEPSLDEALYQHG

Sequences:

>Translated_436_residues
MTMTDSRSTPLTRSDYQTLGLAALGGALEIYDFIIFAFFATTLSKLFFPPDMPEWLRLLQTFGIFATGYLARPLGGILMA
HYADRLGRKRVFSLTILMMAGPCLLIGLMPTYADIGILAPLLLLSLRILQGAAVGGEVPSAWSFVAEHAPRGRRGYALGV
LQAGLTFGYLLGALTATLLARVFTPQEMLDYGWRIPFLLGGPFGLIGVWLRRWLSETPVFLALRERRDARAAYPLLAVLR
RHKHTLLPAAVLTCVLTSAVVVLVVVTPTLLQQRFGMHADRAFALSSVGIVFLNVGCVIAGHVCDRIGQRRSLVLYSLLL
PIGIAALYADLIAGWGWPGLAYAFAGLCCGIVGVVPSIMVGMFPAEVRVSGISFTYNVAYSAWASVTPLLLIAAMPWSPW
ICVLYCATMALTGLFCLRSARNTQEPSLDEALYQHG
>Mature_435_residues
TMTDSRSTPLTRSDYQTLGLAALGGALEIYDFIIFAFFATTLSKLFFPPDMPEWLRLLQTFGIFATGYLARPLGGILMAH
YADRLGRKRVFSLTILMMAGPCLLIGLMPTYADIGILAPLLLLSLRILQGAAVGGEVPSAWSFVAEHAPRGRRGYALGVL
QAGLTFGYLLGALTATLLARVFTPQEMLDYGWRIPFLLGGPFGLIGVWLRRWLSETPVFLALRERRDARAAYPLLAVLRR
HKHTLLPAAVLTCVLTSAVVVLVVVTPTLLQQRFGMHADRAFALSSVGIVFLNVGCVIAGHVCDRIGQRRSLVLYSLLLP
IGIAALYADLIAGWGWPGLAYAFAGLCCGIVGVVPSIMVGMFPAEVRVSGISFTYNVAYSAWASVTPLLLIAAMPWSPWI
CVLYCATMALTGLFCLRSARNTQEPSLDEALYQHG

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=385, Percent_Identity=32.4675324675325, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1788292, Length=445, Percent_Identity=28.9887640449438, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI1789941, Length=437, Percent_Identity=30.6636155606407, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1788942, Length=392, Percent_Identity=31.3775510204082, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI87082231, Length=442, Percent_Identity=23.3031674208145, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87082404, Length=318, Percent_Identity=23.5849056603774, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47392; Mature: 47261

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCHHHHHHHCC
>Mature Secondary Structure 
TMTDSRSTPLTRSDYQTLGLAALGGALEIYDFIIFAFFATTLSKLFFPPDMPEWLRLLQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
TFGIFATGYLARPLGGILMAHYADRLGRKRVFSLTILMMAGPCLLIGLMPTYADIGILAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLLSLRILQGAAVGGEVPSAWSFVAEHAPRGRRGYALGVLQAGLTFGYLLGALTATLLA
HHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RVFTPQEMLDYGWRIPFLLGGPFGLIGVWLRRWLSETPVFLALRERRDARAAYPLLAVLR
HHCCHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RHKHTLLPAAVLTCVLTSAVVVLVVVTPTLLQQRFGMHADRAFALSSVGIVFLNVGCVIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
GHVCDRIGQRRSLVLYSLLLPIGIAALYADLIAGWGWPGLAYAFAGLCCGIVGVVPSIMV
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
GMFPAEVRVSGISFTYNVAYSAWASVTPLLLIAAMPWSPWICVLYCATMALTGLFCLRSA
HCCCHHHEEECEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
RNTQEPSLDEALYQHG
CCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]