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The map label for this gene is eno [H]

Identifier: 34498914

GI number: 34498914

Start: 3754880

End: 3755488

Strand: Direct

Name: eno [H]

Synonym: CV_3459

Alternate gene names: 34498914

Gene position: 3754880-3755488 (Clockwise)

Preceding gene: 34498912

Following gene: 34498915

Centisome position: 79.03

GC content: 62.07

Gene sequence:

>609_bases
GTGGACGCCGCCGGCTACGTGGCCGGCCAGGACGTGATGATCGCGCTGGACTGCGCCGCCTCCGAGTTCTTCCGCGACGG
CAAGTACCACCTGACCGCCGAAAAACTGTCGCTGTCCTCGGAAGAGTTCGCCGACTACCTGGAAAACCTGGTCAACAAAT
ACCCGATCATCTCGATCGAGGATGGCATGGCCGAGCAGGACTGGGCAGGCTGGAAGCTGCTGACCGACCGCCTGGGCCAG
AAGGTGCAGATCGTCGGCGACGACGTGTTCGTCACCAACCCCAAGATTCTGGCCGAAGGCATCGCCAAGGGCGTGGCCAA
CTCGCTGCTGGTGAAGGTCAACCAGATCGGCACGCTGTCGGAAACCCTGAAAGCAGTTGATCTGGCCAAGCGTTCCCGCT
ATACCTGCGTTATGAGCCACCGCTCCGGCGAGACCGAGGACAGCACCATCGCCGATCTGGCAGTCGCCACCAACTGCATG
CAGATCAAGACCGGTTCGCTGTCGCGCTCCGACCGCATGGCCAAGTACAACCAACTGCTGCGCATCGAGGAAGAACTGGG
CGACGCCGCCCACTACCCCGGCCGTGCGGCTTTCTACCATCTGAAGTAA

Upstream 100 bases:

>100_bases
TGCGACCGCCACGGCTACGCCACCACCGTCGGCGACGAGGGCCGGCTTCGCGCCGAACCTGGAAAGCCACGAAGACGCGA
TCAAGCTGATCCTGGAAGCC

Downstream 100 bases:

>100_bases
TTGCATGCGCTGGCTGACCGTGACCCTGGTAACCGTGATCCTCGCCCTGCAATGGCCGCTTTGGTTCGGCAAGGGCAGTT
GGCTCAGGGTCTGGCAGCTG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MDAAGYVAGQDVMIALDCAASEFFRDGKYHLTAEKLSLSSEEFADYLENLVNKYPIISIEDGMAEQDWAGWKLLTDRLGQ
KVQIVGDDVFVTNPKILAEGIAKGVANSLLVKVNQIGTLSETLKAVDLAKRSRYTCVMSHRSGETEDSTIADLAVATNCM
QIKTGSLSRSDRMAKYNQLLRIEEELGDAAHYPGRAAFYHLK

Sequences:

