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The map label for this gene is guaA

Identifier: 34498920

GI number: 34498920

Start: 3759515

End: 3761092

Strand: Direct

Name: guaA

Synonym: CV_3465

Alternate gene names: 34498920

Gene position: 3759515-3761092 (Clockwise)

Preceding gene: 34498919

Following gene: 34498921

Centisome position: 79.13

GC content: 64.32

Gene sequence:

>1578_bases
ATGGACAAGATCCTCATTCTCGACTTCGGCTCCCAGGTCACCCAGCTGATCGCCCGCCGCGTACGCGAAGCTCACGTTTA
CTGTGAGCTTCATTCTTTCGACATGCCCATCGAAGAGATCCAGGCCTTCGCGCCCAAGGCCATCATCCTGTCCGGCGGCC
CCAACTCGGTGTATGAATCCGACTACCAGGCCGATCCCAAGCTGTTCGAGCTGGGCGTGCCGGTGCTGGGCATCTGCTAC
GGCATGCAGTTCATGGCCCAGACCCTGGGCGGCAAGGTGGAAAGCGGCGACAAGCGCGAGTTCGGCTACGCCCAGATCAA
GGCCCGCCACCACTCCAAGCTGCTGGAAGGCCTGCAGGACCAGATCGACGACGCCGGCAACGGCTTCCTCGACGTGTGGA
TGAGCCACGGCGACAAGGTGACCCAGCTTCCGGCCGGCTTCCAGGTGATCGCCGAGACCCCGTCCTGCCCGTACGCGGCG
ATGGCTGACGAAGCCCGCGGCTTCTACGGCGTGCAGTTCCACCCGGAAGTGACCCACACCAAGCGCGGCACCGAGATGAT
CCACCGCTTCGTGCTGCACGTGGCCGGCTGCAAGCCCAGCTGGACCATGCCCAACTACATCGACGAAGCGGTGAAGAAGA
TCCGCGAGCAAGTCGGCGACGAAGAAGTCATCCTCGGCCTGTCCGGCGGCGTGGACTCCTCGGTGGCCGCCGCGCTGATC
CACCGCGCCATCGGCGACCAGCTGACCTGCGTCTTCGTCGACCACGGCCTGCTGCGCCTGAACGAAGGCAAGATGGTGAT
GGAAATGTTCGCCCAGAACCTGGGCGTCAAGGTGGTCCACGTGCAGGCCGAGGCCGACTTCATGGCCAAGCTGGCCGGCG
AGACCGACCCGGAGAAGAAGCGCAAGATCATCGGCGCGGAATTCATCGAAGTGTTCGACCGCGAATCCGGCAAGCTGACC
AACGCCAAGTGGCTGGCCCAGGGCACCATCTATCCGGACGTGATCGAATCGGCCGGCGCCAAGACCAAGAAGGCGCACAC
CATCAAGAGCCACCACAATGTGGGCGGCCTGCCGGAAGACATGAACCTGAAGCTCTTGGAGCCGCTGCGCGAGCTGTTCA
AGGACGAAGTGCGCCAGCTGGGCGTAGCGCTCGGCCTGCCGCACGACATGGTCTACCGCCACCCGTTCCCGGGCCCGGGC
CTGGGCGTGCGCATCCTCGGCGAAGTGAAGAAGGAATACGCGGATCTCTTGCGCCAGGCCGACGCCATCTTCATCGAAGA
GCTGCGCAACACCGTGGACGAGAAGACCGGCAAAAACTGGTACGAGCTGACCAGCCAGGCCTTCGCCGTGTTCCTGCCGG
TGAAGTCCGTCGGCGTGATGGGCGACGGCCGCACCTACGACTACGTGGTGGCATTGCGCGCCGTGGTGACCAGCGACTTC
ATGACCGCGCACTGGGCCGAGCTGCCGTACAGCCTGCTGGGCCGCGCCTCCAACCGCATCATCAACGAAGTCCGCGGCAT
CAACCGCGTGGTCTACGACGTCAGCGGCAAGCCGCCAGCGACCATCGAGTGGGAATAA

Upstream 100 bases:

>100_bases
CGGCTGCCGCCCAGCGAAGACTGAGCGCGGAAAGCGCGGAATAATTTTCAGCATTGACGATAATGGCCTTAACGGCCTGC
ATTACTTTTCAAGGATAGGC

Downstream 100 bases:

>100_bases
ACCATGCGCAGGCGGCCTCGACCGCCTGACTCCGCCAACAGGCCGACCCCGCGTCGGCCTTTTTCATGCTTGCCTGATGC
CTCGCCCAACACCGCCAGGC

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPIEEIQAFAPKAIILSGGPNSVYESDYQADPKLFELGVPVLGICY
GMQFMAQTLGGKVESGDKREFGYAQIKARHHSKLLEGLQDQIDDAGNGFLDVWMSHGDKVTQLPAGFQVIAETPSCPYAA
MADEARGFYGVQFHPEVTHTKRGTEMIHRFVLHVAGCKPSWTMPNYIDEAVKKIREQVGDEEVILGLSGGVDSSVAAALI
HRAIGDQLTCVFVDHGLLRLNEGKMVMEMFAQNLGVKVVHVQAEADFMAKLAGETDPEKKRKIIGAEFIEVFDRESGKLT
NAKWLAQGTIYPDVIESAGAKTKKAHTIKSHHNVGGLPEDMNLKLLEPLRELFKDEVRQLGVALGLPHDMVYRHPFPGPG
LGVRILGEVKKEYADLLRQADAIFIEELRNTVDEKTGKNWYELTSQAFAVFLPVKSVGVMGDGRTYDYVVALRAVVTSDF
MTAHWAELPYSLLGRASNRIINEVRGINRVVYDVSGKPPATIEWE

Sequences:

>Translated_525_residues
MDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPIEEIQAFAPKAIILSGGPNSVYESDYQADPKLFELGVPVLGICY
GMQFMAQTLGGKVESGDKREFGYAQIKARHHSKLLEGLQDQIDDAGNGFLDVWMSHGDKVTQLPAGFQVIAETPSCPYAA
MADEARGFYGVQFHPEVTHTKRGTEMIHRFVLHVAGCKPSWTMPNYIDEAVKKIREQVGDEEVILGLSGGVDSSVAAALI
HRAIGDQLTCVFVDHGLLRLNEGKMVMEMFAQNLGVKVVHVQAEADFMAKLAGETDPEKKRKIIGAEFIEVFDRESGKLT
NAKWLAQGTIYPDVIESAGAKTKKAHTIKSHHNVGGLPEDMNLKLLEPLRELFKDEVRQLGVALGLPHDMVYRHPFPGPG
LGVRILGEVKKEYADLLRQADAIFIEELRNTVDEKTGKNWYELTSQAFAVFLPVKSVGVMGDGRTYDYVVALRAVVTSDF
MTAHWAELPYSLLGRASNRIINEVRGINRVVYDVSGKPPATIEWE
>Mature_525_residues
MDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPIEEIQAFAPKAIILSGGPNSVYESDYQADPKLFELGVPVLGICY
GMQFMAQTLGGKVESGDKREFGYAQIKARHHSKLLEGLQDQIDDAGNGFLDVWMSHGDKVTQLPAGFQVIAETPSCPYAA
MADEARGFYGVQFHPEVTHTKRGTEMIHRFVLHVAGCKPSWTMPNYIDEAVKKIREQVGDEEVILGLSGGVDSSVAAALI
HRAIGDQLTCVFVDHGLLRLNEGKMVMEMFAQNLGVKVVHVQAEADFMAKLAGETDPEKKRKIIGAEFIEVFDRESGKLT
NAKWLAQGTIYPDVIESAGAKTKKAHTIKSHHNVGGLPEDMNLKLLEPLRELFKDEVRQLGVALGLPHDMVYRHPFPGPG
LGVRILGEVKKEYADLLRQADAIFIEELRNTVDEKTGKNWYELTSQAFAVFLPVKSVGVMGDGRTYDYVVALRAVVTSDF
MTAHWAELPYSLLGRASNRIINEVRGINRVVYDVSGKPPATIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=562, Percent_Identity=36.2989323843416, Blast_Score=308, Evalue=6e-84,
Organism=Escherichia coli, GI1788854, Length=524, Percent_Identity=65.2671755725191, Blast_Score=712, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71992710, Length=575, Percent_Identity=36.8695652173913, Blast_Score=303, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI71992717, Length=575, Percent_Identity=36.8695652173913, Blast_Score=303, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI133901714, Length=575, Percent_Identity=36.8695652173913, Blast_Score=302, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6323873, Length=533, Percent_Identity=51.219512195122, Blast_Score=534, Evalue=1e-152,
Organism=Drosophila melanogaster, GI281365319, Length=560, Percent_Identity=35.7142857142857, Blast_Score=299, Evalue=4e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_CHRVO (Q7NSG1)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903135.1
- ProteinModelPortal:   Q7NSG1
- SMR:   Q7NSG1
- GeneID:   2547643
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3465
- NMPDR:   fig|243365.1.peg.3465
- HOGENOM:   HBG391775
- OMA:   MKLVEPL
- PhylomeDB:   Q7NSG1
- ProtClustDB:   PRK00074
- BioCyc:   CVIO243365:CV_3465-MONOMER
- BRENDA:   6.3.5.2
- HAMAP:   MF_00344
- InterPro:   IPR006220
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00097
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase

EC number: =6.3.5.2

Molecular weight: Translated: 58265; Mature: 58265

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 79-79 ACT_SITE 174-174 ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPIEEIQAFAPKAIILSGGPNSVYES
CCEEEEEECCHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHCCCEEEEECCCCCCCCC
DYQADPKLFELGVPVLGICYGMQFMAQTLGGKVESGDKREFGYAQIKARHHSKLLEGLQD
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
QIDDAGNGFLDVWMSHGDKVTQLPAGFQVIAETPSCPYAAMADEARGFYGVQFHPEVTHT
HHHCCCCCEEEHHHHCCCCEEECCCCCEEEECCCCCCCHHHHHHCCCEEEEEECCCCCCC
KRGTEMIHRFVLHVAGCKPSWTMPNYIDEAVKKIREQVGDEEVILGLSGGVDSSVAAALI
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
HRAIGDQLTCVFVDHGLLRLNEGKMVMEMFAQNLGVKVVHVQAEADFMAKLAGETDPEKK
HHHHCCCEEEEEEECCEEEECCCHHHHHHHHHHCCEEEEEEECCHHHHHHHCCCCCHHHH
RKIIGAEFIEVFDRESGKLTNAKWLAQGTIYPDVIESAGAKTKKAHTIKSHHNVGGLPED
HHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCC
MNLKLLEPLRELFKDEVRQLGVALGLPHDMVYRHPFPGPGLGVRILGEVKKEYADLLRQA
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DAIFIEELRNTVDEKTGKNWYELTSQAFAVFLPVKSVGVMGDGRTYDYVVALRAVVTSDF
HHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHH
MTAHWAELPYSLLGRASNRIINEVRGINRVVYDVSGKPPATIEWE
HHHHHHHCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPIEEIQAFAPKAIILSGGPNSVYES
CCEEEEEECCHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHCCCEEEEECCCCCCCCC
DYQADPKLFELGVPVLGICYGMQFMAQTLGGKVESGDKREFGYAQIKARHHSKLLEGLQD
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
QIDDAGNGFLDVWMSHGDKVTQLPAGFQVIAETPSCPYAAMADEARGFYGVQFHPEVTHT
HHHCCCCCEEEHHHHCCCCEEECCCCCEEEECCCCCCCHHHHHHCCCEEEEEECCCCCCC
KRGTEMIHRFVLHVAGCKPSWTMPNYIDEAVKKIREQVGDEEVILGLSGGVDSSVAAALI
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
HRAIGDQLTCVFVDHGLLRLNEGKMVMEMFAQNLGVKVVHVQAEADFMAKLAGETDPEKK
HHHHCCCEEEEEEECCEEEECCCHHHHHHHHHHCCEEEEEEECCHHHHHHHCCCCCHHHH
RKIIGAEFIEVFDRESGKLTNAKWLAQGTIYPDVIESAGAKTKKAHTIKSHHNVGGLPED
HHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCC
MNLKLLEPLRELFKDEVRQLGVALGLPHDMVYRHPFPGPGLGVRILGEVKKEYADLLRQA
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DAIFIEELRNTVDEKTGKNWYELTSQAFAVFLPVKSVGVMGDGRTYDYVVALRAVVTSDF
HHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHH
MTAHWAELPYSLLGRASNRIINEVRGINRVVYDVSGKPPATIEWE
HHHHHHHCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782