>Translated_202_residues
MDAAGYVAGQDVMIALDCAASEFFRDGKYHLTAEKLSLSSEEFADYLENLVNKYPIISIEDGMAEQDWAGWKLLTDRLGQ
KVQIVGDDVFVTNPKILAEGIAKGVANSLLVKVNQIGTLSETLKAVDLAKRSRYTCVMSHRSGETEDSTIADLAVATNCM
QIKTGSLSRSDRMAKYNQLLRIEEELGDAAHYPGRAAFYHLK
>Mature_202_residues
MDAAGYVAGQDVMIALDCAASEFFRDGKYHLTAEKLSLSSEEFADYLENLVNKYPIISIEDGMAEQDWAGWKLLTDRLGQ
KVQIVGDDVFVTNPKILAEGIAKGVANSLLVKVNQIGTLSETLKAVDLAKRSRYTCVMSHRSGETEDSTIADLAVATNCM
QIKTGSLSRSDRMAKYNQLLRIEEELGDAAHYPGRAAFYHLK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI4503571, Length=197, Percent_Identity=48.2233502538071, Blast_Score=185, Evalue=3e-47,
Organism=Homo sapiens, GI5803011, Length=199, Percent_Identity=46.7336683417085, Blast_Score=184, Evalue=4e-47,
Organism=Homo sapiens, GI301897479, Length=200, Percent_Identity=46.5, Blast_Score=181, Evalue=3e-46,
Organism=Homo sapiens, GI301897477, Length=200, Percent_Identity=46.5, Blast_Score=181, Evalue=3e-46,
Organism=Homo sapiens, GI301897469, Length=200, Percent_Identity=46.5, Blast_Score=181, Evalue=3e-46,
Organism=Homo sapiens, GI169201331, Length=186, Percent_Identity=31.7204301075269, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI169201757, Length=186, Percent_Identity=31.7204301075269, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI239744207, Length=186, Percent_Identity=31.7204301075269, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI310129182, Length=125, Percent_Identity=32, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI310110045, Length=125, Percent_Identity=32, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI310120572, Length=125, Percent_Identity=32, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1789141, Length=194, Percent_Identity=63.4020618556701, Blast_Score=258, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI71995829, Length=201, Percent_Identity=45.771144278607, Blast_Score=176, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI17536383, Length=201, Percent_Identity=45.771144278607, Blast_Score=176, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=46.3157894736842, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6321968, Length=201, Percent_Identity=51.7412935323383, Blast_Score=189, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6321693, Length=201, Percent_Identity=50.7462686567164, Blast_Score=189, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6323985, Length=190, Percent_Identity=47.8947368421053, Blast_Score=176, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6324974, Length=190, Percent_Identity=47.8947368421053, Blast_Score=176, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6324969, Length=190, Percent_Identity=47.8947368421053, Blast_Score=176, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24580918, Length=199, Percent_Identity=44.7236180904523, Blast_Score=171, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24580916, Length=199, Percent_Identity=44.7236180904523, Blast_Score=171, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24580920, Length=199, Percent_Identity=44.7236180904523, Blast_Score=171, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24580914, Length=199, Percent_Identity=44.7236180904523, Blast_Score=171, Evalue=4e-43,
Organism=Drosophila melanogaster, GI281360527, Length=199, Percent_Identity=44.7236180904523, Blast_Score=171, Evalue=4e-43,
Organism=Drosophila melanogaster, GI17137654, Length=199, Percent_Identity=44.7236180904523, Blast_Score=171, Evalue=4e-43,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 22243; Mature: 22243

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAAGYVAGQDVMIALDCAASEFFRDGKYHLTAEKLSLSSEEFADYLENLVNKYPIISIE
CCCCCCCCCCCEEEEEEHHHHHHHCCCCEEEEHHHHCCCHHHHHHHHHHHHHHCCEEEEC
DGMAEQDWAGWKLLTDRLGQKVQIVGDDVFVTNPKILAEGIAKGVANSLLVKVNQIGTLS
CCCCCCCCCCHHHHHHHCCCEEEEEECCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCHH
ETLKAVDLAKRSRYTCVMSHRSGETEDSTIADLAVATNCMQIKTGSLSRSDRMAKYNQLL
HHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHH
RIEEELGDAAHYPGRAAFYHLK
HHHHHHCCCCCCCCCCEEEECC
>Mature Secondary Structure
MDAAGYVAGQDVMIALDCAASEFFRDGKYHLTAEKLSLSSEEFADYLENLVNKYPIISIE
CCCCCCCCCCCEEEEEEHHHHHHHCCCCEEEEHHHHCCCHHHHHHHHHHHHHHCCEEEEC
DGMAEQDWAGWKLLTDRLGQKVQIVGDDVFVTNPKILAEGIAKGVANSLLVKVNQIGTLS
CCCCCCCCCCHHHHHHHCCCEEEEEECCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCHH
ETLKAVDLAKRSRYTCVMSHRSGETEDSTIADLAVATNCMQIKTGSLSRSDRMAKYNQLL
HHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHH
RIEEELGDAAHYPGRAAFYHLK
HHHHHHCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA